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Improvement of experimental animal handling skills for higher education laboratory technicians in Semarang Region Indonesia Aditya Marianti; Wulan Christijanti; Wiwi Isnaeni; Badingatus Sholihah; Kartika Widyaningrum; Naufal Sebastian Anggoro; Yudi Priyanto
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jp.v5i1.3670

Abstract

Laboratory technician skills in handling experimental animals are needed by the study program, which uses experimental animals in the lecture and research process. The Biology Laboratory of FMIPA UNNES has the resources to practice these skills. As the realization of the 3rd Dharma of the Tri Dharma of Higher Education, community service is carried out to improve the skills of higher education laboratory technicians both private and public universities in the city of Semarang in handling experimental animals. The method used is training in the form of hands-on practice. The animals used were mice and white rats. The trained practices were bioethics, maintenance techniques, handling, gavage, drawing blood, and vaginal smears of experimental animals. The participants consisted of 29 laboratory technicians from general medicine, dentistry, biology, biology education, pharmacy, and animal husbandry study programs. The training instructors consist of lecturers, technicians, and student assistants. The training was held for two days by implementing health protocols. As a result, the participants improved their skills. From the beginning, only 29,26% of participants had the knowledge and skills to handle experimental animals at the end of the training, increasing to 86.84%. It concludes that the participants have increased their knowledge and skills in handling experimental animals.
Pengembangan Taman TOGA Untuk Peningkatan Kesehatan Warga Panti Asuhan Yafikhi Semarang R. Susanti; Wulan Christijanti; Noor Aini Habibah; Dewi Mustikaningtyas; Fitri Arum Sasi; Damar Galih; Sasabila Mumtaz; Anisa Dewi Sri Puspitasari
ABDIMASKU : JURNAL PENGABDIAN MASYARAKAT Vol 6, No 1 (2023): Januari 2023
Publisher : LPPM UNIVERSITAS DIAN NUSWANTORO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33633/ja.v6i1.1066

Abstract

Panti Asuhan Yafikhi memiliki lahan kosong yang berpotensi digunakan sebagai perkebunan. Tantangan dalam pengembangan pertanian hortikultura adalah keseimbangan penggunaan bahan organik dan anorganik. Tujuan dari kegiatan pengabdian ini adalah untuk memberdayakan warga panti asuhan Yafikhi dalam (1) merancang arsitektur landscape “Taman TOGA” sesuai dengan luas dan kondisi lahan, (2) mengumpulkan dan menanam toga sesuai dengan landscape yang telah dibuat, (3) Menerapkan bioteknologi dalam pemanfaatan limbah rumah tangga menjadi pupuk organik, (4) merawat dan mengelola kebun TOGA menggunakan pupuk organik, (5) memanfaatkan tanaman obat untuk menjaga kesehatan. Metode yang digunakan adalah workshop, praktik, dan pendampingan. Hasil pengabdian menunjukkan peningkatan pemahaman warga mitra (Panti Asuhan Yafikhi Semarang) tentang pentingnya tanaman obat untuk menjaga kesehatan, terampil merancang dan mewujudkan Taman TOGA, terampil mengelola sampah rumah tangga menjadi kompos padat dan pupuk organik cair, dan terampil menggunakan pupuk organik untuk memelihara taman TOGA.
PERANAN MULTIMEDIADALAMMENINGKATKAN PEMAHAMAN MAHASISWAPADA MATA KULIAH BIOLOGI SEL Sumad Sumad; Wulan Christijanti; Aditya Marianti
Jurnal Penelitian Pendidikan Vol 28, No 1 (2011): April 2011
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpp.v28i1.5616

Abstract

Cell Biology material that can not be seen with the naked eye and had to use the help tools (microscope) to be one reason for student difficulties in imagining is real. Time of lessons a limited cause not all the material can be given during lectures. Also by means of a simple (OHP) makes the material can not be described in detail. These facts affect the learning results obtained is not optimal, that is only 55% of students who obtained a value ≥ 70. Hence the need for a multimedia tool that is able to present the text, images, graphics, video, sound, and animation, so that students will gain knowledge as well as a concrete experience .The purpose of this study were: to determine the utilization of multimedia in Cell Biology course in improving student understanding. This research was conducted in 2 cycles with each cycle comprising three meetings. The first and second meetings for learning with multimedia and the third meeting was the discussion and evaluation. The main variables are the result of learning by supporting variables, such as student activity during the discussion, the performance of lecture and student responses . As an indicator of student performance is the result of learning gained ≥ 71 ≥ 80% of the whole class. The results showed in the first cycle the average results of student learning has reached 77.33 with the understanding level of 72.97%. The average study result on the second cycle is 81.77 with a level of understanding of 94.59%. To support variable (cycle I and II), ≥ 55% - 100% of students have been conducting activities during the discussion, score 77% - 100% for the performance of lecture and student responses is positive. Conclusion drawn is that the Cell Biology of learning with multimedia can increase students' understanding. This is indicated by the average student learning outcomes is 77.33 with a level of understanding by 72, 97% in the first cycle increased on the second cycle was increased to 81.77 with a level of understanding reached 94.59%. Suggestions put forward is that it took a relatively long time in preparing a variety of research instruments and time efficiency in teaching and learning.
EFEKTIVITAS PEMBERIAN MINYAK SAWIT DAN MINYAK LEMURU DALAM MEMPERCEPAT PUBERTAS TIKUS BETINA Mirtaati Na’ima; R. Susanti; Wulan Christijanti
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2266

Abstract

Penelitian ini bertujuan untuk mengkaji efektivitas pemberian minyak sawit dan minyak lemuru dalam mempercepat pubertas tikus betina, pada parameter peningkatan berat badan, berat ovarium dan perkembangan folikel ovarium. Tikus betina usia 21 hari sebanyak 20 ekor dibagi menjadi 4 kelompok variasi pemberian minyak sawit dan minyak lemuru per oral, yaitu kelompok A (0%+0%), B (3%+3%), C (4%+4%), dan D (5%+5%). Pada akhir penelitian (hari ke-20), dilakukan pengambilan data peningkatan berat badan, berat ovarium, dan perkembangan folikel ovarium. Data dianalisis secara deskripsi dan statistik dengan anava satu arah. Jika terdapat perbedaan, dilanjutkan dengan uji Beda Nyata Terkecil. Hasil penelitian ini menunjukkan bahwa minyak sawit dan minyak lemuru dapat mempercepat pubertas tikus betina khususnya dari peningkatan berat badan, berat ovarium, dan perkembangan folikel ovarium. Dosis paling efektif untuk mempercepat pubertas adalah 4% minyak sawit + 4% minyak lemuru. This study was aimed to examine the effectiveness of palm and lemuru oil for the acceleration of female rat puberty, especially in increasing the body weight, the ovarian weight and the follicle development. Twenty female rats aged 21 days were divided into 4 based on the variation of orally feeding palm and lemuru oil, namely group A (0% +0%), B (3% +3%), C (4% +4%), and D (5% +5%). At the twentieth day, data of the increase of the body weight, the ovarian weight and the follicle development were collected and then analyzed descriptively and statistically using one-way ANOVA. For any difference, the Least Significance Difference Test would be performed. The result of this study showed that palm and lemuru oil can accelerate the puberty of female rats. The most effective dose was 4% palm oil and 4% lemuru oil.
Intestinal Protective Efficacy of Gamma Co-60 Irradiated Chitosan and Vitamin E Combination on Lead acetate-induced Rats Aditya Marianti; Edward Louis Krey; Wulan Christijanti; Lisdiana Lisdiana
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 1 (2022): April 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i1.34822

Abstract

As an organ that absorbs food, the intestine can be damaged when interacting directly with lead (Pb). Gamma Co-60 irradiated chitosan, and vitamin E combination potentially can help reduce free radical damage to intestinal organs. This study examines the efficacy of gamma Co-60 irradiated chitosan and vitamin E combination in protecting the intestinal tissue of rats exposed to Pb acetate. The 24 rats were grouped into 6 groups, each 4 rats; were induced with Pb acetate, except the control group. The negative control group was only induced Pb acetate. The positive control group had additional treatment of irradiated chitosan. The treatment group (1-3) was treated using the combination of gamma Co-60 irradiated chitosan at a dose of 64 mg/kg BW and vitamin E 1000 IU at a dose of 1.44; 2.16, 3.00 mg/kg BW, respectively, for forty days. The research parameters observed are edema, degeneration, necrosis, erosion of the intestinal villi, and lysis of the intestinal villi. Intestinal histology appearance data in all groups were analyzed descriptively. The result shows that a combination of Gamma Co-60 irradiated chitosan at a dose of 64 mg/kg BW and vitamin E at a dose of 1.44 mg/kg BW effectively reduced lead cytotoxicity in the intestinal tissue of Pb acetate-induced rats. The larger the dose of vitamin E has the potential to produce pro-oxidants, which will increase the toxic effect on intestinal tissue. Gamma Co-60 irradiated chitosan, and vitamin E combination at the right dose has the potential to be developed as a supplement to reduce the toxic effects of chronic lead exposure. Especially for people who, for some reason, cannot avoid chronic lead exposure. 
Efek Pemberian Antioksidan Vitamin C dan E terhadap Kualitas Spermatozoa Tikus Putih Terpapar Allethrin Wulan Christijanti; Nur Rahayu Utami; Arya Iswara
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1148

Abstract

Allethrin  is  one  of  synthetic  pyrethroid types which  is  used  in many mosquito repellents and has toxic potential. The aim of this research was to examine the infl uence of vitamin C and E antioxidant activity to the sperm quality of allethrin-induced albino rats. This research used a post-test randomized design. The population of the research were  8  hr/day  allethrin-induced male  albino  rats. The  sample  of  the  research was allethrin-induced male albino rats which were divided into 4 groups, i.e. group I was the control group, group II was the group treated with 1.8 mg vitamin C per day, group II was the group treated with 1.44 mg vitamin E per day, and group IV was treated with a combination of 1.8 mg vitamin C per day and 1.44 mg vitamin E per day. The duration of the treatment was 45 days. The sperm sample was taken in day 46. The data consisted of sperm count or amount, sperm motility, sperm viability, and sperm morphology, and then was analyzed with ANOVA followed by BNT. The result showed that vitamin C and E antioxidants signifi cantly infl uenced the the sperm quality of the 45-day mosquito repellent-induced rats. Overall, the allethrin-induced albino rats treated with vitamin C and E antioxidants were better in maintaining the sperm quality than single treatment of vitamin antioxidants.Keywords: allethrin, vitamin C, vitamin E, antioxidant, sperm  quality.
The Effect Of Moringa Leaf Extract On Hyperglycemic Rat Liver Function Wulan Christijanti; Aditya Marianti; R. Susanti; Senda Kartika Rakainsa
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 2 (2022): August 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i2.35431

Abstract

One of the management of diabetics is to maintain stable glucose levels. Often diabetes treatment combines chemical drugs with medicinal plants. People have consumed a lot of Moringa leaves which are believed to be able to maintain body condition. One of the benefits of Moringa leaves with phytochemical components in it is as a hepatoprotector. The aim of this study was to analyze the role of Moringa leaf extract on liver function parameters of hyperglycemic rats. There were 4 groups, normal control (K0), hyperglycemic rats with induction of alloxan 125 mg/kg bw and divided into three groups treated with moringa leaf extract at a dose of 0 mg (K1), 200 mg (T1) and 400 mg/kg bw (T2 ) for 21 days. The variables measured were aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, total cholesterol. Data were analyzed with Anova P0.05 and Least Significance Different (LSD) follow-up test. The results showed that the levels of AST and ALT were highest in the K1 group which then decreased significantly in the group that received Moringa extract (T1 and T2). Statistically there was a significant difference (p0.05) between the control and treatment groups. Cholesterol levels in the control group were significantly lower than the diabetes and treatment groups, but there was no significant difference between the treatment groups. The conclusion is that Moringa leaf extract has an effect on reducing liver enzyme levels and cholesterol in hyperglycemic rats. This research adds to the study of the hepatoprotective potential of Moringa leaf extract in hyperglycemic rats.
EFEK EKSTRAK DAUN TEH HIJAU (Camelia sinensis L) TERHADAP STRUKTUR HISTOLOGIS HEPAR TIKUS DIINDUKSI PARASETAMOL M.A. Sunny; Wulan Christijanti
Prosiding Seminar Nasional Biologi Vol. 11 (2023)
Publisher : Prosiding Seminar Nasional Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Parasetamol yang dikonsumsi secara berlebihan, dapat menyebabkan kerusakan hepar. Salah satu tanda kerusakan hepar adalah perubahan struktur histologis. Ekstrak teh hijau diyakini mampu bersifat sebagai hepatoprotektor. Penelitian ini bertujuan untuk menganalisis efek ekstrak teh hijau terhadap histologiss hepar tikus diinduksi parasetamol. Tikus jantan sebanyak 25 ekor dibagi menjadi 5 kelompok yaitu K, K+ diinduksi parasetamol 200 mg/kg BB, kelompok perlakuan diinduksi parasetamol 200 mg/kg BB dan ekstrak teh hijau 175 mg (P1), 350 mg (P2), 700 mg/kg BB (P3). Ekstrak teh hijau diberikan selama 14 hari dan pada hari ke 15 diberi parasetamol selama 10 hari. Hepar dibuat preparat histologis dengan metode parafin dan pewarnaan hematoksilin-eosin (HE). Data perubahan struktur berupa degenerasi parenkimatosa, degenerasi hidropik dan nekrosis diamati dengan skoring Manja Roenigk. Data dianalisis menggunakan uji statistic non parametrik Kruskal Wallis dilanjutkan dengan uji Duncan. Pemberian ekstrak teh hijau dosis 175 mg tidak berpengaruh signifikan dalam mencegah perubahan struktur histologis hepar yang disebabkan oleh parasetamol. Kelompok P2 dan P3 memberikan hasil berbeda signifikans dalam mengurangi degenerasi parenkimatosa, degenerasi hidropik dan nekrosis. Disimpulkan, ekstrak teh hijau mampu mencegah perubahan histologis hepar akibat induksi parasetamol.