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ANALISIS KADAR LOGAM BERAT PADA SUNGAI DI JAWA TENGAH Susanti, R. Adi; Mustikaningtyas, Dewi; Sasi, Fitri Arum
Sainteknol : Jurnal Sains dan Teknologi Vol 12, No 1 (2014): June 2014
Publisher : Unnes Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v12i1.5424

Penelitian bertujuan untuk menganalisis kadar cemaran logam berat pada sedimen air sungai di Jawa Tengah (Kaligarang, Juwana, Bengawan Solo, Kali Agung, Kali Banger). Sampel penelitian berupa sedimen sungai. Sampel diuji kadar logam berat (Hg, Cd, Cu, Cr, Zn, Pb). Uji kadar logam berat dilakukan dengan menggunakan metode Mercury analycer (Hg) dan AtomicAbsorptionSpectroscopy (Cd, Cu, Cr, Zn, Pb). Hasil pengujian menunjukkan, kadar logam tertinggi adalah Zn dan Cu. Kadar logam Zn dari sumber cemaran industri makanan, farmasi, rumah tangga, peleburan timah, pembuangan oli, limbah minyak dan limbah batik secara berturut-turut sebesar 254,12 ppm; 211,65 ppm; 151,56 ppm; 112,02 ppm; 124,65 ppm; 127,42 ppm dan 133,417 ppm. Kadar logam Cu dari sumber cemaran yang sama selain limbah farmasi berturut-turut sebesar 49,78 ppm; 30,77 ppm; 30,01 ppm; 27,98 ppm; 16,20 ppm dan 25,70 ppm. Untuk industri farmasi, kadar logam tertinggi kedua adalah Pb yaitu sebesar 57,40 ppm. Disimpulkan bahwa dari semua jenis sumber cemaran yang masuk ke badan air sungai sebagian besar mengandung logam Zn dan Cu. Secara keseluruhan kadar Hg, Cd, Cu, Zn, Cr, dan Pb cukup tinggi. Perairan sungai dalam penelitian ini, pada saat pengamatan menerima masukan limbah yang mengandung Zn lebih banyak dibandingkan logam lain.

Molecular Characterization of Zinc (Zn) Resistant Bacteria in Banger River, Pekalongan, Indonesia Budiharjo, Anto; Kusumaningrum, Hermin Pancasakti; Sasi, Fitri Arum
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Indigenous bacteria are able to remove the metals contamination in environment. This study aimed to assess the resistance of bacterial species to Zinc (Zn) in Banger River, Pekalongan City. The bacteria from three different parts of Banger River were isolated and inoculated in Zn-selective medium. Then, molecular identification to determine the bacteria species was conducted using polymerase chain reaction (PCR) by applying forward-reverse 16SrRNA gene primers. The sequences analysis was conducted using MUSCLE and MEGA6. There were seven dominant species that possibly resistant to Zn. Approximately, every isolate could reach more than 95 % from 2000 ppm of Zn in the medium. The higher absorption of Zn was found in Z5 isolate. The seven bacteria species were clustered into nine genera i.e. Klebsiela, Xenorhabdus, Cronobacter, Enterobacter, Escherichia, Shigella and Sporomusa known as Gram Negative bacteria and Clostridium and Bacillus as Gram Positive bacteria. In Gram Positive bacteria, especially Bacillus sp, carboxyl group in peptidoglycan play a role as metal binder. In Gram-negative bacteria, lipopolysaccharide (LPS) which is highly anionic component on the outer membrane, able to catch the Zn. Besides that, Enterobacter activates endogen antioxidants such as glutathione peroxidase (GSHPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD). The research found there was possible seven novel indigenous bacteria species in Banger that able to remove Zn from the sediment extremely. This finding can be developed as an eco-friendly approach to reduce metals pollution using local microorganisms.

ANALISIS CEK LIST KESELAMATAN DAN KESEHATAN KERJA DI LABORATORIUM BIOLOGI FMIPA UNNES Ria Ika Maharani; Fitri Arum Sasi
Integrated Lab Journal Vol. 6 No. 1 (2018)
Publisher : UIN Sunan Kalijaga Yogyakarta

Occupational safety and health in the laboratory becomes very important considering the laboratory has a high risk of accident. Assessment of the risk of accidents in the laboratory cover many components, such as chemicals, equipment, supporting factors and users. With a high level of risk, the application of occupational safety and health needs to be monitored and assessed. The purpose of this study was to analyze the safety and health of the laboratory in biology based on the check list of safety and health reference. The method used is descriptive in the form of questionnaires with data analysis using categories based on normative values that is x ≥ 67.5 category is very good, 45 ≤ x <67.5 good category, 22.5 ≤ x <45 category good enough and x ≤ 22 , 5 categories are not good. The conclusion of the research is occupational safety and health in the biology laboratory included in the good category with the average score 57.28. This shows that based on the reference matrix of safety and health check list in the laboratory consisting of laboratory layout, office, personal protective equipment, hygiene, emergency tool, chemicals, glassware and electricity have been done well.

ANALISIS KADAR LOGAM BERAT PADA SUNGAI DI JAWA TENGAH Susanti, R. Adi; Mustikaningtyas, Dewi; Sasi, Fitri Arum
Sainteknol : Jurnal Sains dan Teknologi Vol 12, No 1 (2014): June 2014
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v12i1.5424

Penelitian bertujuan untuk menganalisis kadar cemaran logam berat pada sedimen air sungai di Jawa Tengah (Kaligarang, Juwana, Bengawan Solo, Kali Agung, Kali Banger). Sampel penelitian berupa sedimen sungai. Sampel diuji kadar logam berat (Hg, Cd, Cu, Cr, Zn, Pb). Uji kadar logam berat dilakukan dengan menggunakan metode Mercury analycer (Hg) dan AtomicAbsorptionSpectroscopy (Cd, Cu, Cr, Zn, Pb). Hasil pengujian menunjukkan, kadar logam tertinggi adalah Zn dan Cu. Kadar logam Zn dari sumber cemaran industri makanan, farmasi, rumah tangga, peleburan timah, pembuangan oli, limbah minyak dan limbah batik secara berturut-turut sebesar 254,12 ppm; 211,65 ppm; 151,56 ppm; 112,02 ppm; 124,65 ppm; 127,42 ppm dan 133,417 ppm. Kadar logam Cu dari sumber cemaran yang sama selain limbah farmasi berturut-turut sebesar 49,78 ppm; 30,77 ppm; 30,01 ppm; 27,98 ppm; 16,20 ppm dan 25,70 ppm. Untuk industri farmasi, kadar logam tertinggi kedua adalah Pb yaitu sebesar 57,40 ppm. Disimpulkan bahwa dari semua jenis sumber cemaran yang masuk ke badan air sungai sebagian besar mengandung logam Zn dan Cu. Secara keseluruhan kadar Hg, Cd, Cu, Zn, Cr, dan Pb cukup tinggi. Perairan sungai dalam penelitian ini, pada saat pengamatan menerima masukan limbah yang mengandung Zn lebih banyak dibandingkan logam lain.

THE POTENTIAL OF CNIDOSCOLUS CHAYAMANSA ALCHOHOLIC LEAVES EXTRACT AS HYPOLIPIDEMIA AGENT Iswari, Retno Sri; Mubarok, Ibnul; Sasi, Fitri Arum
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Cardiovascular disease caused by hyperlipidemia is the highest case in term of fatality of non-infectious disease in Indonesia. Cnidoscolus chayamansa is well known as a herb that has nutraceutical potential for medicine and likely as a hypolipidemia agent. Therefore, this study aimed to identify the optimal dose of C. chayamansa as a candidate to treat cardiovascular diseases. A total of 30 white rats were acclimatized in the laboratory for 14 days. After that, the rats were grouped into six groups, randomly, which including a healthy control group (K0), a negative control group or hyperlipidemic rats (K-), a positive control group or hyperlipidemia-induced rats that was supplemented with atorvastatin (K+), then a hyperlipidemic rats treated with alcoholic extract of C. chayamansa leaves at doses of 100 mg /kgBB /day for K1, 200 mg/ kgBB/ day for K2 and 400 mg/ KgBB/ day for K3. The results showed that K3 treatment is the most effective and optimum dose for lowering total cholesterol level at 76.81±1.10 mg/dl, triglycerides 72.39±1.66 mg/dl and LDL-C at 21.47±0.58 mg/dl. The aortic histology assessment also showed that the K- group had putative thrombus or plaque in intima, and it was not found in other groups. This research focused on the optimum dose and new usage of C. chayamansa, as an anti-inflammatory in atherosclerosis. By understanding the optimum dose of C. chayamansa, the community can apply and control the herb consumption for their own therapeutic properties. For future application, the herb is potentially developed as anti-atherogenic medicine.

Molecular Characterization of Zinc (Zn) Resistant Bacteria in Banger River, Pekalongan, Indonesia Sasi, Fitri Arum; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.15835

Indigenous bacteria are able to remove the metals contamination in environment. This study aimed to assess the resistance of bacterial species to Zinc (Zn) in Banger River, Pekalongan City. The bacteria from three different parts of Banger River were isolated and inoculated in Zn-selective medium. Then, molecular identification to determine the bacteria species was conducted using polymerase chain reaction (PCR) by applying forward-reverse 16SrRNA gene primers. The sequences analysis was conducted using MUSCLE and MEGA6. There were seven dominant species that possibly resistant to Zn. Approximately, every isolate could reach more than 95 % from 2000 ppm of Zn in the medium. The higher absorption of Zn was found in Z5 isolate. The seven bacteria species were clustered into nine genera i.e. Klebsiela, Xenorhabdus, Cronobacter, Enterobacter, Escherichia, Shigella and Sporomusa known as Gram Negative bacteria and Clostridium and Bacillus as Gram Positive bacteria. In Gram Positive bacteria, especially Bacillus sp, carboxyl group in peptidoglycan play a role as metal binder. In Gram-negative bacteria, lipopolysaccharide (LPS) which is highly anionic component on the outer membrane, able to catch the Zn. Besides that, Enterobacter activates endogen antioxidants such as glutathione peroxidase (GSHPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD). The research found there was possible seven novel indigenous bacteria species in Banger that able to remove Zn from the sediment extremely. This finding can be developed as an eco-friendly approach to reduce metals pollution using local microorganisms.

The Potential of Cnidoscolus chayamansa Alchoholic Leaves Extract as Hypolipidemia Agent Iswari, Retno Sri; Mubarok, Ibnul; Sasi, Fitri Arum
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.23687

Cardiovascular disease caused by hyperlipidemia is the highest case in term of fatality of non-infectious disease in Indonesia. Cnidoscolus chayamansa is well known as a herb that has nutraceutical potential for medicine and likely as a hypolipidemia agent. Therefore, this study aimed to identify the optimal dose of C. chayamansa as a candidate to treat cardiovascular diseases. A total of 30 white rats were acclimatized in the laboratory for 14 days. After that, the rats were grouped into six groups, randomly, which including a healthy control group (K0), a negative control group or hyperlipidemic rats (K-), a positive control group or hyperlipidemia-induced rats that was supplemented with atorvastatin (K+), then a hyperlipidemic rats treated with alcoholic extract of C. chayamansa leaves at doses of 100 mg /kgBB /day for K1, 200 mg/ kgBB/ day for K2 and 400 mg/ KgBB/ day for K3. The results showed that K3 treatment is the most effective and optimum dose for lowering total cholesterol level at 76.81Â±1.10 mg/dl, triglycerides 72.39Â±1.66 mg/dl and LDL-C at 21.47Â±0.58 mg/dl. The aortic histology assessment also showed that the K- group had putative thrombus or plaque in intima, and it was not found in other groups. This research focused on the optimum dose and new usage of C. chayamansa, as an anti-inflammatory in atherosclerosis. By understanding the optimum dose of C. chayamansa, the community can apply and control the herb consumption for their own therapeutic properties. For future application, the herb is potentially developed as anti-atherogenic medicine.

Pengembangan Taman Toga Sebagai Bahan Baku Resep Toga Berbasis Biosaince pada Kelompok Pkk Rw 7 Bangkong Kecamatan Gunungpati Kota Semarang Iswari, Retno Sri; R. Susanti, R. Susanti; Saptono, Sigit; Sasi, Fitri Arum; Laila, Nafisatul
Life Science Vol 10 No 1 (2021): April 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i1.47176

The spread of the COVID-19 virus that has occurred recently is fast the antiviral drugs are not yet available. The spread has to reduce the virus for mild sufferers, self-isolation is requested and for severe sufferers to be isolated in the hospital. One of the efforts to prevent the transmission of COVID-19 is to increase endurance through the use of family medicinal plants (Toga). The potential of medicinal plants as immunomodulators is widely spread in Bangkong village. Bangkong is one of the sub-districts in Semarang City that is also affected by Covid-19, each resident has enough yard to make Toga park. This service activity aims to: (1) provide skills to PKK RW 7 women in designing Toga gardens by making Toga plant racks to decorate their home gardens which can serve as the basic ingredients for making ethnoscience-based Toga recipes; (2) providing knowledge and skills in cultivating Toga; (3) providing knowledge and skills in making ethnoscience-based herbal recipes; (4) providing skills in compiling ethnoscience-based cookbooks about the benefits and ways of presenting them. The approach methods used in the implementation are workshops, lectures/outreach, and training / mentoring. The workshop, given to convey theoretical knowledge about landscape architecture based on land area. In this activity the community service team explained how to make Toga's garden suitable for the land, looking beautiful and free from being damaged by chickens. The results of the training were agreed by the PKK mothers to make Toga in the form of a shelf. It is hoped that the training at the first meeting will produce Toga garden designs that can ensure that each Toga plant can live freely without being disturbed by one another. The lecture method is carried out to explain the benefits of medicinal plants, types of medicinal plants, how to reproduce, and how to treat them. The training method is carried out to provide skills to mothers in making recipes and herbal medicine that can be used to prevent Covid-19 infection. Mothers are very responsive in participating in extension activities on various kinds of plants that can increase endur. After participating in this activity, mothers have understood well the benefits of Toga to increase endurance and prevent being infected with the Corona - 19 viruses Keywords: Bangkong Semarang, Eetnoscience, Taman toga, Bangkong Semarang, Eetnoscience, Taman toga Penyebaran virus covid-19 yang terjadi akhir-akhir ini sangat cepat, obat antivirus belum tersedia. Untuk mengurangi penyebaran virus bagi penderita ringan diminta isolasi diri dan bagi penderita berat diisolasi di rumah sakit. Salah satu upaya pencegahan penularan covid-19 adalah dengan meningkatkan daya tahan tubuh melalui pemanfaatan tanaman obat keluarga (Toga). Potensi tanaman obat sebagai imunomodulator sangat banyak tersebar di lingkungan desa Bangkong. Bangkong merupakan salah satu kelurahan di Kota Semarang juga terdampak covid-19, tiap warga mampunyai halaman yang cukup untk membuat taman Toga. Kegiatan pengabdian ini bertujuan untuk: (1) memberikan keterampilan kepada ibu-ibu PKK RW 7 mendesain taman Toga dengan membuat rak tanaman Toga sebagai penghias halaman rumah yang dapat berfungsi sebagai bahan dasar membuat resep Toga berbasis etnoscience; (2) memberikan pengetahuan dan keterampilan dalam membudidayakan Toga; (3) memberikan pengetahuan dan keterampilan dalam membuat membuat resep jamu berbasis etnoscience; (4) memberikan keterampilan menyusun buku resep berbasis etnoscience tentang manfaat dan cara penyajiannya. Metode pendekatan yang digunakan dalam pelaksanaan adalah workshop, ceramah/penyuluhan, dan pelatihan/pembimbingan. Workshop, diberikan untuk menyampaikan pengetahuan teoritis tentang arsitektur lanskap berdasarkan luas lahan. Pada kegiatan ini team pengabdian menjelaskan bagaimana membuat taman Toga yang sesuai dengan lahan, terlihat indah dan terbebas dirusak oleh ayam. Hasil pelatihan disepakati para ibu PKK membuat Toga dalam bentuk Rak. Pelatihan di pertemuan pertama diharapkan dihasilkan disain taman Toga yang dapat menjamin masing-masing tanaman Toga bisa hidup bebas tidak saling terganggu. Metode ceramah dilakukan untuk memberikan penjelasan tentang manfaat tanaman obat, jenis-jenis tanaman obat, cara memperbanyak dan cara perawatannya. Metode pelatihan dilakukan untuk memberikan keterampilan kepada ibu-ibu dalam membuat resep dan jamu yang dapat digunakan mencegah terkena Covid - 19Setelah mengikuti kegiatan ini, ibu-ibu sudah memahami dengan baik manfaat Toga untuk meningkatkan daya tahan tubuh dan mencegah terinfeksi virus Corona â€“ 19 Keywords: Bangkong Semarang, Eetnoscience, Taman toga, Bangkong Semarang, Eetnoscience, Taman toga

Metagenomic analysis of intestinal microbiota in geese from different farming systems in Gunungpati, Semarang R Susanti; Ari Yuniastuti; Fitri Arum Sasi; Muchamad Dafip
Indonesian Journal of Biotechnology Vol 25, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.53936

The diversity of intestinal bacteria in geese correlates with environmental conditions, rearing methods, and consumed feeds. The intestinal bacteria composition is useful for the absorption of nutrition, improving the metabolism, and may be related to the immune system. This study was conducted to examine the intestinal bacteria composition and the diversity of maintained goose in aviaries and barns. This research was an observational exploratory. Five geese were taken purposively from local breeders in Gunungpati District, Semarang City. A total of 5 g of intestinal contents from each sample was used for microbial genome isolation. Then, the genome was amplified to collect 16S rRNA gene region V3-V4. The amplicons were then sequenced using the next generation sequencing (NGS) method (Illumina high-throughput sequencing; paired-end reads) and analyzed using QIIME2 to identify bacterial species. In addition, GC-MS was performed to identify and measure fatty acid contents in the intestinal. The results showed that both rearing and caged goose contained nine phyla of intestinal bacteria. The number of intestinal bacteria of barn geese (SU) reached 32,748 Operational Taxonomy Units (OTU); higher than aviary geese (SK), which was 11,646 OTU. The intestinal bacteria community in barn geese was approved by Phylum TM7 (Saccharibacteria candidate) (53.18%), followed by Firmicutes (32.51%) and Bacteriodetes (5.42%). Whereas on SK Firmicutes was compiled 49.3 4% of total OTU, TM7 (S. candidate) up to 21.17%, and Actinobacteria up to 15.99 %. The abundance of TM7 may contribute to high 9,12-octadecadienoic acid production, while Firmicutes was related to the high production of oleic acid. Based on these data, the reared geese had a more abundant diversity of bacteria than the caged one.

PENGARUH PEMBERIAN MADU KELENGKENG TERHADAP AKTIVITAS ENZIM SUPEROXIDE DISMUTASE DAN KATALASE PADA TIKUS YANG DIINDUKSI Pb Asetat Ari Yuniastuti; Kamilatussainah Kamilatussainah; Fitri Arum Sasi
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2015): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 6 2015
Publisher : Prosiding SNST Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penelitian ini bertujuan untuk mengetahui aktivitas enzim antioksidan superoksida dismutase (SOD) dan katalase dalam plasma tikus wistar yang diiunduksi Pb asetat dan suplementasi madu kelengkeng. Penelitian eksperimental laboratorik, dengan rancangan The Post Test-Only Group Designdilakukan pada bulan Maret 2015 menggunakan hewan coba tikus putih wistar umur 2-3 bulan dengan berat badan 150-250. Sebanyak 20 ekor tikus diadaptasi dan diberi pakan standar selama 2 minggu. Selanjutnya dibagi secara acak menjadi 4 kelompok, yaitu : kelompok kontrol hanya diberi pakan standar, K(-): kelompok tikus yang diberi perlakuan Pb asetat 10 mg/kgBB, P1 : kelompok tikus yang disuplementasi madu kelengkeng 0,9 ml/200 grBB dan Pb asetat 10 mg/kgBB, P2: kelompok tikus yangdisuplementasi madu kelengkeng 1,8 ml/200 grBB dan Pb asetat 10 mg/kgBB dilakukan selama 14 hari. Setelah akhir perlakuan tikus diambil darahnya melalui plexus retroorbitalis untuk diukur kadar SOD dan CAT. Hasil pemeriksaan dianalisis dengan uji Chi Square dengan batas signifikansi = 0,05.Hasil penelitian menunjukkan bahwa pada tikus kelompok kontrol kadar SOD 141,50μ/ml dan CAT 0,8μ/ml; K(-) kadar SOD 77,71μ/ml dan CAT 0,12μ/ml; P1 kadar SOD 289,82 μ/ml dan CAT 1,21. Sedangkan pada tikus P2,kadar SOD 391,59 dan kadar CAT 3,62. Secara statistik terdapat perbedaan signifikan antar kelompok. Kata kunci: antioksidan, katalase, madu kelengkeng, superoksid dismutase

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