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All Journal Jurnal Sain Veteriner SAINS DAN MATEMATIKA Prosiding Seminar Biologi Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Urology agriTECH Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY Universa Medicina Medical Laboratory Technology Journal Molekul: Jurnal Ilmiah Kimia Oto Rhino Laryngologica Indonesiana INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) Proceeding Biology Education Conference Cermin Dunia Kedokteran
Sofia Mubarika H
Department Of Histology And Cell Biology, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada, Jl. Farmako, Sekip Utara, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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The Relation Between the Expression of latent Membrane Protein-1 Sofia Mubarika H, Soeripto, Murtono C,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 02 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: In vitro studies reveal that LMP1 induces the expression of many factors for invasion and metastasis. Objective: The aims of this study were to investigate the role of LMP1 expression on the status of Nand M that take place either directly or sequentially through its relation with the expression IL4, IL6. IL 10. IFN-gamma. Gran2yme B and with protease MMP2. MMP9. either in the serum or in the tumour tissue. Methods: Forty one 41 EBER-RISH confirmed NPC cases with parallel serum were used for this study. Immunohistochemistry was used to study the expression of proteins in the tissue. The serum level of proteins were determined with ELISA. Statistical analysis used Lisrel 8.50. Results: No correlation between the expression of LMP1 with the status of Nand M of the tumour either directly or that sequentially took place through the expression of the cytokines and MMPs either in the tumour tissue or the serum level. The serum level of MMP9 was correlated with the serum level of IL6, the status of N. and the expression of IL 10. Status N was correlated with serum level of MMP2. Conclusion: There is a process that sequentially took place through the increase of the cytokines. MMPs, either in the tumour tissue or serum level and N. M status. LMP1 expression was not involved in the process. Key words: NPC - metastasis - MMP - cytokine
Gene and antibody molecule Sofia Mubarika Haryana, Sofia Mubarika Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 13, No 02 (1981)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Five major structural types of immunoglobulin in man can be distinguished. i. e. immunoglobulin G (abbreviated to IgG), IgM, IgA, IgD and IgE. Each type of immunoglobulin can synthesize specific antibody according to the inducing antigen. The specificity of immunoglobulin molecule is found in the antigen-binding fragment.Actually the specificity and the diversity of the immunoglobulin is controlled by the gene in the chromosome. The diversity and where the assembly of immunoglobulins is held are described.Key Words: immunoglobulin - antigen-binding fragment - central dogma - protein synthesis - germ line theory
The Effects of new T cell line derived lymphokines on B cell activation Sofia Mubarika Haryana, Sofia Mubarika Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Telah diketahui bahwa untuk aktivasi limfosit B diperlukan faktor pertumbuhan yang disebut limfokin atau sitokin. Banyak bukti menunjukkan bahwa limfosit T yang teraktivasi menghasilkan substansi yang dapat memacu proliferasi atau diferensiasi limfosit B. Dewasa ini telah dapat diidentifikasi berbagai limfokin yang disebut sebagai interleukin (IL) seperti IL-2, IL-3, IL-4, IL-5, IL-6 dan IL-7 yang ikut berperan dalam aktivasi sel B. Namun, mekanisme aktivasi sel B oleh limfokin sampai sekarang masih terus berkembang dan dipelajari. Untuk dapat lebih memahami aktivasi sel B, dalam penelitian ini telah dilakukan pembuatan suatu T cell line, dan telah dikarakterisasi, yakni menunjukkan fenotipe Thyl+, Lyt1+2+, Dalam penelitian ini berbagai aktivator poliklonal lipopolysaccharide (LPS), ConA, dextran sulfat dipakai untuk memacu T cell line menghasilkan limfokin. Aktivitas limfokin pada supernatannya kemudian dianalisis dengan menggunakan blastogenesis assay dengan 3H-thymidine dan hemolytic plaque assay.Hash penelitian menunjukkan bahwa T cell line menghasilkan faktor pertumbuhan yang memacu proliferasi sel B (B cell growth factor II = BCGF II atau IL-5) dan faktor yang memacu sel B untuk berdiferensiasi (B cell differentiating factor = BCDF atau IL- 6) dan menghasilkan igM dan IgG.Key words : blastogenesis assay -- hemolitic plaque assay -- B cell activation -- T cell line -- lymphokines
The effect of mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) leaf etanolic extract on splenic NK 1.1 cells activity Sofia Mubarika Haryana , Sismindari, Muhammad Ghufron Marsetyawan Soesatyo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 03 (2008)
Publisher : Universitas Gadjah Mada

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Background : Mahkota dewa (Pheleria macrocarpa (Scheff, Boerl) is an herbal medicine which has been used since many years as traditional medicine in Indonesia against infections.Objective: The study was aimed to know the effect of mahkota dewa extract on cellular immune response, in particular NK1.1 cell activities.Methods: Forty C57BL/6 mice used in this study were divided into 8 groups. In this study, different doses of etanolic extract of mahkota dewa leaf were used i.e. 1.05 mg, 2.1 mg, and 4.2 mg/20g BW. Groups I, II, and III were treated with 1 mg, 2.1 mg, and 4.2 mg/20 g BW of the extract daily for 30 days. In group IV, V, and VI the extract was given simultaneously during 30 days, then the mice were infected with 104 cfu of Listeria monocytogenes (A TCC-191151 for stimulating their immune responses. Whereas group VII was untreated control group, and group VIII received only Listeria monocytogenes. All mice were then sacrificed 48 hours after the last treatment. Splenocyte NK1.1 cells were collected then cultured with YAC-1 (ATCC:TIB 1601 target cells for killing activity assay, expressing NKG2D, CD122 and IFNy assay. Results: The results showed that an oral administration of the extract significantly increased the killing activity of splenic NK 1.1 cell against the target, Y AC-1 cell. Moreover, the extract promoted the secretion of IFN-y from NK1.1 cells, and also induced expression of both surface molecule NKG2D and CD 122. The strongest effect stimulation was on the dosis of 2.1 mg/20 g BW.Conclusion: The extract had effect to augment splenic NK1.1 cell activities, as indicated by increasing their killing activity, expression of surface molecules and IFN-y production.Key words: pha/eria macrocarpa - splenic NK1.1 cell activity - YAC-1 cell - IFNy - NKG2D and CD122 surface molecules
Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line Astuti, Endang; Sunarminingsih, Retno; Jenie, Umar Anggara; Mubarika, Sofia; S, Sismindari
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Abstract

Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. Ingeneral, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line,the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancerdrugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains somesecondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Basedon these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecularmechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil,immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras proteinwhile TUNEL test was performed to determine the number of apoptosis cells.The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil tomyeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53,caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.
Phalerin, a new benzophenoic glucoside isolated from the methanolic extract of Mahkota Dewa [Phaleria macrocarpa (scheff). Boerl.] leaves Hartati W, Mae Sri; Mubarika, Sofia; Gandjar, Ibnu G; Hamann, Mark T; Rao, KV; Wahyuono, Subagus
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 1, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.086 KB) | DOI: 10.14499/indonesianjpharm0iss0pp51-57

Abstract

Mahkota Dewa [Phaleria macrocarpa (Scheff). Boerl.] is used traditionally to treat cancers in Indonesia. Extract methanol of the leaves displayed a small LC50 value (63.16 μg/ml) on BST (Brine Shrimp Lethality test) assay; therefore a phytochemical study of this extract was undertaken. A new benzophenoic glucoside was isolated from the methanol extract and identified as 4,5-dihydroxy,4’-methoxybenzophenone-3-0-β-D-glucoside (Phalerin) based on its spectroscopic data. Phalerin was cytotoxic to myeloma cell line (NS-1) (in vitro) having IC50 of 83 μg/ml or 1.9 x 10-1 mM.Key words: Phaleria macrocarpa, Phalerin, myeloma
5α-Oleandrin reduce Bcl-2 protein and increase Bax protein expression on Hela cervical cancer cell Wahyuningsih, Mae Sri Hartati; Mubarika, Sofia; Ganjar, Ibnu G.; Wahyuono, Subagus; Takeya, Tatsuo
Universa Medicina Vol 36, No 2 (2017)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2017.v36.102-109

Abstract

BACKGROUNDThe leaves of Nerium indicum Mill. have been utilized traditionally to cure cancer. By using Bioassay guided extraction and isolation method, three compounds (NiO-1, NiO-2, NiO-3) were isolated from an active fraction of the CHCl3 extract of N. indicum leaves. NiO-2 identified as 5α- oleandrin is the best cytotoxic compound on HeLa cervical cancer cell in vitro among the other two. However mechanism of action of the compound hasn’t been evaluated yet. The aims of this study were to determine the mechanism of action at molecular level.METHODSThe action mechanism of 5α-oleandrin on HeLa cervical cancer cells was analyzed by staining the cells with Hoechst 33342; the agarose gel electrophoresis was aimed to determine the DNA fragmentation and the western blotting was aimed to determine the Bcl-2 and Bax protein expression.RESULTSIncubation of HeLa cervical cancer cell with 5α-oleandrin at the concentration 3,47x10-4 mM (24 hours) followed by staining with Hoechst 33342, a broken up light blue color of nucleus was observed (compared with intensive color of untreated control). By gel electrophoresis (at the same concentration of the tested compound), a smear band at about 200 bp was observed. In addition, cells treated with 5α-oleandrin displayed a decreasing of the Bcl-2 protein expression and increasing of the Bax protein expressionCONCLUSION5α-oleandrin induced HeLa cervical cancer cells DNA fragmentation observed by the presence of a smear band at about 200 bp indicative the apoptotic occurrence. 5α-oleandrin induces apoptosis by reducing the Bcl-2 protein expression but the Bax protein expression increases.
Learning Vector Quantization Pada Pengenalan Pola Tandatangan Mubarika, Sofia; Munir, M.; Firdausi, Ketut Sofjan; Widarto, Widarto
JURNAL SAINS DAN MATEMATIKA Volume 14 issue 4 Year 2006
Publisher : JURNAL SAINS DAN MATEMATIKA

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ABSTRACT---In this paper, we analyze and compute distribution of thermal neutron flux in core of Kartini’s Reactor using “Swadaya” detector, both for axial (function of z) and radial (function r) direction. First, “Swadaya” detector is calibrated by activation plate method, which the output from detector is current. The result of calibration is thermal neutron flux (ф) dependent on current (I) that can be written as ф = 4,57x1020I - 2,74x1011. Distribution for neutron thermal flux for axial direction and radial direction respectively Ф = 5,10 x 1012 + 5,99 x 1013 and ф = 1.08 x 1012 + 1,00 x 103 which in reactor core ф has largest value in center position for radial direction.   Keywords: flux neutron distribution, nucleus reactor, Swadaya detector
AKTIVITAS KEMOPREVENSI EKSTRAK TEMU KUNCI (BOESENBERGIA PANDURATA) PADA KARSINOGENESIS KULIT MENCIT BALB/C TERINDUKSI RADIASI ULTRA VIOLET Listyawati, Shanti; Sismindari, Sismindari; Mubarika, Sofia; B. Murti, Yosi
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Temu kunci (B.pandurata) mengandung senyawa-senyawa yang berpotensi antikanker. Penelitian ini bertujuan untuk mengkaji efek kemoprevensi ekstrak etanolik rimpang temu kunci pada karsinogenesis kulit terinduksi ultra violet. Ekstraksi serbuk rimpang B. pandurata dilakukan dengan metode maserasi  menggunakan etanol. Hewan uji yang digunakan adalah mencit Balb/C betina umur 28 hari yang dicukur rambut punggungnya, dikelompokkan menjadi 5 kelompok perlakuan, yaitu kelompok kontrol dan empat  kelompok   perlakuan ekstrak etanolik temu kunci yang diberikan secara oral. Induksi karsinogenesis dengan paparan UV dosis 0,167 J/cm2/hari, sebanyak 60 paparan (5 kali paparan/minggu). Efek penghambatan karsinogenesis kulit  dipelajari pada tingkat insidensi dan multiplisitas kanker. Hasil uji menunjukkan bahwa ekstrak etanolik temu kunci mampu menurunkan angka insidensi dan multiplisitas kanker kulit pada mencit Balb/C terinduksi UV. Ekstrak tersebut berpotensi   dikembangkan sebagai agen kemoprevensi kanker.   Kata Kunci: Boesenbergia pandurata, kemoprevensi, karsinogenesis , ultra violet,
Pengaruh Media Terkondisi Sel Punca Mesensimal (MT-SPM) terhadap Histopatologi Pankreas Tikus Model DM Tipe 2 Bernadia Branitamahisi, Nor Sri Inayat,Sofia Mubarika Haryana,Abdurahman Laqif,Ahmad Hamim, Stefani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 3, No 3 (2018): October 2018
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.035 KB) | DOI: 10.24002/biota.v3i3.1900

Abstract

Diabetes mellitus tipe 2 merupakan jenis diabetes yang paling banyak terjadi. Berbagai macam terapi telah digunakan untuk menangani DM (Diabetes Mellitus) tipe 2, namun masih terdapat keterbatasan. Penelitian ini dilakukan untuk mengetahui pengaruh MT-SPM terhadap histopatologi pankreas dan jumlah sel normal Langerhans pada tikus model diabetes melitus tipe 2. Penelitian ini merupakan penelitian eksperimental murni dengan desain Posttest Control Group. Sebanyak 27 ekor tikus Sprague Dawley jantan dibagi menjadi 3 kelompok: kontrol normal (K(-)) yaitu 9 tikus sehat, kontrol diabetes (K(+)) yaitu 9 tikus yang diinduksi DM tipe 2 dengan 60 mg/kg BB STZ (i.p) dan 120 mg/kg BB NA (i.p), dan kelompok perlakuan (P) yaitu 9 tikus yang diinduksi DM tipe 2 dan diberi perlakuan dengan 0,1 ml/200 g BB (i.p) setiap 3 hari selama 10 kali. Pada hari ke-30 setelah perlakuan, tikus dikorbankan dan diambil jaringan pankreasnya untuk diuji histopatologi dengan pewarnaan Hematoksilin-Eosin. Hasil tersebut dianalisis dengan program Image J. Hasil penelitian menunjukkan terjadi perbaikan pada profil histopatologi pankreas dan terdapat peningkatan jumlah sel normal di islet Langerhans pada kelompok perlakuan yang diberi MT-SPM dibandingkan kelompok kontrol positif.
Co-Authors Agnes Murdiati Akira Hosoyama Aris Haryanto Atsushi Yamazoe Bambang Hariwiyanto Bambang Hariwiyanto Bolhuis RLH Camelia Herdini Camelia Herdini Daan Khambri Danarto Danarto Danarto, Raden Dessy Arisanty Didik Setyo Heriyanto Dinna Rakhmina Edy Meiyanto Endang Astuti Endang Astuti Fatma Zuhrotun Nisa Hideaki Nojiri Ibnu G Gandjar, Ibnu G Ibnu G. Ganjar, Ibnu G. Indwiani Astuti Iqmal Tahir Isnatin Miladiyah Jaap Middeldorp Jajah Fachiro JAKA WIDADA Jumina Jumina Ketut Sofjan Firdausi, Ketut Sofjan KV Rao, KV M. Munir Mae Sri Hartati Wahyuningsih Mark T Hamann, Mark T Mary Astuti Mustofa Mustofa Nastiti Wijayanti Nooter K Nur Arfian Oostrum RG Perkasa, DP Rachma Greta Perdana Putri Retno Arianingrum Retno Sunarminingsih Sudibyo Ronny Martien S. Sismindari Sagung Rai Indrasari Salugu Masesadji Sari Eka Pratiwi Shanti Listyawati SHANTI LISTYAWATI Shinta Hartanto Sismindari . Sismindari Sismindari Soenarto Sastrowiyoto Sri Nuryani Wahyuningrum Subagus Wahyuono Sumadi, Firasti A.N Susanna Hutajulu Tatsuo Takeya, Tatsuo Teguh Aryandono Umar Anggara Jenie Umar Anggara Jenie wardana, Tirta Wirsma Arif Harahap Yanwirasti - Yosi B. Murti