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UJI POTENSI BAKTERI ENDOFIT DARI DAGING BUAH SALAK PONDOH (Salacca edulis) DAN SALAK PADANGSIDEMPUAN (Salacca sumatrana) DALAM MENGHAMBAT PERTUMBUHAN Escherichia coli Rizki Amelia Nasution; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12354

Abstract

Salak is a fruit that contains various health benefits, including the flesh of the fruit which contains bioactive compounds. Taking bioactive compounds directly from the flesh of the salak fruit will require quite a lot of biomass. Obtaining these bioactive compounds can be streamlined by using endophytic bacteria. Endophytic bacteria able to produce compounds of  antibacterial. The aimed of this study to examine the antibacterial potential of  endophytic bacteria derived from the flesh of the Pondoh and Padangsidempuan salak fruit against pathogenic bacteria, namely Escherichia coli. Method this studies is a laboratory experiment. A total of 10 isolates consisting of 6 isolates of Pondoh salak and 4 isolates of Padangsidempuan salak used were the results of isolation from salak fruit flesh in previous studies. Based on the results of the study, ten isolates indicate of antimicrobial activity to Escherichia coli. The inhibition zone diameter of highest value was owned by SP4 isolate, which was 1.1 mm. These results indicate that endophytic bacteria from the pulp of Pondoh and Padangsidempuan salak possess the potential to produce antibacterial compounds against gram-negative pathogenic bacteria, namely Escherichia coli. 
Potensi Bakteri Asam Laktat Sebagai Penghasil Eksopolisakarida Dari Dekke Na Niura Anggy Yohanna Nasution; Rasyidah Rasyidah; Ulfayani Mayasari
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 7, No 3 (2022): September 2022
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v7i3.1236

Abstract

Dekke Na Niura is a typical traditional food of Batak Toba tribe which is made from fermented carp. Dekke Na Niura can act as a source of probiotics because it contains good microorganisms, namely Lactic Acid Bacteria (LAB). Several types of LAB are known synthesize exopolysaccharides (EPS), which are polysaccharide polymers considered important for health. Therefore researchers want to know whether dekke na niura can act as functional food evidenced by the presence of LAB and its potential to produce EPS. The presence of bacterial isolate on dekke na niura was detected by dilution series-pour plate method on MRSA (deMan Rogosa and Sharpe Agar) medium, then confirmed as LAB by morphological and biochemical characterization. The isolates believed to be LAB were then tested for their potential to produce EPS. The results showed that in dekke na niura, LAB isolates were found by UB1, UB2 and UB3 and then all of them identified as Lactobacillus. The three isolates showed the potential for EPS production with a weight ranging from 2490-3490 mg/L. This weight is known to be greater than the weight of EPS produced by commercial LAB isolates.Keywords – Carp, Dekke Na Niura, Lactic Acid Bacteria, Exopolisaccharides
UJI POTENSI BAKTERI ENDOFIT DARI DAGING BUAH SALAK PONDOH (Salacca edulis) DAN SALAK PADANGSIDEMPUAN (Salacca sumatrana) DALAM MENGHAMBAT PERTUMBUHAN Escherichia coli Rizki Amelia Nasution; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12354

Abstract

Salak is a fruit that contains various health benefits, including the flesh of the fruit which contains bioactive compounds. Taking bioactive compounds directly from the flesh of the salak fruit will require quite a lot of biomass. Obtaining these bioactive compounds can be streamlined by using endophytic bacteria. Endophytic bacteria able to produce compounds of  antibacterial. The aimed of this study to examine the antibacterial potential of  endophytic bacteria derived from the flesh of the Pondoh and Padangsidempuan salak fruit against pathogenic bacteria, namely Escherichia coli. Method this studies is a laboratory experiment. A total of 10 isolates consisting of 6 isolates of Pondoh salak and 4 isolates of Padangsidempuan salak used were the results of isolation from salak fruit flesh in previous studies. Based on the results of the study, ten isolates indicate of antimicrobial activity to Escherichia coli. The inhibition zone diameter of highest value was owned by SP4 isolate, which was 1.1 mm. These results indicate that endophytic bacteria from the pulp of Pondoh and Padangsidempuan salak possess the potential to produce antibacterial compounds against gram-negative pathogenic bacteria, namely Escherichia coli. 
Uji Antagonis Bakteri Simbion Spons dari Pulau Bakar terhadap Jamur Candida Albicans Rizki Amelia Nasution; Ulfayani Mayasari
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Abstract

Sponge symbiont bacteria are microbes that live in marine sponges and are known to have bioactive compounds with antimicrobial propeties. Sea sponges on Bakar Island are known to contain many types of symbiotic bacteria. This study was conducted to test the ability of the sponge symbiont bacteria to inhibit the growth of the fungus Candida albicans. The study was carried out in 3 stages, with were sample collection, isolation and antifungal activity test using the well diffusion technique. Sponge symbiont bacteria were obtained as many as 16 isolates and were successfully tested for as many as 11 isolates for their antifungal abilities. Of the 11 isolates, based on the characteristics tested, 1 isolate (SPS4F2) was obtained with strong criteria (12.06 mm) and 10 (SPS1, SPS3, SPS5, SPS6, SPS7 SPS8, SPS9, SPS10, SPS11, SPS12, SPS13) others with moderate criteria (±6.00-9.00). The results of the study can be concluded that the sponge symbiont bacteria from Bakar’s Island mostly have the potential as antifungals against Candida albicans and based of characterization including the Bacillus.
Antibacterial Activity of Kasturi Orange (Citrus microcarpa Bunge) to Inhibit Propionibacterium acnes Ulfayani Mayasari; Fanny Novita; Alfi Sapitri; Rizki Amelia Nasution
International Conference on Sciences Development and Technology The 1st ICoSDTech 2021
Publisher : International Conference on Sciences Development and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.845 KB)

Abstract

Kasturi orange (Citrus microcarpa Bunge) is one of the plants that can be used as medicine, for example to treat coughs, itching or body odor removal. The purpose of this study was to determine whether Kasturi oranges were able to inhibit the growth of Propionabacterium acnes bacteria that causes acne. The research method used is experimental. The experiment consisted of 4 treatments and three repetitions. The results showed that the diameters of the inhibition zones obtained by lime juice at concentrations of 25%, 50%, 75%, 100% were 3.86 mm, 7.13 mm, 9 mm, 11.1 mm. Based on the results obtained, it can be concluded that Kasturi orange juice is able to inhibit the growth of the Propionibacterium acnes bacteria.
Application Of Certainty Factor Method To Diagnose Gastroesophageal Reflux Disease (Gerd) Raissa Amanda Putri; Ulfayani Mayasari; La Saufa Yardha; Siti Septia Febiyaula
International Conference on Sciences Development and Technology The 1st ICoSDTech 2021
Publisher : International Conference on Sciences Development and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.779 KB)

Abstract

An expert system is a computer system that analyzes knowledge, reasoning techniques in problem solving and also facts by following the abilities of an expert. In the expert system there are several methods to help solve the problem, one of which is the certainty factor method or certainty factor which will later be used to solve the problem of gastric acid disease. Because using this method can increase the confidence of an expert. This study aims to design an expert system that will be useful for the community because it can diagnose acid reflux disease or GERD. The design of an expert system is intended to facilitate performance when replacing experts by a project that cannot afford to pay more.
Utilization of Lime Peel (Citrus aurantiifolia (Christm.) Swingle) Ethanol Extract for Mouthwash Formulation to Prevent Dental Caries Alfi Sapitri; Eva Diansari Marbun; Vivi Asfianti; Ulfayani Mayasari
International Conference on Sciences Development and Technology The 2nd ICoSDTech 2022
Publisher : International Conference on Sciences Development and Technology

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Abstract

Lime (Citrus aurantiifolia (Christm.) Swingle) is a plant that has medicinal properties. Lime peel contains alkaloids, flavonoids, tannins, steroids/triterpenoids and essential oils. These compounds are known to have antibacterial activity. This study aims to determine whether lime peel extract has antibacterial activity against Streptococcus mutans and antifungal against Candida albicans. So that an effective inhibition zone will be obtained for the manufacture of mouthwash preparations using the agar diffusion method. The research method used is the experimental method. The experiment consisted of 3 repetitions with a concentration of 96% ethanol extract of lime peel 25%, 30%, 35%, 40%, 45%. Lime peel samples were taken purposively from Pasar 1 Rel, Terjun, Kecamatan Medan Marelan, North Sumatra. The results showed that lime peel extract provided an effective inhibition zone diameter at a concentration of 40% and 45% against Streptococcus mutans with a diameter of 14.07 mm and 14.4 mm at a concentration of 40% and 45% against the fungus Candida albicans with a diameter of 14. mm and 14.2 mm in mouthwash preparations of lime peel extract at FI concentration of 40% FII concentration of 45%, giving satisfactory results of inhibition zone diameter which is greater than 14 mm against Streptococcus mutans and Candida albicans. Lime peel can be formulated in the form of mouthwash preparations.
The Utilization of Soybean Aircraft Waste as A Additional Material in The Process of Manufacturing Goat Feed Materials Intan Fatimah Azzahra intan; Zulia Wirdani Putri Nasution; Revina Yani Tarigan; Muhammad Haris; Ulfayani Mayasari
Journal of Development Research Vol. 6 No. 2 (2022): Volume 6, Number 2, November 2022
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Nahdlatul Ulama Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28926/jdr.v6i2.271

Abstract

The processing process in industry does not only produce the main product but also produces residual production which is usually not used anymore and is called waste. Soybean aircraft waste is a of tempe production by 10% of the total amount of soybean aircraft waste. Environmental problems caused by waste and also the supply of imported feed at a fairly high price underlies researchers to make nutrient-rich feed products by utilizing soybean aircraft waste. The Research content contained in soybean aircraft waste in the form of energy is 306.48 kcal/kg, crude protein 14.45%, fat 3.15% and crude fiber 47.01% . The purpose of this study is to produce a produce a product that is expected to be a solution in reducing waste from the production of soybeans as an additive ]in feed processing. The Methods used in this study is a qualitative descriptive study. The Result in a this journal is additional feed product using soybean aircraft waste that provided benefits for increasing appetite, milk production and meat quality in livestock.
Pelatihan Komputer Dasar dalam Meningkatkan Kompetensi Siswa Menggunakan Metode Participatory Action Research (PAR) Raissa Amanda Putri; Andini Nur Bahri; Franindya Purwaningtyas; Ulfayani Mayasari; Laylan Syafina
Jurnal IPTEK Bagi Masyarakat Vol 2 No 3 (2023): April 2023
Publisher : Ali Institute of Research and Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55537/jibm.v2i3.625

Abstract

This Community Service is carried out at Madrasah Aliyah Al-Washliyah which is located at Jl. KL. Yos Sudarso KM. 6 Tg. Mulia District of Medan Deli. The problem with service partners is that currently they do not have adequate computer laboratories, so that students' abilities in using computer applications are still very limited. Meanwhile, the ability to use computers is now a must for every sector of life. This requires a lot of human resources who have the ability to use computers. Based on this problem, community service was created with the theme of basic computer training in improving the competence of Al-Washliyah Madrasah students. This community service aims to improve the competence of Madrasah Al-Washliyah Medan Deli students in using basic computer applications, namely Microsoft Office Word, Excel and Powerpoint
ISOLASI DAN KARAKTERISASI BAKTERI AMILOLITIK DARI SAMPEL AIR PANTAI PARIS TIGARAS Vila Tria Putri; Rasyidah Rasyidah; Ulfayani Mayasari
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 7, No 1 (2023): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v7i1.12880

Abstract

Amylolytic bacteria have great genetic diversity with high enzymatic activity and have the expertise to break down starch into simpler materials and degrade organic substances in water. This study aims to determine the variety of types of amylolytic bacteria obtained and to see how the characterization of bacteria is isolated from the waters of Paris Tigaras Beach. Isolation of amylolytic bacteria from the waters of Paris Tigaras Beach was carried out using the spread plate method taken from the last dilution of 10-5. Isolates ST3 03 and ST3 04 showed amylolytic activity by showing a clear zone of 11.8 mm in isolates ST3 03 and 8.2 mm in isolates ST3 04. Characterization of isolates ST3 03 and ST3 04 was observed including Gram-positive bacteria with rod-shaped cells, that can produce catalase enzymes, ferment three types of sugars, and move (motil). From the results of observations of the morphology of bacterial colonies and cells, and biochemical tests, isolates of amylolytic bacteria ST3 03 and ST3 04 belong to the genus Bacillus.