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All Journal PROSIDING SEMINAR NASIONAL BIOTROPIA - The Southeast Asian Journal of Tropical Biology Pro Food Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pengabdian Pada Masyarakat Indonesian Journal of Chemistry Gorontalo Journal of Public Health Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Majalah Ilmiah UPI YPTK Research Fair Unisri Buletin Pengabdian Prosiding Seminar Nasional Unimus

Stalis Norma Ethica

Universitas Muhammadiyah Semarang

Author-ID : 261108

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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UTILIZATION OF TIO₂ IMPREGNATED ZEOLIT-ZSM-5 TO DECREASE CONCENTRATION OF CR (VI) IN SOLUTION AT pH VARIANCE Siska Nurprihandayani; Stalis Norma Ethica; Ana Hidayati Mukaromah
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: PROCEEDING 1ST INSELIDEA INTERNATIONAL SEMINAR ON EDUCATION AND DEVELOPMENT OF ASIA (INseIDEA)
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1072.343 KB)

Chromium (VI) or Cr (VI) is one of heavy metal ions, which presence in the environment comes from industrial waste water disposal such as metallic coating, leather tanning and paint industry. Cr (VI) ions are toxic as they could cause lung cancer, chronic infection and polyps. An effort to decrease Cr (VI) concentration has been done by using TiO₂ impregnated Zeolite ZSM-5 (TiO₂-ZSM-5) at 1,25% w/v concentration with pH variation within 75-minute of UV exposure time. This study aims to investigate the effect of pH variation after addition of TiO₂- ZSM-5 powder on Cr (VI) solution, in a way to reduce the presence of the toxic ions in its solution. Object of this study was Cr (VI) solution at concentration of 50 mg/L. The evaluation was carried out on Cr (VI) solutions as samples including vontrol after adding Zeolite ZSM-5 alone, TiO₂ alone and TiO₂ impregnated Zeolite ZSM5 powder. Data analysis was carried out statistically using One-way Annova.The results showed that initial Cr (VI) concentration was 49,73 mg/L and the percentages of Cr (VI) decrease at pH 2, 4, 6, 8 and 10 respectively were 37,55; 34,72; 25.88; 18,10; 11.11%. It means that the highest percentage of Cr (VI) concentration decrease was at pH 2. Based on the statistical analysis results (p value 0,000), the most significant effects in the decreaseof concentation of Cr (VI) solution used in this study were shown by the addition of TiO₂-ZSM-5 powder and by addition of H+ ion (causing pH=2) into the used solution. As conclusion, treatment using TiO₂ impregnatedZeolite ZSM-5 at pH 2 is potential to be used as a way to handle water pollution caused by Cr (VI).

ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE BACILLUS AMYLOLIQUEFACIENS IROD2 PADA ONCOM MERAH PASCA FERMENTASI 48 JAM Dwi Pamaya; Sakti Imam Muchlissin; Endang Tri Wahyuni Maharani; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Proteolytic bacteria are the bacteria capable of producing extracellular protease enzymes, namely protein-breaking enzymes that are widely used in many industrial fields. This study aimed to isolate a proteolytic bacterium found on 48-h post-fermented oncom and molecular identification method. The initial isolation and purification process of the colony was carried out using Nutrient Agar medium. Selection of protease enzyme obtained by bacterial isolate was done on Milk Skim Agar medium. Identification process of the isolate was done through amplification of 16S rRNA gene using PCR, sequencing and analysis of gene sequences using BLAST program. From the isolation process a bacterial isolate that has proteolytic by the ability to produce a clear zone of 82.00 on plate. The result of the 16S rRNA gene sequence analysis showed that the proteolytic bacterial isolate obtained in this study had a 98% homology level with 16S ribosomal RNA isolate of Bacillus amyloliquefaciens strain A1142 (Genbank access code: KTT722836.1). Based on the results of the molecular identification, the isolate was identified as Bacillus amyloliquefaciens strain IROD2 (IROD2 = Indonesia Red Oncom Day2). As conclusion, from 48-h post fermented red oncom, a protease producing bacterial strain molecularly identified as Bacillus amyloliquefaciens strain IROD2. Keywords: Moleculary was identified, Proteolitic bacteria, 16S rRNA gene

PROFIL PROTEIN BERBASIS SDS-PAGE PADA ULAT SAGU PENGASAPAN DENGAN DAN TANPA PENGGARAMAN Alisha Triwahyuni; Ana Hidayati Mukaromah; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Sago Larva (Rhynchophorus ferrugineus) is a high source of animal protein typical from Papua. One of weakness sago larva as foodstuff that is easily rot. To avoid decomposition can be done with fumigation and salting preservation. The purpose of this research is looking at the profile of protein fumigation with and without salting in sago larva. The method used is the method of Sodium Dodecyl Sulfat-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Research sample used about 13 sago larva. One of sago larva used as a control without salting and fumigation, 6 of sago larva used time fumigation variation 2,4,and 6 minutes, than 6 of sago larva used time fumigation variation with salting at concentration 10% b/b. The results showed sago larva as a control has 31 protein bands different with band after fumigation with and without salting. Larva have been smoked for 2 minutes had 30 protein bands, for 4 minutes had 35 protein bands and for 6 minutes had 32 protein bands. While larva with salting and fumigation for 2 minutes has 29 protein bands, for 4 minutes has 22 protein bands and 6 minutes has 36 protein bands. These results indicate that amount of bands at sago larva are diverse because of different sample. The length time of fumigation then the higher level of the protein denaturation as marked with small value of molecular weight. Keywords: Fumigation, Salting, Sago Larva, SDS-PAGE

SAMPLING MIKROBIOLOGI LIMBAH BIOMEDIS RUMAH SAKIT DI KOTA SEMARANG JAWA TENGAH Stalis Norma Ethica; Sakti Imam Muchlissin; Ragil Saptaningtyas; Agus Sabdono
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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The present study aimed to propose a technical strategy of sampling for obtaining microbiological samples from liquid biomedical wastes generated by hospitals in Semarang City, Central Java Province, Indonesia. The samples will be used to evaluate bioremediation ability of the hydrolytic bacteria isolated from the waste. It was hoped that the proposed strategy could help to guide researchers about appropriate sampling practices on hospital biomedical wastes from IPAL of Indonesian hospitals. As mandated by the Indonesian law, the handling of hospital liquid waste in Indonesia should follow Permenkes No.1204/2004, while the liquid waste standard should meet the Kepmen LH No.58/1995, reaffirmed by Perda Provinsi Jawa Tengah No.10/2004 about liquid waste standard issued by the Governor of Central Java. Based on formal regulations and safety aspects, it is proposed that the strategy for a proper microbiological sampling practice in terms of hospital liquid biomedical waste should include: (1) Permit letter from hospital where sampling location is; (2) Relevant sampling method based on research purpose (3) Standard, anti-leaked, properly labelled sampling containers(4) Proper sampling equipment based on sampling purpose (5) Adequate sampling transport equipment; (6) The use of standard personal protection equipment (PPE); and (7) Vaccine for the sampling workers (hepatitis B vaccination ismandatory). Keywords: microbiological sampling, hospital biomedical waste, microbial bioremediation, sampling strategy,Indonesian sampling regulation

PROFIL PROTEIN BERBASIS SDS-PAGE PADA ULAT SAGU HASIL PENGERINGAN DENGAN GARAM DAN TANPA GARAM Aufit Fahima; Ana Hidayati Mukaromah; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.873 KB)

Sago larvae is a potential source of animal protein because it is easy to digest, but it has a weakness, which is easy to rot. To avoid decay can be preserved by drying and salting the sago larvae. The purpose of this study was to determine the protein profile of the sago larvae which was dried with salt and without salt based on SDS-PAGE. The research sample used sago larvae with treatment:, 1) heated in oven with salt and 2) without salt, and also 3) salted without drying at 50 °C using an oven for 1 hour. The results of the study were obtained in the control of 26 bands and there were 6 major bands and 20 minor bands. In samples dried at 50°C using an oven for 1 hour without salting has 21 bands and there are 4 major bands and 17 minor bands. In the salted sample concentration of 10% (b/b) for 1 hour it had 24 bands and there were 5 major bands and 19 minor bands. While the samples were dried and salted with a concentration of 10% (b/b) at a temperature of 50°C using an oven for 1 hour had 19 ribbons and there were 3 major bands and 16 minor bands. Based on the results of this study, the preservation of sago larvae by salting of 10% (b/b) is more recomended better than heating by oven for 1 hour at 50°C. In the salting preservation, number of protein bands of sago larvae based on its protein profile decreases less than that in heating preservation . Keywords : Sago larvae, Drying, Salting, Protein Profile, SDS-PAGE.

GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF Alcaligenes javaensis JG3 POTENTIAL AS AN EFFECTIVE BIODEGRADER Stalis Norma Ethica; Oedjijono Oedjijono; Endang Semiarti; Jaka Widada; Tri Joko Raharjo
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Utilization of glycerol by lipase producing bacteria offers great benefits for fat and oil waste degradation and waterwaste treatment. Nevertheless, there have been lack of reports about the availability of non-pathogenic, lipase producing bacteria, which could naturally degrade glycerol produced from the lipolysis process by lipase. This study reported a newly identified species of rhizobacteria, Alcaligenes javaensis JG3, which is not only able to produce high level of lipase, but also able to degrade glycerol molecules. Identification of strain JG3 was carried out using SEM (Scanning Electron Microscope), BD Phoenix 100 Automated Microbiology System and 16S rRNA gene analysis to determine its taxonomy status. The ability of the strain to metabolize glycerol was investigated both genotypically and phenotypically using degenerate PCR and a glycerol minimal medium. Identification test results showed that strain JG3 belongs to genus Alcaligenes, with the closest relationship with A. faecalis and A. aquatilis (96% nucleotide similarity maximum). Degenerate PCR resulted in a 248-bp sequence showing 93% similarity with glpK of Candidatus Sodalis pierantonius SOPE, a key gene involved in glycerol metabolism. In vitro glycerol utilization test result showed that Alcaligenes sp. JG3 was able to grow on glycerol aerobically, but not anaerobically. It is concluded that Alcaligenes sp. JG3 possesses genes coding for glycerol metabolism and this trait is phenotypically expressed, thus making the strain potential to be used as an effective fat and oil biodegrader.

DETECTION OF VIRULENCE GENES phoP AND phoQ IN Salmonella spp. USING IN SILICO POLYMERASE CHAIN REACTION Stalis Norma Ethica; Hayatun Fuad; Nur Hidayah; Sri Sinto Dewi; Aditya Rahman Ernanto; Ayu Rahmawati Sulistyaningtyas; Richard David Silvestre; Sri Darmawati
RESEARCH FAIR UNISRI Vol. 4 No. 1 (2020): RESEARCH FAIR UNISRI
Publisher : Universitas Slamet Riyadi

Detection of Salmonella bacteria based on their virulence genes is among essential steps in the eradication of clinical infection by bacteria. In this study, two pair of primers, PhoPF-PhoPR: 5’- CCGCGCAGGAAAAACTCAAA-3’ and 5’-ATCTGTTCCAGCATCACCGG -3’ as well as PhoQF-PhoQR: 5’-AGAGATGATGCGCGTACTGG-3’ and 5’- CAGACGCCCCATGAGAACAT-3’, had been successfully designed using Primer3Plus to detect the presence of phoP and phoQ genes in Salmonella spp. Using genomic DNA of 44 genomic data of Salmonella spp. as templates, PhoPF-PhoPR could produce 520-bp amplicon, while PhoQF-PhoQR could result in 598-bp amplicon. Results of in silico PCR showed that both pairs of primers PhoPF-PhoPR and PhoQF-PhoQR could detect only Salmonella enterica species, and no Salmonella bongori species could be detected based on phoP and phoQ sequences. Both pairs of PhoPF-PhoPR and PhoQF-PhoQR primers were also able to detect the virulence genes in most of the studied subspecies of Salmonella enterica available in silico database unless Arizona subspecies. As conclusion, based on this in silico study, phoP and phoQ genes appeared to be biomarkers for Salmonella enterica species. Both pairs of primers designed in this study has potential to be used as detection tool to differentiate species Salmonella enterica from Salmonella bongori, and also to distinguish S.enterica subsp. enterica from subsp. Arizonae.Keywords: Gene detection, bacterial virulence, phoP, phoQ, Salmonella spp.

Streptolysin Encoding Genes sagC and sagD as Biomarkers of Fish Pathogen Streptococcus iniae: An In Silico Study Stalis Norma Ethica; Sri Darmawati; Sri Sinto Dewi; Nurrahman Nurrahman; Ayu Rahmawati Sulistyaningtyas
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 1 (2020): May 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Streptococcus iniae has been notorious as a serious tilapia fish pathogen leading to many disease outbreaks in warm water marine aquaculture. An in silico investigation about the potential of virulence genes of S. iniae, sagC and sagD, as biomarkers of the bacterial species, has been carried out. The aim was to determine bacterial biomarkers, which are important to facilitate early rapid diagnosis of S. iniae streptococcal infection in fish and also in humans. First, specific primers were designed from sagC and sagD genes of S. iniae SF1 genomic DNA using Primer3Plus. Next, in silico PCR (Polymerase Chain Reaction) analysis was carried out using the newly designed primers and 117 genomic DNA of streptococci (all species) retrieved from the database. Primers designed from sagC and sagD genes (SagCF: ‘5- TGCTGACCTCCTAAAAGGGC -3’ and SagCR: ‘5- CTATGCGGCGGGTTTAAGGT -3’ as well as SagDF: 5’- GCCAATCCAATCCTGTCATGC -3’ and SagDR: 5’- TGCAGCTTCCATAACCCCTC -3’) could result in a single band of each matching to 558-bp and 590-bp PCR products only from S. iniae. From 116 other streptococcal genomes studied using similar primers have resulted in no amplicon bands. A further check showed that the amplicons were truly part of sagC and sagD genes of S. iniae. These results showed that sagC and sagD genes appeared to be biomarkers of S. iniae, which are potential to be used to facilitate rapid diagnostic of the pathogenic bacterium.

Detection of rtxA Gene as a Biomarker of Seafood-Borne Pathogen Vibrio cholerae using In Silico PCR Assay Stalis Norma Ethica; Nur Hidayati; Hayatun Fuad; Chaerul Arham; Rivana Ariyadi; Ellyka Purwaningrum; Kazi Mohammad Zillur Rahman
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.417

Seafood-borne outbreaks caused by Vibrio cholerae have led to the increased need for food safety risk assessment of marine products. An in silico investigation about the potential of virulence gene of V. cholerae, rtxA, as a DNA biomarker of the toxigenic bacterium has been carried out. The aim of this study was to use the bacterial DNA biomarker sequence as a tool to facilitate early rapid detection of cholera infection. Five specific pairs of primers were designed from the rtxA open reading frame DNA of V. cholerae O1 biovar El Tor str. N16961 genomic DNA using Primer3Plus. Next, in silico Polymerase Chain Reaction (PCR) assay was carried out using the newly designed primers and 25 genomic DNA of vibrio spp. retrieved from the in silico database. One of the five designed pairs of primers, RtxAOF-RtxAOR: ‘5-CGCAAAACAGTTTCAGCCGA-3’ and 5’-AGGTTGGTCTTTTGTGGCCA-3’, could result in single DNA amplicon sized 518 bp only from V. cholerae species. No amplicon bands were produced from 17 other vibrio genomes studied using similar RtxAF-RtxAR primers. A further check showed that the amplicon was indeed part of the rtxA gene of V. cholerae. Based on this in silico study, rtxA gene appeared to be a DNA biomarker of V. cholerae, which is potential to facilitate rapid diagnosis of the virulence bacterium using in silico PCR assay.

Socialization of the Benefits of Fermenting Cattle Milk into Yogurt as a Probiotic Food Product for Housewife Community of Sruni Village, Musuk, Boyolali Sri Sinto Dewi; Stalis Norma Ethica; Wikanastri Hersoelistyorini
Jurnal Pengabdian Pada Masyarakat Vol 4 No 4 (2019)
Publisher : Universitas Mathla'ul Anwar Banten

Boyolali Regency is among districts in Indonesia, which still has poverty issues and receives direct cash assistance from the government. Yet, villages of the regency including Sruni at Musuk sub-district has been known as one of the main producers of fresh cow milk for the Central Java region.There has been no attempt to process fresh milk into food products of higher economic value at Sruni Village. Meanwhile, results of the strengths, weaknesses, opportunities, and threats (SWOT) analysis at Musuk showed that the region has the potential to be developed for dairy industry. Therefore, through socialization program, community empowerment should be initiated by socializing benefits of fermenting cattle milk into yogurt as a probiotic food product. The socialization had been carried out for 12 housewives in the village of Sruni through two small-class seminars in April 2019. Evaluation was conducted by comparing the number of correct answers from participants’ answers recorded on questionnaire given prior and after each of both seminars. Percentage of improved answers were presented in histograms and then analyzed. As results, the first seminar produced in average 47.4% improved answers, while the second seminar could generate in average 27.3% improved answers. The results showed that in general, the conducted socialization program was quite successful in improving understanding of Sruni villagers on the benefits of fermenting cattle milk into yogurt as a probiotic food product.

Co-Authors A.A. Ketut Agung Cahyawan W Aditya Rahman Ernanto Aditya Rahman Ernanto Aditya Rahman Ernanto Agus Sabdono Agus Suyanto Ainutajriani Ainutajriani Alisha Triwahyuni Alvira Intar Bella Cahya Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Ana Hidayati Mukaromah Aprianti, Nurannisa Fitria Aufit Fahima Aulia Harun Aulia Meirizka Saputra Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Chaerul Arham Dhea Ayu Lestari Dian Purwo Jatinging Putri Dilin Rahayu Nataningtyas Dwi Pamaya Elly Rustanti Ellyka Purwaningrum Endang Semiarti Endang Semiarti Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Fanda Indriyani Fandhi Adi Wardoyo Feri Feri Fuad, Hayatun Hayatun Fuad Hayatun Fuad Istini Istini JAKA WIDADA Jaka Widada Kazi Mohammad Zillur Rahman L. Hartanto Nugroho Maya Linda Shafira Mudyawati Kamaruddin Muhammad Ardi Afriansyah Muhammad Ardi Afriansyah Mukarromah, Ana Hidayanti Mutia Srikandi Fitria Noverson Lidaya Nur Hidayah Nur Hidayah Nur Hidayati Nurcahyono Nurcahyono Nurrahman Nurrahman Nurrahman Nurrahman Oedjijono Oedjijono Puji Lestari Radna Safitri Ragil Saptaningtyas Ragil Saptaningtyas Richard David Silvestre Rivana Ariyadi Rosyida Azis Rizki Sakti Imam Muchlissin Setia Iriyanto Silvestre, Richard David Siska Nurprihandayani Siti Aminah Sofyan Yosias Beslar Sri Darmawati Sri Darmawati Sri Darmawati Sri Elvira Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi suardi suardi Sukowiyono Sukowiyono Sulistyaningtyas, Ayu Rahmawati Theresia Liyana Sejati Tri Joko Raharjo Vicky Mahendra Nurzhulian Vradea Pramesta Wa Ode Inayatul Wikanastri Hersoelistyorini Wikanastri Hersoelistyorini Yuni Nurkuntari

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