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Journal : Agroekotek View

Potensi Cendawan Endofit dari Bunga Bawang Dayak untuk Menekan Pertumbuhan Ralstonia solanacearum pada Tanaman Tomat Putri Wulan Cahyani; Noor Laili Aziza; Yusriadi Marsuni
Agroekotek View Vol 4, No 1 (2021)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/agtview.v4i1.2857

Abstract

Cultivation of tomato plants (Lycopesicum esculentum Mill.) Is often exposed to plant diseases. One of the diseases that often attacks tomato plants is bacterial wilt disease caused by R. solanacearum. Therefore, it is necessary to have biological control with the application of an antagonistic agent, namely the provision of endophytic fungi from dayak onion flowers. This study aims to determine the types of endophytic fungi in dayak onion flowers and to determine the potential of endophytic fungi in suppressing the growth of R. solanacearum. This research was conducted from February to May 2020, taking samples of dayak onion flowers in the Experimental Field of the Faculty of Agriculture and samples of symptomatic tomato plants on the Karang Anyar Farmer Group's land then continued with isolation, purification, identification, and antagonistic testing at the Production Laboratory of the Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru. The method used in this study was a one-factor completely randomized design (CRD) with nine treatments, namely C1 = endophytic fungi A + R. solanacearum, C2 = endophytic fungi B + R. solanacearum, C3 = endophytic fungi F + R. solanacearum, C4 = endophytic fungi G + R. solanacearum, C5 = endophytic fungi I + R. solanacearum, C6 = endophytic fungi J + R. solanacearum, C7 = endophytic fungi K + R. solanacearum, C8 = fungi endophytic N + R. solanacearum, and C9 = endophytic fungi P + R. solanacearum and repeated three times. This study used a comparison, namely control with three replications, in order to obtain 30 experimental units. The results of this study that endophytic fungi from dayak onion flowers have the potential to suppress the growth of R. solanacearum. Based on the research, there were 17 endophytic fungi from dayak onion flowers with nine endophytic fungi which had the fastest growth rate of radius. Fungi with the genus Colletotrichum sp., Mucor sp., and Papulaspora sp. has the potential to suppress the growth of R. solanacearum with moderate to strong percentage of inhibition.
Eksplorasi dan Identifikasi Mikroba Rhizosfer Bawang Merah (Allium ascalonicum L.) yang Diaplikasi Pestisida Nabati di Lahan Gambut Landasan Ulin Kalimantan Selatan Elvina Royani Maliq; Salamiah Salamiah; Yusriadi Marsuni
Agroekotek View Vol 3, No 2 (2020)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/agtview.v3i2.2171

Abstract

Shallot (Allium ascalonicum L.) is one of horticultural plants which is widely used by humans. Onion growth and development are very affected by factors which become obstacles in the cultivation of onion plants. One of the factors that hinder the cultivation of shallot is disease infection that attacks the plants. The disease is caused by fungi, bacteria, viruses, and various other pathogens. It causes a decrease in crop productivity. One of the efforts to get rid of the diseases which attack shallots is using plant-based pesticides. This study aims to determine the various types and the level of diversity of rhizosphere microbial on onion plantations applied with plant-based pesticides on peatlands in South Kalimantan. This research was carried out in the village of Tegal Arum Landasan Ulin and in the Production Laboratory of Agroecotechnology, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru. This research uses descriptive method by taking samples in the onion planting field which are treated with plant-based pesticides. They are P0: Control Treatment, P1: 2.4 g Dithane M-45 fungicide L-1, P2: 1 ml chirinyu extract L -1, P3: 1 ml Kepayang extract L-1, P4: 1 ml Galam extract L-1. The results of identification in this study showed, there were 32 isolates. There were seven types of fungi and two types of bacteria found in the onion rhizosphere that had been applied with various types of plant-based pesticides and has a moderate level of diversity: plan treated by galam-based pesticides (H ′ = 1.75 ), kepayang-based (H ′ = 1.73), Dithane fungicide M-45 (H ′ = 1.15) and control (H ′ = 1.33), onion treated by chirinyuh - based pesticides (H ′ = 0.99) has low level of diversity due to chirinyuh extract has the potential as an antimicrobial.
Uji Konsentrasi Plant Growth Promoting Rhizobacteria (PGPR) dalam Memacu Perkecambahan Biji Poliembrioni pada Biji Jeruk Siam Banjar Sri Wahyuni; Noor Laili Aziza; Yusriadi Marsuni
Agroekotek View Vol 3, No 1 (2020)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.074 KB) | DOI: 10.20527/agtview.v3i1.1414

Abstract

Plant Growth Promoting Rhizobacteria (PGPR) or Rhizobacteria Plant Growth Boosters (RPPT) are non-pathogenic soil microbes found in plant roots that can increase plant growth and stimulate plant growth, PGPR's role in increasing growth and production, especially plant germination is the ability PGPR in synthesizing growth hormones and can provide protection against pathogens that attack plants so it is very good to be applied to a variety of plants. One of them is the banjar orange which is a typical fruit of South Kalimantan which has polyembryony seeds which are several embryos in one seed. Efforts in developing the productivity of banjar siam citrus cultivation are using PGPR. This study aims to determine the effect of the application of PGPR concentrations in spurring the germination of polyembryony seeds in banjar siam beans and to determine the best PGPR concentrations in stimulating the germination of polyembryony seeds in banjar siam seeds. This study uses a completely randomized design (CRD) consisting of a single factor with six replications and five treatments namely P0 (concentration without PGPR), P1 (PGPR concentration 15ml.1-1), P2 (PGPR concentration 30ml.1-1), P3 (PGPR concentration 45ml.1-1), and P4 (PGPR concentration 60ml.1-1). This research was conducted at the Production Laboratory and Integrated Laboratory of Agroecotechnology Department, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru. The research lasted for two months, starting from April to May 2019. The results showed that the application of PGPR concentrations had an effect on spurring the germination of polyembryony seeds on Banjar siam beans at the age of 7 days and 14 days, but the treatment had no effect on the germination of polyembryony seeds at the age of 21 HST, percentage of germination age 7 HST, 14 HST, and 21 HST, the speed of germination either normally or polyembryony, germination length, number of germination, and root length. The best concentration of PGPR in stimulating polyembryony germination in banjar siam seeds is in the control treatment or P0 (concentration without PGPR) at the age of 7 HST and 14 HST.