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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Indonesian Journal of Biotechnology Widyariset Widyariset Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences

Michael Haryadi Wibowo

Departemen Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Author-ID : 277276

Aerospace Engineering Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Veterinary Other

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Isolasi dan uji kepekaan Escherichia coli O157:H7 Isolat lokal asal feses dapi terhadap berbagai jenis antibiotika I Wayan Suardana; I Nyoman Suarsana; Michael Haryadi Wibowo; Dyah Ayu Widiasih
Jurnal Sain Veteriner Vol 29, No 2 (2011): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Escherichia coli O157:H7 is one of the bacteria infection agents that life threatening.

Aktivitas Antiviral Minyak Atsiri Jahe Merah terhadap Virus Flu Burung (ANTIVIRAL ACTIVITY OF ESSENSIAL OIL RED GINGER ON AVIAN INFLUENZA) Tri Untari; Sitarina Widyarini; Michael Haryadi Wibowo
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The studies have reported that ginger have many activities such as antiemesis, anti-inflammatory,anti-bacterial and anti-parasites. Therefore, this study was conducted to evaluate antiviral effect of essentialred ginger oil againts Avian Influenza (AI) in ovo using hemagglutination test (HA). Avian Influenzaviruses were treated with 0,01%, 0,1% and 1% of essential red ginger oil, and then inoculated in chickenembryonated egg via allantoic sac. Allantoic fluid was harvested using for HA test . Result of this studyshows that application of 1% of essential red ginger oil results in the reduction of titer HA . Interestingly,essential oil shows antiviral activity revealed HA titre 20 whereas the titre HA AI which AI virus treatedwith 0,01% and 0,1% essential red ginger oil, the HA titer was 25. The conclution of this study proved thatessensial oil 1% of the red gingger is the best concentration as antiviral activity .

Tapak Perlekatan Reseptor Virus Flu Burung yang Diisolasi dari Berbagai Unggas Sejak tahun 2003 sampai 2008 (RECEPTOR BINDING SITE OF AVIAN INFLUENZA VIRUS H5N1 ISOLATED FROM VARIOUS POULTRIES SINCE 2003 TO 2008) Michael Haryadi Wibowo; Charles Rangga Tabbu; Widya Asmara; Heru Susetya
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian Influenza (AI) is an infectious disease in poultry, caused by type A of avian influenza virus(AIV), in the family of Orthomyxoviridae. Almost all birds’ species are sensitive to the AI. Beside theability to infect various species of poultry. AIV type A has a wide range of host including all bird species,mammals, dan human. Today some scientists reported that the cases of AI in mammals, including humansare increasing. This condition suggests that the AI virus circulated in the field may have some mutationsin the amino acid determinants responsible receptor binding site (RBS). A research was therefore designedto investigate the molecular level of HA gen fragment responsible for receptor binding site of AIV isolatedfrom various poultry since 2003 to 2008. Molecular characterization was based on the amplification ofreceptor binding site of HA gene by reverse transcriptase polymerase chain reaction (RT-PCR). All RTPCRof HA gene positive products were sequenced to determine the nucleotide composition at the targetedfragment. Sequences yielded were analyzed by program Mega 4.0 versions, including multiple alignment,deductive amino acid prediction, and establishment of phylogenetic tree. The results show that all AIVisolates could be determined of some conserved amino acids residues responsible for RBS which indicatethe binding preference of avian like receptor, sialic acid ? 2, 3 galactose except isolate A/Layer/Jabar/MHW-RBS-02/2008 which could be found a deletion of amino acid at position of 129 dan mutation of 151isoleucine into threonine. Phylogenetic study showed that clustering of AIV did not base on species of birdor geographic origin of AI viruses which were studied.

Isolasi dan Identifikasi Bakteri dari Tinja Orangutan Penderita Gangguan Gastrointestinal (BACTERIAL ISOLATION AND IDENTIFICATION IN FAECES OF ORANGUTAN WITH GASTROINTESTINAL DISTURBANCE) Michael Haryadi Wibowo; Antasiswa Windraningtyas Rosetyadewi; Agustina Dwi Wijayanti; Claudia Mona Airin
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Orangutans are among protected animals by the law. One of orangutans’ main health problems isgastrointestinal disease due to bacterial infection. Microbiological data of causative agent of illness inorangutan still not much reported scientifically. This research aim was to identify causative agent ofbacterial infection on gastrointestinal disorder in orangutan isolated from stool samples. The sampleswere collected from Yayasan Konservasi Alam Yogyakarta and Borneo Orangutan Survival, Semboja,Kalimantan Timur. Fresh fecal samples were collected using sterile swab and put them into a steriletransport media. To achieve pure cultures, bacterial isolation was performed by using plate streaking onselective media. Gram stain was done to confirm the cell uniformity and morphology. Bacterialidentification was performed according to Bergey’s Manual Determinative Bacteriology on some biochemicalcharacters to determine the isolated bacteria. The result showed that three bacteria were identified fromstool samples orangutan from Yayasan Konservasi Alam Yogyakarta, i.e.: Citrobacter amalonaticus,Providensia rustigianii, and Proteus mirabilis. Meanwhile, three bacteria, which were Klebsiella planticola,Enterobanter agglomerans and Escherichia coli, were also identified in samples taken from Borneo orangutan.

Isolasi, Identifikasi, Sifat Fisik, dan Biologi Virus Tetelo yang Diisolasi dari Kasus di Lapangan (ISOLATION, IDENTIFICATION, PHISICAL, AND BIOLOGICAL CHARACTER OF NEWCASTLE DISEASE VIRUS ISOLATED FROM FIELD CASES) Michael Haryadi Wibowo; Tri Untari; Anastasia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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native chicken farm suspected to Newcastle disease (ND) virus infection. Specimens were taken andcollected from the lung was further processed. Suspected materials were inoculated into allantoic sacc inspecific pathogenic free of 10 days embryonating egg chicken. The growth of the virus was determined withthe ability to agglutinate the chicken red blood cells or hemaglutination test. Positive hemaglutinationwas performed with hemaglutinatin inhibition test using specific antibody against ND virus. Method forND virus isolation, propagation and identification were based on the standard procedure of serologicalidentification for ND virus serological identification. 13 out of 34 samples were identified as ND viruses.Observation on the course and time of the virus to kill the chicken embryo could be differentiated intomoderate virus patho-type were 10 isolates and a virulent strains were 3 isolates. Further characterizationbased on the elution time observation indicated 11 isolates were not pathogenic strain and 2 isolates werenot virulent strain. Hemagglutinin stability study revealed that 11 isolates were sensitive being heated at560C for 30 minutes while 2 isolates were resistant. Biological characteristic of ND virus to hemagglutinateon various mammalian red blood cells indicating that most isolates were HA negative. Two isolates wereHA positive with cattle, horse and sheep red blood cell, and one isolate indicated positive HA test by usingsheep red blood cell. Control virus was lentogenic patho-type of La Sota strain showed HA and HI testpositive, elution time was 29 minutes, stability on the hemagglutinin after heating was 2 minutes and HApositive with cattle, horse and sheep red blood cell.

Penerapan Metode Diagnosis Cepat Virus Avian Influenza H5N1 dengan Metode Single Step Multiplex RT-PCR Aris Haryanto; Ratna Ermawati; Medania Purwaningrum; Dini Wahyu Yudianingtyas; Michael Haryadi Wibowo; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian influenza (AI) virus is a segmented single stranded (ss) RNA virus with negative polarity andbelong to the Orthomyxoviridae family. Diagnose of AI virus can be performed using conventional methodsbut it has low sensitivity and specificity. The objective of the research was to apply rapid, precise, andaccurate diagnostic method for AI virus and also to determine its type and subtype based on the SingleStep Multiplex Reverse Transcriptase-Polymerase Chain Reaction targeting M, H5, and N1 genes. In thismethod M, H5 and NI genes were simultaneously amplified in one PCR tube. The steps of this researchconsist of collecting viral RNAs from 10 different AI samples originated from Maros Disease InvestigationCenter during 2007. DNA Amplification was conducted by Simplex RT-PCR using M primer set. Then, bysingle step multiplex RT-PCR were conducted simultaneously using M, H5 and N1 primers set. The RTPCRproducts were then separated on 1.5% agarose gel, stained by ethidum bromide and visualized underUV transilluminator. Results showed that 8 of 10 RNA virus samples could be amplified by Simplex RTPCRfor M gene which generating a DNA fragment of 276 bp. Amplification using multiplex RT-PCRmethod showed two of 10 samples were AI positive using multiplex RT-PCR, three DNA fragments weregenerated consisting of 276 bp for M gene, 189 bp for H5 gene, and 131 bp for N1. In this study, rapid andeffective diagnosis method for AI virus can be conducted by using simultaneous Single Step Multiplex RTPCR.By this technique type and subtype of AI virus, can also be determined, especially H5N1.

PATHOGENICITY STUDY OF ESCHERICHIA COLI ISOLATED FROM POULTRY ON BROILER CHICKEN AT 15-DAYS OF AGE Michael Haryadi Wibowo; Agnesia Endang Trihapsari Wahyuni
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was conducted to determine the pathogenicity of Escherichia coli isolated from chickens. Three isolates, Kalasan (Ec/Kls/4/02), Sleman (Ec/Sl/1/02), and Wonosari (Ec/Wno/2/02) were firstly selected and inoculated into broiler chickens at 15 days of age. Two isolates, Kalasan (Ec/Kls/4/02) and Sleman (Ec/Sl/1/02) which showed clear clinical signs and macroscopic lesions, were cultured in Brain Hearth Infussion (BHI) media for 24 hours at 37oC. Serials ten fold dilution of the purified colonies, starting from 10 1 to 10 10 was prepared and 0,5 ml bacterial sample from each dilution were inoculated intraperitoneally into 5 chickens of 15 day-old. The inoculated chickens were monitored for seven days to observe the clinical signs and microscopic lesions. The infective dose (ID)50 of each isolate was determined by Reed and Muench method. The result showed that the two isolates were pathogenic to 15 day-old broiler chickens with specific lesions of pericarditis, perihepatitis, peritonitis, and airsacculitis. Their infective dose-50 was 2 X 10 2,6 bacterial cells per ml for Kalasan isolate and 2 X 10 1,8 bacterial cells per ml for Sleman isolate. It appeared that Kalasan isolate was more pathogenic than Sleman isolate.

Gambaran Mikroskopis Berbagai Organ Merpati yang Menunjukkan Gejala Tortikolis dan Identifikasi Virus Penyakit Tetelo Berdasarkan Uji Agar Gel Presipitasi Risang Aji Dewandaru; Ferdinand Prayogo Cahyo Santoso; Sugiyono Sugiyono; Michael Haryadi Wibowo
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aimed to determine the microscopic conditions of organs of pigeons that suffered from torticollis and identify the cause of disease in torticollis pigeons. Three pigeons showed torticollis symptoms were obtained as sample and marked as pigeon A, B and C, respectively. Isolation of pathogen with inoculation into embryonated pigeon eggs obtained from parents with no history of vaccination and not indicated ND. Identification of ND with agar gel precipitation (AGP) test. Observation of microscopical changes with histopathologic preparation using hematoxylin and eosin staining. Histopathological examination showed that pigeons was done severe neuritis vagus, trakheitis, pneumonia, air sacculitis, hepatitis, pankreatitis, nefritis, jejunoileitis, ileocolitis and orchitis. Perivascular cuffing found in brain. Degenerative changes found in the hepar and ren. Cardiac severe necrotic lesion, and depletion in white pulp area of spleen. Proventricular tissue showed flattening of mucosal epithelium, congestion lesions found in pulmonary tissue. The results of slow hemagglutination test of pigeon egg allantoic fluid, which tested for hemagglutination (HA) showed positive HA result with titers varying between 2, 32, and 64. Serological identification carried out with the AGP test on all culture samples against ND antiserum showing positive results of ND virus. Based on these dara, it can be concluded that the pigeons withsymptoms of torticollis is caused by ND virus.

Analisis Genetik Gen Protective Antigenic pada Bacillus anthracis Isolat Jawa Tengah dan Yogyakarta (GENETIC ANALYSIS ON PROTECTIVE ANTIGENIC GENE OF BACILLUS ANTHRACIS ISOLATES OF CENTRAL JAVA AND YOGYAKARTA) Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of the study was to determine sequence and genotype diversity of protective antigenic gene ofBacillus anthracis isolated from Central Java and Yogyakarta. Pag-A gene which encodes for antigenicprotein is one toxin component and the virulent factor of B. anthracis. As many as five isolates fromSemarang, Sragen, and Boyolali (Central Java) and Sleman (Yogyakarta) were used. The gene wassequenced and amplified used three set of primers PA1857/PA2436, PA8/PA5, and PA-5F/PA-5R. Theresult showed that the nucleotide sequences of gene from five isolates were identical and only had onenucleotide difference as compared to B. anthracis sterne M22589. All isolates were confirmed as genotypebased on pag-A sequence. It was concluded that all B. anthracis from Central Java and Yogyakarta haveidentical pag-A sequence and belong to genotypt-1. Further studies are needed to investigate B. anthracisisolates from other regions of Indonesia.

Resistansi Antibiotik Bakteri dari Ulas Kloaka Burung Puyuh Sehat Maria Anggita; Widya Asmara; Tri Untari; Michael Haryadi Wibowo; Sidna Artanto; Okti Herawati; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Anti Mikrob Resistan (AMR) menjadi masalah utama baik pada manusia, hewan, dan lingkungan. Pada umumnya pemberian antibiotik dilakukan oleh peternak unggas di Indonesia melalui pakan sebagai antibiotik pemacu pertumbuhan/Antibiotic Growth Promoters (AGP). Penggunaan antibiotik yang berlebihan di industri peternakan dianggap berkontribusi terhadap meningkatnya resistansi obat pada manusia. Penelitian ini bertujuan untuk mengetahui kejadian resistansi bakteri dari ulas/swab kloaka burung puyuh sehat. Sebanyak sepuluh ekor puyuh sehat dari peternakan puyuh di daerah Kalasan, Klaten, Yogyakarta diswab kloaka dan dikultur pada media cair Brain Heart Infusion (BHI). Biakan ditanam pada media Mueller Hinton Agar (MHA) dan diletakkan Sembilan jenis cakram/disk antibiotik: streptomisin, doksisiklin, fosfomisin, kloramfenikol, kolistin, siprofloksasin, ampisilin, eritromisin, dan penisilin. Setelah inkubasi pada suhu 37°C selama 18-24 jam, zona hambat yang terbentuk kemudian diukur dan ditentukan sifat resistansi dibandingkan dengan standar. Hasil menunjukkan sebanyak 20%kultur bakteri resistan terhadap streptomisin, 40% resistan terhadap doksisiklin, 40% resistan terhadap kloramfenikol, 50% resistan terhadap kolistin, 20% resistan terhadap siprofloksasin, 20% resistan terhadap ampisilin, 90% resistan terhadap eritromisin, 50% resistan terhadap penisilin, dan tidak ada resistansi terhadap fosfomisin. Terdapat satu dari sepuluh puyuh (P10) yang memiliki resistansi terhadap tujuh dari sembilan jenis antibiotik (78%) yang diujikan, dan dua dari sepuluh puyuh (P2 dan P4) memiliki resistansi terhadap dua dari sembilan antibiotik (11%). Hasil penelitian menunjukkan bahwa bakteri dari swab kloaka pada burung puyuh sehat umur 21 hari dari satu peternakan yang sama memiliki tingkat resistansi yang berbeda-beda. Sifat resistansi terhadap antibiotik dari masing-masing puyuh juga berbeda-beda.

Co-Authors Ade Erma Suryani Aditya Ahkami Pratomo AETH. Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agnesia Endang Trihapsari Wahyuni Agus Eko Srihanto Agus Eko Srihanto, Agus Eko Agustina Dwi Wijayanti Ahmad Sofyan Akbar Agus Anshori Mussama Anastasia Endang Tri Hastuti Wahyuni Antasiswa Windraningtyas Rosetyadewi Arfian Rahma Shanti Arini Nurhandayani Aris Haryanto Bambang Sutrisno Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Charles Rangga Tabbu Claude Mona Airin Devi Yunita Sari Dewi Noor Hidayati Dini Wahyu Yudianingtyas Dito Anggoro Dito Anggoro Dito Anggoro Dyah Ayu Widiasih Eko Agus Srihanto Eko Agus Srihanto Eko Agus Srihanto Ferdinand Prayogo Cahyo Santoso Fidyah Fitrawati Fransiskus Trisakti Haryadi Hendra Herdian Heru Susetya I Nyoman Suarsana I Wayan Suardana I wayan Teguh Wibawan Ifah Khairunnizak Iwan Harjono Utama Khrisdiana Putri Khrisdiana Putri, Khrisdiana Koichi Akiyama Krisdiana Putri Lehgarubini Shanmuganathan Liza Angeliya Liza Angeliya Liza Angeliya Lusty Istiqomah Maria Anggita Marla Anggita Maxs Urias Ebenhaizar Sanam, Maxs Urias Medania Purwaningrum Mohammad Faiz Karimy Niken Respati Maharani Nur Khusni Hidayanto Nyoman Reishita Andriyani Okti Herawati Purnama Edy Santosa Radhian Fadiar Rahmat Setya Adji Ratna Ermawati Risang Aji Dewandaru Sarwo Edy Wibowo Sidna Artanto Sidna Artanto Sidna Artanto Sidna Artanto Sidna Artanto Sitarina Widyarini Siti Andarwarti Sugiyono Sugiyono Surya Amanu Surya Amanu Surya Amanu Surya Amanu Surya Amanu Surya Amanu Syarifah Alawiyah Tri Guntoro Tri Untari Tri Untari Tri Untari Tri Untari Ukon Susetyo Widagdo Sri Nugroho Widya Asmara Widya Asmara Widya Asmara Widya Asmara Widya Asmara Yuli Purwandari Kristiangingrum Yustina Yuni Suranindiyah

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