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PATOGENITAS EDWARSIELLA TARDA PADA IKAN SIDAT (ANGUILLA MARMORATA) SELAMA PENYIMPANAN BEKU -25°C Arsal, Arsal; Hasanuddin, Asriani; Rizal, Ahmad
Mitra Sains Vol 6, No 2 (2018)
Publisher : Tadulako University

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Abstract

The purpose of this study is to determine the patogenechity (ability to cause death) of the Edwarsiella tarda in fish eel (Anguilla marmorata) which have frozen and stored at a temperature of -25°C for 15, 25 and 35 days. Patogenecity was tested with methode by isolating E. tarda from the meat of frosen Eel that what stored for 15, 25 and 35 days ago carried infected to eels the size of 120-122 grams with injecting isolate bacteria Edwarsiella tarda is intraperitoneally as much as 0,1 ml/ind at a dose of 108 CFU/ml (standard 1 Mac Farland), then maintened and observed for 15 days. Pathogenicity test results based on mortality / death of the fish indicates that the bacteria Edwarsiella tarda taken from frozen eel fish stored for 15, 25 and 35 days at temperatures-25oC were still survive and still are pathogenic to fish eel with a tendency the longer the storage time, it will decrease the mortality rate / patogenitasnya. Results of analysis of variance showed mortality observed eel (Anguilla marmorata) with a storage time of 15 days and 25 days resulted in a significantly different effect than the storage time of 35 days with a storage temperature -25oC.
KAJIAN PENGGUNAAN UMBI BUNGA DAHLIA (DAHLIA VARIABILIS) DALAM PRODUKSI KERUPUK IKAN LELE (CLARIAS BATRACHUS) FUNGSIONAL Fajriani, Fajriani; Tantu, Fadly Y.; Hasanuddin, Asriani
Mitra Sains Vol 6, No 1 (2018)
Publisher : Tadulako University

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Abstract

This study examines the use of Dahlia tubers (Dahlia variabilis) in catfish cracers product (Clarias batrachus) as functional food.  This research was conducted in Laboratory of Livestock Technology UNTAD.  The study was conducted on September up to December 2015. Research method that used was AOAC (2009) to examine the content of inulin and proximate test, while for the organoleptic quality used (SNI 01-2346-2006) method. The research design were Completely Randomized Design (CRD) and Random Group Design (RGD). The result of the research showed that the yield generated on processing of Dahlia tubers into flour is 17.73%, the best inulin content contained is 2.14% at the variable F4. The best result of proximate test are at the variable F4 with 7.25% water content, 4.31% ash content, 0.24% crude lipid, and 1.06% crude fiber.  The organoleptic quality of catfish cracers consistenly give unreal influence (p>0.01) toward the appearance , smell , texture and taste while the texture give highly significant effect (p<0.01).
PROFIL ASAM AMINO ISI BURGER DARI KALDU IKAN TONGKOL (EUTHYNNUS AFFINIS) MELALUI PROSES PEMANGGANGAN DAN PENGUKUSAN Finarti, Finarti; Hasanuddin, Asriani; Nasmia, Nasmia
Mitra Sains Vol 6, No 1 (2018)
Publisher : Tadulako University

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Abstract

This study aims to utilize the waste generated from the oil extraction process tuna burger as fill material and to determine the profile of amino acids by product burger filler material from the fish oil extraction process.   The method used in this research was experimental research with t test to test the chemical composition (amino acids, water content, protein content, fat content, and ash content,), treatment with Steam and Bake. Each treatment was repeated two (2) times in order to obtain the number of experimental unit as much as 2 x 2 = 4 experimental units.  The results of the amino acid profile of research in the treatment rose grilled with keseluruan total amino acid in the amount of 15.02% was the steamed at 7.27%. for the amino acid histidine to highest in steam treatment that is equal to 1.41% and 4.74% for the bake treatment, and the lowest is at 0:10 Methionine% at treatment steamed and grilled.  For parameter prosimat highest water content of 43.08% in the treatment steamed while the lowest was in the amount of 24.59% in the treatment of roast. The highest protein content of 10.91% in the treatment roasted while the lowest is equal to 8.81% on a steam treatment. The highest fat content is equal to 1.63% on a steam treatment, while the lowest was 1.51% on a toasted treatment. The ash content of 3.92%, the highest in the treatment of roast and the lowest 2.88% on a steam treatment.
EVALUATION OF ELEUTHERINE (ELEUTHERINE AMERICANA) POTENTIAL AS FEED ADDITIVE FOR POULTRY Rusdi, Rusdi; Hasanuddin, Asriani; Arief, Rosmiaty
AgriSains Vol 17, No 3 (2016)
Publisher : FAPETKAN UNTAD

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Abstract

In vitro studies were exploring eleutherine (Eleutherine americana) potential to be used as feed additive for poultry.  The eleutherine bulbs were extracted using four different organic solvents: methanol, ethyl acetate, diethyl ether and hexane.  The crude extracts were analyzed for their bioactive compounds, tested for anioxidant and antibacterial activity and tested for growth of non-pathogen bacteria. The results indicated that bioactives are indentified:phenol and tannin. Average of antioxidant activity were 33.74, 20.36, 15.14 and 1.98 ppm AEAC  for methanol, ethylacetate, diethylether and hexane, respectively. Furthermore, average of inhibition growth for  pathogen bacteria of Escherichia coli was 5.94, 4.75, 4.56 and 4.44  mm  for  methanol, ethylacetate, diethylether and hexane, respectively, while inhibition for  Staphylococcus aureus was 5.37, 6.50, 5.37 and 5.18  mm for  methanol, ethylacetate, diethylether and hexane, respectively. The extracts also have ability to enhance the gorwth of non-pathogen bacteria Lactobacillus acidophilus. Average of bacteria growth improvement was 21.12, 28.57, 28.16 and 19.59 NTU for methanol, ethylacetate, diethylether and hexane, respectively. The presence of antioxidant and antibacterial activity and growth enhancer activity could be attributed to the bioactive and non digested carbohydrate content of the bulbs extracts.  Therefore, it  could be concluded that the bulbs of eleutherine extract is potential source for “feed additive” as phytobiotics as well as prebiotics.Key words: Eleutherine, Extract, Bioactive, Phytobiotics, Prebiotics 
KAJIAN NILAI NUTRISI LIMBAH PADAT HASIL SAMPING PENGOLAHAN RUMPUT LAUT (Eucheuma cottonii) MELALUI PROSES BIOKONVERSI Hasanuddin, Asriani; Rusdi, Rusdi
AgriSains Vol 13, No 3 (2012)
Publisher : FAPETKAN UNTAD

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Abstract

Government program that sets seaweed and results olahanya as commodity resulted in an increase in the amount of waste from processing into carrageenan seaweed cause environmental pollution. By him that, has done research on the nutritional evaluation of the waste and the efforts to improve the nutritional value of the waste that can be used as feed. This study uses bioconversion technology and using Rhizopus oligosporus as an inoculant. The study was conducted in two phases, namely the early stages of an evaluation study of the nutritional value of waste carrageenan and test its ability to grow on medium Rhizopus oligosporus waste. Furthermore, the implementation of the waste carrageenan bioconversion using Rhizopus oligosporus on some level in the medium (w/w). Preliminary results demonstrate that the solid waste seaweed had higher levels of crude protein 2.43%, fat 0.52%, ash content 4.07%, carbohydrates 7.25%, crude fiber 2.48%, 85.73% water, cellulose 23.29% and hemicellulose 16.51%. Furthermore Showed that Rhizopus oligosporus can grow well on media without the addition of carrageenan waste Nutrients. Bioconversion can improve the nutrient content of solid waste seaweed. Bioconversion of waste produced has a moisture content between 73.91 to 75.97%, protein content between 13.27 to 16.78%, fat content between 0.20 to 0,25%, ash content between 18.15 to 20.60%, a pH value between 6.56 to 7.35 while the crude fiber content Obtained between 13.45 to 20.37%. Based on the series of this study, it can be concluded that the solid waste Eucheuma cottonii seaweed can be used as a growing medium of Rhizopus oligosporus and can improve the nutritional content of seaweed waste. Use of inoculum in the medium waste carrageenan is possible until the level of 15% (w/w).   Key words: Bioconversion, byproducts, seaweed processing, solid waste.
Nutrient Content of Bioconverted Palm Oil Using Rizhopus olygosporus as Feed Marwah, Marwah; Hasanuddin, Asriani; Syahrir, Syahrir
AgriSains Vol 22, No 1 (2021)
Publisher : FAPETKAN UNTAD

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Abstract

The purpose of this study was to determine the nutrient content of the bioconverted palm oil sludge using Rhizopusoligosporus so that it is suitable as functional feed. The used materials were oil palm mud flour and Rhizopusoligosporus fungus. The research design used a factorial completely randomized design with 8 treatments and 3 replications. The treatment consisted of two factors, the first factor was 4 concentrations, namely (0%, 5%, 10% and 15%) and two fermentation times, namely (60 and 96 hours). All treatments were analyzed for nutrient content including dry matter content (BK), crude protein (PK) and crude fiber (SK) fermented palm sludge. The results of the analysis of variety showed that the treatment had a very significant effect (P <0.01) on the content of BK, PK, SK and there was an interaction between the concentration of fungi and fermentation time on SK. The highest dry matter content for BK was obtained in W2K15 treatment (83.24%), PK on W2K15 (18.44%), and SK on W2K15 (24.00%).
The Effect of Concentration Levels Neurospora sp and Times in Fermentation of Palm Oil on Contents of Dry Matter, Crude Protein, and Crude Fiberaw Hasmida, Hasmida; Hasanuddin, Asriani; Syahrir, Syahrir; Aidah, Nur
AgriSains Vol 22, No 2 (2021)
Publisher : FAPETKAN UNTAD

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Abstract

The purpose of this study was to determine the effect of the interaction between the concentration and time of the Neurospora sp. fungus on palm oil fermentation on the dry matter, crude protein and crude fiber content. This study used a completely randomized design (CRD) with a 3x3 factorial pattern, each treatment was repeated 2 times. The first factor was the concentration level of the fungus (0%, 5%, 10%) and the second factor was the fermentation time (48, 72, and 96 hours). The results of the analysis of variance showed that the interaction treatment between the concentration level of Neurospora sp. And the fermentation time of oil palm sludge had a very significant effect (P <0.01) on the dry matter and crude protein content, while the crude fiber content had no significant effect (P> 0.05). ). Independently, the concentration level and time significantly affected the dry matter content, crude protein and crude fiber. The highest value of dry matter was obtained at a combination of 72 hours and a concentration of 10% (W3K10% = 66.323%) and for crude protein, namely 96 hours and a concentration of 10% (W3K10% = 21.430%), while in crude fiber there was a decrease in each time or concentration.
Change Pattern of Mono and Diacylglycerol in Ethanolysis Reaction of Crude Palm Oil Asriani Hasanuddin; . Mappiratu; Gatot Siswo Hutomo
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 3 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The Changging Pattern of Mono and Diacylglycerol concentration as respon to Reaction time during ethanolysis of Crude Palm Oil was influenced by the ration of ethanol/CPO. By using the ration of 0.25-0.5, the changing pattern of MAG concentration followed the parabolic curve, while that of DAG. By using the ration of ethanol/CPO of 1.5, the change of MAG concentration followed polynomyal curve, while that of DAG followed hyperbolic curve. The changing pattern of MAG dan DAG concentration during ethanolysis reaction of crude palm oil may caused by difference of reactivity and position of fatty acid as well as reactivity of lipid group (TAG, DAG, and MAG) Key Word : Ethanolysis, Monoacylglycerol, Diacylglycerol, crude palm oil.
Production and Application of Monoacylglycerol Product from Coconut Oil in the Processing of Coconut Milk . Mappiratu; Dedi Fardiaz; Asriani Hasanuddin
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 3 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Production of Monoglycerol (MAG) from coconut oil was carried out in a reaction system consisting of coconut oil /glycerol/rice bran/hexane in the ratio of 2 : 0.8 : 10 : 40 (w/w/w/v) and agitate at 300 rpm for 90 hours. The rendement was 85.71% and the product contained 49.80% MAG. Fractination with ethanol 95% increade the MAG content. i.e. 86.50%. The fractination rendement was 49.30%. The fraction did not affect the fatty acid of the MAG. Application of fractionated MAG to preserve coconut milk at 0 and 1.0% of MAG did not affect flavor and colour of he coconut milk. In general the appearance of coconut milk containing MAG was better the coconut milk without MAG. Coconut milk containing 0.75% MAG could be stured at cool and room temperature for 12 weeks without microbiological and physical deftect. Key Word : Monoacylglycerol, rendement, application, coconut milk, fractination.
Karakteristik Fisik, Kimia, dan Fungsional Pati Ubi Banggai Asetat pada Berbagai Variasi Waktu Reaksi If&#039;all If&#039;all; Asriani Hasanuddin; Abdul Rahim; Syahraeni Kadir
agriTECH Vol 40, No 4 (2020)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.123 KB) | DOI: 10.22146/agritech.48983

Abstract

Banggai yam plant (endemic in Banggai Kepulauan Regency) is a potential raw material in production of modified starch. The one of techniques to increase the value of starch is modification of starch by acetylation method. This study objectives to determine the physical, chemical and functional characteristics of Banggai acetate yam starch at various reaction times. The study used a Completely Randomized Design with treatment time reaction (T), namely T1: 30 min, T2: 35 min, T3: 40 min, T4: 45 min, T5: 50 min, T6: 55 min. This study was conducted to determine the characteristics of yam acetate starch, which includes percent acetyl, degree of substitution, water and oil holding capacity (WHC/OHC), flammability, solubility, moisture content, ash, fat, protein, starch and amylose starch. The results showed that the longer reaction time under certain conditions will increase the acetyl percent, degree of substitution, WHC, OHC, flareability, solubility but reduce levels of fat, water, ash, fat, protein, starch and amylose starch, yam acetate. In this study at the best reaction time is (50 min), gives the acetyl percent 8.658% and substitution degree of 0.356, the value of WHC, OHC, Swelling ratio and solubility of 32.21%, 30.21%, 1.98 g/g, and 19.17% respectively.