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TAMPILAN REPRODUKSI HASIL INSEMINASI BUATAN MENGGUNAKAN SEMEN BEKU HASIL SEXING PADA SAPI PERSILANGAN ONGOLE DI PETERNAKAN RAKYAT Lieyo Wahyudi; Trinil Susilawati; Nurul Isnaini
TERNAK TROPIKA Journal of Tropical Animal Production Vol 15, No 1 (2014): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.415 KB)

Abstract

The purpose of this research was to determine the success of AI using sexed semen results based on NRR, S/C, CR, and rectal palpation were also determine.  The total number of 81 acceptors divided into 3 treatment groups, such as P0 (non- sexed semen) ; P1 (Frozen semen Sexed using White Yolk Sedimentation),  P2 (Frozen semen sexed using Percoll Gradient Centrifugation) . The results showed the NRR percentage of P0, P1 and P2 were between the treatments are P0 of 74.07 ± 12:00%; 65.43 ± 5.66% and 64.19 ±  8:56% respectively. Furthermore the highest of CR was in P0 44%,   P2 25.91% and P1 18.51%. Conclution of this research was the highest percentage of pregnancy was 59.25 %(P0) ,  P1 51.85% and the lowest was in 44.44%. The S/C was in (P0) 2.31 follow by 3.00 in P2 and 3.33 in P1.   Key words : artificial insemination , frozen semen sexed, the percentage of Gestation and feed
PENGARUH PENGHILANGAN RAFINOSA DALAM PENGENCER TRIS AMINOMETHANE KUNING TELUR TERHADAP KUALITAS SEMEN KAMBING BOER SELAMA SIMPAN DINGIN Abdul Rochim; Muhammad Ade Salim; Nurul Isnaini; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.049 KB) | DOI: 10.21776/ub.jtapro.2017.018.01.5

Abstract

The objective of this research was to examine the effect of removal raffinose in tris aminomethane egg yolk diluents to semen quality of Boer goat during chilled preservation. This research was carried out at Animal Reproduction Laboratory and Sumber Sekar Laboratory, Animal Husbandry Faculty, Brawijaya University from December 11th 2016 to  January 31th 2017. Semen diluent was divided into two groups, there were P0 (80% Tris Aminomethane + 20% Egg Yolk); and P1 (80% Tris Aminomethane (without raffinose) + 20% Egg Yolk). Parameter of this research was motility percentage, viability percentage, and abnormality percentage. Data of this research was analyzed using paired design t test. The result showed that after five days chilled preservation, was different (P<0,05) on the average motility percentage between P0 (39,50±9,26%) and P1 (43,50±6,26%). There was significantly different (P<0,01) on the average viability percentage between P0 (45,86±15,66%) and P1 (46,38±15,32%). There was significantly different (P<0,01) on the average abnormality percentage between P0 (1,76±0,60%) and P1 (2,31±0,75%). Total motile sperm count after 5 days chilled preservation was different (P<0,05) in P0 treatment and not different (P>0,05) in P1 treatment compared to the expectation value of 40 million sperm/ml. The conclution of this research was the tris aminomethane egg yolk diluent without raffinose could protect of Boer goat semen quality during chilled preservation. Keywords: boer goat semen, tris aminomethane, raffinose, chilled preservation
Hubungan Testosteron dengan Motilitas Spermatozoa Tikus Putih Jantan (Ratus Norwegicus ) Setelah Diberi Senyawa Aktif Daun Beluntas untuk Penyusun Buku Antifertilitas Eko Susetyarini; Aloysius Duran Corebima; Moh Amin; Trinil Susilawati
Belantika Pendidikan Vol 2, No 2 (2019)
Publisher : Kayon Media

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.911 KB) | DOI: 10.47213/bp.v2i2.31

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This research helps study the combination of active combination of beluntas leaf (tannin fraction; combined alkaloid + flavonoid + tannin fraction) with various doses of spermatogenic cell counts, spermatozoa quality, testosterone levels, damage to liver cells and black and white skin. This study is an experimental study, using RAK experimental design with a combination of types of active composition of beluntas leaves Based on the results of the study, it can be concluded that there is a combination of active combinations of beluntas leaves (tannin fraction; combined alkaloid + flavonoid + tannin) fractions with various benefits on the number of spermatogenic cells, individual motility, abnormalities, power live in male white rats; there is no combination for spermatozoa concentration and number of motile spermatozoa, and testosterone levels.Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi pemberian jenis senyawa aktif daun beluntas (fraksi tanin; fraksi gabungan alkaloid+flavonoid+tanin) dengan berbagai dosis terhadap jumlah sel spermatogenik, kualitas spermatozoa, kadar testosteron, kerusakan sel hati dan ginjal tikus putih jantan, Penelitian ini merupakan penelitian eksperimen, menggunakan rancangan percobaan RAK dengan kombinasi perlakuan antara jenis senyawa aktif daun beluntas Berdasarkan hasil penelitian, dapat disimpulkan ada pengaruh kombinasi pemberian senyawa aktif daun beluntas (fraksi tanin; fraksi gabungan alkaloid+ flavonoid+tanin) dengan berbagai dosis terhadap jumlah sel spermatogenik, motilitas individu, abnormalitas, daya hidup pada tikus putih jantan; tidak ada pengaruh kombinasi untuk konsentrasi spermatozoa dan jumlah spermatozoa motil, dan kadar testosterone.
TINGKAT KEBERHASILAN INSEMINASI BUATAN DOUBLE DOSIS PADA SAPI PERSILANGAN ONGOLE DENGAN KUALITAS BERAHI YANG BERBEDA Alifian Ibnu Ansori; Kuswati kuswati; Asri Nurul Huda; Rizki Prafitri; Aulia Puspita Anugra Yekti; Trinil Susilawati
REKASATWA : Jurnal Ilmiah Peternakan Vol 3, No 1 (2021): REKASATWA : Jurnal Ilmiah Peternakan
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/rekasatwa.v3i1.11820

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Tujuan dari penelitian ini untuk mengetahui tingkat keberhasilan inseminasi buatan double dosis pada jam ke-8 dan jam ke-16 pada sapi Persilangan Ongole dengan kualitas berahi yang berbeda. Materi dalam penelitian ini menggunakan 25 ekor sapi betina Persilangan Ongole yang dipilih secara purposive. Metode dalam penelitian ini adalah observasi langsung di lapang, dengan menyeleksi sapi betina dengan kriteria BCS >3, umur >1,5 tahun dan telah melahirkan. Penelitian ini menggunakan semen beku pejantan Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Hasil penelitian menujukkan persentase karakteristik yaitu Non Return Rate (NRR-1, NRR-2) masing-masing 92,31% dan 58,33%, Conception Rate (CR) 38,46% dan 25%, Pregnancy Rate (PR) 53,85% dan 50%. Suhu Vagina 37,0-37,9ºC dan >38,0ºC NRR-1, NRR-2 masing-masing 77,78% dan 57,14%, CR 14,28% dan 33,33%, PR 42,86% dan 55,56%. Lendir servik (ada, basah, sedikit) dan (ada, basah, banyak) NRR-1, NRR-2 masing-masing 89,47% dan 16,67%, CR 31,58% dan 16,67%, PR 47,37% dan 66,67%. Lendir Servik pH 7 dan pH 8 NRR-1, NRR-2 masing-masing 77,78% dan 68,75%, CR 11,11% dan 37,50%, PR 44,44% dan 56,25%. Nilai HD 21-30 dan 31-40 NRR-1, NRR-2 masing-masing 50% dan 82,35%, CR 12,50% dan 35,29%, PR 50% dan 52,94%. Kesimpulan penelitian ini bahwa karakteristik warna vulva merah, suhu vagina >38ºC, karakteristik lendir (ada, basah, banyak), lendir servik pH 8 dan nilai HD 31-40, memberikan persentase kebuntingan yang lebih tinggi.
Effect of Body Condition Score Limousine Crossbred Cow on Cattle Oestrus Response Synchronized using PGF2α Single Dose Muhammad Zainul Hanif; Putri Utami; Aulia Puspita Anugra Yekti; Asri Nurul Huda; Joko Riyanto; Chusnul Hanim; Mashudi Mashudi; Trinil Susilawati
Buletin Peternakan Vol 46, No 2 (2022): BULETIN PETERNAKAN VOL. 46 (2) MAY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i2.72976

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One  of  the  strategies  to  increase  the  cattle  population  in  Indonesia  is  the  AI (Artificial   Insemination)  method.  The  AI program's  improvement  of   reproductive efficiency can use the oestrus synchronization method. The purpose of this study was to evaluate   the   effect   of   body   weight   and   body   condition   score   on   the   oestrus synchronization  response with  the brand  "lutelyse."  This  research  was  conducted  in  Senggreng  Village,  Sumberpucung  District,  Malang Regency,  This  research  method uses experimental field experiments  with a sampling technique of purposive sampling. This study took a sample of 21, aged 1,5 –5 years, synchronized with PGF2α as much as  5  ml,  which  was  then  carried  out  by  observing  oestrus  behavior  for  24  hours after synchronization.  The  results  showed  that,  the  percentage  of  oestrus in  cows  with body condition  score  (BCS) 4,  which  is  76.92%,  is  lower  than  cows  with  BCS  5,  which  is 100%.  For  the synchronization  distance  with  the  onset  of  oestrus  in  BCS  4  cattle, namely 3802.92 ± 1007.80 (minutes) and BCS 5, namely 4081 ± 884.91 (minutes) with  the results of the conception rate of BCS 4 cattle of 38.46% and BCS 5 of 50%. Based on the results of the study, it was concluded that BCS 5 has a higher success pregnancy rate than BCS 4.
Effects of mammae hand massages on oxytocin release, milk yield, and milk quality in dairy cows Inggit Kentjonowaty; Achmad Bagus Adhiluhung Mardhotillah; Trinil Susilawati; Puguh Surjowardojo
Livestock and Animal Research Vol 19, No 3 (2021): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.233 KB) | DOI: 10.20961/lar.v19i3.49120

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Objective: The objective of this study was conducted to evaluate the effects of Mammae Hand Massages (MHM) on oxytocin release, milk yield, and milk quality in dairy cows.Methods: Twelve dairy cows with the following criterion: 5-yr-old, 1st to 9th month of lactation, and average body weight of 390 ± 5.55 kg were used. Cows were assigned in a randomized block design with 4 treatments, i.e T0 (without massage), T1 (MHM for 20 s), T2 (MHM for 50 s), and T3 (MHM for 80 s). Oxytocin release, milk yield, and milk quality were measured accordingly.Results: The data obtained were analyzed using analysis of variance (ANOVA). The results showed that MHM had a very significant effect on milk production (P <0.01) as evidenced by the MHM 50 s treatment obtained the highest average milk production (13.08 ± 3.38 liters/head/day) compared to the MHM 20 s, MHM 80 s and control. MHM (1-1.6 volts) for 50 s resulted in the highest release of oxytocin (0.22955 pcg/0.1mL) at 240 seconds compared to control, MHM 20 s and 80 s. Likewise, the percentage of milk protein content (2.96 ± 0.03) and milk fat content (4.27 ± 0.70) was highest at MHM 50 s.Conclusions: It can be concluded that MHM (1-1.6 volts) for 50 seconds increases the release of oxytocin, milk production, and milk quality in terms of the percentage of milk protein and fat content.
Pengaruh pemberian konsentrat pada waktu yang berbeda terhadap keberhasilan inseminasi buatan pada sapi Persilangan Limousin Yadi Malda; Nurul Layla; Auliya Puspita Anugrah Yekti; Asri Nurul Huda; Kusmartono Kusmartono; Trinil Susilawati
Livestock and Animal Research Vol 20, No 3 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v20i3.58662

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Objective: This study aims to evaluate the effect of providing concentrate at different times on the success of artificial insemination in limousin crossbred cow. The material in the study used limousin crossbred cows as many 45 cows, 15 each treatment, cattle between 1,5-6 years old, (BCS) 4-6 (standard 1-9).Methods: This research method uses experimental field trials. Data analyzed used Completely Randomized Block Design (RCBD), grouping based on body weight (BW). Analyzed by ANOVA continued by Duncan Multiple Range Test (DMRT). Treatment T0: field grass + Bio ATP, T1: field grass + concentrate 14 days before oestrus and 7 days after estrus + Bio ATP T2: field grass + concentrate 7 days before oestrus and 7 days after estrus + Bio ATP.Results: The result show that DM consumption T0 (7,74 kg), T1 (12,54 kg) and T2 (12,56 kg), CP consumption T0 (0,62 kg), T1 (0,72 kg) and T2 (0,69 kg), TDN consumption T0 (4,32 kg), T1 (5,23 kg) and T2 (5,17 kg). NRR1 in T0 treatment, T1 and T2 continued 86,67%, 60%, and 73,33%, NRR2 in T0 treatment, T1 and T2 continued 86,67%, 60% and 66,67%. CR in T0 treatment (66,67%), T1 (33,33%) and T2 (26,67%). CR in T0 treatment, T1 and T2 continued 80%, 66,67% dan 40%.Conclusions: The conclusion of this research is that using concentrate has a lower percentage of pregnancy 66,67% and 40% without using concentrate at 80%.
Pengaruh lama thawing dengan air dingin pada semen beku Sapi Peranakan Ongole terhadap kualitas semen Nursahida Ramadhani; Achadiah Rachmawati; Ahmad Budi Purnawan; Trinil Susilawati; Aulia Puspita Anugra Yekti
Livestock and Animal Research Vol 20, No 3 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v20i3.63170

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Objective: The purpose of this research was to determine the effect of thawing durations using cold water on Ongole Crossbred Bull frozen semen towards semen quality including motility, viability, abnormality and total motile spermatozoa.Methods: The method used was experimental in the laboratory using five treatments and ten replications on frozen semen samples of Ongole Crossbred Bull. The data were analyzed using Randomized Block Design variance and continued with DMRT if there was a significant difference. Motility and total motile spermatozoa were tested by Chi Square test to compare with the SNI standard for frozen semen quality. In this research, five treatments were carried out, T0 with a thawing time of 30 seconds with a temperature of 37 oC, while T1, T2, T3 and T4 each used a thawing time of 15, 30, 45 and 60 seconds with temperature of 28 oC.Results: The best thawing results were T2 with individual motility 49.9 ± 6.0 %, viability 65.1 ± 7.2 %, abnormality 4.0 ± 1.8 %, concentration of 39.1 ± 10.2 million/straw and total motile spermatozoa 19.3 ± 4.0 million/straw.Conclusions: In conclusion, the duration and temperature of thawing can affected the quality of Ongole Crossbred frozen semen.
Persentase keberhasilan inseminasi buatan dosis ganda pada sapi persilangan Limousin dengan karakter lendir serviks yang berbeda Nurul Layla; Yadi Malda; Aulia Puspita Anugra Yekti; Asri Nurul Huda; Rizki Prafitri; Kuswati Kuswati; Trinil Susilawati
Livestock and Animal Research Vol 20, No 3 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v20i3.61588

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Objective: The aim of the study was to find out the successful pregnancy in Limousin crossbreed cattle using double-dose AI method on different characters of cervical mucus.Methods: The material used in this study were 45 female cattle with BCS criteria 3-6 on a scale of 1-9 and aged 1.8-6 years. The parameters observed were the condition of the mucus, the pH of the mucus and the electrical resistance of the mucus. AI method uses a double dose method with 4+ semen deposition (cornua utery).Results: The results showed that the highest percentages of NRR 1 and NRR 2 were shown by cattle with the criteria for mucus conditions being present and abundant. It has a pH value of 8 and an electric current resistance is in the range of 20-29 Ω with a percentage of NRR 1 of 62.22%, 53.33% and 71.11%, while the NRR 2 has a percentage of respectively 60.00%, 51.11% and 68.89%. The highest percentage of CR was also shown by cattle with the mucus character, which had percentages of 33.33%, 33.33% and 42.22%, respectively.Conclusions: It was concluded that the highest percentage of pregnancy was shown by cattle with the criteria of mucus being present and abundant, having a pH value of 8 and having an electric current resistance value in the 20-29 Ω category.
Carcass And Wholesalecut Production Of Brahman Cross (BX) Heifer Kuswati Kuswati; Abdul Muhaimin; Wike Andre Septian; Trinil Susilawati
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol. 17 No. 3 (2022)
Publisher : Faculty of Animal Science Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitek.2022.017.03.7

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This research aims to determine the quantitative characteristics of Brahman cross heifer cattle's carcass and wholesale cut at different age levels. The number of heifer cattle taken as samples was 104 heifer. The research method used is a case study with the selection of locations by purpose sampling. Direct observation is used to obtain information on age, slaughter weight, carcass weight, wholesale cut weight, and parts thereof (primary, secondary, and manufacturing cut). The data obtained were then analyzed by analysis of variance (ANOVA). If there was a significant difference, it is followed by a further test of the Least Significant Difference (LSD). The results showed that the age levels in slaughter had a significant effect (P<0.05) on the slaughter weight, fresh carcass weight and wilted carcass weight, but had no effect on the percentage of carcass and wholesale cut. The older the age of cattle and the longer the cattle are raised, the higher their body weight will affect the slaughter weight produced, and the higher the slaughter weight, the higher the carcass weight made. Average slaughter weight at different age by Permanent Incisor (PI): PI0 (<18 months), PI2 (18-24 months) and PI4 (24- 36 months). While the average percentage of heifer carcass at different age levels was relatively the same (52,71% : 53,04% : 53.10%). The weight and rate of wholesale cut and its parts are also somewhat the same. The research results show that the slaughter age affects the slaughter weight and carcass weight but not dressing percentage and wholesale cut weight. Brahman cross heifer cattle at PI4 produced optimal carcass weight and wholesale cut.
Co-Authors Abdul Muhaimin Abdul Rochim Achadiah Rachmawati Achmad Bagus Adhiluhung Mardhotillah Ahmad Budi Purnawan Ali Mahfud Ali mahfud Alifian Ibnu Ansori Aloysius Duran Corebima Annisa Nyuwita Asri Nurul Huda Aulia Puspita Anugra Yekti Aulia Puspita Anugra Yekti Aulia Puspita Anugra Yekti Aulia Puspita Anugra Yekti Aulia Puspita Anugra Yekti Aulia Puspita Anugra Yekti Aulia Puspita Anugra Yekti Aulia Puspita Anugra Yekti Auliya Puspita Anugrah Yekti Ayu Sulvi Istanty Chusnul Hanim Dedi Muhammad Dian Ratnawati Dwi Susan Setiyani Eko Susetyarini Erin Ayu Octaviani Erly Anna Rosita Errico Errico Fatahilah Fatahilah Feri Eka Wahyudi Gatot Ciptadi Hary Nugroho Herni Sudarwati Hirzi Hanifi Ika Nurjannah Inggit Kentjonowaty Irfan Irfan Isnaini Nurul Joko Riyanto Khavida Nuril Muslim Kusmartono Kusmartono Kusmartono Kuswati Kuswati Kuswati Kuswati Lieyo Wahyudi M Ade Salim Mashudi Mashudi Mashudi Moch Nasich Moch Saifudin Moh Amin Moh Thoriq Fernanda Muchamad Lutfhi Mugiyati Mugiyati Muhamad Iqbal Zajulie Muhammad Ade Salim Muhammad Nur Ihsan Muhammad Zainul Hanif Mukhlas Agung Hidayat Nanang Sugiarto Nareswara Hapsari Nisa’us Sholikah Nolasco da costa Nursahida Ramadhani Nurul Isnaini Nurul Isnaini Nurul Isnaini Nurul Isnanini Nurul Layla Nurul Layla Poespitasari Hazanah Ndaru Pramudya Annisa Firdausi Puguh Surjowardojo Putri Utami Rara Putri Audia Ravenska Ravenska Risky Amalia Rosary Rizki Prafitri sri rahayu Sri Wahjuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sudarmanto Sudarmanto Veronica Devita Bunga Wike andre Septian Yadi Malda Yadi Malda Yulianto Nugroho