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All Journal e-Journal Pustaka Kesehatan STOMATOGNATIC- Jurnal Kedokteran Gigi ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Health Notions Cakradonya Dental Journal Odonto dental journal
Melok Aris Wahyukundari
Fakultas Kedokteran Gigi Universitas Jember
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Nursing Public Health

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Journal : Dental Journal (Majalah Kedokteran Gigi)

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The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; P. Purwanto; Pujiana Endah Lestari; Roedy Budirahardjo; Dyah Setyorini; Ristya Widi Endah Yani; Erawati Wulandari; Melok Aris Wahyukundari
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1168.806 KB) | DOI: 10.20473/j.djmkg.v52.i1.p45-50

Abstract

Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.
Co-Authors Achmad Gunadi Amandia Dewi Permana Shita Atik Kurniawati Ayu Mashartini Prihanti Chrysmyta Fiola Viandisa Cici Widya Anggraini Cici Widya Anggraini Depi Praharani Desi Sandra Sari Dian Fajariani Dyah Setyorini Erawati Wulandari Happy Harmono Hestieyonini Hadnyanawati I Dewa Ayu Ratna Dewanti I Dewa Ayu Susilawati Nailah Rahmadani Narita Ajeng Loviana P. Purwanto Peni Pujiastuti Pudji Astuti Pujiana Endah Lestari Ristya Widi Endah Yani Rizka Maya Silvia Roedy Budirahardjo Rosanita Firdausi Oktaviani Sri Lestari Syafira Dwi Astuti Yuliana Mahdiyah Da&#039;at Arina Yuliana Mahdiyah Daat Arina Zakiyya Ulpiyah