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All Journal Biospecies Jurnal Biologi Udayana AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Biologi Universitas Andalas Jurnal Riau Biologia Jurnal Ilmiah Pertanian Jurnal Biodjati Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Bio-Lectura Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Jurnal ABDINUS : Jurnal Pengabdian Nusantara Jurnal KALANDRA

Mayta Novaliza Isda

Jurusan Biologi FMIPA Universitas Riau, Kampus Bina Widya Panam, Pekanbaru, Riau, Indonesia

Author-ID : 288950

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences

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Respons Poliembrioni Dari Biji Duku (Lansium domesticum Corr.) yang Dibelah Tiga Secara In Vitro Syafia Diang Rana; Reza Puspita Dewi; Agung Purnomo Adjie; Mayta Novaliza Isda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 4, No 2 (2019): June 2019
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v4i2.2472

Duku (Lansium domesticum Corr.) adalah salah satu buah tropis bernilai ekonomis yang diminati oleh masyarakat dengan rasa manis dan bernilai gizi tinggi. Biji duku memiliki sifat poliembrioni yang akan menghasilkan tanaman lebih banyak dan seragam sesuai dengan induknya. Perbanyakan dengan teknik in vitro akan menghasilkan tanaman dalam jumlah yang banyak dalam waktu yang relatif singkat dibandingkan secara konvensional. Tujuan penelitian yaitu untuk mengetahui respon poliembrioni biji duku yang dibelah tiga secara membujur dengan penambahan BAP (Benzyl Amino Purine) secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan konsentrasi BAP = 0, 1, 3, 5 dan 7 mg/L BAP. Hasil penelitian menunjukkan bahwa waktu muncul tunas tercepat yaitu 2,20 MST pada konsentrasi 1 mg/L BAP. Jumlah tunas tertinggi pada perlakuan 3 mg/L BAP yaitu 2,00 tunas, namun perlakuan pemberian konsentrasi BAP belum mampu meningkatkan jumlah tunas.

Respons Pembentukan Kalus Daun Pegagan (Centella asiatica (L.) Urb.) dengan Penambahan Naphtalene Acetic Acid dan Benzyl Amino Purin Secara In Vitro Nurhayati Anwar; Mayta Novaliza Isda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 5, No 3 (2020): October 2020
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v5i3.3232

Gotu kola (Centella asiatica (L.) Urb.) is a medicinal plant that contains chemical compounds as triterpenoids and saponins. The chemical compounds can be produced quickly using in vitro callus induction. The callus is a very important source of planting material in regenerating new plants. Therefore, by inducing callus the need for seedling in large quantities achievable in a short time. This study aimed to determine the effect of the BAP single and combination of BAP and NAA, and determine the effective concentration of a BAP single and combination of BAP and NAA on callus induction of gotu kola leaf explant using in vitro method. This study used Complete Randomized Design (CRD) which consisted of nine treatments (control, 1 mg/l BAP, 2 mg/l BAP, 1 mg/l BAP + 0,1 mg/l NAA, 2 mg/l BAP + 0,1 mg/l NAA, 1 mg/l BAP + 0,3 mg/l NAA, 2 mg/l BAP + 0,3 mg/l NAA, 1 mg/l BAP + 0,5 mg/l NAA, 2 mg/l BAP + 0,5 mg/l NAA) with five replication for each treatment. Data obtained from observation were analyzed descriptively. The results of this research showed that a single BAP and combine of BAP and NAA were able provide a response in the form of colour, swelling and callus formation. The best concentration that from the most optimal callus and the highest callus growth (+++) is the treatment of P5 ( 1 mg/l BAP + 0,3 mg/l NAA) which is equal to 100% with the caracteristics of green callus and callus covering the entire surface of explants. All the callus produced was textured compact, while the colour of the callus was green and brown. Keywords: Gotu Kola (Centella asiatica (L.) Urb.), Callus, BAP, NAA Abstrak Pegagan (Centella asiatica (L.) Urb.) merupakan tanaman berkhasiat obat yang mengandung berbagai bahan aktif seperti triterpenoid dan saponin. Bahan aktif tersebut dapat diproduksi dengan cepat menggunakan teknik induksi kalus secara in vitro. Kalus merupakan sumber bahan tanam yang sangat penting dalam meregenerasi tanaman baru. Oleh karena itu, dengan menginduksi kalus kebutuhan bibit dalam jumlah banyak dapat dicapai dengan waktu singkat. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan BAP tunggal dan kombinasi BAP dan NAA, dan menentukan konsentrasi terbaik dari penambahan BAP tunggal dan kombinasi BAP dan NAA terhadap pembentukan kalus dari eksplan daun pegagan secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas 9 (sembilan) perlakuan (kontrol, 1 mg/l BAP, 2 mg/l BAP, 1 mg/l BAP + 0,1 mg/l NAA, 2 mg/l BAP + 0,1 mg/l NAA, 1 mg/l BAP + 0,3 mg/l NAA, 2 mg/l BAP + 0,3 mg/l NAA, 1 mg/l BAP + 0,5 mg/l NAA, 2 mg/l BAP + 0,5 mg/l NAA) dan masing-masing diulang sebanyak 5 (lima) ulangan. Data hasil pengamatan yang diperoleh dibahas secara deskriptif karena tidak semua ulangan menghasilkan kalus. Hasil penelitian menunjukkan bahwa pemberian perlakuan BAP tunggal dan kombinasi penambahan BAP dan NAA mampu memberikan respons pada eksplan daun pegagan berupa perubahan warna, pembengkakan dan terbentuknya kalus. Konsentrasi terbaik yang mampu membentuk kalus paling optimal dan pertumbuhan kalus paling tinggi (+++) yaitu perlakuan P5 ( 1 mg/l BAP + 0,3 mg/l NAA) yakni sebesar 100% dengan ciri-ciri kalus berwarna hijau dan kalus menutupi seluruh permukaan eksplan. Semua kalus yang dihasilkan bertekstur kompak, sedangkan warna kalus yang dihasilkan hijau dan coklat. Katakunci: Pegagan (Centella asiatica (L.) Urb.), Kalus, BAP, NAA

Induksi Tunas dari Eksplan Epikotil Jeruk Kasturi (Citrus microcarpa Bunge.) dengan Penambahan BAP dan Kinetin secara In Vitro Putri Maisarah; Mayta Novaliza Isda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 3 (2021): October 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i3.3416

Jeruk kasturi (Citrus microcarpa) adalah salah satu jenis buah yang termasuk ke dalam genus Citrus, memiliki kandungan vitamin C, dan anti oksidan juga termasuk tinggi. Perkembangan secara generatif setelah berumur 5 tahun. Penelitian ini bertujuan untuk mengetahui efek dan menentukan konsentrasi BAP dan Kinetin terbaik secara tunggal atau kombinasi dari kedua regulator pertumbuhan untuk pembentukan tunas dari eksplan epikotil jeruk kasturi secara in vitro pada media Ms. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) terdiri dari 16 taraf perlakuan (kontrol, 0,5 mg/l BAP, 1 mg/l BAP, 1,5 mg/l BAP, 0,5 mg/l Kinetin, 1 mg/l Kinetin, 1,5 mg/l Kinetin, 0,5 mg/l BAP+ 0,5 mg/l Kinetin, 0,5 mg/l BAP + 1 mg/l Kinetin, 0,5 mg/l BAP + 1,5 mg/l Kinetin, 1 mg/l BAP + 0,5 mg/l Kinetin, 1 mg/l BAP + 1 mg/l Kinetin, 1 mg/l BAP + 1,5 mg/l Kinetin, 1,5 mg/l BAP + 0,5 mg/l Kinetin, 1,5 mg/l BAP + 1 mg/l Kinetin, 1,5 mg/l BAP + 1,5 mg/l Kinetin). Hasil penelitian ini menunjukkan bahwa pemberian perlakuan BAP dan Kinetin tunggal atau kombinasi mampu memberikan respons pada eksplan epikotil jeruk kasturi berupa waktu muncul tunas, jumlah tunas, jumlah daun dan panjang tunas. Konsentrasi terbaik yang mampu membentuk jumlah tunas paling banyak yaitu perlakuan P6 (1,5 mg/l Kinetin) sebesar 1,6 tunas.

Optimasi Konsentrasi Ekstrak Alang-Alang (Imperata cylindrica L.) untuk Memacu Pertumbuhan dan Produksi Jagung Manis (Zea mays Saccharata Sturt) Mayta Novaliza Isda; Wahyu Lestari; Diana Agriani
Al-Kauniyah: Jurnal Biologi Vol 6, No 1 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/al-kauniyah.v6i1.2829

Sweet corn (Zea mays saccharata Struts) a type of corn of high economic value, not only used for consumption also used to make syrup. Imperata is one of the weeds that interfere with crop cultivation, but efforts continue to be made use of weeds as an addition to soil organic matter. The study was conducted to determine the concentration of the extract Imperata (Imperata cylindrica L.) appropriate in the sweet corn growth and determine the concentration of extract proper Imperata in the growth and production. The research method is a Completely Randomized Design (CRD) with 9 treatments and 3 replicates. The results showed that treatment of Imperata extracts with varying concentrations significantly influenced the growth of plant height and leaf number. But not significantly to the growth of the leaf length, leaf width, stem diameter, number of rows per cob, number of seeds per row and plant dry weight. At a concentration of 200 g / 250 ml was able to reduce the vegetative and generative growth of sweet corn.

Induksi Akar pada Eksplan Tunas Anggrek Grammatophylum scriptum var. citrinum secara In Vitro pada Media MS dengan Penambahan NAA Dan BAP Mayta Novaliza Isda; Siti Fatonah
Al-Kauniyah: Jurnal Biologi Vol 7, No 2 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Grammatophyllum scriptum var. citrinum is one variant of endangered from habitat destruction due to logging and forest fires and hunts by orchid collectors. The aim of this study was to determine the effect of BAP and NAA concentration in the root orchid propagation G. scriptum var. citrinum. The results showed that the application of BAP and NAA significant effect on the time of formation appeared roots (19 days), the best root number in the treatment of BAP 0.5 mg/l + 1.0 mg/l NAA for (5 pieces) and root length was obtained NAA 1 mg/l and a combination of 1.0 mg/l BAP + 0.5 mg/l NAA respectively 6.66 cm and 7.40 cm.

Potensi Ekstrak Daun Gulma Babadotan (Ageratum conyzoides L.) terhadap Perkecambahan dan Pertumbuhan Paspalum conjugatum Berg. Mayta Novaliza Isda; Siti Fatonah; Rahmi Fitri
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

The allelopathic substances are an organic agents which produced and released by a plant that cause alteration on the neighboring plants. This research was aimed to determine the effect of Ageratum conyzoides L. leaf extract and also to determine optimum concentration on seed germination and growth of weed Paspalum conjugatum Berg.. This experiment used to extract concentration 0%, 5%, 10%, 15% and 20%. The experiment showed that germination and growth of Paspalum conjugatum Berg.were reduced and to happen damage by leaf extract of Ageratum conyzoides L. The highest germination, growth inhibition and demage at concentrations of the leaf extract 20% respectively are 80.5%, 63.15% dan 17,72 %.

Induksi Kalus dari Eksplan Daun In Vitro Keladi Tikus (Typhonium sp.) dengan Perlakuan 2,4-D dan Kinetin Marlina Agustina Sitinjak; Mayta Novaliza Isda; Siti Fatonah
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Rodent tuber (Typhonium sp.) belongs to Araceae that has a potential as medicinal plant. This procedure takes a long periode to produce a large quantity of plant. Therefore, an in vitro propagation using callus culture is necessary to solve this problem. This research aimed to determine the best concentration of 2,4-D and kinetin to induce callus of in vitro leaf rodent tuber explants. This research used in vitro leaft explants of rodent tuber with MS medium. The design used is Randomize Group Design with 10 treatment. The results showed that explants could not produce callus, the explants response just a swelling leaf explants. The treatment that could give up to 100% swelling response was 2.4-D 0.5 mg / L dan 1 mg / L and the combination treatment 0.5 mg / L 2.4-D + 0.3 mg /L kinetin, 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1.5 mg / L 2.4-D + 0.5 mg / L kinetin. The treatments that could maintain 66.67% explants growth was the combination 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1 mg /L 2.4-D + 0.5 mg / L kinetin.

EMBRIOGENESIS SOMATIK DARI KALUS MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS DENGAN PEMBERIAN BAP DAN MADU SECARA IN VITRO Tirtha Juliana; Mayta Novaliza Isda; Dyah Iriani
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v12i1.5667

AbstrakGarcinia mangostana L. dikenal dengan sebutan queen of the tropical fruits. Buah manggis terbentuk secara apomiksis yang bersifat rekalsitran. Salah satu cara perbanyakan tanaman manggis adalah dengan teknik kultur in vitro melalui embriogenesis somatik. Embriogenesis somatik manggis dilakukan dengan pembentukan kalus terlebih dahulu. Penelitian ini bertujuan untuk menentukan konsentrasi terbaik BAP dan madu secara tunggal serta kombinasinya dalam pembentukan embriogenesis somatik pada kalus biji manggis asal Bengkalis. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan pemberian konsentrasi BAP (3 dan 7 mg/L) dan madu (3, 6, dan 9 mL/L), secara baik tunggal maupun kombinasi, pada media Murashige-Skoog (MS) dengan 3 ulangan. Hasil penelitian menunjukkan bahwa pemberian BAP dan madu dalam seluruh perlakuan tersebut berpengaruh terhadap pembentukan fase-fase embriogenesis somatik kalus manggis. Konsentrasi terbaik dalam pembentukan fase embriogenesis somatik diperoleh dari perlakuan 3 mg/L BAP + 9 mL/L madu dengan presentase pembentukan kalus 100%, waktu muncul kalus 10,67 hst, volume kalus 1,33 dan adanya fase embriogenesis somatik berupa globular, hati, dan torpedo.Abstract Garcinia mangostana L. was known as the queen of the tropical fruits. Mangosteen was formed by apomixis which is recalcitrant. One of the methods of mangosteen propagation is by using a tissue culture technique through somatic embryogenesis. Mangosteen somatic embryogenesis occurs preceded by callus formation. This study aimed to determine the best concentration of BAP and honey in single as well as in combination for the formation phase of somatic embryogenesis in the callus of mangosteen from Bengkalis. The study used a randomized block design with the addition of BAP (3 and 7 mg/L) and honey (3; 6; and 9 mL/L) either single or combination in Murashige-Skoog (MS) medium with 3 replications. The results of this study indicated that the addition of BAP and honey in all treatments affected the phases of somatic embryogenesis of mangosteen callus. The best concentration in the formation of somatic embryogenesis was obtained from, the treatment of 3 mg/L BAP + 9 mL/L which produced 100% of callus formation, with callus emergence time of 10.67 days after plantation, callus volume of 1.33 and the presence of somatic embryogenesis in the form of globular, heart, and torpedo.

Pemberian air rebusan kentang pada media Murashige-Skoog terhadap pertumbuhan protokorm anggrek sendu (Grammatophyllum stapeliiflorum) secara in vitro Fitria Elysye Ningrum; Mayta Novaliza Isda
Jurnal Ilmiah Pertanian Vol. 19 No. 1 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i1.8514

Grammatophyllum stapeliiflorum is one of the most popular orchid species because of its beauty and is characterized by the shape of the flower stalk that sticks down and the brown flowers with white and yellow spots that look like drops of water or sad. This type of orchid is almost extinct and is rarely found in its natural habitat, so it is necessary to perform in vitro propagation of orchids. The culture in vitro method is one of the most effective methods for propagating rare orchids. This study aimed to determine the effect of adding potato water on protocorm growth and determine the best concentration for the growth of G. stapeliiflorum protocorm in vitro. This study used a Completely Randomized Design (CRD) with the addition of potato water (concentrations 0, 50, 100, 150, and 200 ml/L) on Murashige-Skoog (MS) medium. The results showed that the percentage of live explants was 100% in all treatments. The addition of potato water to MS medium had no effect on the percentage of live explants but still affected the growth percentage of Protocorm-like bodies (PLBs) in vitro. The best concentration of potato water for the protocorm growth of G. stapeliiflorum in vitro to MS medium was at a concentration of 150 ml/L, which was indicated by the highest percentage of PLBs growth at 100% and the color of PLBs green (2.5GY 6/8) compared to other potato water treatments and controls.

KAJIAN MORFOLOGI DAN MIKROMORFOLOGI (SISIK SERTA TRIKOMA) 4 JENIS Pyrrosia Mirb. (Polypodiaceae) DI PROVINSI RIAU Nery Sofiyanti; Mayta Novaliza Isda
Jurnal Biologi Tropis Vol. 18 No. 2 (2018): Juli - Desember
Publisher : Universitas Mataram

Pyrrosia merupakanÃÂ golongan tumbuhan paku epifit dari family Polypodiaceae. Penelitian ini bertujuan untuk mengkarakterisasi morfologi (rhizoma, ptiolus dan lamina) dan mikromorfologi (sisik dan trikoma) dari 4 jenis Pyrrosia yang umum dijumpai di Provinsi Riau, yaitu Pyrrosia heterophyla, Pyrrosia lanceolate, Pyrrosia longifolia dan Pyrrosia pillosheloides.ÃÂ Hasil karakterisasi morfologi menunjukan variasi pada organ yang diamati, terutama pada ukuran ptetiolus, bentuk dan ukuran lamina. Hasil pengamatan pada sisik menujukan varias pada warna sisik, jumlah rambut pada sisik dan posisinya. Sedangkan trikoma pada ptiolus dan lamina menunjukan persamaan bentuk yaitu stelata.

Co-Authors Adil Azzahwantara Adjie, Agung Purnomo Afragenia Zelfira Agung Purnomo Adjie Alzira Salsabillah Anwar, Nurhayati Appriliya Destiyana Ariya Permata Asih Rahayu Ajeng Agesti Asri Ria Lestari Ayu Azhari Chica Ollivia Fibrianti Defila Yanti Desita Salbiah DESTI ZARLI MANDARI, DESTI ZARLI Dewi, Reza Puspita Dhini Yulianti Diana Agriani Diandra Prudentia Dyah Iriani EFENDI, RANNY ARIESTA Elli Indriana Putri Elsya Desviyanti Elvianis Elvianis Ennie Chahyadi ERWINA JULIANTARI, ERWINA Fajar Nugraha Febby Ika Desyana Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitmawati Fitria Elysye Ningrum Fransisca Elvarina Hariono, Eko Haris Gunawan Ikhwan Taufik Ilahi, Rizky Nanda Kurnia Ismu Sodaqti Isra Fariza KHAIRIJON, KHAIRIJON Lailani Sabrina Laura Aprilia Ayu Kusuma Wahyu Priyadika M. Athallah Dzikri Alhady M. Rizqy Pratama Syah Marlina Agustina Sitinjak Maulana Ishak Maya Sari Meutia Hanum Meyla Suhendra Mimi Safitri Muhammad Asyraf Muhammad Tamyis Lutfi Hakim Nadia Kristina Najmi Fadhila Nithami Nery Sofiyanti Nia Ivanka Noto Prasetio Nurhafidha Ramadhani Nurhayati Anwar Oriza Athalia Ronald Pangeran Saud Fransisco Putri Maisarah Qintara Sahira Rafi Shadiq Alvi Rahmi Fitri Rana, Syafia Diang Ranny Wirmasari Rasyidah Ulfa Reza Puspita Dewi Riche Afrilla Riski Saputra Rizal Noor Rodesia Mustika Roza Rosmaina Rosmaina Saputra, Agus Sarah Novita Sari Umayah, Sari Savanah Zahra Sinaga, Patar Siti Fatonah Siti Fatonah Siti Fatonah SRI CAHYATI, SRI Surjawati Surjawati Syafia Diang Rana syafroni Pranata Syahrul M. Tirtha Juliana Titrawani Titrawani Vina Dwi Amanda Wahyu Lestari Wahyu Lestari Wulan refolla Yana Lisa Warni Yulia Rahmi

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