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The Adaptation of Small Intestine Nitregic Myenteric Neurons on Rats (Rattus norvegicus) to High Fat Diet Amelia Hana; Sarmin Sarmin; Claude Mona Airin; Christin Marganingsih Santosa; Pudji Astuti
Buletin Peternakan Vol 43, No 2 (2019): BULETIN PETERNAKAN VOL. 43 (2) MAY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i2.38810

Abstract

High fat diet can result in the loss of nitrergic neurons in the myenteric plexus. The study aimed at finding out the effect of high fat diet on the adaptation of nitrergic nerve of rat intestine. It used 15 male rats (Rattus norvegicus) of a month of age with mean body weight of 53.73 gr. The rats were adapted for 7 days to individual cages with ad libitum feeding. After random adaptation, all of the rats were assigned to 3 groups of five rats, namely K-7, K-10, and K-13 groups. Feed and drinking water were given ad libitum. The treatment of the high fat diet lasted for 7 weeks. After the treatment, all of the rats were fasted for 12 hours and then killed. Subsequently, small intestine segments (duodenum, jejunum, and ileum) were taken. The intestinal segments were prepared by using NADPH-d histopathological technique to determine the morphometric changes of nitrergic myenteric neurons. During the treatment the rats were weighed every week and at the end of the study orbitalis vein blood measurement was carried out to see its glucose, cholesterol and cholecystokinin (CCK) plasma levels. The data of body weight, glucose, cholesterol, CCK levels, the total number of the nippergenic myenteric segments of the small intestine were statistically analyzed using Anova. The results of the study showed that the treatment of 7% to 13% fat diets for 7 weeks did not indicate any weight gain and any increase in cholescystokinin level, and any decrease in glucose level. However, it indicated significant increase in cholesterol level. The treatment of 10% and13% fat diets increased the total number of neurons in the jejunum and the ileum. Thus, it was concluded that the treatments of the high-fat diet of the rats (K-7, K-10, and K-13) for 7 weeks had significant effect on the adaptation of the neurons of the jejunum and the ileum.
Refeeding Postmolting Method to Improve Weekly Production Performance of Rejected Laying Hens with Low Mortality Claude Mona Airin; Amelia Hana; Pudji Astuti; Sarmin Sarmin; Trini Susmiati; Okti Herawati
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.45508

Abstract

This research aimed to investigate the influence of gradually feeding rejected laying hens after molting on the performance of production. This research used 6,000 rejected laying hens of 80 weeks old in Subur Farm. Molting method was a modification method by reducing the feed gradually. At the beginning of this research, the feed was given 120 g/laying hens/day and it would be reduced by 10 g/laying hens/day until it reach 50 g/laying hens/day. In the next phase, all chickens fasted for seven days then the chicken was given 10 g/laying hens on the first day. The feed was increased 10 g/laying hens every two days up to 120 g/laying hens/day. Data Collection of Feed conversion ratio (FCR), mortality rate, amount of feed, and egg production were taken at the start of re-feeding. The results showed that during the period of molting until refeeding, mortality was 3.6%, an increase in egg production was seen since week 1, peak of production was 78% at week 9 with FCR 2.3.  The results of statistical analysis showed significant differences (P<0.05) on the percentage of egg production between time periods after the treatment of feed reduction. As the conclusion, molting followed by refeeding in rejected laying hen influences weekly production performance by extending peak production period, optimal FCR and daily egg production.
The Hematological and Blood Chemical Parameters of the Female Fat Tail Sheep Raised with Fermented Complete Feed Management Sarmin Sarmin; Amelia Hana; Pudji Astuti; Claude Mona Airin
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.54669

Abstract

The study aimed at evaluating the hematological and chemical parameters of the blood of female fat tail sheep raised with fermented complete feed (CF) management. It used 40 adult female fat tail sheep age 24-36 months and body weight in 30-35 kg and 3-4 of body condition score, which was raised in the individual stall with fermented complete feed (CF) management. Samples of blood were drawn once from the jugular vein before feeding in the morning. The results of hematological parameters were RBC (12.11±2.18 x106/µL), hemoglobin (12.40±1.96 g/dL), hematocrit  (36.49±5.47%), MCV (30.46±3.38  fL), MCH (10.30±0.75 pg), MCHC (34.00±1.86 g/dL), eosinophils (3.15±7.70%), neutrophils (7.13±14.90%), lymphocytes (11.93±23.85%), and monocytes (0.30±0.88%). The results of chemical parameters were sodium (147.58±3.75 mmol/L), chloride (106.51±3.12 mmol/L), magnesium (3.66±1.38 mg/dL), albumin (3.36±0.31 g/dL) and glucose (40.28±9.12 mg/dL). The results of hematological and chemical parameters in the study could be the reference in monitoring the health of the adult female fat tail sheep raised with the fermented complete feed (CF) management.
Respons Hematologi dan Kimia Darah Domba Lokal Indonesia Terhadap Stres Transportasi Selama 12 Jam (HAEMATOLOGICAL AND BLOOD BIOCHEMICAL RESPONSES TO 12 HOUR TRANSPORTATION STRESS IN IN LOCAL INDONESIAN SHEEP) Sarmin Sarmin; Amelia Hana; Pudji Astuti; Yuda Heru Febrianto; Claude Mona Airin
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.246 KB) | DOI: 10.19087/jveteriner.2019.20.1.48

Abstract

The objective of this study was to investigate the effect of transport 12 hours on haematological and some biochemical responses of local Indonesian sheeps. Ten adults (2.0-2.5 years) male local Indonesian sheeps BW adult 30-40 kg were transported by L-300 pickup truck starting at 3:00 pm to 3:00 am on the d 2 without stopping. Jugular blood were collecte-d at 7 am (-8 h pre- transportation as baseline), 15:00 immadiately after (0 h), 19:00 (4 h), 23:00 (8 h) , 03.00 (12 h ), and at 11.00 d 2 (+8 h) post- transportation as recovery period). Under the conditions of the present study, transport of local Indonesian sheeps significantly increased glucose during 4 h transport (P <0.05), increased eosinophil at 0 h (P <0.05) and no significant changed in erythrocytes, haemoglobin , monocytes, neutrophils, lymphocytes, leukocytes, packed cell volume (PCV), and total plasma protein (TPP) (P> 0.05). Our findings showed that transportation 12 h affected eosinophil during 0 h and glucose metabolism during 4 h of transportation, but did not any affect on some haematology and dehydration responses in local Indonesian sheeps.
Diet Rendah Protein Meningkatkan Jumlah Neuron Nitrergik Duodenum dan Jejunum Serta Menurunkan Bobot Badan Tikus Wistar (LOW PROTEIN DIET INCREASES THE NUMBER OF DUODENUM, JEJUNUM NITRERGIC NEURONS AND REDUCES BODY WEIGHT IN THE WISTAR RATS) Raden Rara Amelia Hana; Sarmin Sarmin; Claude Mona Airin; Pudji Astuti
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.663 KB) | DOI: 10.19087/jveteriner.2018.19.4.539

Abstract

Protein is very important for the growth, development and activity in rats. The impact of lack protein intake to gastrointestinal, especially in the small intestine, the number of nitrergic neurons, and changes in body weight in Wistar rats has not been studied. Therefore the aims of this study to examine the effect of a low-protein diet for 4 weeks on the number of duodenal, jejunal, ilealnitrergicneuron and changes in body weight in Wistar rats. Nine male Wistar (Rattusnorvegicus) rats, aged 1 month, with an average body weight of 120 grams used in this study. The rats were adapted for 7 days in individual cages with a normal diet (containing 24% protein) and drinking ad libitum. After being adapted all rats were divided into 3 groups (K-24, K-14, and K-4), consist of 3 rats. Then all rats fasted for 12-hours. Each group received treatment as follows: K-24 group (as a control) was given a diet containing 24% of protein, K-14 group was given a diet containing 14% of protein, and K-4 group was given a diet containing 4% of protein . Diet and drinking water are given ad libitum. Along of treatment for 4 weeks.body weights and blood glucose were measured every weeks, then all rats were euthanized and sample tissues of duodenum, jejunum, and ileum were then were obtained for histopathological preparations using the NADPH-d technique. Data on the number of nitrergicmyenteric neurons in the duodenum, jejunum, and ileum, body weight gain, and blood glucose levels were statistically analyzed by single factor design Anova. The results of this study concluded that a low protein diet (14% and 4% protein) for 4-weeks in the duodenum, jejunum have been increased the number of nitrergic neurons, on the other hand, in the ileum have been decreased the number of nitrergic neurons, reduced body weight, and did not increase blood glucose levels in Wistar rats.
Pembangunan Infrastruktur Pertanian dan Sosial Dalam Rangka Mempersiapkan Selopamioro Mandiri Sejahtera Berbasis Potensi Lokal Sarmin Sarmin; Irkham Widiyono; Slamet Widiyanto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 1 (2016): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (627.49 KB) | DOI: 10.22146/jpkm.22090

Abstract

Tis program aims to develop agricultural infrastructure and social culture for Selopamioro empowering and prosperous based on local potential. Te priority of  frst year programs were development of water reservoir in Kalidadap 2 and Srunggo, smart home fnishing in Srunggo 1, processed cashew as jam and shredded, promotion assistance in Bantul Expo event, branding and packaging innovation for cashew, to initiate government licensing, and develop standar operating procedure in processed cashew. All of programs as baseline to holistic development in Selopamioro based on local potential.
Pemberdayaan Peternak Marginal: Studi Kasus di Wilayah Banguntapan Bantul Irkham Widiyono; Sarmin Sarmin
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.851 KB) | DOI: 10.22146/jpkm.27031

Abstract

Over the recent years, villagers in the countryside havefaced the problem of limited resources for livestock and crop productions. Decreased land quality and agricultural land areawith respect to the increased need for housing landhas resulted in economic and environmental problems for livestock farmers in this area. Most livestock farmers have poor standard of education, limited ownership of land and livestock, and  are not capable of managing the natural resources in sustainable ways. The present services for small livestock farmersare aimed to transform the unintegrated livestock and agricultural farming into  a sustainable integrated system in the marginal community. Some efforts were conducted to improve knowledge and skills in zero waste system in livestock and agricultural farming by livestock farmers in marginal areas. The present community development programs were conducted using farmer-centered learning methods (participative learning) and mentoring by professionals on the implementation of sustainable integrated farming system (learning by practice). Furthermore, institutional development and cooperative networking with related parties were carried out. Results showed that the livestock farmerswere able to adopt integrated farming practices and the community was capable of independently producing organic fertilizer from livestock wastes and converting agricultural byproducts into animal feed. Both farmers and villagers have come together to cultivate fruit and vegetable plantations in vacant lands and back yards which were previously unused. The farmers’ group has successfully networked with the related partners to ensure sustainability of environmental conservation efforts and improvement of livelihood. In conclusion, active participation of farmersin the marginal area throughout the development process and mentored-practice are key factors for successful agricultural transformation in the marginal community. Implementation of the sustainable integrated farming system will ensure food security, environmental protection and safety, conservation of natural resources, and better quality of life.
Inovasi Tepung Premiks Pancake Mocaf Instan dan Pancake ”Gama Pantelo” di Dusun Gebang, Desa Kemiri, Tanjungsari Kabupaten Gunungkidul Novia Nur Aini; Refika Melina Putri; Sarmin Sarmin; Irkham Widiyono; Soedarmanto Indarjulianto; Wisnu Nurcahyo; Claude Mona Airin; Okid Parama Astirin; Suparni Setyowati Rahayu
Jurnal Pengabdian Pada Masyarakat Vol 5 No 3 (2020)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202053.320

Abstract

INNOVATION OF INSTANT MOCAF PANCAKE PREMIX FLOUR AND PANCAKE OF “GAMA PANTELO” IN GEBANG HAMLET, KEMIRI VILLAGE, TANJUNGSARI GUNUNGKIDUL REGENCY. The purpose of this activity is to empower the Gebang hamlet farmer women's group, Kemiri village, Tanjungsari District Gunungkidul Regency in optimizing mocaf products so that they have time keep the old and not undergo physical and chemical changes, practical and easy to process in the form of Instant Mocaf Flour Pancake Mocaf “Gama pantelo” as a snack or dessert that can be eaten at any time. The method of activity is done by demonstration and hands-on practice. “Gama Pantelo” Instant Premix Pancake Flour is made by pouring mocaf flour, refined sugar, baking powder, milk powder, and vanilli in a basin by passing it on a sieve to get a homogeneous powder size, then stirring it evenly and then mixing the instant mocaf pancake flour in aluminum foil zipperlock packaging. Pancakes are made with instant premix mocaf pancakes mixed with 1 egg and 180 ml of water then stirred evenly using a mixer. Furthermore, after mixing evenly, the dough is cooked on a small teflon that has been previously smeared with a little oil / butter, then baked until a little cavities arise on the surface of the dough and then the dough is turned over and baked until the pancakes turn golden brown. After that it is appointed and can be served. This activity succeeded in arousing the motivation of farmer women's groups in making variations of cassava preparations.
Hormon Progesteron Feses Pada Kambing Peranakan Ettawah Bunting: Progesterone Hormon of Feces on the Pregnant Ettawah Goat Claude Mona Airin; Amelia Hana; Sarmin; Pudji Astuti
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 12 No. 1 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v12i1.224

Abstract

Abstract  The main obstacle of invasive hormone research of Etawah crossbreed goats (PE) were the sample collection due to the aggressiveness of PE goats. Improper ways of handling the goats would result in stress which leads to bias on the data. This research aims to determine the level of fecal progesterone of PE goats during pregnancy. This research use 5 pregnant and 5 non-pregnant PE goats. Diagnosis of pregnancy was conducted by observing the absence of estrus after breeding. Fecal collection was conducted in week 4, 8, 12, 16, 20, and 2 weeks after parturition but sample collection of non-pregnant goats conducted once. The sample of fecal which has been collected then frozen and extracted using methanol 80% as much as 3 ml. The fecal extract was analyzed using EIA KIT progesterone. The results of measurement of fecal progesterone level were 6,282 ± 950.96 ng/gr of feces (week 4 of pregnancy); 18,391.8 ± 2,584 ng/gr (week 8 of pregnancy); 25,958.4 ± 3,447.1 ng/gr of feces (week 12 of pregnancy); 25,233.4 is ± 3,306 ng/gr of feces (week 16 of pregnancy); 18,238.2 ± 3,069.5 ng/gr of feces (week 20 of pregnancy). The level of fecal progesterone in 2 weeks after giving birth was 516 ± 228.16 ng/gr of feces. Meanwhile, the level of fecal progesterone of non-pregnant PE goats is 254.2± 214.5 ng/gr of feces. The conclusion of the progesterone level of PE goats during pregnancy can be detected using the sample of feces with the highest level in week 12 of pregnancy.  Keywords: Feces; Non-invasive; PE Goats; Progesterone   Abstrak  Kendala utama penelitian hormonal secara invasive pada kambing Peranakan Ettawah (PE) pengambilan sampel pada hewan tersebut karena karakteristik kambing PE yang sangat agresif. Handling yang tidak tepat dapat menjadi stresor yang akan menghasilkan data yang bias. Penelitian ini bertujuan untuk mengukur kadar hormon progesteron feses pada kambing PE selama kebuntingan. Penelitian ini menggunakan 5 ekor kambing PE bunting dan 5 kambing PE tidak bunting. Diagnosa kebuntingan dilakukan dengan pengamatan tidak munculnya estrus setelah pengawinan. Pengambilan feses dilakukan pada minggu ke 4, 8, 12, 16 dan ke 20 dan 2 minggu setelah partus. Pada kambing tidak bunting, pengambilan sampel dilakukan 1 kali. Sampel feses yang telah terkumpul, dikeringbeku dan kemudian diekstraksi menggunakan methanol 80% sebanyak 3 ml untuk selanjutnya dianalisis menggunakan KIT Progesteron (Calbiotech R - USA). Hasil uji pararelism menunjukkan adanya penurunan OD secara paralel seiring dengan besarnya pengenceran sampel. Kadar progesteron feses didapatkan, 6.282  ± 950.96 ng/gr feses (minggu ke 4 kebuntingan); 18.391,8 ± 2584 ng/gr fese (minggu ke 8 kebuntingan); 25.958, 4 ± 3.447,1 ng/gr feses (minggu ke 12 kebuntingan); 25.233,4 ± 3.306 ng/gr feses (minggu ke 16 kebuntingan); 18.238,2 ± 3.069,5 ng/gr feses (minggu ke 20 kebuntingan). Kadar progesteron feses 2 minggu setelah melahirkan adalah 516 ± 228,16 ng/gr feses sedangkan progesteron kambing PE tidak bunting adalah 254,2 ± 214,5 ng/gr feses. Kesimpulan kadar progesteron kambing PE selama kebuntingan dapat dideteksi menggunakan sampel feses dengan kadar tertinggi pada minggu ke 12 kebuntingan. Kata Kunci: Feses; Kambing PE, Non-invasif, Progesteron
The Hematological and Biochemical Profiles of Wonosobo Sheep Blood in Various Physiological Conditions Sarmin Sarmin Sarmin; Pudji Astuti; Claude Mona Airin
Buletin Peternakan Vol 46, No 3 (2022): BULETIN PETERNAKAN VOL. 46 (3) AUGUST 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i3.73423

Abstract

The study aimed at examining the hematological and biochemical profiles of Wonosobo sheep blood in various physiological conditions. There were 32 Wonosobo sheep in the various physiological conditions, including 17 ewe lamb of 1-2 months of age, 5 pregnant female sheep of 12-48 months of age at 3 months of pregnancy, 5 ewe of 12-48 months of age in second lactation period of 1-2 months and 5 ram of 12-48 months of age. Each of the sheep was put in individual sheepfold and given Pakchong-1 grass, dried kangkong, and concentrate. Drinking water was given ad libitum. Blood sample was drawn from jugular vein and put into tubes for blood hematological and biochemical analyses. The results of the study showed that total leukocyte and lymphocyte were high in young sheep, while neutrophils was high in pregnant female sheep (P<0.05). There was not any significant physiological impact on hemoglobin, MCV, MCH, MCHC, RDW, hematocrit, platelet, monocyte, eusinophil, and basophil (P>0.05). The highest ALP activity and Pi levels were found in the ewe lamb, while low creatinine was found in the ram (P<0.05). The parameter of the activities of SGPT, SGOT, LDH, calcium, kalium, chloride, sodium, magnesium, TIBC, UIBC, TS, globulin, albumin, total protein, BUN, creatinine, glucose, cholesterol, triglyceride, HDL and LDL was not affected by physiological conditions (P>0.05). It was concluded that the impact of the physiological conditions of the Wonosobo sheep caused high total leukocyte and lymphocyte in ewe lamb and high neutrophil in pregnant female sheep, the increase in the Pi level and ALP activity of the ewe lamb, and high creatinine in the ram