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All Journal Media Veteriner Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Journal of Veterinary and Animal Sciences

I Gusti Ketut Suarjana

Laboratorium Bakteriologi Dan Mikologi Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali

Author-ID : 297707

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Uji Kepekaan Streptococcus spp. yang Diisolasi dari Penyakit Saluran Pernapasan Kompleks Babi terhadap Kanamycin, Streptomycin dan Doxycycline Dwi Arso Purba; Ketut Tono Pasek Gelgel; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Respiratory diseases in pigs often occur in the production of modern pigs around the world and known as Porcine Respiratory Disease Complex (PRDC). Prevention of PRDC can only be done if the infecting agent is bacteria. The bacteria that are most often isolated in complex pig duct disease are Streptococcus spp. This study was conducted to determine the sensitivity pattern of Streptococcus spp. isolated from animals with PRDC symptoms on kanamycin, streptomycin and doxycycline. Isolates were taken from the results of the PRDC case study of pig origin in Tabanan, Gianyar and Badung regencies. Pure isolates were then planted in blood agar and a sensitivity test was carried out using the Kirby-Bauer method for each antibiotic. Bacteria can be resistant, intermediate and sensitive to antibiotics tested. This gives rise to a pattern of sensitivity to antibiotics so that resistant germs will cause treatment failure. The results showed that hemolytic ? Streptococcus isolates were 60% sensitive to kanamycin, 100% sensitive to streptomycin, 40% resistant to kanamycin and 100% resistant to doxycycline. While hemolytic ? Streptococcus isolates were 100% sensitive to kanamycin and streptomycin but 100% were resistant to doxycycline.

Pengaruh Lama Penyimpanan Pada Suhu Kamar Telur Itik Segar Dan Telur Yang Mengalami Pengasinan Ditinjau Dari Jumlah E coli ( EFFECT OF LONG STORAGE IN THE ROOM TEMPERATURE FRESH DUCK EGGS AND DUCK EGGS IN SALTING PROCESS VIEWED FROM TOTAL OF E. coli Ratna Pandu Finata; Mas Djoko Rudyanto; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.278 KB)

This study aims to determine the number of bacteria Escherichia coli (E. coli) in fresh eggsand eggs in the salting process that is derived from UKM Mulyo, Mojokerto. A total of 24 day-oldduck eggs for the treatment of 12 fresh eggs and 12 eggs salting process. Examination of E. coli onthe first day, to eight, to 15, and day 22. Inspection is done by planting in the media EosinMethylene Blue Agar (EMBA). The results showed that the amount of E. coli on fresh eggs andegg salting increased on the first day until the eighth day, whereas on day 15 to day 22 decreased.Kesimpulanya that the amount of E. coli bacteria in fresh eggs and egg in the process pengasian increase until the eighth day and decreased on day 22.

Perbandingan Jumlah Bakteri Coliform dan Non-Coliform yang Diisolasi dari Feses Ayam Petelur pada Berbagai Kelompok Umur Made Tubagus Dharmajaya; I Gusti Ketut Suarjana; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study was conducted to compare the number of coliform and non-coliform bacteria in laying feces on various age groups. This study used a Randomized Block Design (RBD) with four age treatments, namely the starter, grower, developer, and layer phases, respectively. Each treatment used six replications, with a total of 24 samples. The samples obtained were diluted with 10-5 dilutions and planted on Eosin Methylene Blue Agar (EMBA) media. After incubating for 18-24 hours at 37 ° C, the bacteria count was then calculated. Data on the number of coliform and non-coliform bacterial colonies grown on EMBA media were analyzed for variety and continued with BNT test when it was significantly different. The results showed that the number of coliform and non-coliform bacteria in laying hens in various age groups was not significantly different.

Pengobatan Penyakit Pernapasan pada Babi dalam Upaya Meningkatkan Produktivitas Ternak di Desa Penarukan, Kerambitan, Tabanan I Gusti Ketut Suarjana; I Nengah Kerta Besung; Aida Lousie Tenden Rompis; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 12 No. 1 Pebruari 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Community service has been conducted on the treatment of respiratory diseases in pigs in an effort to increase livestock productivity in Desa Penarukan, Kerambitan, Tabanan. This dedication aims to provide knowledge to the community about how to manage pigs intensively, the prevention and eradication of diseases of pigs effectively and efficiently so that the maximum production of the livestock. Methods of service include house-to-house acts, especially to sickly breeders, initiating treatment with antibiotics, worm medicines, vitamins and minerals and providing counseling on how to prevent respiratory respiratory complex desease (PRDC) disease in pigs known as porcine respiratory complex desease (PRDC). The result obtained as follows: the community is give a positive response to activities and teams held home-to-home services to tens of breeders pigs whose reportedly suffered from PRDC spread over five banjar adat in Penarukan village. The sick pigs generally ranged from 1 to 3 months and treated as many as 20. Furthermore, based on the monitoring of the team and the report of breeders on the seventh day after being given treatment of the pigs showed no clinical symptoms and was declared cured.

Sensitivitas Isolat Escherichia coli Patogen dari Organ Ayam Pedaging Terinfeksi Koliseptikemia terhadap Oksitetrasiklin, Ampisilin dan Sulfametoksazol Yusmaniar Galuh Adi Luhung; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (687.978 KB)

This study aims to determine the sensitivity pattern pathogenic E. coli against antibiotics: oxytetracycline, ampicillin and sulfamethoxazole, in broilers in the Mangesta Village, Penebel, Tabanan Regency. The samples used in this research were 15 samples of colisepticaemia infected broiler’s organs . Sampels were isolated on media EMBA then identified with a Gram staining and biochemical tests with a TSIA, SIM, SCA and MRVP. Positive isolates of the bacterium E. coli were tested sensitivity to antibiotics oxytetracycline, ampicillin and sulfamethoxazole. The results showed that the sensitivity test of E. coli bacteria were isolated from the organs broilers suffered from colisepticaemia: 73.3% of E. coli resistant, 20% intermediate, and 6.7% was sensitive to the antibiotic oxytetracycline, 100% resistant to the antibiotic ampicillin, 53.3% and 46.7% intermediate resistance against sulphamethoxazole.

Analisis Marka Gen Patogenik hlyF pada Escherichia coli Penyebab Kolibasilosis pada Ayam Buras I Gede Eka Chandrawan; Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Colibacillosis by Avian Pathogenic Escherchia coli (APEC) is an infectious disease in poultry. The ability of APEC to cause disease depends on various virulence factors, including hlyF. The study aimed to prove the existence of the APEC hlyF gene in Bali and to find out the DNA sequences of these hlyF genes and their kinship with the hlyF gene from other countries. This study used two APEC isolates from free range chicken in Tabanan and Badung regencies. The isolates have been purified and were available at the Bacteriology Laboratory of Veterinary Medicine Faculty, Udayana University. DNA isolates were isolated using Chelex 10%. The hlyF gene is amplified using published DNA primers by polymerase chain reaction (PCR). PCR products were sequenced at First Base Laboratories in Malaysia using the Sanger’s Dideoxy Nucleotide Termination method. Both the hlyF gene sequences that can be analyzed have 100% homology with a readable length of 518 bp. Phylogenic test with 25 DNA sequences of hlyF gene on Escherichia coli and other bacteria in the world using UPGMA method with bootstrap (500 repetitions) was conducted with MEGA 5.2. All data have six polymorphic sites of nucleic acid and two polymorphic sites of amino acid . The hlyF gene from Bali was in same group with hlyF genes from various countries in the world. This gene can be used as a pathogenic marker of APEC in Indonesia.

Isolasi dan Identifikasi Bakteri Streptococcus spp. pada Babi Penderita Porcine Respiratory Disease Complex Ni Kadek Meita Swandewi; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 13 No. 2 Agustus 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Streptococcus sp. is one of the primary causes of the Porcine Respiratory Disease Complex (PRDC). This research was conducted to study the bacteria Streptococcus sp. in the pig's respiratory tract sufferers Porcine Respiratory Disease Complex (PRDC) and distribution of the bacteria Streptococcus sp. in pre-weaned and post-weaned pigs. A total of 43 samples were nasal swab taken from pigs that showed PRDC symptoms such as depression, anorexia, dyspnea, the presence of exudates from the nasal, coughing / sneezing, and swelling of the joints. Samples were received from pig farms in Tabanan regency, Badung regency, and Gianyar regency. All samples were planted on sheep media so that blood was continued with the Gram staining test. The suspected colonies were then subjected to a primary test consisting of catalase and oxidase tests as well as biochemical tests with MRPV, TSIA, SIM, coagulase test and sugar test. The results showed that 23 samples (thirteen from pre-weaned pigs and ten from post-weaning pigs) were tested positive for Streptococcus sp. hemolytic ? (20 isolates) and Streptococcus sp. hemolytic ? (3 isolates).

Deteksi Bakteri Berpotensi Patogen Pada Penyakit Saluran Pernapasan Komplek Babi I Gusti Ketut Suarjana; I Nengah Kerta Besung; Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims to detect bacterial infectious agents in pigs with complex respiratory tract disease known as porcine respiratory disease complex (PRDC) in some pig herds in Bali and look for the right medication is use to handle the incidence. The research sample comes from Tabanan, Badung and Gianyar totaling 48 Samples were isolated and identified by methods: isolation, the primary test, biochemical test. Test of sensitivity to antibiotics using disc diffusion method according to Kirby-Bauer method. The result showed that bacterial agents isolated from pigs were among others Streptococcus spp. alfa hemolytic non-coagulase (41,6%), Staphylococcus spp. beta hemolytic noncoagulase (41,6%) and Escherichia coli. Staphylococcus spp. beta hemolytic non-coagulase and E. coli were sensitive to streptomycin, kanamycin, doxicyclin, clindamycin and bacitracin. To conclude, Streptococcus spp. alfa hemolytic non-coagulase were sensitive to streptomycin, kanamycin, clindamycin and bacitracin, but resisten to doxicyclin.

Prevalensi Pasteurella multocida Pada Sapi Bali Di Bali I Nengah Kerta Besung; Ketut Tono PG; Aida Louis Tenden Rompis; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.772 KB)

This study aims to determine the prevalence of Pasteurella multocida in bali cattle in Bali. A total of 300 cows were taken from nasal swabs for isolation and identification of bacteria P.multocida. The samples came from Badung, Denpasar, Gianyar, and Bangli . Samples were grown on blood agar media. Separate colonies were identified by morphology, Gram stain, , Triple sugar Iron Agar, Methyl Red-Voger Proskauer (MRVP), Simmnons Citrate agar, Sulphid Indol Motility, sugars test and catalase test. Germs that characterizes P. multocida analyzed descriptively. The results showed that as many as 15 samples suffer from P. multocida bacteria spread in Badung 5 cattle (5%) , Denpasar 4 cattle (8%) , Gianyar 4 cattle (8%), and Bangli 2 cattle (2%).

Isolasi Klebsiella Sp. Pada Sapi Bali Berdasarkan Tingkat Kedewasaan Dan Lokasi pemeliharaan Serta Pola Kepekaan Terhadap Antibakteri Nyoman Anandiya Ramaditya; Ketut Tono PG; I Gusti Ketut Suarjana; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Research has been conducted to know the number bacterial isolates of Klebsiella sp. from bali cattle in terms of the level of maturity and the breeding location, also its sensitivity to the antibiotic there are ampicillin, sulfamethoxazole, and oxytetracycline. 120 bali cattle's nasal swabs consisting of calves, heifers, and adult cattle which are breeding on highland and lowland has been used as the isolates in this research. The obtained nasal swab was isolated and identified, then proceed with the sensitivity test. Bacterial of Klebsiella sp. has been found in calves were nine isolates (45%), heifers were six isolates (30%), and adult cattle were five isolates (25%). While based on the breeding location in the lowland found 13 isolates (65%) and highland found seven isolates (35%). In the sensitivity test results were obtained for the antibiotic ampicillin all isolates were sensitive (100%). sulfamethoxazole resistant four isolates (20%), intermediate two isolates (10%), and sensitive 14 isolates (70%). Oxytetracycline resistant seven isolates (35%), intermediate two isolates (10%), and sensitive 11 isolates (55%). The results showed that differences in the level of maturity and breeding location of bali cattle can affect the distribution of bacteria Klebsiella sp.

Co-Authors Agnes Endang Tri H Ahmad Nuzuludin Kadri, Ahmad Nuzuludin Aida Lousie Tenden Rompis Anak Agung Gede Agung Ananta Kusuma ANITA KAROLINA SIMAMORA BERATA BERATA Bianca Violanda Junus Bolla, Nelci Elizabeth chyntia nirmalasari mantrawan Daniel Opristanta Barus Danu Suprayogo Dwi Arso Purba Dwi Astalia Zuanita Eggy Hidta Lusandika Fanayoni, Aditana Genta Dhamara Adam Putranto HARDIANTO - HERTATI ANRIANI LUBIS I Gede Eka Chandrawan I Gusti Ngurah Kade Mahardika I Kadek Juli Handriana I Ketut Berata I Ketut Suada I Made Adhi Kusuma Dwipayana I Made Kardena I Made Merdana I Made Sukada I Nengah Kerta Besung I Putu Yasmanta Primarta Putra I Wayan Aris Suharsa I Wayan Suarnata I.A.P. Apsari IB. KADE SUARDANA Ida Bagus Oka Winaya Ike Siwi Widyaningtyas Indra Manik Pradipta Iwan Harjono Utama JERRY BIROWO K. Tono P.G Kadek Andre Sulaksana Kadek Satria Adi Marhendra KARTINI HUTASOIT Ketut Tono Pasek Gelgel Leny Beatry Veronica Luh Made Sudimartini M Hasan Isnan Made Deddy Dharmana Putra Made Tubagus Dharmajaya Magfirah Syahruddin Makselina Ayu Dwi Purwanti MARGARET ANASTASIA MANULLANG Marlissa, Faccettarial Cylon Marchel MAS DJOKO RUDYANTO Matilda Krisnawati MUHAMMAD RHIYAN SAKTI N.K. Suwi N.N. Suryani Nazara, Agustina Lesmauli Ni Kadek Meita Swandewi Ni Ketut Suwiti Ni Putu Nicky Mirahsanti Nyoman Anandiya Ramaditya P. Suastika Paramita, Putu Wahyuni Pratanto, Aditya Putri Udayani Putu Henrywaesa Sudipa Ratna Pandu Finata Rusmin Indra Septian Naria Rusmaniarno Sousa, Rojelio Dias Trindade Tania Ria Gunawan Trisno, Komang Voni Cornelia Br Sembiring Wati, Ni Komang Desi Yena Widya Asmara Yuniarti Sasmita Yusmaniar Galuh Adi Luhung

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