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All Journal Buletin Peternakan VALENSI Jurnal Lahan Suboptimal AL KAUNIYAH Indonesian Journal of Agricultural Science Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Livestock and Animal Research Jurnal Biodjati Jurnal Sains Teh dan Kina Jurnal Kimia Terapan Indonesia Jurnal Mikologi Indonesia Indonesian Aquaculture Journal Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Open Access DRIVERset Makara Journal of Science
Nani Radiastuti
Program Studi Biologi, FST Universitas Islam Negeri Syarif Hidayatullah, Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Veterinary Other

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ISOLATION AND IDENTIFICATION OF BACTERIA REMOVING NITRITE, NITRATE, AND AMMONIUM FROM BIOBALLS FILTER Qoriatul Ilma; Achmad Dinoto; Ninu Setianingrum; Mulyadi Mulyadi; Dwi Agustyani; Nani Radiastuti; Heddy Julistiono
Indonesian Aquaculture Journal Vol 17, No 1 (2022): (June, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.1.2022.13-22

Abstract

The presence of effective bacteria removing nitrite, nitrate, and ammonia in a recirculating aquaculture system (RAS) is necessary to attenuate their toxicity to fish. The research was conducted to find bacteria that can be cultured and reduce nitrite, nitrate, and ammonium. Sixteen bacterial colonies were isolated from bioballs of RAS biofilter and tested for their ability to reduce nitrite or nitrate concentrations. Using a simple indicator paper for nitrite and nitrate, four isolates that reduced nitrite and nitrate concentrations, namely K1NA3, K2NA3, CNA1, and PRO4NA1 were selected. The four isolates were then evaluated for the metabolism of nitrate, nitrite, and ammonium compounds using the spectrophotometry method. Results showed that the isolates K1NA3, CNA1, and PRO4NA1 reduced nitrite concentration but produced ammonium, whereas K1NA3 isolate was able to reduce nitrate concentration but produced both nitrite and ammonium. Experiments in reducing ammonium levels in the synthetic waste media showed the ability of four isolates to reduce ammonium levels after six days despite producing nitrite. Based on the 16S rRNA gene analysis, these isolates have a close relationship to Pseudomonas otitidis (KINA3 and K2NA3), Acinetobacter cumulans (CNA1), and Vogesella perlucida (PRO 4NA1).
The Potential of Nutmeg’s Microbes (Myristica fragrans Houtt.) as Antagonistic Agents against Rigidoporus microporus Dwi N Susilowati; Sri Rahayuningsih; Indah Sofiana; Nani Radiastuti
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 10 No. 1 (2021): JLSO
Publisher : Research Center for Suboptimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/JLSO.10.1.2021.529

Abstract

This study aimed to obtain yeast and bacteria from Myristica fragrans Houtt., which have the potential to produce chitinase enzymes with antagonistic ability against Rigidoporus microporous. Both microorganisms were extracted from the leaves and fruit of nutmeg. A total of 35 yeast and 29 bacterial isolates were obtained, with different morphological characters. The chitinolytic test was carried out qualitatively, and the parameters observed include the clear zones around the colony. A total of 4 bacterial isolates produced chitinase enzymes (BP 1.2.1, BP 2.1.1, EPBj II.K1, and EPBj II. K2) with a chitinolytic index of 3.92, 5.38, 2.00, and 1.66, respectively. Yeast isolates were negative for chitinase enzymes. The difference in index value indicated a variation in enzyme activity. The antagonist test was carried out using a dual culture method. A total of 1 yeast and 14 bacterial isolates inhibited the growth of R. microporous, and each has a different inhibitory zone. Based on the percentage of inhibition value, the highest percentage occurred in P.K1(41.1%), P. K2 (50%), dan EPBj II. K6 (42.2%). The antagonist test indicator includes the formation of inhibitory zones on the medium. Hemolysis test showed that yeast and bacteria are not able to break down blood cells in the medium. The molecular identification showed that P. K1 and P. K2 isolates were classified as Bacillus subtillis and EPBj II. K6 were identified as Bacillus aerius with 100% sequence homology and 99% bootstrap value respectively. These findings provided information about potential microbes that control white root fungus.       
Fungal Community of Culturable Fungal Endophytes Associated with Cinchona calisaya Collected from Gambung, West Java, Indonesia Hidayat, Iman; Radiastuti, Nani; Rahayu, Gayuh; Okane, Izumi
Makara Journal of Science
Publisher : UI Scholars Hub

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Abstract

Previous studies regarding endophytic fungi associated with Cinchona were focused on the analysis of quinine and related fungal metabolites. In the current study, the community structure of culturable endophytic fungi associated with C. calisaya (i.e., leaf, petiole, twig, root, flower, and fruit) were analyzed and elucidated. Representative isolates from various morphotypes were identified based on a sequence generated from the Internal Transcribed Spacer (ITS) rDNA region. A total of 638 fungal strains were isolated from 700 plant segments. All isolates belong to the phylum Ascomycota and are dominated by a member of Sordariomycetes. The fungal endophyte community within C. cali-saya consists of 23 known species, 10 species complexes, and 6 unidentified species. Based on the Shannon-Wiener diversity index, the leaves and fruits host the most diverse endophytic fungi, followed by twigs, petioles, and bark. Members of the genus Diaporthe are commonly found in all C. calisaya organs. Neofussicoccum sp. was only one found within twigs; the Colletotrichum gloeosporioides complex and Guignardia mangiferae were only found within leaves; the C. brasiliense complex was detected in flowers; and Fusarium oxysporum is specific to roots.
Co-Authors A Sa’duddin Achmad Dinoto Adeng Hudaya Aina Nadila Alfida Zakiyah, Alfida Amelia Rakhmaniar Anna Muawanah Dede Sukandar Dianna Rossyta Pratiwi Dwi Agustyani Dwi N Susilowati Dwi Ningsih Susilowati Elpawati, Elpawati Endang Tri Margawati Endang Tri Margawati F Wijayanti Gayuh Rahayu Gusniar Berty Hajar Indra Wardhana Heddy Julistiono Ika Roostika Ika Roostika Tambunan Iman Hidayat Indah Sofiana Indri Garnasih Ira Djajanegara Ira Djajanegara Ira Jayanegara Izumi Okane, Izumi Khoeriyah Khoeriyah La Ode Sumarlin Lisca Puji Rustanti Mulyadi Mulyadi Ninu Setianingrum Nurhasni Nurhasni Qoriatul Ilma R Fathoni Reno Fitri Rina Ningtiyas Sena Yunia Saputri Setiyani, Alfi Dwi Slamet Diah Volkandari Slamet Diah Volkandari Sofiana, Indah Sri Rahayuningsih Suminar S. Achmadi Suryadi, Yadi Susilowati, Dwi Ningsih