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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Biosaintifika: Journal of Biology & Biology Education
Bambang Tri Rahardjo
Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Environmental Science

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PENGARUH POLA PENGELOLAAN HAMA TERHADAP POPULASI SERANGGA HAMA PADA LAHAN KEDELAI VARIETAS ANJASMORO DAN WILIS Puspitasari, Mahardika; Hidayat, Purnama; ., Pudjianto; ., Marwoto; Rahardjo, Bambang Tri
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.077 KB) | DOI: 10.23960/j.hptt.11625-34

Abstract

Effect of pest management systems to the insect pests population on Anjasmoro and Wilis soybean varieties. The integrated pest management (IPM) is a pest control approach which is based on ecological and economic considerations. The objective of this research was to study the effect of different pest management systems on the insect pest population using two soybean varieties, Anjasmoro and Wilis.The experiments were done in a randomized complete block design, repeated twice. The pest management systems under the different plots were integrated pest management, non-chemical control, chemical control, and the control (which received neither chemicals nor IPM). Observations were made on the pest population and the intensity of the damage caused by primary soybean pests. The primary pests observed were Ophiomya phaseoli, Aphis gossypii, Lamprosema indicata, Spodoptera litura, Bemisia tabaci, Riptortus linearis and Etiella zinckenella. Integrated pest management significantly affect the population of whitefly on Anjasmoro variety but not on Wilis variety. The populations of bean fly, aphid, leaf roller, armyworm, stink bug and pod borer were not affected by pest management system. The lower yield of Anjasmoro variety was attributed to the attack by a viral infection whose disease incidence reached 80% on the plots without a pest management system. IPM management system was more efficient than chemical management system.
The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae) Purwaningtyas, Presti Mardiyani; Rahardjo, Bambang Tri; Tarno, Hagus
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5067

Abstract

Parasitic Root-Lession nematode of Pratylenchus coffeae can reduce the Indonesian coffee plants productivity. Several studies reported that Pseudomonas fluorescens and Bacillus subtilis endophytic bacteria were antagonistic bacteria to nematode. The objective of this research was to reveal the effectiveness of bacterial colonies density of P. fluorescens (UB_Pf1), B.subtilis (UB BS1), and a combination of both bacteria on nematode mortality using median lethal concentration (LC50) and median lethal time 50 (LT50). The densities of bacteria used in this study were 107, 109, 1011 and 1013 cfu/ml. 35 testing nematodes were used and the mortality was counted at 6, 12, 24, 36, and 48 hours after treatments. The results showed that LC50 values of P. fluorescens was (UB_Pf1) was 4,3x108 cfu/ml, LC50 B. subtilis (UB_Bs1) was 1,9x109cfu/ ml, and LC50 combination of both bacteria was, 8x107 cfu/ml. It implies that the application of the combination of both bacteria are more pathogenic than single bacterial treatment. The results also showed that the highest LT50 value was 13.21 hours combination of bacterial colonies with a density of 1013 cfu/ml and the lowest LT50 value was 52.00 hours on P. fluorescens (UB_Pf1) treatment with colonies density of 107 cfu/ml.How to CitePurwaningtyas, P. M., Rahardjo, B. T., & Tarno, H. (2016). The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae). Biosaintifika: Journal of Biology & Biology Education, 8(3), 286-293.
SAGO BARUK PALM (Arenga microcarpha Becc) AS A SUPERIOR LOCAL FOOD SOURCE AND SOIL CONSERVATION PLANT AT SANGIHE ISLAND REGENCY Marianus, Marianus; Ashari, Sumeru; Rahardjo, Bambang Tri; Polii-Mandang, Bobby
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.119

Abstract

Sagu Baruk Palm (Arenga microcarpha) is an endemic plant and producing carbohydrates as main source of local food to the people in Sangihe Island . The aim of this study is to analyze the production of  the sago and to analyze its the chemical composition and nutritional value. The research was conducted from October 2010 to June 2011 at Gunung Village Tabukan Tengah Regency Sangihe district Island. The village is spread from the coast up to the top of the hill with an altitude of ± 600 meters above sea level. The data analysis techniques in this research is descriptive analysis, F test, ANOVA.The results showed that the production of Sagu Baruk Palm at the third height position for the stem lenght 9.7-11m and stem diameter 14.3-15.7cm obtain wet sago flour 44-44.3kg and the stem rendemen from 21.3% to 22.3%, statistically only stem weight and pith weight has a significant different. The highest stem weight obtained at the bottom position, the highest pith weight obtain at the top position, and for the higest stem rendemen obtain at the top position . The chemical composition were significant differences in levels of protein, calcium, iron, magnesium, and pH at the three height positions.Key words: Superior local food sources, stem rendemen, production, sagu baruk palm.
EFFECT OF NATURAL HABITAT ON DIVERSITY OF HEMIPTERAN PREDATOR IN OIL PALM PLANTATION Rizali, Akhmad; Himawan, Toto; Fitriani, Ima; Rahardjo, Bambang Tri; Karindah, Sri; Puspitarini, Retno Dyah; Sahari, Bandung
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.923 KB) | DOI: 10.23960/j.hptt.11875-82

Abstract

Effect of Natural Habitat on Diversity of Hemipteran Predator in Oil Palm Plantation. Utillization of natural enemies in controlling oil palm pests still encounters obstacles, in particular, how natural enemies can survive and establish in oil palm plantations. The existence of natural habitats around oil palm plantations can allegedly support the occurence of natural enemies. The objective of this study was to investigate the effect of the presence of natural habitats on diversity of hemipteran predators in oil palm plantations. Field research was conducted in oil palm plantations located in Pangkalan Bun, Central Kalimantan. The plots were oil palm plantations with different distances from natural habitat i.e. near (less than 200 m), medium (about 2 km) and far (about 5 km). Each plot was selected six trees for fogging using insecticide with active ingredient lamda cyhalothrin. Based on research result, diversity of hemipteran predator in oil palm plantion was found 19 species belong to 2 families. The most dominant hemipteran predator was Sycanus sp., Eocanthecona sp. and Reduviidae sp5. The distance of oil palm plantation from natural habitat did not affect species richness, abundance and composition as well as recolonization of hemipteran predator in oil palm plantation. Abundance of hemipteran predator tend to decrease in different observation time, except Sycanus sp. Sycanus sp was likely has ability to rapidly recolonized and their occurrence were influenced by flowering vegetation in oil palm plantation.
Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Tanor, Meity N; Abadi, Abdul Latief; Rahardjo, Bambang Tri; Pelealu, Jantje
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

Abstract

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).
Silica Fertilizer (Si) Enhances Sugarcane Resistance to The Sugarcane Top Borer Scirpophaga excerptalis Walker Rahardjo, Bambang Tri; Achadian, Etik Mar`ati; Taufiqurrahman, Achmad Fitriadi; Hidayat, Mohamad Rizkiy
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v1i1.2654

Abstract

Sugarcane is an essential industrial that produce sugar in the world. Scirpophaga excerptalis attacks can reduce the productivity of sugarcane up to 34%. To enhance the resistance to multiple stresses, Silicon is one of the elements for its role. This research aimed to determine the effect of the provision of silica fertilizer as part of the Integrated Pest Management strategy. This research was conducted in the Plant Protection Department, Indonesian Sugar Research Institute. The compost given as treatment in this research was straw, cane, and corn composts. Organic silica fertilizer (compost) provides the same effect as inorganic silica fertilizer in increasing the induction of sugarcane resistance to sugarcane top borer (S. excerptalis). Rice straw compost was a compost fertilizer that provides the best sugarcane resistance to sugarcane top borer compared to sugarcane plants that treated with sugarcane and corn leave to compost
Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Pramudi, Muhammad Indar; Puspitarini, Retno Dyah; Rahardjo, Bambang Tri
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
Co-Authors A. A. Ketut Aryawan Abdul Latief Abadi Achadian, Etik Mar`ati Ahmad Muhlisin Akhmad Rizali Ayu Rosmayuningsih BANDUNG SAHARI Bobby Polii Dede Durahman Fitriani, Ima Guntur Respyan Hagus Tarno Hagus Terno Hidayat, Mohamad Rizkiy Husni dan Zulfanazli Pudjianto Alfian Rusdy Isnainy Dinul Mursyalati Yus Jantje Pelealu Liza Afifah Ludji Pantja Astuti Marianus Marianus Marwoto . Maspupah Huzni Meity Nelltje Tanor Muhammad Indar Pramudi Presti Mardiyani Purwaningtyas, Presti Mardiyani PURNAMA HIDAYAT Retno Dyah Puspitarini Rina Rachmawati Rizki Puji Widiastuti Ronny Pamuji Sri Karindah Sumeru Ashari Taufiqurrahman, Achmad Fitriadi Toto Himawan Zeni Ningrum