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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Buletin Tanaman Tembakau, Serat & Minyak Industri Jurnal Pembangunan dan Alam Lestari Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Buletin Tanaman Tembakau, Serat & Minyak Industri Jurnal HPT (Hama Penyakit Tumbuhan)

Bambang Tri Rahardjo

Jurusan Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya

Author-ID : 354501

Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Decision Sciences, Operations Research & Management Environmental Science

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PENGARUH POLA PENGELOLAAN HAMA TERHADAP POPULASI SERANGGA HAMA PADA LAHAN KEDELAI VARIETAS ANJASMORO DAN WILIS Puspitasari, Mahardika; Hidayat, Purnama; ., Pudjianto; ., Marwoto; Rahardjo, Bambang Tri
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Effect of pest management systems to the insect pests population on Anjasmoro and Wilis soybean varieties. The integrated pest management (IPM) is a pest control approach which is based on ecological and economic considerations. The objective of this research was to study the effect of different pest management systems on the insect pest population using two soybean varieties, Anjasmoro and Wilis.The experiments were done in a randomized complete block design, repeated twice. The pest management systems under the different plots were integrated pest management, non-chemical control, chemical control, and the control (which received neither chemicals nor IPM). Observations were made on the pest population and the intensity of the damage caused by primary soybean pests. The primary pests observed were Ophiomya phaseoli, Aphis gossypii, Lamprosema indicata, Spodoptera litura, Bemisia tabaci, Riptortus linearis and Etiella zinckenella. Integrated pest management significantly affect the population of whitefly on Anjasmoro variety but not on Wilis variety. The populations of bean fly, aphid, leaf roller, armyworm, stink bug and pod borer were not affected by pest management system. The lower yield of Anjasmoro variety was attributed to the attack by a viral infection whose disease incidence reached 80% on the plots without a pest management system. IPM management system was more efficient than chemical management system.

The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae) Purwaningtyas, Presti Mardiyani; Rahardjo, Bambang Tri; Tarno, Hagus
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5067

Parasitic Root-Lession nematode of Pratylenchus coffeae can reduce the Indonesian coffee plants productivity. Several studies reported that Pseudomonas fluorescens and Bacillus subtilis endophytic bacteria were antagonistic bacteria to nematode. The objective of this research was to reveal the effectiveness of bacterial colonies density of P. fluorescens (UB_Pf1), B.subtilis (UB BS1), and a combination of both bacteria on nematode mortality using median lethal concentration (LC50) and median lethal time 50 (LT50). The densities of bacteria used in this study were 107, 109, 1011 and 1013 cfu/ml. 35 testing nematodes were used and the mortality was counted at 6, 12, 24, 36, and 48 hours after treatments. The results showed that LC50 values of P. fluorescens was (UB_Pf1) was 4,3x108 cfu/ml, LC50 B. subtilis (UB_Bs1) was 1,9x109cfu/ ml, and LC50 combination of both bacteria was, 8x107 cfu/ml. It implies that the application of the combination of both bacteria are more pathogenic than single bacterial treatment. The results also showed that the highest LT50 value was 13.21 hours combination of bacterial colonies with a density of 1013 cfu/ml and the lowest LT50 value was 52.00 hours on P. fluorescens (UB_Pf1) treatment with colonies density of 107 cfu/ml.How to CitePurwaningtyas, P. M., Rahardjo, B. T., & Tarno, H. (2016). The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae). Biosaintifika: Journal of Biology & Biology Education, 8(3), 286-293.

SAGO BARUK PALM (Arenga microcarpha Becc) AS A SUPERIOR LOCAL FOOD SOURCE AND SOIL CONSERVATION PLANT AT SANGIHE ISLAND REGENCY Marianus, Marianus; Ashari, Sumeru; Rahardjo, Bambang Tri; Polii-Mandang, Bobby
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.119

Sagu Baruk Palm (Arenga microcarpha) is an endemic plant and producing carbohydrates as main source of local food to the people in Sangihe Island . The aim of this study is to analyze the production of the sago and to analyze its the chemical composition and nutritional value. The research was conducted from October 2010 to June 2011 at Gunung Village Tabukan Tengah Regency Sangihe district Island. The village is spread from the coast up to the top of the hill with an altitude of ± 600 meters above sea level. The data analysis techniques in this research is descriptive analysis, F test, ANOVA.The results showed that the production of Sagu Baruk Palm at the third height position for the stem lenght 9.7-11m and stem diameter 14.3-15.7cm obtain wet sago flour 44-44.3kg and the stem rendemen from 21.3% to 22.3%, statistically only stem weight and pith weight has a significant different. The highest stem weight obtained at the bottom position, the highest pith weight obtain at the top position, and for the higest stem rendemen obtain at the top position . The chemical composition were significant differences in levels of protein, calcium, iron, magnesium, and pH at the three height positions.Key words: Superior local food sources, stem rendemen, production, sagu baruk palm.

EFFECT OF NATURAL HABITAT ON DIVERSITY OF HEMIPTERAN PREDATOR IN OIL PALM PLANTATION Rizali, Akhmad; Himawan, Toto; Fitriani, Ima; Rahardjo, Bambang Tri; Karindah, Sri; Puspitarini, Retno Dyah; Sahari, Bandung
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Effect of Natural Habitat on Diversity of Hemipteran Predator in Oil Palm Plantation. Utillization of natural enemies in controlling oil palm pests still encounters obstacles, in particular, how natural enemies can survive and establish in oil palm plantations. The existence of natural habitats around oil palm plantations can allegedly support the occurence of natural enemies. The objective of this study was to investigate the effect of the presence of natural habitats on diversity of hemipteran predators in oil palm plantations. Field research was conducted in oil palm plantations located in Pangkalan Bun, Central Kalimantan. The plots were oil palm plantations with different distances from natural habitat i.e. near (less than 200 m), medium (about 2 km) and far (about 5 km). Each plot was selected six trees for fogging using insecticide with active ingredient lamda cyhalothrin. Based on research result, diversity of hemipteran predator in oil palm plantion was found 19 species belong to 2 families. The most dominant hemipteran predator was Sycanus sp., Eocanthecona sp. and Reduviidae sp5. The distance of oil palm plantation from natural habitat did not affect species richness, abundance and composition as well as recolonization of hemipteran predator in oil palm plantation. Abundance of hemipteran predator tend to decrease in different observation time, except Sycanus sp. Sycanus sp was likely has ability to rapidly recolonized and their occurrence were influenced by flowering vegetation in oil palm plantation.

Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Tanor, Meity N; Abadi, Abdul Latief; Rahardjo, Bambang Tri; Pelealu, Jantje
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).

Silica Fertilizer (Si) Enhances Sugarcane Resistance to The Sugarcane Top Borer Scirpophaga excerptalis Walker Rahardjo, Bambang Tri; Achadian, Etik Mar`ati; Taufiqurrahman, Achmad Fitriadi; Hidayat, Mohamad Rizkiy
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v1i1.2654

Sugarcane is an essential industrial that produce sugar in the world. Scirpophaga excerptalis attacks can reduce the productivity of sugarcane up to 34%. To enhance the resistance to multiple stresses, Silicon is one of the elements for its role. This research aimed to determine the effect of the provision of silica fertilizer as part of the Integrated Pest Management strategy. This research was conducted in the Plant Protection Department, Indonesian Sugar Research Institute. The compost given as treatment in this research was straw, cane, and corn composts. Organic silica fertilizer (compost) provides the same effect as inorganic silica fertilizer in increasing the induction of sugarcane resistance to sugarcane top borer (S. excerptalis). Rice straw compost was a compost fertilizer that provides the best sugarcane resistance to sugarcane top borer compared to sugarcane plants that treated with sugarcane and corn leave to compost

Hubungan Antara Profil Protein Populasi Vektor Wereng Hijau Nephotettix virescens dan Gejala Penularan Tungro pada Tanaman Padi Senoaji, Wasis; Rahardjo, Bambang Tri; Tarno, Hagus
Jurnal Penelitian Pertanian Tanaman Pangan Vol 5, No 1 (2021): April 2021
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpptp.v5n1.2021.p25-36

Nephotettix virescens is the most effective vector for transmitting tungro disease to rice plants. Two different viral particles cause Tungro transmission. Disease control was often not anticipated in the field, especially when planting is asynchronous, that cause been detected lately. At the cellular level, vector interactions with viruses indicate vector proteins response to viral in the body of vector insects which involved in virus transmission in plants. This study aims to describe the relationship between the differentiation of N. virescens vector protein profiles on the types of tungro symptoms resulting from the transmission to develop techniques for early detection and control of the transmission process. The workflow of this study is screening on vector insects to obtain protein candidates thought to have a role in tungro transmission that had never been previously reported. The results of this study suggested that proteins with estimated molecular weights of 132, 73, and 49 kDa are candidates for proteins that can be used for screening purposes or virulent vector tracing as an early warning alternative to control tungro disease in endemic areas.

Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Pramudi, Muhammad Indar; Puspitarini, Retno Dyah; Rahardjo, Bambang Tri
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA

Pengaruh PGPR terhadap Penekanan Populasi Nematoda Puru Akar (Meloidogyne incognita (Kofoid and White) Chitwood) pada Tanaman Kenaf (Hibiscus cannabinus L.) Kristiana Sri Wijayanti; Bambang Tri Rahardjo; Toto Himawan
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 8, No 1 (2016): April 2016
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bultas.v8n1.2016.30-39

Tanaman kenaf yang terinfeksi nematoda Meloidogyne incognita dapat mempengaruhi pertumbuhan tanaman dan produksi serat. Penelitian ini bertujuan untuk mengevaluasi pengaruh Plant Growth Promoting Rhizobacteria (PGPR) dalam menekan populasi nematoda M. incognita pada tanaman kenaf di rumah kaca. Penelitian dirancang dengan menggunakan rancangan acak kelompok (RAK) faktorial dengan dua faktor, faktor pertama adalah cara aplikasi PGPR yang terdiri atas 2 cara yaitu suspensi PGPR diberikan sebelum tanam dengan merendam benih selama 5 jam (C1), benih ditanam langsung dalam pot tanpa direndam dalam PGPR (C2), dan suspensi PGPR diberikan pada 15 hari setelah tanam (HST) dan 25 HST. Faktor kedua adalah jenis PGPR yang digunakan yaitu Pseudomonas fluorescens, Bacillus subtilis, Azotobacter sp., P. fluorescens + B. subtilis, P. fluorescens + Azotobacter sp., B. subtilis + Azotobacter sp., dan P. fluorescens + B. subtilis + Azotobacter sp., serta kontrol (tanpa PGPR). Hasil penelitian menunjukkan bahwa perendaman benih dengan kombinasi tiga bakteri memberikan pengaruh yang nyata terhadap populasi juvenil nematoda dalam tanah, sedangkan perlakuan tanpa perendaman tidak memberikan pengaruh. Populasi juvenil nematoda di dalam akar yang diberi PGPR baik tunggal maupun kombinasi melalui perendaman benih atau tanpa perendaman benih tidak berpengaruh, kecuali pada kombinasi P. fluorescens dan B. subtilis yang diberikan melalui perendaman benih mampu menekan populasi juvenil nematoda di akar 43,28% bila dibandingkan tanpa perendaman benih. Pemberian rizobakteri P. fluorescens menurunkan jumlah telur nematoda terbanyak (86,39%) dan menekan intensitas penyakit sebesar 71,95% bila dibandingkan kontrol.Infection of Meloidogyne incognita on kenaf could affect its growth and the production of fiber. This study aimed to evaluate the effect of PGPR on the reduction of nematode M. incognita population on kenaf in the greenhouse. The factorial experiment was laid on randomized block design. The study consisted of two factors with three replicates . The first factor was method of PGPR application, ie: PGPR suspension was given before planting (kenaf seeds was soaked for 5 hours) (C1) and the seeds directly planted without submerged (C2), PGPR suspension was given at 15 days after planting (DAP) and 25 dap. The second factor was type of bacteria (Pseudomonas fluorescens, Bacillus subtilis, Azotobacter sp., P. fluorescens + B. subtilis, P. fluorescens + Azotobacter sp., B. subtilis + Azotobacter sp., and P. fluorescens + B. subtilis + Azotobacter sp.) and control. The results showed that submerged seed with the three bacterial rhizobacteria significant compared to the control treatment and single treatment and two combination rhizobacteria, while without submerged seed with single or combination rhizobacteria not significant on the population of juvenile nematodes in the soil. Combination of P. fluorescens and B. subtiliswith submerged seed capable of suppressing the population of juvenile nematodes in the roots of 43.28% when compared with or without submerged seed. Population of juvenile nematodes in the roots by submerged seed and without submerged seed either single or combination rhizobacteria do not affect each other. P. fluorescens suppress nematode eggs are highest 86.39% and disease intensity by 71,95% where compared to control.

Endophyte Initiation Beauveria bassiana (Balsamo) Vullemin (Hypocreales: Cordycipitaceae) in Reduction of Aphis glycine Matsumura (Hemiptera: Aphididae) Investation in Soybean (Glycine max (L.) Merrill) Plant Mufidah Afiyanti; Rina Rachmawati; Achmad Faisal Akbar; Rose Novita Sari Handoko; Bambang Tri Rahardjo
Indonesian Journal of Environment and Sustainable Development Vol 10, No 1 (2019)
Publisher : Graduate Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2019.010.01.04

Soybean (Glycine max (L.) Merrill) is a universal important plant. Its production has decreased each year. A major problems is a pest known as Aphis glycine Matsumura (Hemiptera: Aphididae) which caused the decline of yield up to 40%. Thus, there is a need to control A. glycine. Recent studies showed B. bassiana has a potency as endophyte in Vicia faba (Fabaceae) plant tissue. It could harm A. gossypii and causes death up to 57,7%. Therefore, the aim of this research is to control infestation of A. glycine by using B. bassiana. This research used Randomized Block Design with soybean plant Anjasmoro variety as samples. The initiation of B. bassiana was conducted by using seed-soaking and soil-wetting methods. The results showed that fungus B. bassiana was able to colonize soybean plants with a percentage of colonization reaching 6.67%. Colonization was only found in the stems of soybean plants in the seed immersion treatment with the susceptibility of B. bassiana fungus in density of 108 conidia / mL for 2 hours, colonization on the stem itself by 20%. Fungus density results from evaluation of 1.6 x 105 conidia / mL and conidial viability reached 87.4%. There were no deaths and changes in the population of A. glycine aphids. Direct pathogenicity testing has a significant effect on the treatment between observations of the second day. Initiation of B. bassiana fungi did not significantly affect plant height and number of leaf branches. This results conclude that B. bassiana may function as endophyte in soybean plants. However, its function in reduction of greenfly still need further researchKeywords: Biological control, Environmental pest management, Greenfly

Co-Authors A. A. Ketut Aryawan Abdul Latief Abadi Achadian, Etik Mar`ati Achmad Faisal Akbar Ahmad Muhlisin Ahmad Muhlisin Akhmad Rizali Antika Anggraini Ayu Rosmayuningsih Ayu Rosmayuningsih BANDUNG SAHARI Bobby Polii David Soetjipto Dede Durahman Dede Durahman Fitriani, Ima Guntur Respyan Guntur Respyan Hagus Tarno Hagus Terno Hidayat, Mohamad Rizkiy Husni dan Zulfanazli Pudjianto Alfian Rusdy Isnainy Dinul Mursyalati Yus Isnainy Dinul Mursyalati Yus Jami'at Dwi Elriyono Jantje Pelealu Karisma Aditya Wardani Kristiana Sri Wijayanti Kurnia Paramita Sari Liza Afifah Liza Afifah Ludji Pantja Astuti Ludji Pantja Astuti Marianus Marianus Marwoto . Maspupah Huzni Maspupah Huzni Meity Nelltje Tanor Mufidah Afiyanti Muhammad Indar Pramudi Nurul Aini Presti Mardiyani Purwaningtyas, Presti Mardiyani PURNAMA HIDAYAT Putri Setya Rahmita Retno Dyah Puspitarini Rina Rachmawati Rina Rachmawati Rina Rachmawati Rizki Puji Widiastuti Rizki Puji Widiastuti Ronny Pamuji Ronny Pamuji Rose Novita Sari Handoko Senoaji, Wasis Sri Karindah Sri Karindah Sumeru Ashari Syamsuddin Djauhari Syamsuddin Djauhari Taufiqurrahman, Achmad Fitriadi Tita Widjayanti Toto Himawan Zeni Ningrum Zeni Ningrum

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