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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Peternakan Sriwijaya Jurnal Veteriner Jurnal Ilmiah Universitas Batanghari Jambi Animal Production : Indonesian Journal of Animal Production JAS (Jurnal Agri Sains) Jurnal Nukleus Peternakan Semah : Jurnal Pengelolaan Sumberdaya Peraira Jurnal Produksi Ternak Terapan (JPTT) Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Jurnal Nusantara Mengabdi Journal of Livestock and Animal Health (JLAH)
Bayu Rosadi
Fakultas Peternakan Universitas Jambi
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The Quantity and Quality of Brahman Cross Cattle Embryo After Injected FSH and PMSG Adriani, Adriani; Rosadi, B; Depison, Depison
ANIMAL PRODUCTION Vol 11, No 2 (2009): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Twenty cattles were used in this experiment to determine the  quantity and  quality of embryo after injected FSH (follicle stimulating hormone ) and  PMSG (pregnant mare serum gonadotrophin) in Brahman Cross Cattle. The experiment was assigned into Completely Randomized Design with 5 treatments and 4 replications. The treatments were T1 = 4 mg of FSH twice a day intra-ovary decreased doses, T2 = 8 mg of FSH twice a day intra-ovary decreased doses, T3 = 300 IU of PMSG single dose intra-ovary, T4 = 600 IU of PMSG single dose intra-ovary, T5 = 40 mg of FSH twice a day intramuscular decreased doses. Trial cattle were oestrus synchronized using 15 mg of PGF2α that gave twice at 11-daily intervals. One day after giving FSH and PMSG was detected the cattle’s oestrus. Washing uterus  was done at day 7 after AI using mixture of PBS, FCS and streptomicyn. Data observed were cow performances, embryo quantity and embryo quality. Results of experiment showed that 19 cattle (95%) responded oestrus synchronized treatment and super ovulation, whereas 1 cattle (5%) did not respond oestrus synchronized treatment and super ovulation. Generally, cattle showed oestrus at 2 – 3 days after giving PGF2α. Eleven cattle (57,90%)  showed oestrus at 2 days after giving PGF2α whereas the others (8 cattle, 42,10%) showed oestrus 3 days giving PGF2α. The treatment of giving FSH and PMSG could increase (P<0,05) embryo. T5 was highest  compared the others ( T1, T2, T3 and T4), while T2 and T4 were higher than T1 and T3. Produced total embryo was 82 with average 4,3 ± 5,67 using FSH and PMSG. 8 embryo (9,76%), 9 embryos (10,90), 20  embryo (24,40%), 16 embryo (19,50%) and  29 embryos (35,40%) were grade A, B, C, D and E respectively. It is concluded that giving of 40 mg  FSH  intramusculer produce the best embryo donor whereas  and giving  of FSH 8 mg intraovari was the best effeciency. (Animal Production 11(2): 96-102 (2009) Key Words : Brahman Cross Cattle, embryo, PGF2α PMSG, FSH
The Effects of Super Ovulation on the Number of Corpus Luteum of Coastal Sumatera Cows (Bos sumatranensis) Depison, Depison; Adriani, Adriani; Rosadi, B
ANIMAL PRODUCTION Vol 13, No 1 (2011): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective of this study was to observe the effects of super ovulation treatments on the number of ovulated corpus luteum. Metode   yang digunakan dalam penelitian adalah metode eksperimen. The method used in this study was experimental. Sapi-sapi diprogram untuk sinkronisasi estrus menggunakan PGF2ά. The cows were programmed to experience estrous at relatively the same time (estrous synchronization) using PGF2ά. Sapi-sapi tersebut kemudian dibagi secara acak menjadi empat perlakuan, masing-masing terdiri dari empat ekor sebagai ulangan. Data yang dihimpun dalam penelitian ini adalah jumlah corpus luteum, dan jumlah folikel tetap. Cows were divided randomly into four treatments, each of which consisting of four heads. The number of corpus luteum and the settled follicles was collected during the study. Data dianalisis menggunakan Rancangan Bujur Sangkar Latin, jika perlakuan berpengaruh nyata maka dilajutkan dengan uji lanjut Duncan. Data were analyzed using a Latin Square design. In case there was a significant difference, Duncan test was used thereafter. Result showed that Hasil penelitian menunjukkan bahwa penggunaan PGF2ά dalam pelaksanaan sinkronisasi estrus berjalan cukup baik.Hasil penelitian ini menunjukkan bahwa perlakuan superovulasi berpangaruh nyata terhadap Corpus Luteum dan folikel menetap.super ovulation had a significant effect on corpus luteum and settled follicles. Kesimpulan penelitian ini  protokol terbaik superovulasi tahun pertama adalah T-3 karena menghasilkan Corpus luteum dan Embrio yang lebih banyak.In conclusion, the best super ovulation protocol in the first year was the combination of 4 day daily injection of 40 mg of FSH in decreasing dose plus 250 µg of GnRH given at the time of estrous, because it was able to yield greater number of corpus luteum and embryos. (Animal Production 13 (1):52-56 (2011)Key Words: corpus luteum, follicle, super ovulation, Coastal Sumatera Cow
Penggunaan Follicle Stimulating Hormone dan Pregnant Mare Serum Gonadotrophin untuk Superovulasi pada Sapi Persilangan Brahman Adriani Adriani; B Rosadi; Depison Depison
Media Peternakan Vol. 32 No. 3 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Twenty cattle were used in this experiment to determine the effect of administration follicle stimulating hormone (FSH) and pregnant mare serum gonadotrophin (PMSG) hormones on superovulation of Brahman cross cattle. The experiment was designed into completely randomized design with 5 treatments as follows. Treatments 1 (T1): 4 mg of FSH was injected twice a day intra-ovary, T2: 8 mg of FSH was injected twice a day intra-ovary, T3: 300 IU of PMSG was injected single dose intra-ovary, T4: 600 IU of PMSG was injected single dose intra-ovary, T5: 40 mg of FSH was injected intramuscular. All experimental cattle were oestrus synchronized using 15 mg of PGF2α  twice at 11-days intervals. Number of corpus luteum (CL) was detected by rectal palpation at day-7 after artificial insemination. Results showed that 19 cattle (95%) indicated oestrus sign. Eleven cattle (57.9%) showed oestrus sign 2 days after PGF2α  injection and the rest 8 cattle (42.1%) oestrus sign was detected at 3 days after PGF2α  injection. FSH and PMSG treatments increased significantly (P < 0.05) number of CL. The highest CL number was found in T5, meanwhile number of CL in T2 and T4 were higher compared to T1 and T3. The average treatment effect could produce 6.8±5.42 CL with range 2–26 CL. On the other hand single dose treatment of 600 IU PMSG (T4) showed high significant number of non ovulatory (persistent) follicle compared to other treatments (T1, T2, T3 and T5) on average number of persistent follicle 2.0±1.97 from 19 cattles. It is concluded that the best superovulation treatment was produced by injection 40 mg of FSH intra-musculary. Key words: Brahman cross, corpus luteum, PMSG, FSH
Analisis Keberhasilan Inseminasi Buatan (IB) Ternak Sapi Berdasarkan Karakteristik Inseminator di Kabupaten Kerinci Lusi Amidia; Fachroerrozi Hoesni; Bayu Rosadi
Jurnal Ilmiah Universitas Batanghari Jambi Vol 21, No 2 (2021): Juli
Publisher : Universitas Batanghari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/jiubj.v21i2.1481

Abstract

This study aims to determine the effect of internal and external inseminator characteristics on the success of IB in Kerinci Regency. This research was conducted on December 12, 2020 to January 6, 2021. The objects observed in this study were all inseminators at each IB post in Kerinci Regency.The data obtained from this study are primary data and secondary data. The data were analyzed using Stepwise Multiple Regression analysis. The results of the analysis show that the success of artificial insemination in Kerinci Regency is good, this can be seen from the S/C value per inseminator of 1.69.The influence of internal characteristics (length of work, training intensity, accuracy, technical ability of straw management and estrus detection) and external (distance from house to work area, supporting facilities, condition of post IB, sanitation of tools and fittings) has an effect (P>0.05). Meanwhile, internal characteristics (responsibility) and external (voluntary rewards) have no effect (P>0.05) on the success of IB in Kerinci Regency. Based on this research, it can be concluded that not all internal and external characteristics affect the success of IB in Kerinci Regency.
Efek Penyimpanan Epididimis Sapi Bali Pada Suhu 5? terhadap Kualitas Spermatozoa Bayu Rosadi; Liber Hasudungan Sinurat; Silvia Erina
Jurnal Peternakan Sriwijaya Vol 9, No 2 (2020): JURNAL PETERNAKAN SRIWIJAYA
Publisher : Department of Animal Sciences, Faculty of Agriculture, Sriwijaya University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/JPS.9.2.2020.10988

Abstract

Spermatozoa dari epididimis hewan yang sudah mati merupakan pilihan menarik untuk preservasi gamet jantan. Penelitian ini bertujuan mengetahui pengaruh penyimpanan epididimis pada suhu 5 0C terhadap kualitas spermatozoa sapi Bali. Sebanyak 20 buah epididimis sapi Bali disimpan dalam refrigerator dengan suhu 5 oC, dialokasikan pada 4 lama penyimpanan yang berbeda yaitu kontrol, 24 jam, 48 jam, dan 72 jam. Peubah yang diamati meliputi motilitas, persentase hidup dan abnormalitas. Dari hasil penelitian ini didapatkan bahwa lama penyimpanan pada suhu 5 oC berpengaruh nyata (P<0,05) terhadap motilitas namun tidak mempengaruhi (P>0,05) persentase hidup dan abnormalitas. Motilitas turun pada 48 jam (62,23%) dan 72 jam (59,46%) dibandingkan kontrol (70,36%), sedangkan 24 jam (67,74%) tidak berbeda dengan kontrol. Dapat disimpulkan bahwa penyimpanan epididimis pada suhu 5 oC selama 72 jam mampu mempertahankan kualitas spermatozoa sapi Bali yang layak untuk inseminasi.
DEVELOPMENT OF MICE AND HAMSTER EMBRYOS IN KSOMAA AND HECM-6 MEDIUM Bayu Rosadi; M Agus Setiadi; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of the present study was to investigate the viability of mice and hamster embryos developed in Kalium Simplex Optimized Medium amino acid (KSOMaa) and Hamster Embryo Culture Medium-6 (HECM-6) medium. Female DDY mice were superovulated by injection i.p. of 5 IU Pregnant Mare Serum Gonadotropine (PMSG) and 5 IU Human Chorionic Gonadotropine (hCG) in 48 h interval, hamster (Phodopus campbelli) injected by 2.5 IU PMSG and 2.5 IU hCG 48 h later. Then females were mated with fertile males. Eight-cell embryos were recovered at day 3 after natural mating. The mice embryos were cultured in KSOMaa+5% NBCS (New Born Calf Serum) (T1) and HECM-6+5% NBCS (T2), the hamster embryos were cultured in KSOMaa+5% NBCS (T3) and HECM-6 + 5% NBCS (T4) for further development at 37oC in a humidified atmosphere of 5% CO2 in air for 48 h. The examinations were replicated five times. The T1 embryos developed to compact morulla and early blastocyst 100% (140/140), 92.1% (129/140) to blastocyst and expanded blastocyst, and 22.9% (32/140) became hatching/hatched. The T3 reached 100% (60/60) to compact morulla and early blastocyst, 85.0% (51/60) blastocyst, and 48.3% (29/60) expanded blastocyst, no embryo observed hatching/hatced. The T2 embryos had more expanded blastocyst than T3 (P<0.05), hatching/hatched rate higher than T1 and T3 but lower than T4 (P<0.05). Shortly, KSOMaa enable to support 8-cell stage mice and hamster embryo, but the hamster embryo developed lower at expanded blastocyst stage. HECM-6 is more appropriate than KSOMaa to support 8-cell mice embryos development and suitable to develop 8-cell stage hamster embryos.
Preservasi Ovarium dan Pengaruhnya Terhadap Morfologi Folikel Domba Bayu Rosadi; Mohamad Agus Setiadi; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study was to evaluate the effect of cooling and freezing of ewes ovarian tissue ontheir follicles morphology. The study was carried out in two experiments. Experiment I, ovaries weremaintained in Phosphate Buffered Saline (PBS) at -20oC and room temperature (RT) for 24 h, and at 5oCfor 24 h and 72 h, respectively. After storage, follicles were histologically evaluated. Experiment II, theovarian cortex was isolated and tissue slices (±1 mm3) were prepared. Following this tissues were loadedinto hemistraw then transferred to equilibration solution (PBS+20% FBS+7,5% EG+7,5 % DMSO) atroom temperature and held for 10, 20, 30 minutes, respectively. Afterward tissues were tranferred tovitrification solution (PBS+20%FBS +15%EG+15%DMSO ) for 3 minutes, then the hemistraw was placeddirectly into liquid nitrogen. After thawing, the tissues were prepared for histological examination. All ofthe follicles were deteriorated after 24 h storage at RT. The percentage of morphologically normal follicleswas significantly reduced when ovarian tissues were stored at -20oC for 24 h and at 5oC for 24 h and 72 h.However, it seemed to have a minor deterioration effect when the tissues were kept at 5oC for 24 h(P<0.05). Antral follicles were damaged in all of the treatments. Primordial follicles preserved theirmorphology intactness better than growing follicles. Exposing tissues to equilibration medium for 10minutes seemed to produce higher numbers of morphologically normal follicles (P<0.05), compared towhen tissues were exposed for 20 minutes and 30 minutes (P>0.05). It can be concluded that exposingtissues to equilibration solution for 10 minutes prior to freezing would kept the ovarian follicles morphologyin good condition.
Identifikasi Gangguan Reproduksi pada Ovarium Sapi Potong yang Mengalami Anestrus Postpartum Panjang (IDENTIFICATION OF REPRODUCTIVE DISRUPTION ON PROLONGED POSTPARTUM ANESTRUS BEEF COW OVARIES) Bayu Rosadi; Teguh Sumarsono; Fachroerrozi Hoesni
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Postpartum anestrus is the major factor causing elongation of the calving interval and in consequense, lowering beef cow productivity. Anestrus condition is closey related to inactive ovary condition. The research was conducted to find out reproductive disorders in ovarian of beef cattle cow that underwent prolonged postpartum anestrus (more than 3 months). One hundred and fifty heads post partum anestrus cow were selected. The general body condition were evaluated based on Body Condition Score (BCS). The types of reproductive disruption in ovary were determined by rectal palpation method. The cows observed had 2.0 to 3.2 (2.6 ± 0.7) in BCS. The result showed that 37.68% of prolonged postparum anestrus cow had ovarian disorders. The types of ovarian disoreders detected were hypofunction (19.32%), cystic follicle (8.21%), persistent corpus luteum (4.5%), atrophy (1.93%) and partial agenesis (0.97%).
PERBANDINGAN HASIL TANGKAPAN UDANG MENGGUNAKAN ALAT TANGKAP SONDONG PADA UKURAN MATA JARING YANG BERBEDA DI KELURAHAN TANJUNG SOLOK KECAMATAN KUALA JAMBI Suhardi Pratama; Bayu Rosadi; Afriani H
SEMAH Jurnal Pengelolaan Sumberdaya Perairan Vol 5, No 2 (2021): Desember 2021
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/semahjpsp.v5i1.728

Abstract

Tanjung Solok memiliki perairan estuaria dengan dasar pantai yang landai dan memiliki substrat berlumpur yang menjadikan daerah Tanjung Solok sebagai salah satu sentra dalam penangkapan udang. Salah satu alat tangkap yang digunakan untuk penangkapan udang di Kelurahan Tanjung Solok adalah alat tangkap sondong. Penelitian ini bertujuan untuk mengetahui perbandingan hasil tangkapan dan komposisi udang menggunakan alat tangkap sondong dengan ukuran mata jaring yang berbeda di Kelurahan Tanjung Solok. Penelitian ini dilaksanakan pada tanggal 24 November sampai 18 Desember 2020 bertempat di Kelurahan Tanjung Solok Kecamatan Kuala Jambi. Metode penelitian yang digunakan adalah metode survei. Teknik penentuan lokasi menggunakan metode purposive sampling sedangkan penentuan responden diambil masing-masing satu nelayan (ukuran mata jaring 1 inchi diambil satu nelayan dan ukuran mata jaring 1 ¼ inchi satu nelayan) dengan frekuensi pengambilan data sebanyak 16 kali penangkapan pada setiap ukuran mata jaring yang berbeda. Hasil penelitian menunjukkan bahwa tangkapan sondong selama 16 kali penangkapan ukuran mata jaring 1 inchi memperoleh jumlah tangkapan sebanyak 36.166 ekor dengan bobot sebesar 340,80 Kg sedangkan ukuran mata jaring 1 ¼ inchi memperoleh hasil tangkapan sebanyak 28.795 ekor dengan bobot sebesar 271,10 Kg. Berdasarkan penelitian ini dapat disimpulkan bahwa hasil tangkapan udang menggunakan alat tangkap sondong ukuran mata jaring 1 inchi lebih banyak dibandingkan dengan ukuran mata jaring 1 ¼ inchi. Komposisi jumlah jenis dan bobot udang pada ukuran mata jaring yang didapat berbeda nyata.  Kata Kunci: Tanjung Solok, Udang, Sondong, Ukuran mata jaring ABSTRACTTanjung Solok has estuary waters with a sloping beach base and has a muddy substrate which makes the Tanjung Solok area one of the centers for shrimp catching. One of the fishing gear used to catch shrimp in Tanjung Solok Village is the sondong fishing gear. This study aims to compare the catch and composition of shrimp using fishing gear of sondong with different mesh sizes in Tanjung Solok Village. This research was conducted on 24 November to 18 December 2020 at the Tanjung Solok Village, Kuala Jambi District. The research method used is a survey method. The technique of determining the location uses the purposive sampling method, while the respondent is taken from one fisherman each (1 inch mesh size is taken by one fisherman and the mesh size is 1 ¼ inch for one fisherman) with data frequency of 16 catches on each different mesh size. The results showed that the catch of sondong for 16 times the catch of 1 inch mesh obtained 36,166 fishes with a weight of 340.80 kg, while the size of the mesh 1 ¼ inch resulted in a catch of 28,795 fish with a weight of 271.10 kg. Based on this research, it can be neglected that the catch of shrimp using a fishing gear is 1 inch more than the mesh size of 1 ¼ inch. The composition of the number of species and the weight of shrimp in the mesh sizes obtained was significantly different. Keywords: Tanjung Solok, Shrimp, Sondong, Mesh Size
KUALITAS SEMEN BEKU KAMBING PERANAKAN ETAWAH PADA PERMUKAAN NITROGEN CAIR DENGAN JARAK YANG BERBEDA (Frozen semen quality of pe goat in liquid nitrogen surface with different distance) Revy Andryansyah; Teguh Sumarsono; Fachroerrozi Hoesni; Bayu Rosadi
JURNAL NUKLEUS PETERNAKAN Vol 7 No 1 (2020): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i1.2205

Abstract

The research was undertaken to study the effect of frozen semen distance from liquid nitrogen surface during handling on spermatozoa quality of PE goat. Thirty straws of PE goat frozen semen was allotted into six treatments i.e. T0 (control, straws submerged in liquid nitrogen), T1 to T5 based on distance of straws to liquid nitrogen surface were 5, 10, 15, 20, and 25 cm. Variables measured were motility, viability, and abnormality of spermatozoas. The results showed that PE goat frozen semen exposed in different distance to liquid nitrogen surface decreased (P<0.05) motily and viability of spermatozoas and had no effect (P>0.05) on abnormality of spermatozoas. The motily and viability was decreased in T4 and T5. In conlusion, exposing PE goat frozen semen from liquid nitrogen surface at 15 cm or less for 5 min maintain the quality of spermatozoas.
Co-Authors A. Parakkasi Ade Nopianti ADRIANI ADRIANI Afriani H Arief Boediono Bayu Islami Pasyah D Darmawan Darmawan Darmawan Depison Depison Dondin Sajuthi Endri Musnandar Engki Zelpina Enny T Setiatin Fachroerrozi Hoesni, Fachroerrozi Farizal Farizal Farizal Farizal Firmansyah Firmansyah Gunawan G Guspianto Guspianto Jalius Jalius Jodion Siburian Liber Hasudungan Sinurat Lusi Amidia M Agus Setiadi Mursalin Mursalin Mustakim Mustakim Nisfu Bayu Kurniadi Revy Andryansyah Silvia Erina Suhardi Pratama Sutrisno Sutrisno Syafril Hadi Taufiq Arrahman Teguh Sumarsono Thomas Mata Hine Trio Mohammad Syafi&#039;i