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Rini Widayanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Journal : Jurnal Veteriner

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Kajian Molekuler Daerah D-Loop Parsial DNA Mitokondria Kuda (Equus caballus) Asli Tengger Yuriadi -; Rini Widayanti; Aris Purwantoro; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tengger’s horse (Equus caballus) is a local Indonesian horse an originated from its ancestor in Java.As the population of Tengger’s horse is almost extinct it is important to conserve and increase the horsepopulation by in situ or ex situ conservation.The objective of this research was to study the moleculargenetic of partial D-loop of Tengger’s horse. Sequencing of PCR product, showed that the D-loop consistedof 319 nucleotides. The DNA was isolated from whole blood and amplified and sequenced using a publishedprimer sets. The sequence was aligned and compared with horse D-loop sequences available in Genebankusing Clustal W method in MEGA program version 4.0.2. Ten different nucleotide sites were found inTengger horse from (nucleotide no. 9, 52, 64, 69, 102, 117, 133, 170, 187 and 293). The genetic distanceanalised using Kimura 2-parameter model ranged between 0,0% and 3,2%, with the average of 1,7%. Thephylogenetic tree using neighbor joining method based on the sequence of nucleotide partial D-loop couldnot be used to differentiate among horse from Tengger and E. caballus.
Kekerabatan Genetik Caplak Rhiphicephalus (Boophilus) microplus Asal IndonesiaBerdasarkan Sekuen Internal Transcribed Spacer-2 (GENETIC RELATIONSHIP INDONESIAN RHIPHICEPHALUS (BOOPHILUS) MICROPLUS TICK BASED ON INTERNAL TRANSCRIBED SPACER-2 SEQUENSE ) Ana Sahara; Joko Prastowo; Rini Widayanti; Kurniasih .; Wisnu Nurcahyo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Rhiphicephalus (Boophilus) microplus is important obligatory blood feeding ectoparasites transmittingmany different viral, bacterial and protozoan and plays a role as a vector of Babesia sp., The leria sp. andAnaplasma sp. in cattle. The accuracy in identifying and distinguishing interspecies and intraspeciesdiversity among parasites is needed to understand the epidemiology, biology and capacity as a vector.Variations in the DNA base sequence of the internal transcribed spacer region2 (ITS 2) has been used asa molecular marker for identification in an effort to determine phylogenetic relationships. The aim of thisstudy was to determine the ITS 2 gene nucleotide sequence of R. microplus, which was expected to beuseful for accurate identification the parasite diversity and phylogenetic relationship among many differentspecies. DNA amplification was conducted using BOO2 forward dan BOO2 reverse primers. The DNAsamples containing ITS2 region fragment of 1099 nt were derived from the nucleotide sequence multiplealignments of R.microplus and other ticks genes obtained from Gene bank using Clustal W software, andthen analyzed using the MEGA program version 6. Genetic distances based on nucleotide sequence weredetermined with Kimura 2-parameter method producing the smallest genetic distance of 0 % and 1.2 %.Construction of phylogenetic trees using the Neighbor joining method showed that ticks from variousregions in Indonesia was species complex which have a closer with R.microplus isolates from India, Laos,South Africa, China and Australia R.australis origin.
Keragaman Genetik Gen Penyandi Dehydrogenase Sub-unit 3 Mitokondria pada Monyet Hantu (Tarsius sp.) (GENETIC DIVERSITY OF MITOCHONDRIAL DEHYDROGENASE SUB-UNIT 3 GENE ON TARSIUS SP.) Rini Widayanti; Niken Satuti Nur Handayani; I Made Budiarsa
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tarsius is an endemic species in Indonesia but is is getting threatened. In-situ and ex-situ conservationof this species would yield better results when its genetic information and diversity determined. Theobjective of this ressearch was to study the genetic diversity of ND3 gene of Tarsius sp. Based on sequencingof PCR products using primer ND3F and ND3R resulted in 437 nts base sequence. Results of ND3 fragmentssequencing were put on multiple alignment with other primates from Genbank using of software ClustalW, and then were analyzed using MEGA program version 4.1. A different nucleotide site was found(nucleotide no. 24). The genetic distance based on nucleotide ND3 was calculated using Kimura 2-parametermodel. The genetic distance showed the smallest genetic distance 0%, the biggest 0,03% and average0,01%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide ND3 genecould not be used to differentiate among T. Dianae (from Central Sulawesi), T. Spectrum (from NorthSulawesi) and T. bancanus (from Lampung, South Sumatera).
Identification Species of Myxobolus from Gill of Cyprinus carpio in East Java (IDENTIFIKASI MYXOBOLUS SP YANG DIPEROLEH DARI INSANG IKAN KARPER DI JAWA TIMUR) Agus Priyono; Kurniasih .; Rini Widayanti; Ayuda Dyah Nurekawati
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of study was to identify  Myxobolus sp. obtained from the gills of carp (Cyprinus carpio) ofEast Java, Indonesia. The cysts containing spores were collected from the gills of carp fish. The spores wereexamined by wet mounts preparation, fixed with ethanol absolute solution for molecular analysis. Thespores had a transparent membrane, the shell, composed of two valves. The sutural ridge running betweenthe valves. It was two anterior polar capsules, each consisted of a coiled polar filament. An iodinophilicvacuole and sporoplasm nuclei was located in posterior part. DNA Sequenses 18S rDNA followed byphylogenetic tree demonstrated that Myxobolus sp from Blitar was different from Myxosoma cerebralis ofthe Gene Bank. Myxosoma cerebralis was not found  in the fresh water fish in Indonesia.
Co-Authors Agus Priyono Amalia Rezeki Ana Sahara Aris Haryanto Aris Purwantoro Ayuda Dyah Nurekawati Charles Rangga Tabbu Dedi Duryadi Solihin Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Heri Budi Santoso Irma Padeta Joko Prastowo Kurniasih . Laksono Trisnantoro Niken Satuti Handayani Niken Satuti Nur Handayani TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Budipitojo Wayan Tunas Artama Widya Asmara Wisnu Nurcahyo Yuriadi - Yuriadi .