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Journal : Jurnal Natur Indonesia

 1 
Kemampuan Kitinase Streptomyces RKt5 sebagai Antijamur terhadap Patogen Fusarium oxysporum Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.537 KB) | DOI: 10.31258/jnat.14.1.42-46

Abstract

The purpose of the reasearch is to determine of antifungal activity from chitinase from Streptomyces RKt5 to inhibite growth of Fusariumoxysporum. The chitinase of Streptomyces RKt5 produced in liquid chitin medium with optimum conditions (inoculum concentration, pHand incubation time) and then partially purified with ammonium sulphate. The enzyme products were tested the antifungal activity againstF.oxysporum. The results showed that mycelial growth of F.oxysporum can be inhibited by Streptomyces RKt 5 in dual culture test. Thepartial purified chitinase enzyme couldn’t inhibit the fungal growth. But if the mycellium fragmented, the enzyme could degrade the fungalcell wall in incubation time. The frequency of fungal cell wall lysis and levels of N-acetylglucosamine released that have been increasingalong with the length of incubation time.
Co-Authors A.A. Ketut Agung Cahyawan W Ambarwati Artama, I Wayan Tunas Bambang Setiaji C. J. Soegihardjo Charlie Ester de Fretes, Charlie Ester Christina Salaki Dwi Suhartanti Elisa Nurnawati Endang Sutriswati Rahayu Helen Joan Lawalata Jamhari Jamhari Jesmandt Situmorang Jusup Subagja, Jusup Laksono Trisnantoro Lela Susilawati Lisa Lisdiana Masashi Kawaichi, Masashi Niken Handayani Nurhayani H. Muhiddin, Nurhayani H. Orryani Lambui S. Rahayu, S. Sebastian Margino Selvi Marcellia Sri Darmawati Subagja, Yusup Subagus Wahyuono Sukarti Moeljopawiro Tanti Azizah S Tri Ardyati Wayan T. Artama Wayan T. Artama Widayati, Wiwik E. Widya Asmara Yekti Asih Purwestri Yurnaliza, Yurnaliza