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All Journal Jurnal Teknik ITS Jurnal Sains dan Seni ITS IPTEK Journal of Proceedings Series IPTEK The Journal for Technology and Science Bioeksperimen: Jurnal Penelitian Biologi Akta Kimia Indonesia Jurnal Purifikasi Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Sewagati
Enny Zulaika
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Teknologi Sepuluh Nopember (ITS)
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Engineering Environmental Science Social Sciences

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Studi Literatur Potensi Bakteri Endogenik Lahan Gambut Sebagai Biofertilizer untuk Memperbaiki Nutrisi Lahan Hilda El Yasa Alam; Enny Zulaika
Jurnal Sains dan Seni ITS Vol 9, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23373520.v9i2.55624

Abstract

Lahan gambut merupakan lahan dengan kesuburan rendah sehingga kurang layak digunakan sebagai lahan pertanian. Rekayasa lahan gambut untuk meningkatkan kesuburan dapat dilakukan dengan bioaugmentasi bakteri endogenik yang bersifat lignoselulolitik, memiliki potensi sebagai biofertilizer dan menghasilkan hormon auksin. Uji sinergisme dilakukan pada Bacillus D1, D2, D3, U2, U4 dan Pseudomonas U3 menggunakan metode streak plate. Hasilnya tidak menunjukkan zona hambat pada persinggungan antar isolat. Beberapa bakteri yang diisolasi dari lahan gambut seperti genus Bacillus dan Pseudomonas mampu memproduksi enzim lignoselulolitik. Genus yang lain juga memiliki kemampuan menyediakan nitrat, melarutkan fosfat dan kalium serta memproduksi hormon Indole-3-Acetic Acid (IAA), sehingga berpotensi digunakan sebagai biofertilizer untuk memperbaiki nutrisi lahan gambut.
POTENSI Azotobacter spp. SEBAGAI PENDEGRADASI LIPID DAN PROTEIN Waritsatul Firdausi; Enny Zulaika
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 2: September 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v1i2.873

Abstract

Azotobacter spp. merupakan salah satu genus bakteri nonsimbiotik yang berperan penting dalam bidang agrikultur terkait kemampuannya memfiksasi nitrogen. Penelitian ini bertujuan untuk mengetahui potensi isolat Azotobacter dari lahan Eco Urban Farming ITS dalam mendegradasi lipid dan protein. Kemampuan isolat dalam mendegradasi lipid dan protein ditentukan dengan pengukuran Indeks Biodegradasi (IB). Isolat yang digunakan adalah A1a, A1b, A2, A3, A5, A6, A7, A8, A9, dan A10. Semua isolat mampu mendegradasi lipid kecuali A5, dengan IB tertinggi pada isolat A1b (1,00). Semua isolat dapat mendegradasi protein kecuali A7, dengan IB tertinggi terdapat pada isolat A8 (3,00).
Ligninolytic Enzymes Produced by Gliomastix sp. in an Organic Waste Medium Nengah Dwianita Kuswytasari; Maya Shovitri; Enny Zulaika
IPTEK The Journal for Technology and Science Vol 26, No 1 (2015)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v26i1.577

Abstract

Ligninolytic enzymes are extracelluler enzymes that have many benefit on multiple industrial department. Its can result from Gliomastix sp. This study was aimed to determine the effect of temperature and pH on the activity of Ligninolytic enzymes by Gliomastix sp. T3.7 that grow on multiple organic waste . Ligninolytic enzymes was measured with spectrofotometer UV-Vis Boeco S22. The result showed that Gliomastix sp. T3.7 potentially produce the ligninolytic enzymes like as laccase, LiP and MnP. The optimum laccase activity ( 1,765 U/ml) detected on sugarcane baggase waste, pH 6 and temperature of 350C . The optimum LiP activity (8,088U/ml) detected on corn cobs, pH 5 and 350C, whereas the optimum MnP activity (0,964 U/ml) detected on sugarcane baggase too. Its optimum on pH 4 and 250C. Gliomastix sp. T3.7 on sugarcane bagasse, pH 6 and 350C, agensia potentially used as remediation on a variety of industry pollution.
Extracellular Alkaline Phosphatase from Mangrove Soil Yeast Nur Hidayatul Alami; Windasari Putri Septarina; Tania Pratiwi; N. D Kuswytasari; Enny Zulaika; Maya Shovitri
Akta Kimia Indonesia Vol 4, No 1 (2019)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.135 KB) | DOI: 10.12962/j25493736.v4i1.5090

Abstract

Alkaline phosphatase is a widely used enzyme in research and industry. Yeast is one of a microbial group that has the potency to produce alkaline phosphatase. This study aims to screen yeast from  mangrove in Surabaya’s East Coast with the potential of alkaline phosphatase and to optimize the production of these enzymes. Screening test includes the measurement of phosphate solubilization index and concentration of soluble phosphate. Yeast with potential of phosphate solubilization will be used to measured the enzyme activity and also further optimized for extracellular alkaline phosphatase production. Optimization was done by determining the incubation time and a variety of combinations of phosphate source and temperature. The incubation time was adjusted to 7 days. Ca3(PO4)2 and KH2PO4 as the phosphate source and incubation temperature were adjusted to 29° C, 45° C, and 55° C. The results showed that isolates with the highest phosphate solubilization index were W1.1 and G3.2 (1.18 for W1.1 and 1.15 for G3.2). Yeast with the ability to solubilize phosphate was assumed to be Candida genus. W1.1 and G3.2 produced soluble phosphate in a concentration of  0.50 ppm and 0.77 ppm at the 7th day respectively. Candida G3.2 has higher enzyme activity than Candida W1.1. Candida G3.2 has optimal production for 3 days incubation time with a combination of temperature 29 °C and Ca3(PO4)2as the phosphate source which caused 97.8 U/mL enzyme activity.  
ENZIM NITRAT REDUKTASE SEBAGAI INDIKATOR KEBERHASILAN FITOREMEDIASI PADA LUMPUR SIDOARJO Enny Zulaika
Purifikasi Vol 14 No 2 (2014): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25983806.v14.i2.17

Abstract

Penelitian bertujuan untuk membuktikan keberhasilan fitoremediasi menggunakan Cassia fistula dengan indikator peningkatan aktivitas enzim nitrat reduktase di daun tanaman. Preparasi lumpur menggunakan penambahan pasir dan pupuk organik 1:1:1, 1:1:2, 1:1:3, 1:1:4. Pengukuran aktivitas nitrat reduktase menggunakan metode spektrofotometri. Substrat enzim terdiri dari buffer pospat 9,8 ml dan 0,2 ml 0,1 M NaNO3. Preparasi enzim menggunakan substrat 0,2 ml ditambah 2 ml reagen pewarna {1 ml 0,02% N-Naphtyl Ethylen Diamin (NED) dan 1 ml 0,1 % sulfanilamid dalam HCL 3 N}. Perubahan warna diukur dengan spektrofotometer UV pada panjang gelombang 540 nm. Peningkatan aktivitas enzim nitrat reduktase di daun tanaman C. fistula menunjukkan keberhasilan proses fitoremediasi terhadap lumpur Sidoarjo. Peningkatan ANR dari minggu ke 2 sampai 8 menunjukkan korelasi positip (R2 > 0,9). Modifikasi lumpur pada perlakuan V (L:P:PO = 1:1:3) merupakan ANR yang signifikan untuk indikator fitoremediasi, yaitu 0,0369 - 0,0503 µmolx10-3/mg/jam dari minggu ke 2 - 8.
VIABILITY AND PRODUCTION CALCIFYING BACTERIAL ENDOSPORE ON SAND-CEMENT CARRIER Apriliani Devinta; Enny Zulaika
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.184

Abstract

Carbonatogenic bacteria have the ability to precipitate calcium carbonate (CaCO3) and many calcareous areas are found. Some of its species formed endosporas which resistant to harsh physical condition such as very alkaline pH. The objectives of this study were to obtain spore biomass and determine endosporas viability in tested carrier media such as sand-cement. The tested isolates were Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, and Sporosracina JA4. The production of endosporas was carried out on yeast urea broth with a temperature treatment of 70°C for 20 minutes. The formed endosporas were stored in carrier medium of sand, cement, and a mixture of cement sand. Spore viability was conducted using total plate count method. The results showed that all isolates were able to produce endosporas with the highest endosporas dry biomass is Bacillus AK4 which was 196 mg/L. After 2 hours of storage, endosporas were still able to grow by forming colonies on nutrient agar media.
PRODUCTION OF AMYLOCELLULOLYTIC ENZYMES AND THEIR VIABILITY ON CARRIER MEDIA BY Bacillus sp. U4 AND Pseudomonas sp. U3 Enny Zulaika; Salma Shavira Rahma Khofifah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.186

Abstract

Amylase and cellulase are enzymes that are amyocellulolytic. Both are extracellular enzymes that can degrade organic materials, namely starch and cellulose. The purpose of this study was to determine whether Bacillus sp. U4 and Pseudomonas sp. U3 could produce amylocellulolytic enzymes and determine their viability after being incubated for 2 hours on the husk, peat, and sawdust carrier media. Screening for the presence of amylase was carried out on a selective medium, namely nutrient agar-amylum 0.5 %, and cellulase on carboxymethyl cellulose-agar medium. Both isolates were inoculated into sawdust, husk, peat carrier media and incubated for 2 hours. Viability was observed using the total plate count method. Bacillus sp. U4 and Pseudomonas sp. U3 can produce amylase and cellulase. The best isolate viability after incubation for 2 hours was found in husk carrier media with 2.13 x 105 CFU/gr of husk media.
BIOACCUMULATION OF IRON (Fe) IN Bacillus JA1, Sporosarcina JA4, AND Lysinibacillus JB2 Afianita Dian Rahmawati; Enny Zulaika
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.188

Abstract

Carbonatogenic bacteria are able to produce calcium carbonate (CaCO3). Steel reinforcement of concrete is mainly made of a mixture of carbon (C) and ferrous (Fe) elements. Fe is classified as heavy metal, when in high concentrations it is toxic to bacteria. The purpose of this study was to determine the bioaccumulation of carbonatogenic bacteria to Fe. The isolates used in this study were Bacillus JA1, Sporosarcina JA4, and Lysinibacillus JB2. All isolates were subcultured on nutrient agar slant media. Cultures were made on minimal salt medium: nutrient broth (95% : 5%) with 24 hours incubation. Bioaccumulation test using minimal salt medium: nutrient broth (95% : 5%) containing ferrous 5 ppm and 10 ppm. Accumulated ferrous concentration was measured using atomic absorption spectrophotometry. The result of this research is that the bioaccumulation of ferrous by the isolates after 2 hours of incubation is greater than 1 hour of incubation. Bacillus JA1 has the highest bioaccumulation ability which was 9,19 ppm after 2 hours exposure of 10 ppm ferrous and its ferrous bioaccumulation efficiency was 91,85%.
Greenhouse Potential based on Ecotourism and Education for Sustainable Village Economic Resilience Lilik Nurindah Sari; Tutik Nurhidayati; Maya Shovitri; Enny Zulaika; Nengah Dwianita Kuswytasari; Arif Luqman; Nur Hidayatul Alami; Kristanti Indah Purwani; Nurul Jadid; Dini Ermavitalini; Imam Wahyudi Farid; Ciptian Weried Priananda
IPTEK Journal of Proceedings Series No 1 (2023): The 1st International Conference on Community Services and Public Policy (ICCSP) 2022
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2023i1.16378

Abstract

Indonesia has many rural areas with diversity and uniqueness in each region that have developed into eco-tourism. The village that is being developed as an eco-tourism destination is expected to improve the welfare of the surrounding community. Developed eco-tourism can provide jobs for residents in the village. Eco-tourism is also one of the developments to preserve ecosystems in rural areas. This eco- tourism sector can support community welfare and sustainable rural development. One approach to the development of rural areas is through village ecotourism. Village Ecotourism is a rural area with several special characteristics that can be used as a tourist destination. One way to improve the development of village ecotourism is by adding new facilities that lead to educational tourism, i.e., greenhouse facilities. The existence of this greenhouse can be used as a means of science education about a more advanced agricultural system with a controlled environmental system. Greenhouse technology can improve the quality and quantity of plant productivity, thereby increasing people's income, and producing healthier organic plant products. Greenhouses can also be used as educational tourism facilities for various science education activities and simple agricultural training. Training for residents can also be carried out, for community empowerment, such as training in planting, fertilizing, nurseries, processing plant products, and the process of packaging plant products. This review summarizes the various potentials of Greenhouse development for the development of education-based village ecotourism and provides references for further research that focuses on community service, which is increasing sustainable village economic resilience.
Kolaborasi Institusi Pemerintah – Perguruan Tinggi – LSM dalam Pengelolaan Sampah Plastik Melalui Forum Group Discussion Aunurohim; Anies Wijayanti; Hermawan; Enny Zulaika; Dian Saptarini; Dewi Hidayati; Maya Shovitri; Edwin Setiawan; Farid Kamal Muzaki; Iska Desmawati; Nova Maulidina Ashuri
Sewagati Vol 3 No 2 (2019)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1276.813 KB)

Abstract

Pengelolaan sampah, utamanya plastik, menjadi sorotan Indonesia bahkan dunia terkait dengan pencemaran yang disebabkannya. Pengelolaan sampah plastik menjadi penting untuk dilakukan manakala sudah mencapai taraf yang membahayakan makhluk hidup di perairan ataupun di daratan. Departemen Biologi bersama Dinas Lingkungan Hidup kota Surabaya dan Lembaga Swadaya Masyarakat Komunitas ‘Nol’ Sampah Surabaya mengadakan forum group discussion guna membahas hal tersebut agar diperoleh suatu upaya konkret dalam pengelolaan sampah plastik di Surabaya secara khusus, dan Indonesia secara umum. Hasil forum group discussion kegiatan ini merekomendasikan langkah-langkah konkret berdasarkan prioritas dalam pengelolaan sampah plastik dimulai dari (1) tidak menggunakan sama sekali tas atau kantong plastik, sedotan plastik, dan botol minuman plastik sekali pakai, (2) jika tidak memungkinkan, maka dilakukan pengurangan penggunaan material berbahan dasar plastik sekali pakai, (3) skala prioritas selanjutnya adalah melakukan re-cycle ataupun re-use untuk plastik yang tidak bernilai komersil seperti plastik sachet ataupun bungkus mie agar dapat bernilai fungsional bahkan komersial, (4) dan prioritas terakhir adalah membiasakan membuang sampah terutama plastik pada tempatnya agar mekanisme sortikasi yang dilakukan pemerintah untuk melaksanakan program bebas sampah plastik pada tahun 2025 mendatang dapat terwujud.
Co-Authors Adisya Prima Nurmalita Sari Afianita Dian Rahmawati Afriha Mafazah Anies Wijayanti Anindya Citra Asri Anjar Lulu Sakinah Apriliani Devinta Arif Luqman Arzulinda Maulidar Rahmasyitha Aunurohim Aunurohim Avip Nur Fitria Ciptian Weried Priananda Denaya Andrya Prasidya Dewi Hidayati Dini Ermavitalini Djoko Purwanto Edwin Setiawan Fahima Tahta Kholida Farid Kamal Muzaki Febriana Puji Rahayu Gian Rukmana Hermawan Hilda El Yasa Alam Idea Rahmania Machsun Imam Wahyudi Farid Indah Budi Asih Iska Desmawati Kristanti Indah Purwani Kusnul Khotimah Kuswytasari Kuswytasari Kuswytasari, Nengah Dwianita Laily Hanifa Lericka Mei Permadi Lilik Nurindah Sari Maya Shovitri N. D Kuswytasari Nadia Ulfiyati Nitra Wahyuningsih Nova Maulidina Ashuri Nur Hidayatul Alami Nurul Jadid Prajna Wirya Kencana Putra Rachma Shouma Annisa Rahma Nadia Maudy Rindan Novanti Riska Irawan Rosidah Kumalasari Ryan Widi Anggar Kusuma Salma Shavira Rahma Khofifah Saptarini, Dian Septa Tri Farisna Sri Fatmawati Tania Pratiwi Trio Verdian Tutik Nurhidayati Wahyu Dewi Iftita Waritsatul Firdausi Waritsatul Firdausi Waritsatul Firdausi Windasari Putri Septarina Wirdatul Muslihatin