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All Journal HAYATI Journal of Biosciences Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner ENGINEERING JUPE The Indonesian Journal of Public Administration (IJPA) Istinbath: Jurnal Hukum dan Ekonomi Islam RELIGIA JURNAL HUKUM ISLAM Ovozoa: Journal of Animal Reproduction Millah: Journal of Religious Studies Religia : Jurnal Ilmu-Ilmu Keislaman

Imam Mustofa

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Author-ID : 453865

Religion Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Law, Crime, Criminology & Criminal Justice Social Sciences Veterinary Other

Published : 27 Documents Claim Missing Document

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Pengimbuhan Minyak Jagung pada Pakan Meningkatkan Hirarki Folikuler dan Produktivitas Burung Puyuh (CORN OIL SUPLEMENTATION IN FEED INCREASE FOLICULLAR HIERARCHY AND PRODUCTIVITY OF JAPANESE QUAIL) Herinda Pertiwi; Imam Mustofa; Tatik Hernawati
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to measure the effect of corn oil in feed on estrogen hormone level in blood, hierarchy follicular performance and productivity of Japanese quail. Thirty female Japanese quail were housed in caged and fed ad libitum, the birds were randomly divided into three grouped namely: Po, P1 and the (P0) was fed with commercial feed without corn oil, whereas the first test treatment (P1) was fed with commercial feed mixture containing 3% corn oil, and the second test treatment (P2) was feed with commercial feed mixture containing 6% corn oil along the study. Every day the egg production was recorded. After eight weeks, blood samples were collected from jugular vein to isolate the serum. Then all samples were sacrificed to observe the hierarchy follicular performance. Based on this study it was found that 3% and 6 % corn oil supplementation on feed (P1 and P2) did not cause any significant changes in serum estrogen concentration, and productivity, but had significant changes on hierarchy follicular. This result show that hierarchy follicular increasing due to ovarian activity optimizing induced by polyunsaturated fatty acid (PUFA)by consumed corn oil. Thus, the suplementation of corn oil can be an alternative to increase egg productivity of quail farm. ABSTRAK Penelitian ini bertujuan untuk mengukur pengaruh pemberian minyak jagung dalam pakan terhadap kadar estrogen serum darah, hirarki folikular dan produktivitas burung puyuh. Sebanyak 30 ekor burung puyuh betina dibagi menjadi tiga perlakuan. Perlakuan kontrol diberi pakan komersial tanpa suplementasi minyak jagung, sedangkan perlakuan P1 dan P2 diberi pakan komersial dengan suplementasi minyak jagung sebanyak 3% dan 6%. Setiap hari dilakukan pencatatan terhadap produktivitas. Setelah delapan minggu, dilakukan koleksi sampel darah, kemudian semua burung puyuh dikorbankan nyawanya untuk mengamati hirarki folikular yang terbentuk. Pada P1 dan P2 tidak telihat perbedaan yang nyata terhadap peningkatan kadar estrogen serum darah dan produktivitas dibandingkan dengan P0, akan tetapi terlihat perbedaan nyata pada hirarki folikular yang terbentuk. Hasil ini menunjukkan bahwa peningkatan hirarki folikular yang terbentuk terjadi karena peningkatan kinerja ovarium dipicu oleh polyunsaturated fatty acid (PUFA) yang berasal konsumsi minyak jagung. Dengan demikian, suplementasi minyak jagung dapat dijadikan alternatif untuk meningkatkan produksi telur dalam peternakan burung puyuh.

Aluminosilikat Berpotensi Menekan Gangguan Reproduksi Mikotoksin Zearalenon Berdasarkan Pengamatan Jumlah Folikel dan Ekspresi Caspase-9 Ovarium Muhammad Thohawi Elziyad Purnama; Imam Mustofa; Tri Wahyu Suprayogi; Abdul Samik; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Zearalenone is a resorcylic acid lactone produced by fungal Fusarium graminearum in contaminated edible grains and can cause reproduction disorder in animals by binding to estrogen receptors on target cells. The aim of this study was to assess the potential use of aluminosilicates as mycotoxin binders to eliminate the adverse effect of zearalenone by examining the number of follicles and caspase-9 expression in the ovary of mice. The study adopted a completely randomized simple design using 20 mices which were randomly divided into five group each of which consisted of four mices. Five treatment groups consisted of K+ (without zearalenone and aluminosilicates); K- (treated with zearalenone 0.1 mg/mice/day); P1 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 0.5 mg/mice/day); P2 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 1 mg/mice/day); and P3 were treated with zearalenone 0,1 mg/mice/day and aluminosilicates 2 mg/mice/day) with gastric tube daily for 10 days. The data obtained from this study were analyzed by analysis of variance and proceeded with Duncan test. The result showed that the primary follicles, secondary follicles, tertiary follicles and de Graaf follicles increased significantly on P3 treatment group. Caspase-9 expressions decreased significantly in all of aluminosilicates groups as compared to positive control. The treatment of mice with zearalenone and aluminosilicates increases the number of follicles and decreased caspase-9 expression in the ovary of mice. ABSTRAK Zearalenon merupakan senyawa resorcylic acid lactone yang diproduksi oleh jamur Fusarium graminearum dan dapat mengakibatkan gangguan reproduksi pada ternak dengan membentuk ikatan pada reseptor estrogen. Penelitian ini bertujuan untuk menguji potensi aluminosilikat terhadap mencit yang telah dipapar zearalenon pada aspek jumlah folikel dan ekspresi caspase-9 organ ovarium. Penelitian ini menggunakan 20 ekor mencit yang dibagi menjadi lima kelompok perlakuan dengan masing-masing empat ulangan, yakni K+ tanpa dipapar zearalenon dan aluminosilikat; K- dosis zearalenon 0,1 mg/ekor/hari; P1 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 0,5 mg/ekor/hari; P2 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 1 mg/ekor/hari; P3 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 2 mg/ekor/hari dengan sonde lambung selama sepuluh hari. Data hasil skoring dan perhitungan dianalisis dengan sidik ragam dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa jumlah folikel primer, sekunder, tersier dan de Graaf pada P3 terjadi peningkatan yang signifikan. Ekspresi caspase-9 ovarium menunjukkan penurunan pada semua perlakuan aluminosilikat. Simpulan penelitian ini adalah mencit yang dipapar zearalenon dan kemudian diberi aluminosilikat mengalami peningkatan jumlah folikel dan penurunan ekspresi caspase-9 pada organ ovarium.

Potensi Protein Reseptor Fertilisasi Zona Pelusida Kambing Sebagai Kandidat Imunokontrasepsi dengan Fertilisasi in vitro pada Sapi (POTENTIAL OF FERTILIZATION RECEPTOR PROTEIN GOAT ZONA PELLUCIDA AS CANDIDATE OF IMMUNOCONTRACEPTION BY USING IN VITRO FER Sri Mulyati; Imam Mustofa; Laba Mahaputra
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Studies on the role of zona pellucida glycoprotein-3 (ZP3) in immunocontraception have been conductedin many species including goats (gZP3). Our previous study indicated that gZP3 effective in preventingpregnancy in mice. The aim of this study was to prove the evidence of cross reaction in gZP3 to preventfertilization in cow in vitro. Antibody of gZP3 was produced in mice. Immunized mice serum was analyzedusing ELISA technique and dot blot method. Sperm from frozen semen were processed then incubated incapacitation media supplemented with the gZP3, whilst the cow oocyte was incubated in maturationmedia supplemented with antibody of gZP3. Following this, the normal in vitro fertilization (withoutincubation neither in gZP3 nor in gZP3 antibody) was performed, respectively. The results showed thatantibody titer of immunized mice serum was higher (p<0.05) than the control group. Dot blotting analysisshowed that the antibody of immunized mice were able to recognize gZP3 protein. The serum of immunizedmice which was supplemented in the maturation media of cow oocyte were able to decrease the cleavagerate (p<0.05). Protein gZP3 which was supplemented in the capacitation media of the sperm also coulddecrease the cleavage rate (p<0.05). It is concluded that goat-ZP3 protein may produce cross reaction incow.

Co-Authors Abdul Rosid Ma'ruf Abdul Samik Ade Jubaedi Aghid Cahya P. Ajis S Adang Djaha Amung Logam Saputro Arimbi Ashabul Yamin Aucky Hinting Bambang Sektiari Lukiswanto Benjamin Chr Tehupuring BUDI UTOMO Dyah Ayu Roro Risna Y ERMA SAFITRI Faiz Muhamad azhari Fajar Septian H Fedik Abdul Rantam Fitry Rahmawaty Frans Nurseto Hana Eliyani Hanif Sabekti Pratama Hardijanto Hardijanto Herinda Pertiwi Hermin Ratnani Herry Agoes Hermadi Ismudiono Ismudiono Laba Mahaputra Lutfi Ardiana Melkisedek N.B.C. Neolaka Melsa Netika Muhammad Thohawi Elziyad Purnama Muhammad Yunus Mujahid Mujahid Pudji Srianto Ragil Angga Prastiya Ratna Damayanti Rendy Kurnia Dewanta Rimayanti Rimayanti Roesno Darsono Rosiana Febrianila Sony Susanto Sri Mulyati Sri Mulyati Suwarno . Suwarno Suwarno Suzanita Utama Tatik Hernawati Tjuk Imam R. Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Widjiati . Widjiati Widjiati Widya Aulia Ristiani Widya Paramita L. Widya Paramita Lokapirnasari Yoes Priyatna Dachlan

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