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Sekuensing 16S DNA Bakteri Selulolitik Asal Limbah Cairan Rumen Sapi Peranakan Ongole (SEQUENCING OF 16S DNA OF CELLULOLYTIC BACTERIA FROM BOVINE RUMEN FLUID WASTE ONGOLE CROSSBREED) Widya Paramita Lokapirnasari; Adriana Monica Sahidu; Tri Nurhajati; Koesnoto Supranianondo; Andreas Berny Yulianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.233 KB) | DOI: 10.19087/jveteriner.2017.18.1.76

Abstract

This study aimed to identified cellulolytic inoculant code WPL 214 isolated from bovine rumen fluid waste of Ongole Cross Breed of Surabaya Slaughter house. A single colony of isolates celulolytic grown on 5 mL of liquid media Luria Bertani (LB) consist of 1 % NaCl , 1% tripton , 0.5 % yeast extract, containing1 % carboxymethyl cellulose (CMC) at temperature 37°C, using a shaker of incubator during 16-18 hours. That isolate determined by 16S DNA gen analysis using High Fidelity Platinum Taq DNA Polymerase with primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ and primer reverse PB38 5’-GMT ACCTTG TTA CGA CTT-3’ for PCR. Nucleotide sequence of 16S DNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. DNA isolation and 16S DNA coding genes amplification were carried out using Kit High Fidelity Platinum Taq DNA Polymerase. Afterward, BLAST was applied to identify the phylogenetic tree. The bacteria was capable of indicating the existence of clear zone in a media CMC by congo red staining. The existence of the clear zone associated with the activity of microbes to degrade cellulose. The conclusión of this research based on the results was the sequencing nucleotides genome 16S DNA showed that cellulolytic inoculant was identified as Enterobacter cloacae WPL 214. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi lebih lanjut isolat selulolitik kode WPL 214 yang telah diisolasi dari cairan rumen sapi peranakan ongole dari limbah Rumah Potong Hewan Surabaya. Koloni tunggal dari isolat selulolitik ditumbuhkan pada 5 mL media cair Luria Bertani (LB) dengan komposisisi 1% NaCl, 1% tripton, 0,5% yeast ekstrak, yang mengandung 1% substrat carboxymethyl cellulose (CMC) pada suhu 37°C, dengan pengocokan menggunakan shaker incubator selama ±16-18 jam. Penelitian ini terdiri dari dua tahap, tahap pertama dilakukan isolasi DNA, tahap kedua dilakukan identifikasi gen penyandi 16S DNA, amplifikasi DNA dengan polymerase chain reaction (PCR). Amplifikasi gen penyandi 16S DNA menggunakan Kit High Fidelity Platinum Taq DNA Polymerase dengan primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ dan primer reverse PB38 5’-GMT ACC TTG TTA CGA CTT-3’ yang digunakan untuk PCR. Hasil sekuensing nukleotida dari 16S DNA selanjutnya dibandingkan dengan urutan nukleotida dari GenBank database untuk dilakukan BLAST untuk mengidentifikasi berdasarkan pohon filogeni. Bakteri tersebut mampu menunjukkan adanya zona bening pada media Carboxymethyl cellulose (CMC) dengan pewarnaan congo red. Adanya zona bening tersebut berhubungan dengan aktivitas mikrob untuk mendegradasi selulosa. Simpulan penelitian menunjukkan bahwa berdasarkan hasil urutan nukleotida genom 16S DNA serta pohon filogeni, maka isolat selulolitik tersebut diidentifikasi sebagai Enterobacter cloacae WPL 214.
Pengaruh Subtitusi Tepung Biji Bunga Matahari (Helianthus Annuus L.) dalam Pakan Komersial dengan Konsentrasi Tertentu Terhadap Performa Ayam Pedaging Beta Hermansyah; Widya Paramita Lokapirnasari
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.682 KB) | DOI: 10.20473/jmv.vol2.iss1.2019.7-12

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggantian tepung biji bunga matahari (Helianthus annuus L.) di pakan komersial dengan konsentrasi tertentu pada kinerja ayam pedaging. Penelitian ini menggunakan desain acak lengkap. Sampel terdiri dari 20 ekor ayam broiler dibagi secara acak menjadi 5 kelompok. Perlakuan adalah P1, P2, P3, dan P4 masing-masing CP511 pakan komersial yang mengandung tepung biji bunga matahari 25%, 30%, 35%, dan 40% serta kontrol tanpa substitusi P0 tepung biji bunga matahari. Variabel yang digunakan adalah konsumsi pakan, bobot akhir dan konversi pakan kemudian hasilnya dianalisis dengan Analisis Varians. Hasil yang diperoleh rata-rata nilai konsumsi pakan P0 adalah 79,76 gram, P1 adalah 77,90 gram, P2 adalah 77,35 gram, P3 adalah 77,98 gram, P4 adalah 77,58 gram. Hasil nilai rata-rata berat akhir 1545,5 gram yaitu P0, P1 adalah 1296,5 gram, P2 1325,75 gram, P3 1256 gram, P4 1203 gram. Hasil yang diperoleh dari nilai rata-rata konversi pakan adalah P0 1,37, P1 1,69, P2 1,61, P3 1,71, P4 1,88. Berdasarkan hasil dapat disimpulkan bahwa substitusi tepung biji bunga matahari dalam diet komersial tidak meningkatkan asupan pakan, berat badan dan dapat meningkatkan konversi pakan.
POTENSI KOMBINASI BAKTERI DAN JAMUR SELULOLITIK PADA FERMENTASI BEKATUL TERHADAP KANDUNGAN SERAT KASAR DAN PROTEIN KASAR Widya Paramita Lokapirnasari; Agus Setiawan; Soetji Prawesthirini
Buletin Peternakan Vol 39, No 3 (2015): BULETIN PETERNAKAN VOL. 39 (3) OKTOBER 2015
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v39i3.7985

Abstract

PENAMBAHAN PROBIOTIK LACTOBACILLUS ACIDOPHILUS DAN BIFIDOBACTERIUM SP TERHADAP ANALISIS USAHA AYAM PETELUR YANG DIINFEKSI ESCHERICHIA COLI Khoirul Huda; Widya Paramita Lokapirnasari; Soeharsono Soeharsono
Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis Vol 5, No 2 (2019): Juli 2019
Publisher : Universitas Galuh Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.019 KB) | DOI: 10.25157/ma.v5i2.1931

Abstract

The purpose of this study was to determine the use of probiotics Lactobacillus acidophilus and Bifidobacterium sp on variable cost, income and profit layers hens was infected with Escherichia coli. About 120 layers at 25 weeks of age were randomized into six treatments. The treatment consisted of factors a (infection and non-infection Escherichia coli) and factor b (basal feed, AGP and probiotics). The results of the study gave probiotics an effect (p <0.05) on variable cost, income and profit. Infection factors have an effect (p <0.05) on income and profit, but have no effect (p> 0.05) on variable cost. There was interaction between probiotic and infection factors (p <0.05) on income and profit, but there was no interaction (p> 0.05) between probiotic and infection factors on variable cost. The highest variable cost was found in the treatment of non-infectious probiotics is Rp 13.594, the highest income was treated with non-infectious probiotics is Rp 26.564, the highest profit was treated with non-infectious probiotics is Rp 12.969. It could be concluded that the administration of probiotics can produce good production performance.
The Effect of Additional Feed Fermentation of Moringa Oleifera Leaves on The Cholesterol Level of Mojosari Laying Ducks Ratna Dwi Lestari; Widya Paramita Lokapirnasari; Muhammad Anam Al Arif; Sri Hidanah; Soeharsono Soeharsono; Mirni Lamid
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.221-225

Abstract

The purpose of this research was to determine the effect of supplementary feeding with fermented Moringa leaf flour on cholesterol levels of Mojosari duck eggs and to calculate the cost of production. This study used 60 mojosari ducks, layer phase aged 27 weeks. Moringa flour was fermented using probiotics and given in four treatments, namely P0 (100% commercial feed), P1 (0.5% moringa leaf flour fermentation + 100% commercial feed), P2 (1% moringa leaf flour fermentation + 100% commercial feed), P3 (1.5% fermented Moringa leaf meal + 100% commercial feed). The variable observed in this study was cholesterol levels in Mojosari duck eggs. The results showed thatgiving Moringa leaf flour fermentation can give significantly different values (p <0.05) in each treatment both P0, P1, P2 and P3.In the fermentation of moringa leaf flour as much as 1.5% (P3) had the lowest cholesterol levels (113.13 ± 12.55) Meanwhile, 1% (P2) of moringa leaf flour fermentation has the highest cholesterol level (170.42 ± 13.19).
Effect of Probiotics Bacillus subtilis on Feed Efficiency and Egg Mass of Laying Hens Hanif Sabekti Pratama; Widya Paramita Lokapirnasari; Soeharsono Soeharsono; Muhammad Anam Al-Arif; Nenny Harijani; Sri Hidanah
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.37-41

Abstract

The aim of this research was to investigate the effect of probiotics Bacillus subtilis on feed efficiency and egg mass of laying hens. This research was using 36 of 38 weeks-old laying hens Isa Brown strain. The concentration of probiotics B. subtilis was 1 x 109 CFU/ml, there were three kinds of treatments, P0 (without given probiotics B. subtilis), P1 (given 0.1 gram of probiotics B. subtilis per kg of feed), and P2 (given 0.2 gram of probiotic B. subtilis per kg of feed). The results of feed efficiency, the provision of probiotics can provide a significantly different values (p <0.05) between treatments either P0, P1, or P2. Provision probiotics P1 showed the highest value of feed efficiency (81.72 ± 5.28), while the lowest value at P0 was (64.06 ± 3.30). The results showed that there were significantly different in egg mass values (p <0.05) between treatments both P0, P1, and P2. Probiotic P2 shows the highest value (53.30 ± 2.13), while the lowest egg mass value of probiotics is as much as P1 (40.83 ± 4.73). Based on the results of this study it can be concluded that the B. subtilis probiotics both at a dose of 0.1 gram / kg of feed and a dose of 0.2 gram / kg of feed can affect the growth performance of laying hens.
EFEK PENGGUNAAN PROBIOTIK Lactobacillus casei DAN Bifidobacterium sp TERHADAP ANGKA KONVERSI PAKAN DAN KONSUMSI PAKAN PADA AYAM PETELUR YANG DIINFEKSI Escherichia coli Nabil Fariz Noorrahman; Widya Paramita Lokapirnasari; Mohammad Anam Al arif; Koesnoto Soepranianondo; Sri Hidanah; Sarmanu Sarmanu
BRILIANT: Jurnal Riset dan Konseptual Vol 4, No 2 (2019): Volume 4 Nomor 2, Mei 2019
Publisher : Universitas Nahdlatul Ulama Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.997 KB) | DOI: 10.28926/briliant.v4i2.288

Abstract

Penelitian ini bertujuan mengetahui probiotik Lactobacillus casei dan Bifidobacterium sp terhadap rasio konversi pakan dan konsumsi pakan pada ayam petelur yang diinfeksi escherichia coli. Dalam penelitian ini menggunakan sebanyak 120 ekor ayam petelur umur 25 minggu dengan  menggunakan metode percobaan desain Rancangan Acak Lengkap (RAL) pola faktorial. Probiotik L.casei 108 CFU/ml dan Bifidobacterium sp 108 CFU/ml diberikan seacara oral melalui air minum sebanyak 250 ml air/ekor/hari. Hasil penelitian efek probiotik Lactobacillus casei dan Bifidobacterium (p <0,05) konsumsi pakan tidak menunjukkan perbedaan yang nyata (p>0,05). Konsumsi pakan tidak menunjukkan perbedaan antara perlakuan dan kontrol yaitu berkisar 113,72 sampai 114,22  g/ekor/hari. Konversi pakan menunjukkan perbedaan yang nyata p<0,05 terhadap FCR. Terdapat interaksi antara infeksi dengan feed additive p<0,05 terhadap FCR.
PENGGUNAAN PROBIOTIK KOMBINASI lactococcus lactis DAN L actobacillus acidophilus SEBAGAI PENGGANTI ANTIBIOTIKA PADA AYAM PETELUR YANG DIINFEKSI E scherichia coli TERHADAP ANALISIS USAHA Hana Cipka Pramuda Wardhani; Widya Paramita Lokapirnasari; Koesnoto Soepranianondo
BUANA SAINS Vol 19, No 1 (2019)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.484 KB) | DOI: 10.33366/bs.v19i1.1527

Abstract

Escherichia coli is a normal flora in the digestive system of laying hens that are non-pathogenic, which can change into pathogens and cause the egg production to decrease. So the combination of Lactococcus lactis and Lactobacillus acidophilus probiotics is expected to be able to overcome E. coli and become a substitute for the antibiotics (Virginiamycin) in animal feed. This study aims to determine business analysis including Break Event Points (BEP), Revenue Cost Ratio (R / C Ratio), Payback Period (PP) and Return On Investment (ROI). The best results obtained for the calculation of Break Event Point (BEP) on a0b2 treatment with a BEP of Rp. 17,587,24 with BEP production on a1b2 of 14,36 kg, Revenue Cost Ratio (R / C Ratio) generates a value of 1,543 for treatment a0b2, Payback Period (PP) generates a value of 1 year 3 months 9 days and Return On Investment (ROI) generates a value of 3. It was concluded that the a0b2 treatment had good results to be developed.
Probiotic Potential of Lactic Acid Bacteria on Feed Efficiency, Weight and Carcass Percentage in Ducks Evania Haris Chandra; Widya Paramita Lokapirnasari; Sri Hidanah; Muhammad Anam Al-Arif; Wiwik Misaco Yuniarti; Epy Muhammad Luqman
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.69-73

Abstract

The aim of the study was to determine the effect of the use of probiotic lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) on feed efficiency, carcass weight and carcass percentage in broiler ducks. Probiotics lactic acid bacteria produce bacteriocins and organic acids that inhibit the growth of pathogenic microbes in the digestive tract of poultry, so that the intestinal villi of broiler ducks are healthy. Besides that, probiotics are able to reduce cholesterol levels in meat when consumed by humans and can improve health. This study used 48 peking ducks. The probiotics of Lactic Acid Bacteria used were a concentration of 1.2 x 108 CFU/ml. In this study there were four different treatments, namely P0 (without probiotics), P1 (given 1 ml probiotics/liter drinking water), P2 (given 2 ml probiotics/liter drinking water) and P3 (given 3 ml probiotics/liter of drinking water). The results of the feed efficiency study showed that there was a significant difference (p<0.05) in the P0, P1, P2 and P3 treatments. The highest feed efficiency was at P3 (33.81%) while the lowest was P0 (30.84%). The results of the study on carcass weight indicated that the probiotics tended to be higher than those who were not given probiotics but statistically not significantly different (p>0.05). The highest carcass weight was P3 (1749.92 g) and the lowest was P0 (1709.25 g). The results of the study on the percentage of carcass weight showed a significant difference (p<0.05) in both P0, P1, P2 and P3 treatments. The highest percentage of carcass weight was at P3 (64.60%) and the lowest was P0 (60.96%). It can be concluded that the administration of probiotics lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) at a dose of 1 ml/liter of drinking water, 2 ml/liter of drinking water and 3 ml/liter of drinking water can affect feed efficiency and the percentage of duck carcasses. but has no effect on the carcass weight of broiler ducks.
Potensi Bakteri Lactococcus sp. dan Lactobacillus sp. untuk Peningkatan Kualitas Limbah Kulit Kacang Sebagai Alternatif Bahan Pakan [Potential of Lactococcus sp. and Lactobacillus sp. Bacteria for Quality Improvement of Peanut Peel Waste as Alternative Feed Ingredients] Widya Paramita Lokapirnasari; Oky Setyo Widodo; Emy Koestanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 10 No. 1 (2018): Jurnal ilmiah perikanan dan kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.226 KB) | DOI: 10.20473/jipk.v10i1.8547

Abstract

                                                    AbstrakTujuan penelitian ini untuk menterminasi peningkatan kandungan nutrien dalam fermentasi makanan yang terdapat dari kulit kacang sebagai bahan pakan alternatif untuk ternak dan ikan. Penelitian ini terdiri dari tiga perlakuan dan lima replikasi P0: kulit kacang+isolat 0%; P1: kulit kacang+1% Lactococus sp. ; P2 : kulit kacang+1% Lactobacillus sp. Proses fermentasi dilakukan selama 5 hari dalam keadaan aerob. Hasil menunjukan bahwa terdapat berdedaan yang signifikan antara perlakuan (p<0,05). Penggunaan isolat Lactococcus sp. dan Lactobacillus sp. dapat meningkatkan kandungan nutrient dari limbah kulit kanang. Terdapat penurunan aerob mentah dari 3,80 sampai 7,70% terdapat peningkatan elestrak nitrogen bebas dari 3,925 sampai 4,38% terdapat peningkatan kandungan energi pencernaan 7.13% sampai 9.30%. berdasarkan hasil penelitian dapat disimpulkan bahwa pengguna 1% Lactococcus sp. dan 1% Lactococcus sp. dapat meningkatkan nilai nutrisi di limbah kulit kacang yang dapat digunakan sebagai alternatif bahan pakan ternak dan ikan.                                                   AbstractThis study aims to determine the increasing of nutrient content in the fermentation of feed stuff derived from peanut wastes as an alternative feed material for livestock and fish. The study consisted of three treatments and five replications, namely P0: peanut peel + 0% isolate, P1: peanut peel + 1% Lactococcus sp, P2: peanut peel +1% Lactobacillus sp. The fermentation process was carried out for 5 days under a facultative anaerobic condition. The results showed that there was a significant difference between treatments (p <0.05). The use of Lactococcus sp and Lactobacillus sp isolates can increase the nutritional content of peanut peel wastes. There was a decrease in crude fiber content (CF) of 3.80 to 7.70%, there was an increase of Nitrogen free extract (NFE) of 3.925 to 4.38%, there is an increase in digestible energy (DE) content of 7.13% to 9.30%, there was the increasing metabolizable energy (ME) of 7.13% to 9.12% compared to control. Based on the results it can be concluded that the using of 1% Lactococcus sp and 1% Lactobacillus sp. can increase nutritional value of peanut peel waste that can be used as an alternative ingredients of animal and fish.
Co-Authors AGUS SETIAWAN Agustono - Agustono Agustono Amung Logam Saputro Anam Al Arif Andreas Berny Yulianto Anita Dwi Andriani Annise Proboningrat Aprinda Ratna Lovela Ardianto Ardianto Ayu Aprodhita Cintya Bellani Balqis Karimah Beta Hermansyah Bodhi Agustono Chairul Anwar Nidom Dimas Wicaksana Elok Sukma Pertiwi Emy Koestanti Emy Koestanti Sabdoningrum Epy Muhammad Luqman Epy Muhammad Luqman ERMA SAFITRI Evania Haris Chandra Faisal Fikri Fithria Nisa Hanifah Gogik Satrio Margo Utomo Hanif Sabekti Pratama Hanif Sabekti Pratama Hermin Ratnani I. Ismudiono Imam Mustofa Imam Mustofa Irmaya Triasih Iwan Sahrial Hamid Karlina Hardianing Pangestu Kautsar Pandu Pramudita Kemala Hudita Khasmawati Mas'ad Khoirul Huda Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Supranianondo Koesnoto Suprianondo Kusnoto Kusnoto Laili Salisa Masruroh Lilik Maslachah M. Anam Al Arif M. Anam Al-Arif M. Gandul Atik Yuliani Mariana Ruth Theresia Hutabarat Mas'ud Hariadi Maya Nurwartanti Yunita Mirni Lamid Moh. Anam Al Arif Moh. Anam Al-Arief Mohammad Anam Al Arif Mohammad Anam Al-Arif Mohammad Sukmanadi Muhammad Anam Al Arif Muhammad Anam Al Arif Muhammad Anam Al-Arif Muhammad Anam Al-Arif Muhammad Anam Al-Arif Muhammad Arief Nabil Fariz Noorrahman Nabil Fariz Noorrahman Nanik Hidayatik Nenny Harijani Nenny Harijani Nenny Harijani Ninda Rubi Pramestya Nurul Ika Wardiana Oky Setyo Widodo Pudji Srianto Putri Indra Irawan Ragil Angga Prastiya Ragil Angga Prastiya Rahardian Wicaksono Ratna Damayanti Ratna Dwi Lestari Retno Sri Wahjuni Rifqi Najwan Rifqi Najwan Rimayanti Rimayanti Rini Fajarwati Rochmah Kurnijasanti Sahidu, Adriana Monica Sarmanu Sarmanu Siti Hadijah Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soetji Prawesthirini Sri Hidanah Sri Pantja Madyawati Suheni Susilowati Suzanita Utama TARZAN PURNOMO Tjuk Imam Restiadi Tri Nurhajati Tri Nurhajati Tri Wahyu Suprayogi Wardhani, Hana Cipka Pramuda Warsito, Sunaryo Hadi Wiwik Misaco Yuniarti