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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) JITEK (Jurnal Ilmiah Teknosains) Briliant: Jurnal Riset dan Konseptual Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis Jurnal Medik Veteriner BUANA SAINS Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Journal of Marine and Coastal Science Bantara Journal of Animal Science Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Global Pharma Technology Journal of Fisheries & Marine

Widya Paramita Lokapirnasari

Divisi Peternakan, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 2896422

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Veterinary Other

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EFEK PENGGUNAAN PROBIOTIK Lactobacillus casei DAN Bifidobacterium sp TERHADAP ANGKA KONVERSI PAKAN DAN KONSUMSI PAKAN PADA AYAM PETELUR YANG DIINFEKSI Escherichia coli Nabil Fariz Noorrahman; Widya Paramita Lokapirnasari; Mohammad Anam Al arif; Koesnoto Soepranianondo; Sri Hidanah; Sarmanu Sarmanu
BRILIANT: Jurnal Riset dan Konseptual Vol 4, No 2 (2019): Volume 4 Nomor 2, Mei 2019
Publisher : Universitas Nahdlatul Ulama Blitar

Penelitian ini bertujuan mengetahui probiotik Lactobacillus casei dan Bifidobacterium sp terhadap rasio konversi pakan dan konsumsi pakan pada ayam petelur yang diinfeksi escherichia coli. Dalam penelitian ini menggunakan sebanyak 120 ekor ayam petelur umur 25 minggu dengan menggunakan metode percobaan desain Rancangan Acak Lengkap (RAL) pola faktorial. Probiotik L.casei 108 CFU/ml dan Bifidobacterium sp 108 CFU/ml diberikan seacara oral melalui air minum sebanyak 250 ml air/ekor/hari. Hasil penelitian efek probiotik Lactobacillus casei dan Bifidobacterium (p <0,05) konsumsi pakan tidak menunjukkan perbedaan yang nyata (p>0,05). Konsumsi pakan tidak menunjukkan perbedaan antara perlakuan dan kontrol yaitu berkisar 113,72 sampai 114,22 g/ekor/hari. Konversi pakan menunjukkan perbedaan yang nyata p<0,05 terhadap FCR. Terdapat interaksi antara infeksi dengan feed additive p<0,05 terhadap FCR.

Performa Produksi dan Analisis Usaha Puyuh (Coturnix coturnix japonica) yang diberi Substitusi Black Soldier Fly Larvae (BSFL) pada Pakan Komersil Fithria Nisa Hanifah; Koesnoto Soepranianondo; Soeharsono Soeharsono; Anam Al Arif; Widya Paramita Lokapirnasari; Nenny Harijani; Siti Hadijah; Mariana Ruth Theresia Hutabarat
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Black Soldier Fly Larvae (BSFL) is an alternative protein source for livestock. This research aimed to know the effectiveness of BSFL for commercial feed substitution, related to production, productivity, and profitability in quail farm. This study used 80 quails which divided into 4 groups and each group consist of 20 quails. The treatments contained BSFL substitution with different composition, control group (P0) was given 100% commercial feed, (P1) was given a 5% BSFL substitution, (P2) was given a 10% BSFL substitution and (P3) was given a 20% BSFL substitution. Contribution margin (CM) analysis was used to determine the yields of the groups in this study. Results showed that the highest feed consumption rate is in P3, production (egg in total and weight), productivity and yields in P3 is the highest, although there's no difference between P0, P2 and P3 based on the analysis MANOVA. BSFL substitution at 20% is recommended to the farmers to increase profits.

POTENSI KOMBINASI BAKTERI DAN JAMUR SELULOLITIK PADA FERMENTASI BEKATUL TERHADAP KANDUNGAN SERAT KASAR DAN PROTEIN KASAR Widya Paramita Lokapirnasari; Agus Setiawan; Soetji Prawesthirini
Buletin Peternakan Vol 39, No 3 (2015): BULETIN PETERNAKAN VOL. 39 (3) OKTOBER 2015
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v39i3.7985

Uji Aktivitas Pertumbuhan Enterobacter cloacae Selulolitik Aerob Rumen-1 Isolat Asal Limbah Cairan Rumen Sapi Peranakan Ongole (GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAE SAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CRO Tri Nurhajati; Koesnoto Soepranianondo; Widya Paramita Lokapirnasari
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.273 KB)

This study aimedto know the growth activityofcellulolytic bacteria EnterobactercloacaeSAR 1isolatedfrombovinerumen fluidwaste. Isolates that had been cultured were taken as much as 10 mL and thentransferred to100 mL growth medium in Erlenmeyer flask. Culturesuspensions were incubated in a shakerincubator (37°C, 120 rpm). Optical density was measured at ë 600 nm by taking as much as 1 mLsampling with interval of two hours for 24 hours (hour 0; 2; 4; 6; 10; 12; 14; 16; 18; 20; 22; 24). The firstsampling was done at 0thhour and continued until OD values †showed a clear decline. Optical density wasmeasured with a UV-Vis spectrophotometer at wave length ë 600 nm. Growth curve was obtained from theresult of absorbance measurement on the time. Optimum growth production of E.cloacae SAR 1 occurredat the 12thhoursof incubation, optimum temperature of 35°C and optimum pH 6.

Sekuensing 16S DNA Bakteri Selulolitik Asal Limbah Cairan Rumen Sapi Peranakan Ongole (SEQUENCING OF 16S DNA OF CELLULOLYTIC BACTERIA FROM BOVINE RUMEN FLUID WASTE ONGOLE CROSSBREED) Widya Paramita Lokapirnasari; Adriana Monica Sahidu; Tri Nurhajati; Koesnoto Supranianondo; Andreas Berny Yulianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aimed to identified cellulolytic inoculant code WPL 214 isolated from bovine rumen fluid waste of Ongole Cross Breed of Surabaya Slaughter house. A single colony of isolates celulolytic grown on 5 mL of liquid media Luria Bertani (LB) consist of 1 % NaCl , 1% tripton , 0.5 % yeast extract, containing1 % carboxymethyl cellulose (CMC) at temperature 37°C, using a shaker of incubator during 16-18 hours. That isolate determined by 16S DNA gen analysis using High Fidelity Platinum Taq DNA Polymerase with primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ and primer reverse PB38 5’-GMT ACCTTG TTA CGA CTT-3’ for PCR. Nucleotide sequence of 16S DNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. DNA isolation and 16S DNA coding genes amplification were carried out using Kit High Fidelity Platinum Taq DNA Polymerase. Afterward, BLAST was applied to identify the phylogenetic tree. The bacteria was capable of indicating the existence of clear zone in a media CMC by congo red staining. The existence of the clear zone associated with the activity of microbes to degrade cellulose. The conclusión of this research based on the results was the sequencing nucleotides genome 16S DNA showed that cellulolytic inoculant was identified as Enterobacter cloacae WPL 214. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi lebih lanjut isolat selulolitik kode WPL 214 yang telah diisolasi dari cairan rumen sapi peranakan ongole dari limbah Rumah Potong Hewan Surabaya. Koloni tunggal dari isolat selulolitik ditumbuhkan pada 5 mL media cair Luria Bertani (LB) dengan komposisisi 1% NaCl, 1% tripton, 0,5% yeast ekstrak, yang mengandung 1% substrat carboxymethyl cellulose (CMC) pada suhu 37°C, dengan pengocokan menggunakan shaker incubator selama ±16-18 jam. Penelitian ini terdiri dari dua tahap, tahap pertama dilakukan isolasi DNA, tahap kedua dilakukan identifikasi gen penyandi 16S DNA, amplifikasi DNA dengan polymerase chain reaction (PCR). Amplifikasi gen penyandi 16S DNA menggunakan Kit High Fidelity Platinum Taq DNA Polymerase dengan primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ dan primer reverse PB38 5’-GMT ACC TTG TTA CGA CTT-3’ yang digunakan untuk PCR. Hasil sekuensing nukleotida dari 16S DNA selanjutnya dibandingkan dengan urutan nukleotida dari GenBank database untuk dilakukan BLAST untuk mengidentifikasi berdasarkan pohon filogeni. Bakteri tersebut mampu menunjukkan adanya zona bening pada media Carboxymethyl cellulose (CMC) dengan pewarnaan congo red. Adanya zona bening tersebut berhubungan dengan aktivitas mikrob untuk mendegradasi selulosa. Simpulan penelitian menunjukkan bahwa berdasarkan hasil urutan nukleotida genom 16S DNA serta pohon filogeni, maka isolat selulolitik tersebut diidentifikasi sebagai Enterobacter cloacae WPL 214.

Pemberdayaan Masyarakat Melalui Aplikasi Teknologi Inseminasi Buatan, Pengolahan Pakan , Biofarmaka dan Limbah dalam Upaya Pengembangan Sentra Kambing Lilik Maslachah; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 2 (2019): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

ABSTRACT Community empowerment of the goat farming group in Kerek and Merakurak, Tuban aims to improve the knowledge of artificial insemination technology of goats, processing agricultural and plantation waste products for goat feed,processing and using medicinal plants and processing goat’s faeces waste to become environmentally-friendly fertilizer. Methods: observing the location, interviewing and discussing with the leader of the group to clarify the problems faced by goat farmers. Education given by seminar and training by demos of artificial insemination, complete feed processing, bio-pharmaceutical preparation and waste processing. Evaluation and monitoring the success of the sustainability program cooperating with local animal husbandry department for assistance by field operators serving on the area. The output of TTG is transformation of artificial insemination technology of goats. Knowledge and understanding of farmers about how to process complete feed for goats. Making bio pharmaceutical preparation independently. Production of environmentally-friendly fertilizer. Keywords: artificial insemination; bio-pharmaceutical; complete feed; waste product

PENGARUH PEMBERIAN PROBIOTIK Lactobacillus acidophilus DAN Bifidobacte-rium sp TERHADAP PERFORMA PRODUKSI AYAM PETELUR YANG DIINFEKSI Escherichia coli Khoirul Huda; Widya Paramita Lokapirnasari; Soeharsono Soeharsono
Jurnal Ilmiah Teknosains Vol 5, No 1 (2019): JiTek
Publisher : Universitas PGRI Semarang

The purpose of this study is to determine the use of probiotics Lactobacillus acidophilus and Bifidobacterium sp on feed consumption and Hen Day Production (HDP) laying hens which infected by Escherichia coli. Arround 120 layers at the age of 25 weeks are divided into six treatments. Each treatment consisted of factor a (infection and non-infection Escherichia coli) and factor b (basal feed, AGP and probiotics). The result of this study shows that giving probiotic has taken an effect (p<0,05) on the feed consumption and HDP. The infection factor takes effect (p <0.05) on HDP but it does not take effect (p>0,05) on the feed consumption. There is interaction among probiotic factor and infection (p>0.05) on HDP but it has no effect (p> 0.05) on feed consumption. The highest feed consumption is found in the giving of non-infectious probiotics that is 114.7 g and the highest HDP which is from the giving of non-infectious probiotics is 98.6 %. In conclusion, the giving of probiotic Lactobacillus acidophilus and Bifidobacterium sp are able to produce well perform product and the high profit.

PENGARUH PENAMBAHAN PROBIOTIK LACTOBACILLUS ACIDOPHILUS DAN BIFIDOBACTERIUM TERHADAP PRODUKSI AYAM PETELUR YANG DIINFEKSI ESCHERICHIA COLI Rifqi Najwan; Widya Paramita Lokapirnasari; Soeharsosno soeharsono; Khoirul Huda
BRILIANT: Jurnal Riset dan Konseptual Vol 4, No 2 (2019): Volume 4 Nomor 2, Mei 2019
Publisher : Universitas Nahdlatul Ulama Blitar

Tujuan dari penelitian ini adalah untuk mengetahui penggunaan probiotik Lactobacillus acidophilus dan Bifidobacterium sp terhadap Feed Conversion Ratio (FCR) dan berat telur ayam petelur yang diinfeksi Escherichia coli. Sebanyak 120 ekor ayam petelur umur 25 minggu diacak menjadi 6 perlakuan, masing-masing perlakuan terdiri dari 4 ulangan dan setiap ulangan terdiri dari 5 ekor ayam petelur. perlakuan terdiri dari faktor a (infeksi dan non infeksi Escherichia coli) dan factor b (pakan basal, AGP dan probiotik). Hasil penelitian Pemberian probiotik berpengaruh (p<0,05) terhadap FCR dan berat telur. Faktor infeksi berpengaruh (p<0,05) terhadap FCR dan berat telur. Terdapat interaksi antara faktor probiotik dan infeksi (p<0,05) terhadap FCR dan berat telur.

PENAMBAHAN PROBIOTIK LACTOBACILLUS ACIDOPHILUS DAN BIFIDOBACTERIUM SP TERHADAP ANALISIS USAHA AYAM PETELUR YANG DIINFEKSI ESCHERICHIA COLI Khoirul Huda; Widya Paramita Lokapirnasari; Soeharsono Soeharsono
Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis Vol 5, No 2 (2019): Juli 2019
Publisher : Universitas Galuh Ciamis

The purpose of this study was to determine the use of probiotics Lactobacillus acidophilus and Bifidobacterium sp on variable cost, income and profit layers hens was infected with Escherichia coli. About 120 layers at 25 weeks of age were randomized into six treatments. The treatment consisted of factors a (infection and non-infection Escherichia coli) and factor b (basal feed, AGP and probiotics). The results of the study gave probiotics an effect (p <0.05) on variable cost, income and profit. Infection factors have an effect (p <0.05) on income and profit, but have no effect (p> 0.05) on variable cost. There was interaction between probiotic and infection factors (p <0.05) on income and profit, but there was no interaction (p> 0.05) between probiotic and infection factors on variable cost. The highest variable cost was found in the treatment of non-infectious probiotics is Rp 13.594, the highest income was treated with non-infectious probiotics is Rp 26.564, the highest profit was treated with non-infectious probiotics is Rp 12.969. It could be concluded that the administration of probiotics can produce good production performance.

PENGGUNAAN PROBIOTIK KOMBINASI Lactococcus lactis DAN Lactobacillus acidophilus SEBAGAI PENGGANTI ANTIBIOTIKA PADA AYAM PETELUR YANG DIINFEKSI Escherichia coli TERHADAP ANALISIS USAHA Hana Cipka Pramuda Wardhani; Widya Paramita Lokapirnasari; Koesnoto Soepranianondo
Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis Vol 5, No 2 (2019): Juli 2019
Publisher : Universitas Galuh Ciamis

Escherichia coli is a normal flora in the digestive system of laying hens that are non-pathogenic, which can change into pathogens and cause the egg production to decrease. So the combination of Lactococcus lactis and Lactobacillus acidophilus probiotics is expected to be able to overcome E. coli and become a substitute for the antibiotics (Virginiamycin) in animal feed. This study aims to determine business analysis including Break Event Points (BEP), Revenue Cost Ratio (R / C Ratio), Payback Period (PP) and Return On Investment (ROI). The best results obtained for the calculation of Break Event Point (BEP) on a0b2 treatment with a BEP of Rp. 17,587,24 with BEP production on a1b2 of 14,36 kg, Revenue Cost Ratio (R / C Ratio) generates a value of 1,543 for treatment a0b2, Payback Period (PP) generates a value of 1 year 3 months 9 days and Return On Investment (ROI) generates a value of 3. It was concluded that the a0b2 treatment had good results to be developed.

Co-Authors Adriana Monica Sahidu Agustono - Agustono Agustono Amung Logam Saputro Anam Al Arif Andreas Berny Yulianto Anita Dwi Andriani Annise Proboningrat Aprinda Ratna Lovela Ardianto Ardianto Ayu Aprodhita Cintya Bellani Balqis Karimah Beta Hermansyah Bodhi Agustono Chairul Anwar Nidom Dimas Wicaksana Elok Sukma Pertiwi Emy Koestanti Emy Koestanti Sabdoningrum Epy Muhammad Luqman ERMA SAFITRI Evania Haris Chandra Faisal Fikri Fithria Nisa Hanifah Gandul Atik Yuliani Gogik Satrio Margo Utomo Hanif Sabekti Pratama Hanif Sabekti Pratama Hermin Ratnani I. Ismudiono Imam Mustofa Imam Mustofa Irmaya Triasih Iwan Sahrial Hamid Karlina Hardianing Pangestu Kautsar Pandu Pramudita Kemala Hudita Khasmawati Mas'ad Khoirul Huda Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Soepranianondo Koesnoto Supranianondo Koesnoto Suprianondo Kusnoto Kusnoto Laili Salisa Masruroh Laksono Trisnantoro Lilik Maslachah M. Anam Al Arif M. Anam Al-Arif Mariana Ruth Theresia Hutabarat Mas’ud Hariadi Maya Nurwartanti Yunita Mirni Lamid Moh. Anam Al Arif Moh. Anam Al-Arief Mohammad Anam Al-Arif Mohammad Sukmanadi Muchammad Achsinul Fikri Ma'ruf Muhammad Anam Al Arif Muhammad Anam Al Arif Muhammad Anam Al-Arif Muhammad Anam Al-Arif Muhammad Anam Al-Arif Muhammad Arief Nabil Fariz Noorrahman Nanik Hidayatik Nenny Harijani Nenny Harijani Nenny Harijani Ninda Rubi Pramestya Nurul Ika Wardiana Oky Setyo Widodo Pudji Srianto Putri Indra Irawan Ragil Angga Prastiya Ragil Angga Prastiya Rahardian Wicaksono Ratna Damayanti Ratna Dwi Lestari Retno Sri Wahjuni Rifqi Najwan Rifqi Najwan Rimayanti Rimayanti Rini Fajarwati Rochmah Kurnijasanti Sarmanu Sarmanu Siti Hadijah Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soeharsono Soetji Prawesthirini Sri Hidanah Sri Pantja Madyawati Suheni Susilowati Suzanita Utama TARZAN PURNOMO Tjuk Imam Restiadi Tri Nurhajati Tri Nurhajati Tri Wahyu Suprayogi Wardhani, Hana Cipka Pramuda Warsito, Sunaryo Hadi Wiwik Misaco Yuniarti

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