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The Relation between Malondialdehyde (MDA) and Histopatological Appearance in male Wistar Rats Model Fridayanti, Febrina Sylva; Sulistyaningsih, Erma; Sakinah, Elly Nurus
Journal of Agromedicine and Medical Sciences Vol 1, No 1 (2015)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Abstract

Fractures are a serious health problem in Indonesia due to increasing prevalence. The healing process of fracture is disturbed by the oxidative stress that caused by imbalance quantity of free radical and antioxidant. An antioxidant such as polyphenol, which can be found in cocoa, is needed to suppress oxidative stress. The study aimed to investigate the effect of the ethanolic extract of cacao on fracture healing process in a rat model through MDA concentration and histopatological appearance. This study is in vivo experimental study with post-test only controlled group design. 30 male Wistar rats were randomized and divided into 5 groups. 1 group was rats without fractured. The negative control and three treatment groups were rats with fractured manually on left tibia under anesthesia and immobilized by bandage. The treatment groups treated with cocoa ethanolic extract in a dose of 125 mg/kgBW, 250 mg/kgBW, and 500 mg/kgBW orally for 21 days. The result showed that there was a significant different between the treatment groups and the negative control group on MDA concentration and histopatological appearance (p>0,05). The corelation between them were strong and had negative direction (R=-0,771). The study concluded that cocoa ethanolic extract had a positive effect to supress oxidation stress and increases the number of osteoblast on fracture healing process.   Key words: cocoa ethanolic extract, polyphenol, fracture healing process, oxidative stress
The Effect Of Cholecalciferol On Fasting Blood Glucose In Streptozotocin-induced Hyperglycemia Mice Sakinah, Elly Nurus
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Glucose uptake into skeletal muscle cells require insulin-dependent and insulin independent signaling pathways, both leading to the translocation of glucose transporter-4 (GLUT4) to the plasma membrane. Insulin resistance occurs due to failure of insulin signaling to translocate GLUT4 resulting in the failure of glucose uptake and causing hyperglycemia. Cholecalciferol is known to have a function in regulating calcium homeostasis was shown to increase the synthesis of insulin and increasing insulin sensitivity. The purpose of this study is to explain the role of cholecalciferol to decreased fasting blood glucose in streptozotocin-induced hyperglyemia mice. 30 mice adapted for one week and then induced using 150mg/kgBW streptozotocin (STZ) intraperitoneally, After experiencing hyperglycemia mice were divided into 5 groups (n=6 each), Group I (hyperglycemic control), group II (25ng cholecalciferol), group III (50ng cholecalciferol), group IV (100ng cholecalciferol), and group V (metformin 300mg/kgBBB). Cholecalciferol given orally for 14th days. On day 15th the examination of fasting blood sugar levels were taken and the mice. Fasting blood sugar levels measured using a glucometer. Based on statistical analysis showed that there were significant differences in fasting blood sugar levels between treatment groups (p<0,001). Based on univariate regression analyses there was negative correlation of cholecalciferol with fasting blood glucose (p<0,001). Cholecalciferol may lower fasting blood sugar levels in hyperglycemia mice models. Keywords: cholecalciferol, fasting blood glucose, STZ.
Analog Rice Reduces Weight and Total Cholesterol Levels in Overweight and Hypercholesterolemic Rats Dewantari, Septiarina Putri; Hairrudin, Hairrudin; Sakinah, Elly Nurus
Jurnal Kedokteran Brawijaya Vol 31, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2020.031.01.2

Abstract

Overweight, whose prevalence is increasing, is caused by unbalanced consumption patterns such as high consumption of fat and can trigger hypercholesterolemia. Analog rice (AR), which has better nutritional content than ordinary rice, is thought to have beneficial effects on health. This study aimed to determine the potential of analog rice in reducing body weight and total cholesterol levels. A total of 24 male Wistar rats were divided into four groups, namely: I) control, II) ordinary rice treatment, III) analog rice 1 treatment (PBA1) and IV) analog rice 2 treatment (PBA2). The control group was given standard feed for 40 days ad libitum. Groups II, III, and IV were given a combination induction of high-fat diet (HFD) for 40 days ad libitum and intraperitoneal injection of streptozotocin (STZ) 35 mg/kg single dose on day 33. All groups were then given the treatment of diet for three weeks according to their respective groups, namely the standard, ordinary rice, analog rice 1, and analog rice 2 feed. Body weight and total cholesterol levels were measured in the pre-induction, post-induction/pre-treatment (pre-test), and post-treatment (post-test). The results of the study showed that the induction of the HFD-STZ combination carried out resulted in a significant increase in body weight and total cholesterol levels. Measurements made after dietary therapy showed that body weight and total cholesterol levels in the I and II groups did not change significantly, whereas those in the III and IV groups decreased significantly (p<0.05). This study concluded that the administration of analog rice could reduce body weight and total cholesterol levels in overweight and hypercholesterolemia rats. 
Aktivitas Hepatoprotektor Cuka Apel 'A' terhadap Kadar SGOT dan SGPT Serum Tikus Wistar yang Diinduksi Parasetamol Dosis Toksik (Hepatoprotector Activities of 'A' Apple Vinegar to SGOT and SGPT Serum in Wistar Rats Induced by Toxic Dose of Paracetamol) Fawziyah Putri Maulida; Hairrudin Hairrudin; Elly Nurus Sakinah
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

The use of a toxic dose of paracetamol is able to generate free radicals in the form of N-acetyl-p-benzoquinoneimine (NAPQI) resulting in increased levels of hepatic transaminase enzymes, Serum glutamate oksaloasetat transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT). 'A' apple vinegar contains antioxidants such as polyphenols, and vitamin C as well as acetic acid that were thought to have hepatoprotector effect which expected to prevent liver damage caused by NAPQI. The purpose of this study was to determine hepatoprotective activity of 'A' apple vinegar to SGOT and SGPT serum levels in wistar that induced by toxic dose of paracetamol. This study used 27 wistars wich were divided into three groups. The results of this study showed that average values of SGOT/SGPT in the normal control group were 79.00/113.89, negative control group were 289.67/296.22, treatment group were 180/194.44. Statistical test performed by using One Way Anova test for SGOT data and Kruskal Wallis test for SGPT. From the results it can be concluded that the administration of 'A' apple vinegar has hepatoprotective activity to SGOT and SGPT serum levels in wistar rats induced by toxic dose of paracetamol. Keywords: Apple vinegar, hepatoprotector, antioxidant, Paracetamol, Transaminase
Efek Hepatoprotektif Ekstrak Etanol Brokoli (Brassica oleracea L. var. italica) terhadap Kadar Malondialdehid Hepar Tikus Wistar yang Diinduksi DMBA (Hepatoprotective Effect of Ethanolic Extract of Broccoli (Brassica oleracea L. var. italica) on Liver Ma Dear Farah Sielma; Elly Nurus Sakinah; Yudha Nurdian
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

DMBA (7,12-Dimethylbenz(α)antracene) is a prototype of Polycyclic Aromatic Hydrocarbons (PAHs) which is present in automobile exhaust, cigarette smoke, and wood stoves. DMBA is converted into its reactive metabolites DMBA-DE and cause liver cell membrane lipid peroxidation, which results in an increased of liver malondialdehyde (MDA) levels. Broccoli contains flavonoid that able to prevent lipid peroxidation. The current study was conducted to find out the different hepatoprotective effect of ethanolic extract of broccoli against DMBA-induced hepatotoxicity in wistar rats using liver MDA as a marker. A total of 24 male wistar rats were divided into six groups, consisting of normal control (distilled water), negative control (DMBA), and treatment (ethanolic extract of broccoli in doses of 250, 500, 1000, and 2000 mg/kgBW). Rats were treated for 7 days prior to a single dose of DMBA on the 8th day of treatment. Liver tissue samples were taken on the 12th day for measurement of liver MDA levels. Data were analyzed using One Way ANOVA followed by Post Hoc Test LSD. The present study showed significant differences on liver MDA levels between groups (p<0.001). This study concluded that there were different hepatoprotective effects of ethanolic extract of broccoli treatment on liver MDA levels in wistar rats induced by DMBA. Keywords: ethanolic extract of broccoli, DMBA, malondialdehyde, hepatoprotective
Efek Pati Resisten Singkong (Manihot esculenta Crantz) terhadap Indeks HOMA-IR dan HOMA-B Tikus Model Diabetes Maria Ulfa; Elly Nurus Sakinah; Yohanes Sudarmanto; Jauhar Firdaus
Pustaka Kesehatan Vol 7 No 1 (2019): Volume 7 No. 1, 2019
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v7i1.17586

Abstract

Insulin resistance is a condition in which target organs fail to respond normally to insulin. Cassava contains 9,69 percent starch that resist to digestive enzyme in intestine and has been shown to increase insulin sensitivity and enhance β cell function. The aim of this study was to analyze the difference effect of native cassava starch and cassava resistant starch consumption on insulin resistance and β cell function indexes. This study was quasy experimental research with post-test only control group design using 16 male wistar strain rats divided into four groups: K1 (normal rats with standard diet), K2 (diabetic rats with standard diet), P1 (diabetic rats with native cassava starch diet) and P2 (diabetic rats with cassava resistant starch diet). The rats were fed with high fat diet and injected with low-dose streptozotocin to mimic the patogenesis of type 2 diabetes. The rats were then fed with assigned diet for 28 days. The data collection was obtained from laboratory examination of fasting plasma glucose and fasting plasma insulin to determine HOMA-IR and HOMA-B indexes. Post Hoc analysis showed no difference in both indexes between native cassava starch and cassava resistant starch fed groups (p>0,05).
Efek Hepatoprotektif Ekstrak Etanol Brokoli (Brassica oleracea L. var. italica) terhadap Kerusakan Histologis Sel Hati Tikus Wistar yang Diinduksi DMBA (Hepatoprotective Effect of Ethanolic Extract of Broccoli (Brassica oleracea L. var. italica) on the Histologically Damage of Wistar Rats Liver Cell Induced by DMBA) Muhtar Ady Kusuma; Elly Nurus Sakinah; Rosita Dewi
Pustaka Kesehatan Vol 5 No 1 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

DMBA (7,12-Dimethylbenz(α)antracene) is one of Polycyclic Aromatic Hydrocarbon’s form that is abundant on air pollution, i.e. automobile exhaust, cigarette smoke, and wood stoves. DMBA is metabolized in liver result in DMBA-DE. DMBA-DE is a free radical compound that has hepatotoxic effect. Broccoli contains flavonoid that has antioxidant effect. It can stabilize DMBA-DE radical compound. This research was conducted to investigate the hepatoprotective effect of ethanolic extract of broccoli on the damage of rat’s liver cells induced by DMBA. This research used 30 white male rats that was divided into 6 groups: normal control (aquadest), positive control (DMBA) and the treatment groups(using brocolli ethanolic extract with dose : 250, 500, 1000, and 2000 mg/kgBB). The treatment was given for 7 days followed by single-dose DMBA 15 mg/kgBB induction in the 8th day. Histological appearance of liver cells were examined on the 12th day. Data were analyzed by using Kruskal Wallis test followed by Mann Whitney. The result showed that there was a significant difference among the groups with p<0.05. In conclusion, there were some differences of hepatoprotective effect of broccoli ethanolic extract on the histologically damage of rats liver cells induced by DMBA. Keywords: ethanolic extract of broccoli, DMBA, histopathology, hepatoprotective
Pengaruh Pemberian Ekstrak Etanol Brokoli (Brassica oleracea) terhadap Kadar SGOT dan SGPT Tikus Wistar yang Diinduksi DMBA (The Effect of Ethanolic Extract of Broccoli (Brassica oleracea) on SGOT and SGPT of Wistar Rats Induced by DMBA) Rizka Nuzula Wardani; Elly Nurus Sakinah; Yudha Nurdian
Pustaka Kesehatan Vol 4 No 2 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

DMBA is a product of incomplete burning which can cause free radicals in human. The metabolism of DMBA by Cytochrome P450 in liver causes hepatocyte DNA damage and decrease endogenous antioxidant. Broccoli contains flavonoid which can act as an antioxidant by inhibiting formation of free radicals through hydrogen atom donation to free radicals. This research aimed to analyze the effect of ethanolic extract of broccoli on SGOT and SGPT of wistar rats induced by DMBA. This research used 24 rats divided into six groups: control group, negative control group, and four treatment groups with broccoli ethanolic extract of 250 mg/kgBW, 500 mg/kgBW, 1000 mg/kgBW, and 2000 mg/kgBW for 7 days. The SGOT and SGPT levels of control group were 70,36 U/L and 33,97 U/L; negative control group 107,16 U/L and 56,21 U/L; the first treatment 101,50 U/L and 49,33 U/L; the second treatment 85,32 U/L and 43,67 U/L; the third treatment 84,11 U/L and 40,84 U/L; and the fourth treatment 81,28 U/L and 35,18 U/L. The result of One Way Annova test for SGOT was p=0,012 while SGPT was p=0,003. In this study, ethanolic extract of broccoli could protect hepatocyte by decreasing SGOT and SGPT levels.   Keywords: ethanolic extract of broccoli, SGOT and SGPT levels, DMBA
Efek Ekstrak Etanol Kayu Manis (Cinnamomum burmannii) terhadap Kadar MDA Ginjal pada Tikus Wistar Hiperglikemi (The Effect of Cinnamon (Cinnamomum burmannii) Ethanolic Extract on Kidney MDA in Hyperglycemic Wistar Rats) Vidya Muqsita; Elly Nurus Sakinah; Ali Santosa
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Cinnamon contains natural antioxidant polyphenols. Polyphenols reduces lipid peroxidation by lowering levels of MDA. This study aimed to prove the effect of ethanolic extract of cinnamon (Cinnamomum burmannii) on decreasing kidney MDA levels of rat. This research was true experimental post-test only control group design. Twenty five rats 2-3 months of age, were divided into 5 groups: K(-) without alloxan induction, K(+) was induced with alloxan 100 mg/kg, group P1, P2, and P3 were induced with alloxan 100 mg/kg and cinnamon ethanolic extract 200 mg/kg, 400 mg/kg and 600 mg/kg, respectively for 7 days. At the end of the treatment, MDA concentration of kidney was measured by using ELISA method. The average of kidney MDA level were K(-), K(+), P1, P2, and P3 grups were 1.8923 ng/g, 1.8926 ng/g, 1.8926 ng/g, 1.8925 ng/g, and 1.8926 ng/g, respectively. Data was analysed using One Way ANOVA, and resulted p = 0.238. The conclusion was cinnamon ethanolic extract could not reduce kidney MDA levels in hyperglycemic wistar rat induced with alloxan. Keywords: Cinnamomum burmannii, polyphenols, alloxan, MDA
Efektivitas Ekstrak Etanol Daun Bayam Merah sebagai Hepatoprotektor terhadap Kadar ALP Serum Mencit yang Diinduksi Isoniazid (Hepatoprotective Activity of Red Spinach (Amaranthus tricolor L.) Leaves Ethanolic Extract on ALP Serum Mice Induced by Isoniazi Shinta Madyaning Wuri; Aris Prasetyo; Elly Nurus Sakinah
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Isoniazid (INH) is metabolized become hidrazine that depleted glutation (GSH) in hepatocytes, resulted imbalance oxidant and antioxidant along with increased of free radicals, which can increase alkaline phosphatase (ALP). Ethanolic extract of red spinach (Amaranthus tricolor L.) leaves contains antioxidant which can donates the electron so free radical can be stabilized. The aim of this study was to investigate the effect of ethanolic extract of red spinach leaves on ALP levels of mice induced by INH and its effective dose. This research used 28 mices that divided into seven groups: K(N) was given normal saline, K(-) was given INH 100 mg/kgBW, K1, K2, K3, K4 and K5 group which given INH 100 mg/kgBW peroral and after 2 hours would be given ethanolic extract of red spinach leaves in the dose 1,05 mg/20gBW, 2,1 mg/20gBW, 4,2 mg/20gBW, 8,4 mg/20gBW and 16,8 mg/20gBW for ten days. ALP serum was measured on the day 11th.The result of average ALP levels were K(N) 41,36 U/L, K(-) 110,97 U/L, K1 79,26 U/L, K2 71,34 U/L, K3 66,51 U/L, K4 62,82 U/L and K5 47,21 U/L. In conclusion the ethanolic extract of red spinach leaves could prevent the increasing of ALP levels and the effective dose of the extract was 6,75 mg/20gBW. Keywords: red spinach leaves, ALP, INH