Elly Nurus Sakinah, Elly Nurus
Fakultas Kedokteran Universitas Jember

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

Analog Rice Reduces Weight and Total Cholesterol Levels in Overweight and Hypercholesterolemic Rats Dewantari, Septiarina Putri; Hairrudin, Hairrudin; Sakinah, Elly Nurus
Jurnal Kedokteran Brawijaya Vol 31, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2020.031.01.2

Abstract

Overweight, whose prevalence is increasing, is caused by unbalanced consumption patterns such as high consumption of fat and can trigger hypercholesterolemia. Analog rice (AR), which has better nutritional content than ordinary rice, is thought to have beneficial effects on health. This study aimed to determine the potential of analog rice in reducing body weight and total cholesterol levels. A total of 24 male Wistar rats were divided into four groups, namely: I) control, II) ordinary rice treatment, III) analog rice 1 treatment (PBA1) and IV) analog rice 2 treatment (PBA2). The control group was given standard feed for 40 days ad libitum. Groups II, III, and IV were given a combination induction of high-fat diet (HFD) for 40 days ad libitum and intraperitoneal injection of streptozotocin (STZ) 35 mg/kg single dose on day 33. All groups were then given the treatment of diet for three weeks according to their respective groups, namely the standard, ordinary rice, analog rice 1, and analog rice 2 feed. Body weight and total cholesterol levels were measured in the pre-induction, post-induction/pre-treatment (pre-test), and post-treatment (post-test). The results of the study showed that the induction of the HFD-STZ combination carried out resulted in a significant increase in body weight and total cholesterol levels. Measurements made after dietary therapy showed that body weight and total cholesterol levels in the I and II groups did not change significantly, whereas those in the III and IV groups decreased significantly (p<0.05). This study concluded that the administration of analog rice could reduce body weight and total cholesterol levels in overweight and hypercholesterolemia rats. 
The Relation between Malondialdehyde (MDA) and Histopatological Appearance in male Wistar Rats Model Fridayanti, Febrina Sylva; Sulistyaningsih, Erma; Sakinah, Elly Nurus
Journal of Agromedicine and Medical Sciences Vol 1, No 1 (2015)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fractures are a serious health problem in Indonesia due to increasing prevalence. The healing process of fracture is disturbed by the oxidative stress that caused by imbalance quantity of free radical and antioxidant. An antioxidant such as polyphenol, which can be found in cocoa, is needed to suppress oxidative stress. The study aimed to investigate the effect of the ethanolic extract of cacao on fracture healing process in a rat model through MDA concentration and histopatological appearance. This study is in vivo experimental study with post-test only controlled group design. 30 male Wistar rats were randomized and divided into 5 groups. 1 group was rats without fractured. The negative control and three treatment groups were rats with fractured manually on left tibia under anesthesia and immobilized by bandage. The treatment groups treated with cocoa ethanolic extract in a dose of 125 mg/kgBW, 250 mg/kgBW, and 500 mg/kgBW orally for 21 days. The result showed that there was a significant different between the treatment groups and the negative control group on MDA concentration and histopatological appearance (p&gt;0,05). The corelation between them were strong and had negative direction (R=-0,771). The study concluded that cocoa ethanolic extract had a positive effect to supress oxidation stress and increases the number of osteoblast on fracture healing process.   Key words: cocoa ethanolic extract, polyphenol, fracture healing process, oxidative stress
The Effect Of Cholecalciferol On Fasting Blood Glucose In Streptozotocin-induced Hyperglycemia Mice Sakinah, Elly Nurus
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Glucose uptake into skeletal muscle cells require insulin-dependent and insulin independent signaling pathways, both leading to the translocation of glucose transporter-4 (GLUT4) to the plasma membrane. Insulin resistance occurs due to failure of insulin signaling to translocate GLUT4 resulting in the failure of glucose uptake and causing hyperglycemia. Cholecalciferol is known to have a function in regulating calcium homeostasis was shown to increase the synthesis of insulin and increasing insulin sensitivity. The purpose of this study is to explain the role of cholecalciferol to decreased fasting blood glucose in streptozotocin-induced hyperglyemia mice. 30 mice adapted for one week and then induced using 150mg/kgBW streptozotocin (STZ) intraperitoneally, After experiencing hyperglycemia mice were divided into 5 groups (n=6 each), Group I (hyperglycemic control), group II (25ng cholecalciferol), group III (50ng cholecalciferol), group IV (100ng cholecalciferol), and group V (metformin 300mg/kgBBB). Cholecalciferol given orally for 14th days. On day 15th the examination of fasting blood sugar levels were taken and the mice. Fasting blood sugar levels measured using a glucometer. Based on statistical analysis showed that there were significant differences in fasting blood sugar levels between treatment groups (p&lt;0,001). Based on univariate regression analyses there was negative correlation of cholecalciferol with fasting blood glucose (p&lt;0,001). Cholecalciferol may lower fasting blood sugar levels in hyperglycemia mice models. Keywords: cholecalciferol, fasting blood glucose, STZ.
Efek kandungan serat beras analog terhadap ekspresi GLUT4 otot rangka tikus diabetes Darajat, Azka; Sakinah, Elly Nurus; Hairrudin, Hairrudin
Jurnal Gizi Klinik Indonesia Vol 16, No 1 (2019): Juli
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1070.355 KB) | DOI: 10.22146/ijcn.31806

Abstract

Effect of analog rice’s fiber on skeletal muscles GLUT4 expression in diabetic rats Background: Disruption of glucose transportation in skeletal muscle through GLUT4 becomes a problem in diabetes. Analog rice that had been modified by adding dietary fiber could improve the expression of GLUT4.Objective: This study aims to know the effect of dietary fiber toward GLUT4 expression and to know the dietary fiber percentage in analog rice.Method: The research type is true experimental with post-test only group design. The samples consist of 24 male Wistar rats that are group into 4 groups (n=6 each group). Three groups were induced by giving a high-fat diet for 40 days and streptozotocin (STZ) 35 mg/kg BW was given at 33th day and one group was not induced. After the blood glucose level exceeded 135 mg/dl, the treatment was given. After 3 weeks, the rats were terminated and quadriceps femoris muscle tissue was taken for immunohistochemistry examination using rat GLUT4 polyclonal antibody. GLUT4 expression was quantified using an immunoreactive score (IRS-GLUT4). The data were analyzed using the Kruskal-Wallis test and Spearman test.Results: Statistical analyses showed that there were significant differences between groups with a moderate positive correlation (correlation coefficient=0,651; p=0,003).Conclusion: Dietary fiber in analog rice could improve skeletal muscle GLUT4 expression in Wistar rat diabetic model.