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All Journal Jurnal Teknologi Laboratorium Medical Laboratory Technology Journal Meditory : The Journal of Medical Laboratory Jurnal Skala Kesehatan JCPS (Journal of Current Pharmaceutical Sciences) Jurnal Riset Kefarmasian Indonesia Indian Journal of Forensic Medicine & Toxicology JPEMAS : Jurnal Pengabdian Kepada Masyarakat

leka lutpiatina, leka

Poltekkes Kemenkes Banjarmasin

Author-ID : 616250

Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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Improved Mycology Media Formula of Corn and Cow’s Hair For Accelerate Superficial Mycosis Growth Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Potato Dextrose Agar is growth and identification media of fungi that cause superficial mycoses, including Trichophyton rubrum and Trichophyton mentagrophytes with 10–14 days growth period. Modification media is needed to accelerate the growth of the fungus. Zea mays saccharata has a high carbohydrate, and cow’s hair contains high keratin, which can be used as a nutrient to replace fungal growth media. The study aimed to obtain a modified media formula containing Zea mays saccharata as a substitute for dextrose and cow's hair as a source of keratin to accelerate the growth of Trichophyton rubrum and Trichophyton mentagrophytes fungi. The modified media formula is mixing of Zea mays saccharata 15% to 25%, and cow's hair 20mg to 35mg. Acceleration of growth time based of when found microconidia and macroconidia, diameter measurements, and observations of texture and colonies color of Trichophyton rubrum and Trichophyton mentagrophytes. The study results are Zea mays saccharata media with the addition of cow hair for Trichophyton rubrum grew 11 days with 10%+20mg formula and grew only seven days with 25%+35mg formula. Modified media for Trichophyton mentagrophytes grew seven days for 10%+20mg formula, while it was only three days with 25%+35mg formula. The media formula with the fastest growth rate for Trichophyton rubrum and Trichophyton mentagrophytes was at a concentration of 25%+35mg. The Advanced Modified Media can become a patent medium for faster growth of superficial fungi at a lower cost. Further research needs to be done on how long this media can be stored for long-term use.

Sputum Quality of The Anytime And Outset for Examination Acid-Resistance Bacilli Leka Lutpiatina; Wahidah Wahidah; Nurhilaliah Nurhilaliah; Dinna Rakhmina; Rifqoh Rifqoh
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Diagnosis Tuberculosis (TB) can establish by microscopic examination of acid-resistant bacilli in the patient's sputum. Sputum quality greatly affects the diagnosis of TB, but the sputum collected by patients at community health centers sometimes does not meet the sputum quality requirements. The objective of this study was to assess the quality of sputum anytime and outset sputum on acid-resistant bacilli examination, from volume, color and viscosity parameters. The type of research used is descriptive observational. Respondents were suspected TB patients at the Marabahan and Aluh-aluh Public Health Centers of South Kalimantan province, Indonesia. Sputum samples taken were sputum anytime the first and outset sputum. The variables in this study are volume, color, and viscosity of sputum. The coloring of acid-resistant bacilli method Ziehl Nielsen. The results of the acid-resistant bacilli examination on sputum (86 specimens) found positive one (13%), positive three (2%), negative (85%). In morning sputum (86 specimens) found positive one (14%), positive three (2%), negative (84%). Sputum quality when with the good category as much as 36% and the quality of morning sputum with the good category as much as 55%. Conclusion Sputum outset has better quality than sputum at the anytime.

Produk Urikase dari Bacillus sp. Kontaminan Laboratorium leka lutpiatina
Medical Laboratory Technology Journal Vol. 1 No. 2 (2015): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Urease is an enzyme catalyst in the reaction of oxidation of uric acid into Allantoin. Oxidation of uric acid by the enzyme has become a basic principle of measurement of uric acid levels in the human body. This research to know urikase derived from the bacterium Bacillus sp that contaminate the air Laboratory of Microbiology Department of Health Analyst Banjarmasin. The research is descriptive survey. Airborne bacteria were isolated and identified for Bacillus sp. The identification is done by examination of macroscopic, microscopic and biochemical tests subsequently performed tests using nutrient agar medium containing 0.2% of uric acid. Results of the study showed there were two isolates of Bacillus sp and obtained clear zone on both these isolates. Conclusions of research there are contaminants Bacillus sp. as much as 40% in the laboratory. 100% of the yield urikase Bacillus sp. Urikase advice from Bacillus sp can be used as an alternative reagent uric acid probes spectophotometric method.

EFEKTIVITAS EKTRAK PROPOLIS LEBAH KELULUT (Trigona spp) DALAM MENGHAMBAT PERTUMBUHAN Salmonella typhi, Staphylococcus aureus DAN Candida albicans Leka Lutpiatina
Jurnal Skala Kesehatan Vol 6 No 1 (2015): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Propolis mempunyai khasiat sebagai antikanker, antivirus, antifungi dan antibiotika (Haryanto et al., 2012). Penelitian Agustina (2007), dihasilkan ekstrak propolis lebah asal Malang dapat mempengaruhi dan menghambat pertumbuhan bakteri gram positif Staphylococcus epidermidis pada konsentrasi 60% dan bakteri gram negatif Pseudomonas aeruginosa pada konsentrasi 70%. Penelitian mengenai efektivitas antibakteri dan anti jamur propolis dan lebah Trigona spp asal provinsi Kalimantan Selatan sejauh pengetahuan penulis belum pernah dilakukan. Tujuan umum penelitian ini untuk mengetahui konsentrasi yang efektif dari ekstrak propolis lebah kelulut (Trigona spp) dalam menghambat pertumbuhan bakteri Salmonella typhi Staphylococcus aureus dan Candida albicans. Hipotesis penelitian adalah Ekstrak propolis lebah kelulut (Trigona spp) efektif dalam menghambat pertumbuhan bakteri Salmonella typhi, Staphylococcus aureus dan Candida albicans. Jenis penelitian yang digunakan dalam penelitian ini adalah metode true eksperiment berupa rancangan-rancangan eksperimen sungguhan dengan menggunakan rancangan Postest Only Control Group Design (Notoatmodjo, 2010). Bahan penelitian adalah propolis dari sarang lebah kelulut (Trigona spp) yang ada di daerah Banjarbaru, dengan kriteria sarang yang bertekstur rapuh dan berwarna gelap. Data yang diperoleh ditabulasikan dan dilakukan analisis secara statistik dengan one way anova dan Kruskal Wallis α 0,05 pada tingkat kepercayaan 95%. Hasil penelitian konsentrasi yang efektif dari ekstrak propolis untuk Salmonella typhi dan Staphylococcus aureus adalah 100% dan tidak terdapat zone hambat untuk Candida albicans. Saran perlu dilakukan uji aktivitas antimikroba propolis lebah kelulut (Trigona spp) asal daerah Kalimantan Selatan terhadap candida albicans dengan metode dilusi. Kata Kunci : Ekstrak Propolis, Trigona spp, Salmonella typhi, Staphylococcus aureus, Candida albicans

Madu lebah kelulut (Trigona Spp.) dalam aktifitas terhadap bakteri Staphylococcus aureus resisten Muhammad Ma'ruf; Gina Alia Mawaddah; Nisa Nur Agistni Eriana; Farah Indah Swari; Syaidatul Aslamiyah; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Infection caused by Staphylococcus aureus bacteria becomes a very serious problem because of the increased resistance of these bacteria to various types of antibiotics. Honey has antibacterial activity because it contains water, acidity and inhibitor compounds namely flavonoids. Honey can be produced Trigona spp. This study aims to Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) and effectiveness of honey bee kelulut (Trigona spp.) Against Staphylococcus aureus resistant cephalosporin bacteria in vitro. This research is true experiment with postest only control group design. The results of the study were measured by MIC showed no clarity at concentrations of 60 mg / ml, 70 mg / ml, 80 mg / ml, 90 mg / ml and and clarity at concentrations of 100 mg / ml, and MBC at concentrations of 60 mg / ml of 151 colonies, 70 mg / ml of 105 colonies, 80 mg / ml of 55 colonies, 90 mg / ml of 16 colonies and 100 mg / ml of 0 colonies. The effective concentration in killing Staphylococcus aureus resistant cephalosporin is 100 mg / ml

Angka kuman udara ruang rawat inap anak dengan dan tanpa air conditioner (AC) di rumah sakit Raimunah Raimunah; Leka Lutpiatina; Jasmadi Joko Kartiko; Wahdah Norsiah
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Air microorganisms can be found in outdoor or indoor air, ventilation is a very important place in the exchange of indoor air. Ventilation system is divided into two, namely natural ventilation and artificial ventilation in the form of Air Conditioner (AC). This study aims to determine the description of the number of germs in the inpatient wards of children using Air Conditioner (AC) and non (AC) at the Hospital in Martapura city area. This type of research is descriptive survey, the population is the inpatient room of children using Air Conditioner (AC) and non (AC). Sampling using purposive sampling technique, that is as much as 8 room. The examination method used is by TPC (Total Plate Count) method. The results showed that the number of airborne bacterial infections using the Air Conditioner (AC) was 406 CFU/m3. The total number of non-living inpatients (AC) is 443 CFU/m3. The standard maximum number of microorganisms (CFU / m3) of treatment room according to Kepmenkes No.1204/ Menkes/SK/X/2004 is 200-500 CFU / m3.

Efektivitas Air Perasan Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Escherichia coli Ratih Dewi Dwiyanti; hana nailah; Ahmad Muhlisin; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 2 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Lime (Citrus aurantifolia) can be used for cough medicine, sputum laxative, influenza, and acne medication. Lime contains useful chemical compounds, one of which is essential oils and flavonoids which function as antibacterials and play a very important role in inhibiting bacterial growth. The purpose of this study was to determine the effectiveness of lime juice (Citrus aurantifolia) at a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% on the growth of Escherichia coli in vitro. This research is experimental with post test only control group design. The research sample was lime juice. Antibacterial power testing using well diffusion method. Antibacterial power parameters were determined by measuring the inhibitory zone (mm) formed around the growth of test bacteria on Muller Hinton Agar media. The results of the study showed that lime juice inhibition zones against the growth of Eschericia coli at concentrations of 40%, 50%, 60%, 70%, 80%, 90% and 100% were 7.25mm, 13.25mm, 14.25mm, 16mm, 17mm, 18.25mm, and 20.75mm. Based on linear regression test obtained significant value of 0.000 <α 0.05 so it can be concluded that there is an effect of lime juice on Eschericia coli growth in vitro. It is recommended that further research be carried out to test the inhibitory power of lime juice using other methods or against bacteria of other species.

Angka Kuman Es Batu Produksi Rumah Tangga Annisa Nur Yulianti; Ratih Dewi Dwiyanti; Wahdah Norsiah; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Air adalah kebutuhan dsar manusia, baik untuk keperluan sehari-hari seperti mandi, cuci, masak, dan minum. Air tidak hanya untuk air minun saja, tetapi dapat dibuat es batu yang memenuhi syarat bakteriologis. Penelitian bertujuan untuk mengetahui hasil TPC (Total Plate Count) pada es batu produksi rumah tangga di Kelurahan Sungai Besar. Penelitian ini bersifat survei deskriptif, sampel diperiksa dengan menggunakan metode TPC (Total Plate Count) dengan pengenceran 100 sampai 10-4. Sampel pemeriksaan berupa es batu yang diambil dari rumah produksi di Kelurahan Sungai Besar yang berjumlah 6. Hasil penelitian dari 24 sampel es batu produksi rumah tangga di Kelurahan Sungai Besar adalah 2 sampel yang memenuhi syarat dan 22 sampel tidak memenuhi syarat. Kesimpulan dari penelitian ini adalah 80% sampel tidak memenuhi syarat Standar Nasional Indonesia (SNI) 01-3553-2006. Saran penelitian lebih lanjut dengan melakukan identifikasi terhadap jenis bakteri yang terdapat dalam es batu tersebut.

Bactericidal Potential of Extract Citrus Hystrix D.C.Leaf Powder on Staphylococcus aureus and Salmonella typhi Elfira Maulidah; Anny Thuraidah; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 12 No 1 (2021): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jsk.v11i2.279

Infectious diseases can be caused by bacteria such as Staphylococcus aureus and Salmonella typhi. Treatment of infectious diseases can use antibiotics. However, antibiotics can cause side effects on users. Kuit Lime leaf (Citrus hystrix D.C.) contains secondary metabolites such as alkaloids, steroids, triterpenoids, tannins, and flavonoids used as antibacterial. This study aimed to determine the bactericidal potential of the kuit lime leaf extract powder on Staphylococcus aureus and Salmonella typhi. The type of research used is the experiment, and the research design used is the Posttest Only Control Group Design. The antibacterial activity test used was the liquid dilution of MIC and MBC. The results showed the MIC of Staphylococcus aureus and Salmonella typhi was 180 mg/mL and 120 mg/mL, while the MBC results against Staphylococcus aureus and Salmonella typhi are 190 mg/mL and 130 mg/mL. Data analysis in this research conducted descriptively. Based on descriptive analysis, it concluded that the bactericidal potential of the powdered extract of kuit lime against Staphylococcus aureus is at a concentration of 190 mg/mL and Salmonella typhi at a concentration of 130 mg/mL.

Potensi Air Rebusan Mengkudu (Morinda citrifolia L.) Dalam Menghambat Pertumbuhan Salmonella sp leka lutpiatina; Widiyawati Widiyawati
JCPS (Journal of Current Pharmaceutical Sciences) Vol 1 No 2 (2018): March 2018
Publisher : LPPM - Universitas Muhammadiyah Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (653.239 KB)

Salmonella sp merupakan bakteri patogen yang dapat menyebabkan Salmonellosis yang dapat ditangani dengan obat tradisional salah satunya mengkudu (Morinda citrifolia L.). Mengkudu memiliki kandungan senyawa kimia seperti antrakuinon, alizarin, L-asperuloside dan acubin yang berfungsi sebagai antibakteri. Tujuan penelitian ini adalah mengetahui adanya daya hambat dan daya bunuh serta pengaruh konsentrasi air rebusan buah mengkudu terhadap pertumbuhan Salmonella sp. Penelitian ini bersifat true eksperiment dengan rancangan prettest dan posttest control group design. Hasil penelitian Konsentrasi Hambat Minimal (KHM) terdapat pada konsentrasi 50 mg/ml sedangkan Konsentrasi Bunuh Minimal (KBM) belum terdapat pada semua konsentrasi. Jumlah koloni pada konsentrasi 30 mg/ml sebanyak 395 koloni, 35 mg/ml sebanyak 281 koloni, 40 mg/ml sebanyak 187 koloni, 45 mg/ml sebanyak 96 koloni dan 50 mg/ml sebanyak 6 koloni. Berdasarkan hasil penelitian disimpulkan ada pengaruh konsentrasi air rebusan buah mengkudu terhadap Salmonella sp dengan nilai signifikasi 0,000 dan besar pengaruh 99%. Perlu dilakukan penelitian menggunakan metode dilusi tabung terhadap air rebusan mengkudu (Morinda citrifolia L.) dengan konsentrasi yang lebih tinggi.

Co-Authors Abdul Khair Ahmad Muhlisin Annisa Nur Yulianti Annisa Rahmi Anny Thuraidah Dinna Rakhmina Elfira Maulidah Erpan Roebiakto Fadia, Fadia Farah Indah Swari Gina Alia Mawaddah hana nailah Helmiah, Tini Elyn jasmadi Joko Kartiko Muhammad Ma'ruf Nastiti Megawati Nisa Nur Agistni Eriana Noraida Noraida Nur Rizqi Amaliah Nurhilaliah Nurhilaliah Nurlailah Nurlailah, Nurlailah Raimunah Raimunah Ratih Dewi Dwiyanti Rifqoh Rifqoh Syaidatul Aslamiyah wahdah norsiah Wahidah Wahidah Widiyawati Widiyawati Yayuk Kustiningsih

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