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Penyimpanan dan Penambahan Kurma Ajwa Terhadap Aktivitas Antioksidan Infused Water Stroberi dan Wortel Luh Putu Rahayu Candra Dewi; Indah Lestari; Christ Kartika Rahayuningsih; Maruni Wiwin Diarti
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.274

Radikal bebas merupakan senyawa yang sangat reaktif dan dapat merusak molekul pembentuk sel dalam tubuh sehingga terjadi kemunduran fungsi organ dan memicu timbulnya penyakit degeneratif. Infused water merupakan alternatif dalam proses detoksifikasi radikal bebas sekaligus pemenuhan kebutuhan cairan tubuh harian. Stroberi banyak dijadikan sebagai bahan utama infused water karena kandungan vitamin C yang tinggi dan dikombinasikan dengan wortel yang kaya akan beta karoten sedangkan kurma ajwa dijadikan sebagai penambah cita rasa. Kandungan antioksidan total dalam infused water mudah mengalami degradasi fisik maupun kimia selama proses penyimpanan. Penelitian ini bertujuan untuk mengetahui pengaruh penyimpanan dan penambahan kurma ajwa terhadap aktivitas antioksidan infused water stroberi dan wortel. Jenis penelitian yang digunakan adalah eksperimental dengan rancangan post test only control group design yang dilaksanakan di Laboratorium Proteomik Institute Tropical Disease Universitas Airlangga dan Laboratorium Kimia AMAMI Jurusan Analis Kesehatan Poltekkes Kemenkes Surabaya pada bulan Oktober 2019 sampai Juni 2020. Penelitian ini terdiri dari dua kelompok infused water dengan dan tanpa penambahan kurma ajwa yang disimpan selama 0 jam sebagai kontrol, 2 jam dengan pengisian ulang pertama dan 4 jam dengan pengisian ulang kedua pada suhu ruang (±28oC) dan suhu refrigerator (±10oC). Masing - masing sampel diuji aktivitas antioksidan menggunakan metode DPPH yang dinyatakan dengan IC50 sedangkan untuk uji organoleptik dilakukan secara hedonik oleh panelis. Hasil analisis ANOVA menunjukkan bahwa penyimpanan dan penambahan kurma ajwa berpengaruh nyata terhadap aktivitas antioksidan infused water stroberi dan wortel (p<0,05). Aktivitas antioksidan tertinggi diperoleh dari infused water dengan penambahan kurma pada penyimpanan suhu ruang selama 2 jam dengan nilai IC50 sebesar 206,53 ppm yang menandakan aktivitas antioksidan sedang.

The Effectiveness Of Moringa Leaf Extract (Moringa oleifera) on Cadmium and LDL Cholesterol Levels in Blood as Indicators of Atherosclerosis in Cadmium (Cd) Induced White Rats (Rattus norvegicus) Vernanda Arsya Nabilla; Indah Lestari; Christ Kartika Rahayuningsih; Juliana Christyaningsih
Jurnal Teknokes Vol 15 No 3 (2022): September
Publisher : Jurusan Teknik Elektromedik, POLTEKKES KEMENKES Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/teknokes.v15i3.268

Moringa leaves (Moringa oleifera) have chelating agent properties that act as natural antioxidants in binding heavy metals and free radical scavengers, with cadmium being one of the heavy metals free radicals having toxic effects on the body. Exposure to cadmium can reduce the activity of lipoprotein lipase enzymes (LPL) which function in the triglycerides and free fatty acids catabolism process, and can increase cholesterol and triglyceride levels in the blood. This study aims to determine the effectiveness of Moringa (Moringa oleifera) on Cadmium chloride-induced white rats (Rattus norvegicus) using an experimental research design with a quantitative analysis technique. This study uses the level of cadmium and LDL cholesterol for the dependent variable and the dose of Moringa leaf extract (Moringa oleifera) for its independent variable. The samples used are white rats (Rattus norvegicus) aged 8-12 weeks with a weight of 150-200 grams, along with blood drawn from the heart for the specimen. The researcher conducted the study at the Faculty of Veterinary Medicine, Airlangga University; Surabaya Health Laboratory Center; and Surabaya Bakti Analysis Laboratory from October 2021 to June 2022 using Atomic Absorption Spectrophotometry (AAS) and Chemical Analyzer BS-200. The results show that the average value of cadmium and LDL cholesterol levels in white rats in treatment group 1 are 0.605 g/dL and 8.25 mg/dL, group 2 are 0.075 g/dL & 18.5 mg/dL; and group 3 are 0.08125 g/dL and 12 mg/dL. Based on the statistical analysis of the One-Way ANOVA test, the sig. p-value is > 0.05. Thus, the average value of cadmium and LDL cholesterol has no significant difference. Compared to the 400 mg/kgBW and 500 mg/kgBW, the 600 mg/kgBW dose has the best effectiveness in reducing LDL cholesterol in white rats (Rattus norvegicus) after being induced with cadmium chloride. This study recommends consuming fresh Moringa leaves (Moringa oleifera) as much as 66.522 grams per human/day as an additional antioxidant and chelating agent.

AKSI KOMPETENSI BERSAMA DI PANTI ASUHAN MISI NUSANTARA SURAKARTA Juliana Christyaningsih; Teresia Retna Puspitadewi; Lembunai Tat Alberta; Nuning Marina Pengge; Hotmaida Siagian; Diah Titik Mutiarawati; Christ Kartika Rahayuningsih; Dedi Kurniawan; Indah Lestari; Hery Sumasto; Lully Hani Endarini; Kiaonarni OW; Ira Puspita Sari; Evi Yunita N; Suliati; Darjati; Nur Hatijah; Khambali; Hadi Suryono; Demes Nurmayanti; Tatarini Ika Pipitcahyani; Astuti Setiyani; Siti Alfiah; Dina Isfentiani; Bambang Hadi Sugito; Retno Sasongkowati; Yusianti Silviani; N. S. Widodo
Jurnal Ilmiah Tatengkorang Vol 7 No 1 (2023): Jurnal Ilmia Tatengkorang
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Politeknik Negeri Nusa Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54484/tkrg.v7i1.553

Permasalahan sosial yang banyak terjadi di masyarakat adalah kaum marjinal yang terpinggirkan seperti anak terlantar, anak jalanan, pengemis, dan sebagainya. Salah satu upaya mengatasi masalah kaum marjinal yaitu dengan didirikan lembaga sosial berupa panti asuhan anak. Sejak masa pandemi, anak di Panti Asuhan Misi Nusantara Surakarta jarang mendapat layanan kesehatan dan ada keterbatasan ruang gerak anak-anak. Tujuan pengabdian masyarakat ini untuk membantu panti asuhan dalam memantau pertumbuhan dan perkembangan penghuni dan pengelola Panti Asuhan Misi Nusantara di Surakarta. Metoda pelaksanaan berupa pemeriksaan tumbuh kembang anak, Penyuluhan Hidup Bersih dan Sehat, pemeriksaan Status Gizi Anak, penyuluhan cara menyikat gigi yang benar, pemeriksaan kesehatan pengelola panti asuhan serta pelatihan penanggulangan bencana. Hasil pemeriksaan Kesehatan pada penghuni dan pengelola panti didapatkan sebagian besar (52,6 persen) penghuni panti berjenis kelamin laki-laki, hampir setengahnya berusia rata-rata 21-60 tahun, Sebagian besar (68,4 persen) tekanan darah kurang dari 140/90 mmHg, Sebagian besar (73,1 persen) berstatus gizi normal, hampir seluruhnya (94,4 persen) glukosa darah kurang dari 126 m/dL dan asam urat kurang dari 8 mg/dL. Secara umum keadaan warga panti asuhan dalam keadaan baik Social problems that often occur in society are abandoned children, street children, beggars, disabled people, neglected elderly, poor families, families with social problems, and so on. Children and poor people should have their human rights fulfilled by the State of Indonesia. However, in reality there are many children whose needs are not met, such as parents who are unable to provide for the child's needs or the child does not have parents. One effort to overcome this is to establish social institutions in the form of orphanages. Since the pandemic, children at the Mission Nusantara Surakarta Orphanage rarely receive health services and there is limited space for children to move. Theless than purpose of this community service is to assist the orphanage in monitoring the growth and development of residents and managers of the Mission Nusantara Orphanage in Surakarta. The implementation method is in the form of examinations on child growth and development, Counseling on Clean and Healthy Living, checking on the Nutritional Status of Children, counseling on how to brush their teeth properly, health checks for orphanage managers and training on disaster management. 6 percent) of the residents of the orphanage are male, almost half of them are aged 21-60 years, most (68.4 percent) have blood pressure less than 140/90 mmHg, most (73.1 percent) have normal nutritional status, almost all (94.4 percent) blood glucose less than 126 m/dL and uric acid less than 8 mg/dl. Similar activities are needed to see general body health and must be monitored periodically, so that the residents of the orphanage can find out their health status, especially now that they are still in a pandemic condition Covid-19

ANALISIS KADAR TIMBAL DALAM URIN PETUGAS OPERATOR DAN NONOPERATOR DI KECAMATAN KADEMANGAN KOTA PROBOLINGGO Pratiwi Dewi Ika; Lestari Indah; Endarini Lully Hanni
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.474 KB)

Gas stations are a source of lead exposure that comes from vehicle gas emissions and petrol fuel vapors. Gas station officers, both operators and nonoperators, have a risk level of lead exposure that can enter the body and then accumulate and excreted through urine by 75-80%. Lead exposure can endanger the health of gas station officers. The purpose of this study was to analyze differences in lead levels in the urine of gas station operators and nonoperators in Kademangan District, Probolinggo City. This research is an observational study with a cross sectional method which was conducted at the Surabaya Health Laboratory Center in October 2020 - June 2021. The sample used was 15 operators and 15 nonoperators who were taken by purposive sampling. The results showed the average value of lead levels in the urine for operators 0.0007213 ppm, indoor nonoperators 0.0006386 ppm and outdoor nonoperators 0.0008263 ppm. The conclusion of this study was that there was no significant difference in lead levels in the urine of gas station operators and nonoperators.

ANALISIS PAPARAN KADMIUM (Cd) DALAM DARAH TERHADAP KADAR SGOT DAN SGPT PADA PEROKOK AKTIF DAN PEROKOK PASIF DI WARUNG KOPI WILAYAH SURABAYA TIMUR Fitra Nur Febri; Lestari Indah; Rahayuningsih Christ Kartika
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.638 KB)

Cadmium is one of the chemicals contained in cigarettes, when consumed by humans in the long term and with sufficient intensity often can damage human organs such as the liver. This study aims to determine the exposure of cadmium to the levels of SGOT and SGPT in active smokers and passive smokers in a coffee shop in East Surabaya. This study used a correlational type of research, with a cross-sectional research design. There were 30 respondents who were divided into 2 groups, namely 15 active smokers and 15 passive smokers in a coffee shop in East Surabaya. The examination of cadmium used an Atomic Absorption Spectrophotomter (AAS), while the examination of SGOT and SGPT used the IFCC method (kinetic) with a photometer. The data were analyzed by Kolmogorov-Sminorv test and r-Pearson test. The results showed that there was no effect of high cadmium exposure in active smokers and passive smokers on SGOT and SGPT levels. The average cadmium in active smokers and passive smokers were 2.93 g/L and 1.30 g/L. while the levels of SGOT and SGPT in active smokers were 27.26 U/L and 36.86 U/L. The average levels of SGOT and SGPT in passive smokers were 19.73 U/L and 17.86, respectively. In these results it can be seen that the increase in cadmium levels does not affect liver function cells.

ANALISIS KADAR TIMBAL DALAM URIN PETUGAS OPERATOR DAN NONOPERATOR DI KECAMATAN KADEMANGAN KOTA PROBOLINGGO Pratiwi Dewi Ika; Lestari Indah; Endarini Lully Hanni
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Gas stations are a source of lead exposure that comes from vehicle gas emissions and petrol fuel vapors. Gas station officers, both operators and nonoperators, have a risk level of lead exposure that can enter the body and then accumulate and excreted through urine by 75-80%. Lead exposure can endanger the health of gas station officers. The purpose of this study was to analyze differences in lead levels in the urine of gas station operators and nonoperators in Kademangan District, Probolinggo City. This research is an observational study with a cross sectional method which was conducted at the Surabaya Health Laboratory Center in October 2020 - June 2021. The sample used was 15 operators and 15 nonoperators who were taken by purposive sampling. The results showed the average value of lead levels in the urine for operators 0.0007213 ppm, indoor nonoperators 0.0006386 ppm and outdoor nonoperators 0.0008263 ppm. The conclusion of this study was that there was no significant difference in lead levels in the urine of gas station operators and nonoperators.

ANALISIS PAPARAN KADMIUM (Cd) DALAM DARAH TERHADAP KADAR SGOT DAN SGPT PADA PEROKOK AKTIF DAN PEROKOK PASIF DI WARUNG KOPI WILAYAH SURABAYA TIMUR Fitra Nur Febri; Lestari Indah; Rahayuningsih Christ Kartika
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cadmium is one of the chemicals contained in cigarettes, when consumed by humans in the long term and with sufficient intensity often can damage human organs such as the liver. This study aims to determine the exposure of cadmium to the levels of SGOT and SGPT in active smokers and passive smokers in a coffee shop in East Surabaya. This study used a correlational type of research, with a cross-sectional research design. There were 30 respondents who were divided into 2 groups, namely 15 active smokers and 15 passive smokers in a coffee shop in East Surabaya. The examination of cadmium used an Atomic Absorption Spectrophotomter (AAS), while the examination of SGOT and SGPT used the IFCC method (kinetic) with a photometer. The data were analyzed by Kolmogorov-Sminorv test and r-Pearson test. The results showed that there was no effect of high cadmium exposure in active smokers and passive smokers on SGOT and SGPT levels. The average cadmium in active smokers and passive smokers were 2.93 g/L and 1.30 g/L. while the levels of SGOT and SGPT in active smokers were 27.26 U/L and 36.86 U/L. The average levels of SGOT and SGPT in passive smokers were 19.73 U/L and 17.86, respectively. In these results it can be seen that the increase in cadmium levels does not affect liver function cells.

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