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Journal : Journal of Fisheries

Komunitas Fitoplankton Pada Sistem Budidaya Intensif Udang Vaname, Litopenaeus vannamei di Probolinggo, Jawa Timur [Phytoplankton Community at Intensive Cultivation System of White Shrimp, Litopenaeus vannamei in Pr Nasrullah Bai Arifin; Muhammad Fakhri; Ating Yuniarti; Anik Martinah Hariati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 10 No. 1 (2018): Jurnal ilmiah perikanan dan kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v10i1.8542

Abstract

                                                    AbstrakFitoplankton merupakan sumber pakan alami pada budidaya udang di tambak. Produktivitas fitoplankton dapat meningkat seiring dengan bertambahnya kandungan nutrien di tambak. Sisa metabolisme dan pakan merupakan sumber nutrien bagi pertumbuhan fitoplankton di tambak. Penelitian ini bertujuan untuk mengevaluasi produktivitas dan mengidentifikasi jenis fitoplankton pada sistem budidaya intensif udang vaname, Litopenaeus vannamei. Penelitian ini dilakukan pada tiga petak tambak budidaya intensif udang vaname di Probolinggo, Jawa Timur. Pengambilan sampel air tambak dilakukan pada hari ke-17 dan hari ke-87 masa budidaya masing-masing dua kali setiap petak. Empat parameter lingkungan yaitu total ammonia nitrogen (TAN), nitrat, orthophosphate, total padatan tersupensi (TSS), dan klorofil diukur pada setiap sampel air tambak. Selain itu, kami juga mengidentifikasi dan menghitung fitoplankton pada setiap sampel. Hasil penelitian ini menunjukkan bahwa produktivitas pada ke tiga tambak berkisar antara 22.893,83 kg/ha sampai 23.600,61 kg/ha dengan ukuran panen 12,74 g/ekor sampai 14,35 g/ekor. Selama masa budidaya, konsentrasi TAN, TSS, dan klorofil meningkat seiring dengan semakin lamanya masa budidaya. Sementara itu, rerata kandungan nitrat dan orthophosphate cenderung menurun seiring bertambahnya masa budidaya. Jenis fitoplankton yang teridentifikasi yaitu dalam genus Oocystis, Chlorella, Nannochloropsis, Chaetoceros, Stephanodiscus, Nitzschia, Coscinodiscus, Cyclotella, dan Ulothrix. Fitoplankton dari kelompok Chlorophyta merupakan jenis yang dominan pada tambak 1 dan 2, sedangkan tambak 3 didominasi oleh kelompok Diatom. Penelitian ini mengindikasikan bahwa keberadaan fitoplankton di tambak mendukung ketersediaan pakan alami dan lingkungan yang baik bagi budidaya udang.                                                      AbstractPhytoplankton is a source of natural feed for shrimp cultivation in the pond. Phytoplankton productivity increases by the increased nutrient content in the pond. Feed and metabolic waste is the sources of nutrient for phytoplankton growth. This study aimed to evaluate productivity and identify phytoplankton at intensive white leg shrimp, Litopenaeus vannamei cultivation system. This study was conducted at three intensive white shrimp located in Probolinggo, East Java. Samples were collected on the early and the late of culture period (day 17 and 87 after stocking). Four environmental parameters including total ammonia nitrogen (TAN), nitrate, orthophosphate, total suspended solids (TSS), and chlorophyll-a were measured. Identification and density of phytoplankton were also performed of each pond. The result showed that the productivity of three ponds was 22,893.83 kg/ha to 23,600.61 kg/ha with an average size of 12.74 g to 14.35 g. During culture period, the concentration of TAN, TSS, and chlorophyll-a tended to increase. Meanwhile, the average of both nitrate and orthophosphate tended to decline. Several phytoplankton identified in this study were in the genus of Oocystis, Chlorella, Nannochloropsis, Chaetoceros, Stephanodiscus, Nitzschia, Coscinodiscus, Cyclotella, and Ulothrix. Phytoplankton of the group Chlorophyta is predominance for pond 1 and 2, while pond 3 was dominated by phytoplankton in the group of Diatom/Baccillariophyta. This study indicated that the presence of phytoplankton in the pond provides natural feed and good environmental condition for shrimp cultivation.
Spore production and sporulation efficacy of Bacillus subtilis under different source of manganese supplementation [Produksi Spora dan Efisiensi Sporulasi Bacillus subtilis dengan Suplementasi Mangan dari Sumber yang Berbeda] Ating Yuniarti; Nasrullah Bai Arifin; Muhammad Fakhri; Anik M. Hariati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 11 No. 2 (2019): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v11i2.15250

Abstract

AbstractBacillus is a species widely used as a probiotic in the aquaculture industry. The Bacillus spores have more advantages than their vegetative ones, and an addition of minerals such as calcium, magnesium, and manganese can improve the spore production. The purpose of this study was to determine the effect of different sources of manganese on the production and sporulation efficacy of B. subtilis SB3. The sources of manganese used in this study were manganese chloride (MnCl2) and manganese sulfate (MnSO4) at the concentration of 10 mM. Media without manganese supplementation was used as a control. The results showed that there was a significant effect of different manganese sources on the spore production of B. subtilis SB3. The highest spore production was found in media with MnCl2 supplementation with the total spore of 8.77 x 107 spores. mL-1. However, spore production with MnSO4 supplementation was still higher (22.7%) compared to that without manganese supplementation. The decrease in spore production with MnSO4 supplementation was possible due to the sulfate inhibition. The high spore production in media with MnCl2 supplementation was also preceded by the high production of vegetative cells from B. subtilis SB3 (2.54 x 108 cells. mL-1). The results indicated that manganese could stimulate both vegetative cell growth and its spores. The highest sporulation efficacy (35%) was also achieved in media with MnCl2 supplementation. On the other hand, the germination rate of B. subtilis SB3 spores was not influenced by manganese supplementation.Abstrak Bacillus adalah species yang banyak digunakan sebagai probiotik pada industri akuakultur. Dalam bentuk spora, species ini lebih banyak mempunyai kelebihan dibandingkan dalam bentuk vegetatifnya dan peningkatan produksi sporanya dapat dilakukan dengan penambahan mineral seperti kalsium, magnesium dan mangan. Tujuan penelitian ini adalah untuk mengetahui pengaruh sumber mangan yang berbeda terhadap produksi dan efisiensi sporulasi B. subtilis SB3 indigenous akuatik. Sumber mangan yang dipakai dalam penelitian ini adalah mangan klorida (MnCl2) dan mangan sulfat (MnSO4) sebanyak 10 mM dan sebagai kontrol digunakan media tanpa suplementasi mangan. Hasil penelitian menunjukkan bahwa terdapat pengaruh yang nyata penggunaan sumber mangan yang berbeda terhadap produksi spora. Produksi spora tertinggi didapatkan pada media dengan suplementasi MnCl2 sebanyak 8,77 x 107 spora. mL-1. Sedangkan produksi spora dengan suplementasi MnSO4 juga masih lebih tinggi (22,7%) dibandingkan tanpa suplementasi magan. Penurunan produksi spora pada media dengan penambahan mangan sulfat diduga karena adanya penghambatan oleh sulfat. Tingginya produksi spora pada media dengan suplementasi MnCl2 sebelumnya juga didahului dengan tingginya produksi sel vegetatif dari B. subtilis SB3 (2,54 x 108sel. mL-1). Hal ini menunjukkan bahwa mangan dapat menstimulasi baik pertumbuhan sel vegetatif dan sporanya. Efisiensi sporulasi tertinggi juga dicapai pada media dengan suplementasi MnCl2 sebesar 35%. Di sisi lain, kemampuan germinasi spora B. subtilis SB3 tercatat sama dan tidak dipengaruhi oleh suplementasi mangan.
Effects of Various Nitrogen Sources on the Growth and Biochemical Composition of Chlorella sp. Ating Yuniarti; Muhammad Fakhri; Nasrullah Bai Arifin; Anik Martinah Hariati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.43182

Abstract

Highlight Research Each species of microalga has a preferable nitrogen source for their optimal growth. The nitrogen sources in the grown media affected the growth rate and biochemical composition of Chlorella FNUB01. (NH2)2CO (urea) was found to be the best alternative nitrogen source for Chlorella FNUB01. For producing 1 g of Chlorella FNUB01, the use of urea reduced the cost of medium by 72.6%. Abstract Chlorella sp. is a potential microalgae species to be produced commercially for feed, growth accelerator, and immuno-modulator in fish and shrimp culture. This study aimed to evaluate the various nitrogen sources on the growth, biomass production, and biochemical composition of Chlorella sp. FNUB01. The nitrogen sources used in this study were urea (NH2)2CO, potassium nitrate (KNO3), and ammonium nitrate (NH4NO3). Sodium nitrate (NaNO3) was used as a control as it is a part of the commercial medium BG-11. Generally, the sources of nitrogen in the media affected the growth and chemical composition of Chlorella sp. FNUB01. This green microalga grew better in the urea-containing medium which accounted for 1.5 times the concentration of that cultured in BG-11 (40 x106 cells. mL-1). Meanwhile, this microalgae species experienced the lowest growth when cultured in NH4NO3-containing medium. The biomass productivity of Chlorella sp. FNUB01 cultured in urea (0.93 g.L-1) was comparable to those grown with NaNO3 as the N source. A similar pattern was recorded for protein, chlorophyll, and carotenoid content as these biochemical contents were affected by N availability in the medium. Urea was an alternative low-cost N source for the culture of Chlorella sp. FNUB01. Replacement of NaNO3 with urea could reduce the cost of the medium by 72.6%.