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All Journal Jurnal Akuakultur Indonesia The Journal of Experimental Life Sciences (JELS) El-Hayah : Jurnal Biologi Jurnal Harpodon Borneo Journal of Innovation and Applied Technology Research Journal of Life Science agriTECH Jurnal Sumberdaya Akuatik Indopasifik AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Media Akuakultur Samakia: Jurnal Ilmu Perikanan Journal of Aquaculture and Fish Health JURNAL BAHARI PAPADAK Journal of Fisheries & Marine
Ating Yuniarti
Department Of Aquaculture, Faculty Of Fisheries And Marine Science, Universitas Brawijaya. Malang. Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Social Sciences Other

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Journal : The Journal of Experimental Life Sciences (JELS)

 1 
Immunostimulant Activity of Neem Leaf (A. indica A. Juss) Ethanol Fraction on Tilapia (Oreochromis niloticus) Rika Putri; Sri Andayani; Ating Yuniarti
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1110.631 KB) | DOI: 10.21776/ub.jels.2018.008.03.08

Abstract

Intensive cultivation of Tilapia with high stock density and continuous artificial feeding will decrease the water quality and increase the growth of pathogenic bacteria. That situation led to the fish stressed so that the fish become the target of bacterial pathogen attacks. Alternatively, the environmental friendly treatment using herbal immunostimulant to solve that problem is required. The herbal in this study used neem leaf (Azadirachta indica A. Juss). The aim of this study was to evaluate ethanol fraction of neem leaf on immunostimulant activity of Oreochromis niloticus. In this study, the extraction method used maceration. Fractionation methods were using Thin Layer Chromatography (TLC) and Column Chromatography (CC). The biological component was analyzed using Phytochemical, FTIR and GC-MS methods. TLC test showed that ethyl acetate: n-hexane (1:1, v/v) result in brightest luminescence colors on the plates of silica gel under UV light (365 nm). The chromatography column from the ethanol extract of neem leaf (A. indica A. Juss) showed that separation of the fraction was done based on the formation of color, which obtained 5 (five) types of fractions. The results of the phytochemical test for the fraction 4 of neem leaf contains two compounds, i.e. flavonoids (+) and triterpenoids (++). The FTIR test showed that fraction 4 of neem leaves contain a class of terpenoid and flavonoid compounds. GCMS test results on fraction 4 of the neem leaves (A. indica A. Juss) obtained the high peak, which has 4 terpenoid compounds, 1 compound of flavonoid, and 1 type of lauric acid. The total leukocyte of the fish test increased after treated with fraction 4 of neem leaf (A. indica A. Juss) compared with control on day 7 after injection, i.e. 25.29 x 104 cells.mL-1.Keywords: fraction, leukocyte, Neem Leaves, Tilapia, Phytochemicals.
Phytochemical Screening and Antimicrobial Activity of Roselle (Hibiscus sabdariffa L.) Flower Extract Against Aeromonas hydrophila Sitti Khairul Bariyyah; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.809 KB) | DOI: 10.21776/ub.jels.2019.009.02.01

Abstract

Medicinal plants as an antimicrobial agent may provide an alternative way to replace the use of antibiotics to control disease agents in aquaculture. Roselle flowers (Hibiscus sabdariffa L.) has been used in many sectors as a source of functional food, natural coloring agents as well as antimicrobial agents. The objectives of this study were to evaluate the phytochemical compound in methanolic extract of roselle flower and their antimicrobial activity against Aeromonas hydrophila. The phytochemical composition of roselle flower was evaluated using phytochemical screening and FTIR. While the antimicrobial activity was performed by using the disc diffusion agar and co-culture with A. hydrophila. The results of phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, and tannins. The results of FTIR revealed that Roselle flower extract had the main phenolic compounds. The result of disc diffusion and co-culture method indicated that the roselle flower extract had antibacterial activity against A. hydrophila. This antibacterial activity depended on the concentration applied.Keywords: antibiotics, Co-culture, Disk diffusion, flavonoid, Hibiscus sp.
Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila Annisa Farhana Dewi; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1120.058 KB) | DOI: 10.21776/ub.jels.2019.009.02.02

Abstract

Aeromonas hydrophila is a type of gram-negative bacteria that can harm humans and major animals that have poikilotherm properties such as fish and shrimp. These bacteria can cause haemorrhages in fish until death and causing fish farming production to failure. The use of medicinal plants has been trusted by people all over the world to overcome various disease problems, one of which is a disease caused by bacteria. Plantago major L. is known that able to inhibit the growth of both gram-positive and gram-negative bacteria. The purposes of this study were to identify the extracted compounds contained in Plantago major L., using FTIR and UV-VIS spectrophotometer and to evaluate antibacterial activity against A. hydrophila. The results of this study showed that crude extracts of Plantago major L. contained polar compounds such as phenols, flavonoids, saponins, and tannins. The presence of phenols and flavonoids was confirmed by the results of FTIR and UV-VIS. Furthermore, the crude extract of Plantago major L. significantly inhibited the growth of A. hydrophila (P <0.05).Keywords: Antibacterial, Co-culture, Extraction, FTIR, UV-VIS.
Effect of Prebiotic and Probiotic Fish Feed on Physical, Chemical and Biological Quality of Feed M. Indra Wahyu Pratama; Anik Martinah; Ating Yuniarti
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.04

Abstract

The limitations of fish in digesting food depend on the presence of enzymes protease, amylase, and lipase that react with substrates in the digestive channel of fish. Supplementation methods can be performed to increase fish growth by adding prebiotics and probiotics to the feed. This study aims to determine the influence of the difference in the length of time fermented prebiotic feed (sweet potato extract) and probiotics (Bacillus megaterium) on the quality of feed. The results of the physical analysis of fish feed showed the color looks brown, fishy smell, texture, and general conditions of feed seemed completely normal. Chemical analysis showed that the proteins were best improved in B3 treatment, with the period of fermentation approximately 72 hours. Biological analysis suggests that the longer the fermentation time taken, the higher the abundance of bacteria obtained.Keywords: B. megaterium, chemistry and biology of fish feed, physical analysis, sweet potatoes (Ipomoea batatas L)
Co-Authors A.P.W Marhendra Abd Rahem Faqih Agung Setia Abadi Andriawan, Soni Anik Hartinah Mariati Anik M. Hariati Anik Martina Hariati Anik Martinah Annisa Farhana Dewi Arief Prajitno Arief Prajitno Arief Prajitno, Arief Arifin, Nasrullah Bai Arning Wilujeng Ekawati Bao, Hung Tran Budianto Budianto C Anam Cheng, Phillip T. Citra Satrya Utama Dewi Dewa Gede Raka Wiadnya Diana Permatasari Endang Yuli Herawati Happy Nursyam Hariati, Anik Martinah M. Indra Wahyu Pratama Marsoedi Marsoedi Mohamad Fadjar Muammar Kadhafi Muhammad Fakhri Muhammad Fakhri Muhammad Fakhri Muhammad Fakhri Muhammad Fakhri Nasrullah Bai Arifin Nasrullah Bai Arifin Nasrullah Bai Arifin Nasrullah Bai Arifin Rani Yuwanita Rika Putri Rusmawanto, Rusmawanto Septi Anitasari Sitti Khairul Bariyyah Sri Andayani Sri Andayani Sri Andayani Sri Andayani Suleman Suleman Suleman Suleman Wahyu Endra Kusuma