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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 30 Documents
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Search results for , issue "Vol 8, No 2 (2016): September 2016" : 30 Documents clear
The Effects of Dose Rhizoctonia Binucleat (BNR) and Phosphorus to Nitrate Reductase Activity (NRA) and Chlorophyll of Vanilla Seedling (Vanilla planifolia Andrews) Haryuni, Haryuni; Dewi, Tyas Soemarah Kurnia
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6328

Abstract

Vanilla (Vanilla planifolia Andrews) is one of the important exported commodities in Indonesia. Indonesia is one of top five major vanilla exporters in the world, that produce the high quality of Indonesian vanilla with high vanillin content (2.75%). The aims of this research were to determine the effects of dose binukleat Rhizoctonia (BNR) and phosphorus as well as the interaction of the nitrate reductase activity (NRA) and chlorophyll of the vanilla seedling (Vanilla planifolia Andrew). Method in this research used completely randomized factorial design, by involving two factors (dose of BNR inoculation and Phosphor). The first factor is without inoculation and inoculation BNR (M0, M1, M2, M3) wich consists of (0,5, 10, 15) g/polybag, the second factor is the dose of phosphorus fertilizer (P0, P1, P2, P3) which consists of (0, 3, 6, 9) g/polibag. The results showed that the inoculation dose of BNR and doses of phosphorus not significant and lower levels of NRA and chlorophyll while the interaction dose of BNR and phosphorus significantly and increase levels of NRA and chlorophyll of vanilla seedling. Nitrate Reductase Activity and chlorophyll has important role in metabolism process as a plant growth indicator.How to CiteHaryuni, H., & Dewi, T. S. K. (2016). The Effects of Dose Rhizoctonia Binucleat (BNR) and Phosphorus to Nitrate Reductase Activity (NRA) and Chlorophyll of Vanilla Seedling (Vanilla planifolia Andrews). Biosaintifika: Journal of Biology & Biology Education, 8(2), 141-147.
Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol Habibah, Noor Aini; Moeljopawiro, Sukarti; Dewi, Kumala; Indrianto, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6632

Abstract

Stelechocarpus burahol is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro S. burahol from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration of plant growth regulators i.e. picloram (5, 7.5 and 10 mg/L) and 2, 4-D (10, 15 and 20 mg/L) under dark condition. Induction of callus formation started on the 20.29th to the 29.86th days. Medium supplemented with Picloram and dark state proved to be the best condition for optimum callus induction from mesocarp explants of S. burahol. Callus grown on medium with the addition of 7.5 mg/l Picloram produces the highest flavonoid. The maximum production of the secondary metabolite was obtained from 8 weeks old callus. However, by the time of callus ageing, its output has declined. It could be concluded that callus cultures from mesocarp S. burahol can be used for flavonoid production.How to CiteHabibah, N. A., Moeljopawiro, S. Dewi, K. & Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp ofStelechocarpus burahol. Biosaintifika: Journal of Biology & Biology Education, 8(2), 214-221.
Harmful Algal in Banyuasin Coastal Waters, South Sumatera Aryawati, Riris; Bengen, Dietriech Geoffrey; Prartono, Tri; Zulkifli, Hilda
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6356

Abstract

Phytoplankton have important as food-chain major component and primary production of marine environment. However, high abundance of phytoplankton could give harmful effects toward water ecosystem. Moreover, they could produce toxic substances that will be accumulated within their consumer. This accumulation could be dangerous for human or animals.This research were aimed to determine and calculatespecies of harmful algae in Banyuasin coastal waters. The study was conducted on April, June, August, October and December of 2013, and in February 2014, at ten stations. Phytoplankton samples were taken vertically using plankton nets. In the form of cone-shaped with a diameter of 30 cm, length 100 cm and mesh size 30 ?m.The result showed that there are 35 genera of phytoplankton. That have been found and consisted of four groups; Bacillariophyceae, Dinophyceae, Cyanophyceae and Chlorophyceae. 13 species were identified as Harmful Algal (Chaetoceros, Coscinodiscus, Nitzschia, Skeletonema, Thalassiosira, Alexandrium, Ceratium, Dinophysis, Noctiluca, Protoperidinium, Prorocentrum, Anabaena dan Oscillatoria), with seven of them were known for having toxin (Nitzschia, Alexandrium, Dinophysis, Protoperidinium Prorocentrum, Anabaena and Oscillatoria). Monitoring result showed that the highest number of species of potential harmful algal blooms (HABs) occured in June and the highest abundance occured in August, especially Chaetoceros and Skeletonema.How to CiteAryawati, R., Bengen, D. G., Prartono, T., & Zulkifli, H. (2016). Harmful Algal in Banyuasin Coastal Waters, South Sumatera. Biosaintifika: Journal of Biology & Biology Education, 8(2), 231-239.
The Effectiveness of Local Plants from Lom and Sawang Ethnics as Antimalarial Medicine Helmi, Henny; Afriyansyah, Budi; Ekasari, Wiwied
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5437

Abstract

Native people or ethnic societies that live in endemic malaria islands such as in Bangka Island and Belitung Island have used many medicinal plants to cure malaria. Leaves of kesembung (Scaevola taccada (Gaertn Roxb), roots of kebentak (Wikstroemia androsaemofolia Decne), and roots of medang mencena (Dapniphyllum laurinum (Benth) are the examples. This research was aimed to investigate the present of some biochemical compound and evaluate the antimalarial activity of ethanol extract of the plants against Plasmodium falciparum 3D7 in vitro. The IC50 level was determined through visual observation under microscope over 5000 of giemsa-stained erythrocytes then analyzed by probit analysis. Results showed that kebentak root ethanol extract was effective to inhibit P. falciparum 3D7 with level 0.485 g/mL. Furthermore, the IC50 level of kesembung leaves and medang root were 44.352 g/mL and 1486.678 g/mL respectively. Phytochemical test result showed that kebentak leaf ethanol crude extract contained triterpenoid, kesembung root contained phenol and tannins; moreover, medang root contained alkaloid, saponin, and triterpenoid.How to CiteHelmi, H., Afriyansyah, B. & Ekasari, W. (2016). The Effectiveness of Local Plants from Lom and Sawang Ethnics as Antimalarial Medicine. Biosaintifika: Journal of Biology & Biology Education, 8(2), 193-200.
Impact of Distance from the Forest Edge on The Wild Bee Diversity on the Northern Slope of Mount Slamet Widhiono, Imam; Sudiana, Eming
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5058

Abstract

In agricultural landscape in northern slope of Mount Slamet, diversity of wild bee species as pollinator depend on forested habitats. This study aimed to assess the effects of distance from the forest edge on the diversity of wild bees on strawberry and tomato crops. This study was conducted from July 2014 to October 2014. The experimental fields contained tomato and strawberry with a total area of 4 ha (2 ha each) and divided into five plots based on distance from the forest edge (0, 50, 100, 150, and 200 m). Wild bee was catched with kite netting in 7.00 -9.00 in ten consecutive days. Wild bee diversity differed according to distance from the forest edge, the highest value was at 0 m for strawberry plots (H = 2.008, E = 0.72 and Chao1= 16) and for tomato plots, the highest diversity was at 50 m from the forest edge (H = 2.298, E = 0.95 and Chao1= 11) and the lowest was at 200 m in both plots. Wild bee species richness and abundance decreased with distance, resulting in the minimum diversity and abundance of wild bee at 200 m from forest edge in both crops.How to CiteWidhiono, I., & Sudiana, E. (2016). Impact of Distance from the Forest Edge on The Wild Bee Diversity on the Northern Slope of Mount Slamet. Biosaintifika: Journal of Biology & Biology Education, 8(2), 148-154.
The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured Nugroho, Rudy Agung; Manurung, Hetty; Saraswati, Dewi; Ladyescha, Deasy; Nur, Firman Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6519

Abstract

This research aimed to determine the phytochemicals content of Terminalia catappa leaves extract (TCL) and its effects on the survival and blood profiles of ornamental fish (Betta sp) Ninety fish were randomly assigned into six triplicates groups and reared in various concentration of TCL: 0 (control), 125, 250, 375, 500, 625 ppm for 30 days. Temperature, Dissolve oxygen (DO), and pH were monitored during the trial. After 30 days, survival, Red Blood Cells (RBC), White Blood Cells (WBC), haemoglobin (Hb), lymphocyte, and total protein serum (TPS) were analyzed. Based on the phytochemicals test, saponin, triterpenoid, quinon, phenolic, tannin, and flavonoid were detected on the TCL. Temperature and DO were not affected by any concentration of TCL. The lowest pH (5.05) was found in fish medium immersed with 625 ppm of TCL. Adding TCL above 375 ppm resulted in significantly higher survival, RBC, and Hb. The highest WBC was found in fish immersed with 625 ppm whereas the lowest lymphocyte was found in fish immersed with 375 of TCL. However, immersing any various concentration of TCL did not affect on the TPS. In summary, immersing TCL above 375 ppm is beneficial to enhance survival, RBC, WBC, and Hb of Betta sp.How to CiteNugroho, R. A., Manurung, H., Saraswati, D., Ladyescha, D. & Nur, F. M. (2016). The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured. Biosaintifika: Journal of Biology & Biology Education, 8(2), 240-247.
Ethnotaxonomical Study of Mole Crab (Crustacea:Hippoidea) on Coastal Community of Cilacap Bhagawati, Dian; Anggoro, Sutrisno; Zainuri, Mohammad; Syarani, Lachmudin
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6491

Abstract

Local wisdom is a cultural heritage that needs to be preserved. The Cilacaps coastal communities have traditional taxonomy knowledge that hasnt been informed, especially about mole crab. This study aimed to explore the ethnotaxonomy knowledge of sand crabs (Crustacea: Hippoidea) in Widarapayung coastal communities. The purposive snowball sampling was used for the method, followed by observation and semi-structured interviews with 30 respondents. Data were analyzed descriptively based on observations and interviews. The results showed that the coastal communities in Widarapayung tourist areas could be divided into five groups, namely catchers, collectors, processors, traders, and consumers. The catcher was able to recognize and describe three types of sand crabs based on the shape of the body and their catchment areas. Captured crabs had the local name of yutuk jambe (Emerita emeritus Linnaeus 1767), the appellation was based on carapace shape that resembled the shape of areca fruit; Yutuk bathok (Hippa adactyla Fabricius 1787), had the carapace shape resembling a coconut shell, and yutuk Kethek (Albunea symmysta Linnaeus 1758), had ornaments on the carapace surface similar with a monkey face, there were dense setae on the edge of the carapace, spiny long antennas and aggressively pinch when captured. The knowledge and skills of sand crabs classification and local name entitlement acquired by the people were in accordance with the Berlins model.How to CiteBhagawati, D., Anggoro, S., Zainuri, M. & Syarani, L. (2016). Ethnotaxonomical Study of Mole Crab (Crustacea:Hippoidea) on Coastal Community of Cilacap. Biosaintifika: Journal of Biology & Biology Education, 8(2), 222-230.
The Potential Fruit Crop of Cibodas Botanical Garden Normasiwi, Suluh; Surya, Muhammad Imam
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5235

Abstract

As an institute for ex situ plant conservation of high mountains, Cibodas Botanical Garden (CBG), has more than 1652 species and 8140 specimens of plant collections. An inventory of potential fruit crop in CBG which will support the conservation program had never been done before. The aim of this activity is to determine its potential collections as fruit crop. Descriptive analysis was used to analyze all the data achieved from registration unit and catalogue of (CBG). The results showed that 422 numbers of collections from 31 family, 56 genus and 114 species have high potential as a fruit crop. Moreover, Cibodas Botanical Garden has 74% collection of indigenous fruit (included 85 species) and 61% collection of underutilize fruit (included 68 species) from the total number of fruit plant collections. Most of potential plant collections are able to be developed as an edible fruit crop in Indonesia in order to enhance local food security through diversification of fruit crop.How to CiteNormasiwi, S., & Surya, M. I. (2016). The Potential Fruit Crop of Cibodas Botanical Garden. Biosaintifika: Journal of Biology & Biology Education, 8(2), 206-213.
Protein Profile and Hematological Parameters of Mice post Injected with Irradiated Plasmodium berghei Rosadi, Imam; Syaifudin, Mukh; Elfidasari, Dewi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6341

Abstract

Indonesia had a high number of malaria cases where the treatments are only perceived by 10% of patients in health facilities. The resistance of Plasmodium sp. to drugs causes the difficulty of controlling malaria. The use of irradiation to produce malaria vaccine material is being explored until nowadays. In previously study that dose rate of gamma irradiation delivered to parasite was at the low dose rate (380 Gy/hour) which produced several doses that not be able to attenuate the parasite. This research aimed to increase dose rate (740 Gy/hour) and to determine the dose irradiation effect of 150-250 Gy to the protein profile, erythrocyte and leukocyte numbers in mouse blood infected by P. berghei erythrocytic stage. Results showed that there was an influence of dose rate of 740 Gy/hour for doses of 150-250 Gy in treated mice and no difference in erythrocyte, leucocyte and body weight with negative control. Dose of 150 Gy was the optimal dose to attenuate parasites, whereas doses more than 150 Gy effectively killed the parasites. The changes of protein profile marked by the appearance of higher number of bands at molecular weight of 30-37 kDa that predicted as merozoite surface protein of P. berghei and/or antibodies elicited in mouse.How to CiteRosadi, I., Syaifudin, M., & Elfidasari, D. (2016). Protein Profile and Hematological Parameters of Mice post Injected with Irradiated Plasmodium berghei. Biosaintifika: Journal of Biology & Biology Education, 8(2), 155-164
A Preliminary Study of Bryophytes in Enggano Island, Bengkulu, Indonesia Nadhifah, Ainun; Surya, Muhammad Imam
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5239

Abstract

Enggano Island is one of the outer islands that belongs to the Province of Bengkulu. Furthermore, there is very limited information about the diversity of bryophyte from Sumatera, especially in lowland forest, Enggano Island. The aim of this research was to provide the initial information related to the diversity of bryophytes in Enggano. The research was conducted in six forests i.e. primary, secondary and degraded forests. The results showed that 32 number of collection from 14 families, 21 genera, and 32 species were identified. Leceuneaceae was common family for liverworts while the mosses family was dominated by Hypnaceae. None of the hornworts were found in this study. Some species identified (Taxyphyllum sp., Vesicularia sp., Riccardia sp., and Thuidium sp.) have the potential benefit and biological activity. Two genera were new records to Sumatra, Gongylanthus, and Symphyogyna. Moreover, Gongylanthus sp. and Symphyogyna sp. have important records related to the habitat.How to CiteNadhifah, A., & Surya, M. I. (2016). A Preliminary Study of Bryophytes in Enggano Island, Bengkulu, Indonesia. Biosaintifika: Journal of Biology & Biology Education, 8(2), 201-205.

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