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HAYATI Journal of Biosciences

hayati
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Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

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Articles 8 Documents

Search results for , issue "Vol. 13 No. 3 (2006): September 2006" : 8 Documents clear

The Immunohistochemical Profile of Superoxide Dismutase (SOD) in the Liver Tissue of Hypercholesterolemic Rats TUTIK WRESDIYATI; MADE ASTAWAN; LUSIA YUNI HASTANTI
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

This study was conducted to observe intracellular antioxidant cooper,zinc-superoxide dismutase (Cu,Zn-SOD) in liver tissue of rats under hypercholesterolemic condition by using immunohistochemical technique. A total of twenty male Wistar rats were used for this study. Those rats were divided into two groups; (i) control group and (ii) hypercholesterolemic group, which were fed died containing 1% cholesterol for eight weeks. Rat livers were taken at the end of treatment, and processed by using paraffin embedding standard method. The tissues were stained immunohistochemically to Cu,Zn-SOD. Observation of Cu,Zn-SOD content in the tissue was performed qualitatively in the cytoplasm and quantitatively in the nucleus of hepatocytes based on colour intensity of enzyme reaction product. The profile of antioxidant-Cu,Zn-SOD decreased (P < 0.05) in the hypercholesterolemic group compared to the control group. Key words: Superoxide dismutase (SOD), hypercholesterolemia, liver, rat

The Enhancement of Catharanthine Content in Catharanthus roseus Callus Culture Treated with Naphtalene Acetic Acid DINGSE PANDIANGAN; NELSON NAINGGOLAN
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

The research aim was to examine the enhancement of catharanthine content in Catharanthus roseus callus culture added with different concentration of Naphtalene Acetic Acid (NAA). NAA treatment produced callus that formed hairy roots. Fresh and dry weight of callus increased as the increasing of NAA concentration. The catharanthine content of C. roseus callus culture was increased by adding NAA as well. The highest catharanthine content was found in 2.5 ppm NAA added callus. Key words: Catharanthus roseus, callus, catharanthine, NAA

Synergism of Lumbricus rubellus and Pseudomonas putida Pf-20 in Inducing Resistance to Cucumber Mosaic Virus WIWIEK SRI WAHYUNI; HARDIAN SUSILO ADDY; BUDI ARMAN; TRI CANDRA SETYOWATI
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

Both Lumbricus rubellus and Pseudomonas putida decompose soil organic matters. The population of P. putida Pf-20 increased if L. rubellus was introduced to the cucumber growth medium. The process of organic decomposition was much better if the medium was introduced with both L. rubellus and P. putida Pf-20, compared to the medium contained only either one of those organisms. The activity of L. rubellus may serve to provide nutrients for both the cucumber and P. putida. The role of P. putida to reduce disease severity was increased if L. rubellus was introduced to the growth medium. The synergism of these two organisms, reduced either the level of disease severity to CMV-48 and C/N ratio of medium, but increased the content of available phosphor and potassium. Key words: Lumbricus rubellus, Pseudomonas putida Pf-20, disease severity of CMV

Changes of Gibberellin and Total Sugar Content in Flower Developmental Stages of Mangosteen I NYOMAN RAI; ROEDHY POERWANTO; LATIFAH KOSIM DARUSMAN; BAMBANG SAPTA PURWOKO
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

The objectives of this experiment were to study the changes of gibberellic acid and total sugar content in flower developmental stages of mangosteen. The result showed that flower development of mangosteen consisted of four stages: induction, differentiation, maturition of flower organs, and anthesis. Floral induction was microscopically characterized by the swelling of the basal structure of the new shoot. It was found that induction stage of mangosteen flowering was characterized by sharp decrease of gibberellic acid (GA3, GA5, GA7) and increase of total sugar content of leaf. On the other hand, it was found that leaf of the non-flowering shoot apices had high gibbrellic acid and low total sugar. Key words: mangosteen, flowering, induction, gibberellic acid

Inheritance of Blast Resistance (Pyricularia grisea Sacc.) on Interspecific Crossing between IR64 and Oryza rufipogon Griff DWINITA WIKAN UTAMI; HAJRIAL ASWIDINNOOR; SUGIONO MOELJOPAWIRO; IDA HANARIDA; REFLINUR REFLINUR
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

Blast disease affected by Pyricularia grisea causes high percentage of yield losses in rice production. The improvement of durable Blast resistance is difficult due to the complexity of the inheritance of this trait. This study was conducted to evaluate the genetic control and inheritance of Blast resistance trait in interspesific population between IR 64 (accepted Indonesian rice type, medium resistant to Indonesian Blast pathogen) and Oryza rufipogon (AA genome; acc. No.IRGC#105491; donor for Blast resistance). Six populations, i.e. P1, P2, F1, F2, BCP1, and BCP2, were inoculated with three Indonesian races of Blast pathogen. The disease intensity was determined on the basis of disease leaf area (DLA). The three types of gene interactions (additive, dominance, and epistatis) were significantly involved in this trait. Among the digenic epistasis interactions, all of the additive x additive [i], additive x dominance (j) and dominance x dominance (l) contributed to the trait. Broad-sense heritability ranged from 50.30 up to 91.20%, while the narrow heritability ranged from 16.98 up to 73.20%. The presence of additive gene effect indicated that selection of this trait is promising. Key words: inheritance, Blast resistance, wild rice, Oryza rufipogon

Characterization of Lipopolysaccharides of Bradyrhizobium japonicum KDR 15 Heavy Metal Tolerant ALFI DATIN ZAUQIAH; TEDJA -IMAS; DWI NINGSIH SUSILOWATI
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

The lipopolysaccharide (LPS) of Bradyrhizobium japonicum KDR 15 heavy metal tolerant strain was isolated by miniphenol-water extraction and yielded LPS in phenol and water phase. The LPS KDR 15 was further characterized by sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS PAGE) and showed many bands distributed from an area of high until low molecular weight (LPS IA, IB, and II). Composition analysis of the LPS had been done after acetic acid 1% hydrolysis. The polysaccharide portion consist of glucose, sucrose, galactose, mannose, xylose, arabinose, rhamnose, ribose, glucosamine, and 3-deoksi-D-manno-oktulosonat (KDO). Lipid A portion consisted of C16:0 and C18:1. The LPS also contained 0.02% of protein and 1.7% of phosphate. The presence of functional groups that shows negative charge densities such as phosphate and carboxyl within LPS KDR 15 assumed to be a potentially binding sites for accumulating heavy metals. Key words: Bradyrhizobium japonicum, heavy metal tolerant, lipopolysaccharides

Feeding Rate of Soil Animals in Different Ecosystems in Pati, Indonesia RAHAYU WIDYASTUTI
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

The feeding activity of soil animals was measured by using bait lamina test in three main ecosystems, i.e. the teak forest, home garden and rainfed paddy field. Two additional ecosystems in rainfed paddy field, i.e. the old (permanently established bund around paddy fields) and new bunds were examined as well. Three blocks of bait-lamina sticks (each block consisting of 16 individual sticks) were exposed at each location. The bait lamina were retrieved from the soil after two days and visually assessed. Each hole is designated as Ã¢â&sbquo;¬Å“fedÃ¢â&sbquo;¬Â (perforated) or Ã¢â&sbquo;¬Å“non-fedÃ¢â&sbquo;¬Â hole. The feeding rate is measured as the absolute number of Ã¢â&sbquo;¬Å“fedÃ¢â&sbquo;¬Â holes. Soil animals in the old bunds showed the highest feeding activity (55.20%), followed by home garden (39.10%), rainfed paddy field (16.50%), teak forest (15.60%), and new bund (7.80%). The frequency of animals attack to the bait strips also indicated the similar pattern as their feeding activity, i.e. high in the old bunds (0.90), followed by home garden (0.70), teak forest (0.40), new bunds (0.40) and rainfed paddy field (0.30), respectively. Key words: soil animals, feeding activity, bait-lamina test

Ecotoxicological Assessment of Aquatic Genotoxicity Using the Comet Assay KHUSNUL YAQIN
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

Comet assay is a novel biological analysis, which is a sensitive, flexible, simple, rapid, and inexpensive method to assess aquatic genotoxicant. Since Singh and co-workers developed the method in 1988, its use has increased exponentially in various fields. This review discourses on the application of this assay in aquatic ecosystems. Various types of cells from various aquatic organisms have been tested by various genotoxicant both direct- and indirect-acting using the comet assay. The applications of this assay suggest that it is a useful assay to assess aquatic genotoxicants. However, there are some factors, which should be taken into account when using this assay as aquatic ecotoxicological assessment device such as inter-animal and cell variability. Key words: comet assay, aquatic genotoxicant, aquatic organism, ecotoxicological assesment

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