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PENGARUH PEMBERIAN EKSTRAK ETANOL DAN KLOROFORM DAUN SIRSAK TERHADAP KOLESTEROL TOTAL DAN TRIGLISERIDA PADA TIKUS YANG DIINDUKSI ALOKSAN Firmansyah, Deni; Bachri, Moch Saiful; Nurkhasanah, Nurkhasanah
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Annona muricata plant is a medicinal plant using by research and drug for human healthy including diabetes mellitus. This research aimed to determine the effect of ethanol extract and chloroform extract of the sirsak leaf as antidiabetes mellitus. This research was conducted in 9 groups of male Wistar rats consisting of 5 rats per group, consisting of normal control, alloxan control, glibenclamide control dose of 10 mg/Kg BW, group of ethanol extract sirsak leaf dose of 50 mg/Kg BW; 100 mg/Kg BW; 200 mg/Kg BW, and group of chloroform extract sirsak leaf dose of 50 mg/kg BW; 100 mg/Kg BW; 250 mg/Kg BW. Tests carried out for 2 weeks. It also conducted assays of total flavonoids and histopathological tests of pancreatic Î² cells. Results of this research showed that the ethanol extract ofÂ sirsak leaves dose of 200 mg/Kg BW has activity in decreasing blood glucose levels better than any other group. Results of the ethanol extract and chloroform extract all doses except the ethanol extract treatment group dose of 200 mg/kg BW and 100 mg/kg BW after a 14-day look is still high triglyceride levels and decrease in total cholesterol (TC) were seen in all group Ethanol extract dose of 200 mg / kgBWÂ has a good potential to reduce levels of triglycerides and total cholesterol. The conclusion of this research is the ethanol extract of sirsak leaf have activity antidiabetic mellitusand decreased total cholesterol.Â

PENGARUH PEMBERIAN KOMBINASI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP BERAT BADAN DAN JUMLAN NODUL TIKUS SPRAGUE DAWLEY BETINA YANG DIINDUKSI 7,12 DIMETILBENZ(Î±) ANTRASEN (DMBA) Nurani, Laela Hayu; Mursyidi, Achmad; Widyarini, Sitarina
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Pasak Bumi roots contain quasinoid compounds that have carcinogenesis inhibitory, imunomudulator, antiulcer, and antimalaria effects. This study aims to determine the effect of a combination of ethyl acetate fraction of Pasak Bumi roots and doxorubicin on DMBA-induced white mice by weight and palpation observation. The mice were divided into 8 groups. Each group consisted of 7 female Sprague Dawley mice. Group 1 (normal) was only given food and drink, Group 2 was given fraction of ethyl acetate of Pasak Bumi roots (100 mg/kg body weight, Group 3 (positive control) was given doxorubicin (1.17 mg/kg body weight) 1 times a week for 5 weeks, Group 4 was given DMBA (20 mg/kg) two times a week for 5 weeks, Groups 5 was given DMBA (20 mg/kg) + doxorubicin (1.17 mg/kg), Group 6 was given DMBA (20 mg / kg) + ethyl acetate fractions of Pasak Bumi roots (100 mg / kg), Group 7 was given DMBA (20 mg / kg) + doxorubicin (1.17 mg / kg) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg) and Group 8 doxorubicin ( 1.17 mg / kg body weight) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg). Observations were made on the basis of body weight of mice and tumor incidence by palpation at week 6 of treatment. The result ofÂ this research shows that the administration of ethyl acetate fraction of Pasak Bumi roots and doxorubicin combination can increase body weight of the mice and decrease the number of tumors compared to single doxorubicin.

EFEKTIFITAS PEMBERIAN PROBIOTIK TERHADAP DURASI DIARE ANAK DI RUMAH SAKIT PKU MUHAMMADIYAH BANTUL YOGYAKARTA Mulyani, Sri; Perwitasari, Dyah Aryani; Umam, Nurcholid
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Diarrhea is the second leading cause of childrenâs death worldwide, including in Indonesia.With regard to reducing the number of death, it is highly needed to provide a quick and accurate treatment. In clinical practice, prebiotics has been widely used as a treatment, particularly in the case of acute diarrhea in children. However, it is still not recommended by WHO. This study is aimed to determine the treatment pattern of diarrhea in childrenâs ward at PKU Muhammadiyah Bantul Hospital and determine whether probiotic supplementation on standard therapy results a decrease in the duration of diarrhea which is better than only the standard therapy in the management of acute diarrhea in children. This study used retrospective cohort design using data from medical records with diagnose acute diarrhea or acute gastroenteritis (ICD A09). The data was collected from January to December 2014, at childrenâ2 ward, PKU Muhammadiyah Bantul Hospital, Yogyakarta. Data was divided into three groups: (i) Group I those who were treated by using standard therapeutic with rehydration and zinc, (ii). Group II those who were treated by combination of rehydration, zinc and probiotics, (iii) Group III those who were treated with rehydration and probiotics. Data duration of diarrhea as an outcome of the effectiveness of probiotics analyzed statistically to determine differences in outcomes between groups. Among the three groups, Group I, Group II and group III gained an average duration of diarrhea 66.33 (SD 21.66) hours, 55.32 (SD 19.07) hours and 54.79 (SD 17.69) hours, respectively. The statistical test shows that there is significant different from diarrhea duration among the three groups (p = 0.014). The test results between probiotic groups (II and III) and the control group is significantly different with p-value 0.018 and 0.011, respectively. While the group II and group III do not differ significantly (p = 0.898). Treatment pattern therapy of diarrhea in PKU Muhammadiyah Bantul Hospital divided into three kinds of therapy. The first is rehydration therapy and zinc supplementation, the second is rehydration therapy and zinc supplementation and probiotics, the third is rehydration therapy and probiotics. Probiotic supplementation is proven effective in reducing the duration of diarrhea significantly compared to patients who were received standard therapy.

AKTIVITAS ANTIFUNGI EKSTRAK DAUN BELUNTAS, JAWER KOTOK, DAN SIRIH SERTA KOMBINASINYA TERHADAP Candida albicans Yuniarni, Umi; Lukmayani, Yani
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Piper betle, Coleus scutellarioides and Pluchea indica have been used empirically to overcome vaginal discharge and scientifically proven to inhibit the growth of Candida albicans. The goal of this research was to search for a combined extract with effective antimicrobial activity against Candida albicans. Single extract,combination of two and three extracts evaluated antimicrobial activity against Candida albicans using agar diffusion method. Inhibitory effect of Piper betle showed the greatest activity against Candida albicans compared to other single extract. Combination of Piper betle and Pluchea indica (26.57Â±0.56 mm) gave greater inhibition than two otherÂ combination extracts (Piper betle and Coleus scutellarioides (25.17Â±0.96 mm); Coleus scutellarioides and Pluchea indica (22.73Â±0.95 mm).The combination of Piper betle, Pluchea indica and Coleus scutellarioides gave inhibition of 27.53Â±1.10 mm. extracts did not give a better diameter compared to the betle extract.

PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN KELOR (Moringa oleifera L.) TERHADAP AKTIVITAS ANALGESIK DAN ANTIINFLAMASI MELALUI EKSPRESI ENZIM SIKLOOKSIGENASE Rini Sulistyawati; Pramita Yulli Pratiwi
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Kelor (Moringa oleifera L.) contains flavonoid as a major of bioactive constituent. Quercetin as a flvonoid group inhibits COX-2 in inflammatory process. This study aimed to determine the effect of etanol extract of the kelor leaves on the analgesics and antiiinflamatory in mice. Etanol extract of the kelor leaves were evaluated for analgesics activity through writhing assay test at doses of 12.5; 25 and 50 mg/kgBW using Â Swiss albino mice. On the other hand antiinflammatory assay was performed by carrageenan induced paw edema of the etanol extract of the kelor leaves at 35; 70 and 140 mg/kgBW. Aspirin and sodiumdiclofenac were employeed as a standard for analgesic and antiinflammatory studies respectively. The result of the analysis show that etanol extract of the kelor leaves at doses 25 and 50 mg/kgBW decrease the number of writhing so that it have an analgesic power of 76.41Â±2.73% and 80.41Â±5.20%. Etanol extract of the kelor leaves at dosage 140 mg/kgBW show 24.30Â±2.960% antiinflammatory power and 46.37Â±6.434% on decreased COX-2 expression.

EFEKTIFITAS PEMBERIAN PROBIOTIK TERHADAP DURASI DIARE ANAK DI RUMAH SAKIT PKU MUHAMMADIYAH BANTUL YOGYAKARTA Sri Mulyani; Dyah Aryani Perwitasari; Nurcholid Umam
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Diarrhea is the second leading cause of childrenâ€™s death worldwide, including in Indonesia.With regard to reducing the number of death, it is highly needed to provide a quick and accurate treatment. In clinical practice, prebiotics has been widely used as a treatment, particularly in the case of acute diarrhea in children. However, it is still not recommended by WHO. This study is aimed to determine the treatment pattern of diarrhea in childrenâ€™s ward at PKU Muhammadiyah Bantul Hospital and determine whether probiotic supplementation on standard therapy results a decrease in the duration of diarrhea which is better than only the standard therapy in the management of acute diarrhea in children. This study used retrospective cohort design using data from medical records with diagnose acute diarrhea or acute gastroenteritis (ICD A09). The data was collected from January to December 2014, at childrenâ€™2 ward, PKU Muhammadiyah Bantul Hospital, Yogyakarta. Data was divided into three groups: (i) Group I those who were treated by using standard therapeutic with rehydration and zinc, (ii). Group II those who were treated by combination of rehydration, zinc and probiotics, (iii) Group III those who were treated with rehydration and probiotics. Data duration of diarrhea as an outcome of the effectiveness of probiotics analyzed statistically to determine differences in outcomes between groups. Among the three groups, Group I, Group II and group III gained an average duration of diarrhea 66.33 (SD 21.66) hours, 55.32 (SD 19.07) hours and 54.79 (SD 17.69) hours, respectively. The statistical test shows that there is significant different from diarrhea duration among the three groups (p = 0.014). The test results between probiotic groups (II and III) and the control group is significantly different with p-value 0.018 and 0.011, respectively. While the group II and group III do not differ significantly (p = 0.898). Treatment pattern therapy of diarrhea in PKU Muhammadiyah Bantul Hospital divided into three kinds of therapy. The first is rehydration therapy and zinc supplementation, the second is rehydration therapy and zinc supplementation and probiotics, the third is rehydration therapy and probiotics. Probiotic supplementation is proven effective in reducing the duration of diarrhea significantly compared to patients who were received standard therapy.

PENGARUH PEMBERIAN KOMBINASI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP BERAT BADAN DAN JUMLAN NODUL TIKUS SPRAGUE DAWLEY BETINA YANG DIINDUKSI 7,12 DIMETILBENZ(Î±) ANTRASEN (DMBA) Laela Hayu Nurani; Achmad Mursyidi; Sitarina Widyarini
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Pasak Bumi roots contain quasinoid compounds that have carcinogenesis inhibitory, imunomudulator, antiulcer, and antimalaria effects. This study aims to determine the effect of a combination of ethyl acetate fraction of Pasak Bumi roots and doxorubicin on DMBA-induced white mice by weight and palpation observation. The mice were divided into 8 groups. Each group consisted of 7 female Sprague Dawley mice. Group 1 (normal) was only given food and drink, Group 2 was given fraction of ethyl acetate of Pasak Bumi roots (100 mg/kg body weight, Group 3 (positive control) was given doxorubicin (1.17 mg/kg body weight) 1 times a week for 5 weeks, Group 4 was given DMBA (20 mg/kg) two times a week for 5 weeks, Groups 5 was given DMBA (20 mg/kg) + doxorubicin (1.17 mg/kg), Group 6 was given DMBA (20 mg / kg) + ethyl acetate fractions of Pasak Bumi roots (100 mg / kg), Group 7 was given DMBA (20 mg / kg) + doxorubicin (1.17 mg / kg) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg) and Group 8 doxorubicin ( 1.17 mg / kg body weight) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg). Observations were made on the basis of body weight of mice and tumor incidence by palpation at week 6 of treatment. The result ofÂ this research shows that the administration of ethyl acetate fraction of Pasak Bumi roots and doxorubicin combination can increase body weight of the mice and decrease the number of tumors compared to single doxorubicin.

UJI ANTIOKSIDAN EKSTRAK ETANOL DAUN DAN KULIT BATANG MANGGIS Garcinia mangostana L. Diniatik Diniatik; Suparman Suparman; Dewi Anggraeni; Ibnu Amar
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Antioxidant was a compound which possessed an activity as scavenger of free radicals. This study was aimed to examine the antioxidant activity of ethanolic extract from leaves and skin barks of Garcinia mangostana. Antioxidant activity were evaluated by using DPPH method for ethanolic extract in serial concentration of 800, 400, 200 and 100ug/mL. The result found that ethanolic extract of leaves and skin barks of Garcinia mangostana showed antioxidant activity with IC50 value of 674.947 ug/mL and 565.759 ug/ml, they were eleven and ten times weaker than d-Î±-tocopherol (IC50 57,114 ug/mL) which were used as a control. In conclusion, EE from leaves and skin barks of Garcinia mangostana exhibited an activity as scavenger of free radicals from DPPH.

AKTIVITAS ANTIMIKOSIS EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) Didi Rohadi
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Prevalence of Candidiasis is still in high level. Some antimycosis have been less effective so it needs to explore novel sources of antimycosis, one of candidate is the soursop leaves (Annona muricata L.) The aims of this study to observe antimycosis activity of the ethanol extract of soursop leaves at concentration of 15 %, 30 %, and 60 % against Candida albicans ATCC 10231 with agar diffusion method . The results showed that all of the ethanol extract of soursop leaves have exhibited antimycosis activity which concentration-dependent.

AKTIVITAS ANTIFUNGI EKSTRAK DAUN BELUNTAS, JAWER KOTOK, DAN SIRIH SERTA KOMBINASINYA TERHADAP Candida albicans Umi Yuniarni; Yani Lukmayani
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Piper betle, Coleus scutellarioides and Pluchea indica have been used empirically to overcome vaginal discharge and scientifically proven to inhibit the growth of Candida albicans. The goal of this research was to search for a combined extract with effective antimicrobial activity against Candida albicans. Single extract,combination of two and three extracts evaluated antimicrobial activity against Candida albicans using agar diffusion method. Inhibitory effect of Piper betle showed the greatest activity against Candida albicans compared to other single extract. Combination of Piper betle and Pluchea indica (26.57Â±0.56 mm) gave greater inhibition than two otherÂ combination extracts (Piper betle and Coleus scutellarioides (25.17Â±0.96 mm); Coleus scutellarioides and Pluchea indica (22.73Â±0.95 mm).The combination of Piper betle, Pluchea indica and Coleus scutellarioides gave inhibition of 27.53Â±1.10 mm. extracts did not give a better diameter compared to the betle extract.

Home Page

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Muhammad Syahrir

Contact Email
m.syahrir7406@unm.ac.id

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Journal Mail Official
nurkhasanah@pharm.uad.ac.id

Editorial Address
Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia Kode pos 55164

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

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Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Muhammad Syahrir

Contact Email m.syahrir7406@unm.ac.id

Phone -

Journal Mail Official nurkhasanah@pharm.uad.ac.id

Editorial Address Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia Kode pos 55164

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Muhammad Syahrir

Contact Email
m.syahrir7406@unm.ac.id

Phone
-

Journal Mail Official
nurkhasanah@pharm.uad.ac.id

Editorial Address
Jl. Prof. Dr. Soepomo, S.H., Janturan, Warungboto, Umbulharjo, Yogyakarta, Indonesia Kode pos 55164

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA