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All Journal YARSI Medical Journal Cermin Dunia Kedokteran Journal of General-Procedural Dermatology & Venereology Indonesia
Kariosentono, Harijono
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Health Professions Medicine & Pharmacology Public Health

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Journal : Journal of General-Procedural Dermatology

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The effect of injection of ADSC compared to APPE on collagen density in aging skin (animal study) Pricilla, Erlina; Julianto, Indah; Kariosentono, Harijono; Budiani, Dyah R; Fibrianto, Yuda Heru; Rindiastuti, Yuyun; Veraida, Ainun
Journal of General - Procedural Dermatology & Venereology Indonesia Vol. 2, No. 2
Publisher : UI Scholars Hub

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Introduction: Aging is a biological process that can induces changes to the structural integrity and physiological function of skin. Structural changes of skin are a result of dermal atrophy, decreased collagen, the loss of subcutaneous fat, the loss of inherent elasticity, and increased melanogen.1,5,6 From the definition itself, a stem cell characterized by its ability to self-renew and also have an ability to differentiate along multiple lineage pathways.2,4 ADSC which is a kind of mesenchymal stem cell from human adipose tissue, have some potential properties as stem cells derived from bone marrow.4,7 Objective: The aim of this study was to verify the comparison effect of ADSCs and their secretome for skin aging treatment. Methods: ADSC were isolated from lipoaspirates obtained from healthy donors after obtaining written consent and ethical approval using liberase enzymatic digestion, washed with steril PBS and centrifuged. After the phase, ADSC were seeded directly after isolation with liberases and cultured, then produced the conditioning media (AAPE) by hypoxia amplifies.3,4,6,7 We analysed the ADSCs (2,5 x 105 cells) and their secretomes (0,1ml in 0,4ml NaCl 0,9%) by subcutaneous injection on the back of a rat (with range age 48 weeks), and followed began every two weeks after injected until six weeks and stained with Van Giesson staining, for measured the density of collagen.3,5,6 Results: Collagen density was increased after ADSC injected to the skin. From statistical analysis showed there was a significant result of collagen density compared to control group and AAPE (p<0.05). We conclude that ADSC had anti-aging potential or regenerative by stimulating collagen synthesis of dermal fibroblast. ADSC will be as new modality treatment for aging skin in future.
The comparison of skin irritation level between topical cajeput oil and telon oil: A pilot study Ilona, Sri Esa; Cahyono, Aris; Ellistasari, Endra Yustin; Irawanto, Muh. Eko; Kariosentono, Harijono
Journal of General - Procedural Dermatology & Venereology Indonesia Vol. 2, No. 3
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Background: Cajuputi/Cajeput/Melalueca leucadendron oil is a type of essential oil, widely known and used by Indonesian for infants, children, and elderly. It is also included in telon oil compositions (±42%). Excessive application of cajeput oil and telon oil may lead to skin irritation. Methods: This randomized single-blind control trial was conducted with 10 subjects who met the inclusion criteria. Four areas with diameter of 10 mm each were made on the flexor surface of the upper arm. Area 1 was designated as negative control. Area 2 to 4 were pre-irritated with tape stripping for 40 ± 10 times using Nachitape®, followed by repeated open application test (ROAT) with cajeput oil, telon oil and alcohol (positive control). The skin irritation was examined in 15 minutes after each cycle by measuring transepidermal water loss (TEWL) using Tewameter® TM300 (Courage-Khazaka, Germany) and erythema using Mexameter® MDD4. Data were analyzed using One-way ANOVA with p<0.05 considered significance. Results: The average TEWL and erythema of cajeput oil (15.59; 345.42) were the highest compared to telon oil (12.63; 316.60), alcohol (13.87; 319.06) and negative control (7,48; 296.68). All treatment groups had significant differences in TEWL (p<0.000) and erythema (p<0.002) when compared to the negative control. However, cajeput oil showed the largest difference. Conclusion: Cajeput oil caused the most irritation compared to alcohol and telon oil. Therefore, it should be used with caution.
Co-Authors Ahmad Fiqri, Ahmad Anggraeni, Reti Aulia Rahman Budiani, Dyah R Cahyono, Aris Dewi, Susanti Rosmala Dhamayanti, Marsita Endy Diana, Rina Ellistasari, Endra Yustin Ilona, Sri Esa Imelda Betaubun, Ance Indah Julianto, Indah Irawanto, Muh. Eko Mustifah, Etty Farida Nurrachmat Mulianto Pricilla, Erlina Rafika Paramitasari, Anggana Rieka Sulaikha, Khalida Rinandari, Ummi Rini Hastuti Risman, Muhammad Tri Irfanti, Rakhma TUTIK RAHAYU Veraida, Ainun Wirawan, Eka Putra Yuda Heru Fibrianto Yuyun Rindiastuti