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Potensi Ekstrak Kelakai (Stenochlaena palustris (Burm.F) Bedd) terhadap Kadar Tumor Necrosis Factor-Alfa (TNF-α) pada Mencit BALB/c yang Diinfeksi Plasmodium berghei ANKA Margono, Denny P.N.H.; Suhartono, Eko; Arwati, Heny
Berkala Kedokteran Vol 12, No 1 (2016): Februari 2016
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v12i1.359

Abstract

Abstract: Malaria remains a major public health problem in most tropical and subtropical countries, including Indonesia. Severe malaria has a high mortality rate despite treatment with effective antimalarial drug. Pro-inflammatory cytokines such as Tumor Necrosis Factor-alfa (TNF-α) is raised in severe malaria.  In South Kalimantan, the kelakai (Stenochlaena palustris (Burm.f) Bedd) has few uses for treat fever and infectious diseases.  It contains bioactive substances, such as flavonoids, steroids, and alkaloids which have been reported to exert multiple biological effects, including anti-inflammatory action.   The aim of this study is to find out the potential of kelakai extract (KE) againts TNF-α level in BALB/c mice infected P. berghei ANKA. The research is true experimental study, Posttest-only with Control Group Design. Teatment groups were devided into 4 groups treated with 10 mg/kg BW, 100 mg/kg BW of KE, and 36,4 mg/kg BW artesunate orally (positive control), 3 hours post infection and when parasitemia reached 15-20%. Negative controls were without KE treatment and P. berghei infection. Treatment were given for four days. Blood was collected 24 hours after the last treatment. Plasma TNF-α level were measured by sandwich ELISA. Data was analyzed by using Kruskal-Wallis Test, confidence rate at 95%.  There was a significant different between treatment groups, where p = 0,000 (p < 0,05). KE potential to inhibit TNF-α production in Pb3K100A- group (p = 0,047).Keywords : Malaria,  TNF-α, Stenochlaena palustris Abstrak:  Malaria masih menjadi masalah kesehatan utama pada sebagian besar negara tropis dan subtropis, termasuk Indonesia.  Malaria berat menyebabkan angka kematian yang tinggi meskipun telah mendapat obat anti malaria yang efektif.  Sitokin pro-inflamasi seperti TNF-α meningkat pada malaria berat.  Di Kalimantan Selatan, tanaman kelakai digunakan untuk mengobati demam dan penyakit infeksi.  Kelakai mengandung senyawa-senyawa bioaktif  antara lain flavonoid, steroid, dan alkaloid yang dilaporkan banyak memiliki efek biologis, termasuk aktivitas anti-inflamasi.  Tujuan penelitian ini adalah untuk mengetahui potensi ekstrak kelakai terhadap kadar TNF-α pada mencit BALB/c yang diinfeksi P. berghei ANKA.  Penelitian ini merupakan studi eksperimental murni dengan Posttest-only with Control Group Design.  Kelompok perlakuan dibagi menjadi 4 yaitu kelompok yang mendapat ekstrak kelakai per oral 10 mg/kg BB, 100 mg/kg BB, artesunat 36,4 mg/kg BB (kontrol positif) 3 jam setelah infeksi dan pada saat parasitemia mencapai 15-20%.  Kontrol negatif  tidak mendapat ekstrak kelakai, artesunat, dan infeksi parasit.  Perlakuan diberikan selama 4 hari.  Sampel darah diambil 24 jam setelah perlakuan terakhir.  Kadar TNF-α diukur dengan ELISA metode sandwich.  Data dianalisa dengan Uji Kruskal-Wallis, dengan tingkat kepercayaan 95%.  Terdapat perbedaan bermakna antar kelompok perlakuan, nilai p = 0,000 (p<0,05).  Ekstrak kelakai berpotensi menghambat produksi TNF-α pada kelompok Pb3K100A- (p = 0,047). Kata-kata kunci : Malaria, TNF-α, Stenochlaena palustris
Effects of Areca Catechu L. Seed Extract on Mortality Anopheles Vagus Larvae Mading, Majematang; Kazwaini, Muhammad; Utomo, Budi; Arwati, Heny; Yotopranoto, Subagyo
KEMAS: Jurnal Kesehatan Masyarakat Vol 13, No 3 (2018)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v13i3.11092

Abstract

Bioinsecticide is an alternative to overcome larva resistence to insecticide and reduce environtment contamination. This research is to analyze the effect of areca seed extract to An. vagus larva. The research is taken time in 2017. The research design is laboratory experiment with post test only control group design. The areca seed and An. vagus larva are collected from Kabupaten Sumba Barat Daya, then the seeds were extracted with ultrasonic method. The effect of the extract is evaluated based on LC50 and LC90 value. The sample from 25 An. vagus larva instar III are released into areca seed extract on 500, 1250, 2000, 2750, 3500, 4250 and 5000 ppm and observed within 6, 12, 18 and 24 hours and 6 repetition. The data then analyzed with probit analysis and ANOVA. The areca seed extract eliminates An. vagus larva as much as 61.33% under 6 hours exposure with 5000 ppm, 52% larva mortality on 12 hours with 4250 ppm, 56% larva mortality on 18 hours with 3500 ppm, and 55% larva mortality on 24 hours with 2750 ppm. The value of LC50 and LC90 on 6, 12, 18 and 24 hours are 4654.374 and 6320,732; 3717.286 and 5127.489 ppm; 3201,473 and 4775,206 ppm; 2385,297 and 4496,708 ppm. There is variation of larva mortality percentage average on every concentration and exposure time, the higher the concentration and exposure time the higher An. vagus larva mortality.
Different Types of Anopheles Breeding Place in Low and High Malaria Case Areas Bariyah, Khairul; Utomo, Budi; Sulistyawati, Sulistyawati; Fathmawati, Fathmawati; Supriyanto, Supriyanto; Selviana, Selviana; Arwati, Heny
KEMAS: Jurnal Kesehatan Masyarakat Vol 14, No 2 (2018)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v14i2.14502

Abstract

Malaria is a disease which is transmitted through the bite of Anopheles mosquito. This study aimed to analyse the difference of type of Anopheles larva breeding places between low malaria case area, namely Mandor Village, and high malaria case area, namely North Amboyo Village, both in Landak Regency, West Kalimantan Province. The samples of Anopheles mosquito breeding places was acquired through accidental sampling from all Anopheles larva breeding places and spatial mapping of breeding place points. The data were analyzed using Chi-square test and Mann-whitney test. This study found 70 breeding place points which consisted of 8 types of place, namely dig well, drilled well, Illegal Gold Mining (PETI) well, puddle, fish pond, sewer, swamp, and rice field. The lowest larva density in Mandor Village was at PETI well (0.48/dip) and the highest was at dig well (0.75/dip). In North Amboyo Village, the lowest density was at number 3 dig well (0.2/dip) and the highest was at rice field (2.3/dip). It can be concluded that there was a significant difference of Anopheles breeding places between low and high malaria case areas.
Variety of Anopheles mosquito in Salamwates Village, Dongko Subdistrict Trenggalek District, East Java Province Yuniawan, Farah Shabrina Amazida; Utomo, Budi; Arwati, Heny
KEMAS: Jurnal Kesehatan Masyarakat Vol 15, No 2 (2019)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v15i2.15937

Abstract

Malaria is an infectious disease caused by parasites from the genus Plasmodium and transmitted through the bites of Anopheles mosquitoes. This study was an observational analytic research by using adult mosquito specimens. The mosquitoes were caught using indoor net trapping and outdoor cow baited net trapping started at 18.45 to 24.00 for six catching times. Outdoor cow baited net trapping caught 61 mosquitoes from seven different species namely An. aconitus, An. annularis, An. barbirostris, An. indefinitus, An. kochi, and An. vagus. Human bait trap only trapped one species, An. indefinites. The most often caught mosquito was a zoophilic An. barbirostris, while An. indefinitus was the only spesies of anthropozoophilic mosquito and suspected as the malaria vector because they were caught using indoor human bait trap.
Microscopic-Based Trend in Imported Malaria among Workers of Trenggalek District Returning from Kalimantan Island, Indonesia During 2012 – 2016 Arwati, Heny; Kusmartisnawati, Kusmartisnawati; Yotopranoto, Subagyo; Hidayat, Poppy; Dachlan, Yoes Prijatna
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.14

Abstract

Malaria in Indonesia remains a significant health problem. Trenggalek District is one of the receptive malaria areas in East Java Province, Indonesia, with mainly imported malaria from outside Java Island. The people of this district accustomed to traveling to outside Java where malaria is endemic for temporary working purposes. Usually, they have acquired malaria infection in the areas where they worked and brought to their homeland during their return to the district. The number of imported malaria cases depend on the returning season of the workers. When no workers return from outside Java malaria case is zero. During the year of 2012-2016 microscopic examination of Microscopic examination of Giemsa-stained thick blood films of the workers have been done in the working activity of Pandean Primary Health Center on their arrival from Kalimantan Island either by active or passive case detection. Microscopically positive data were reported herein to describe the trend of imported malaria in the studied areas. A total number of malaria-infected workers were 118 cases. The trend towards increasing case number yearly between 2012 and 2013 being 1.35%, then decreasing between 2013 and 2016 to only 4 cases in 2016. Different patterns of imported malaria by month were seen in each year but no specific pattern throughout the year. The Plasmodium vivax infection was predominant every year and followed by Plasmodium falciparum and mixed infection of those two species. Trends of imported malaria cases in Trenggalek district were decreased and increased irregularly in each month yearly. However, the number of cases declined annually during 2013 – 2016. Antimalarial prophylaxis and insecticides prevent the workers from malaria infection.
Hubungan antara Kadar Prostate Specific Antigen Serum dan Skor Gleason pada Adenokarsinoma Prostat Navisa, Claudia Clary; Sandhika, Willy; Arwati, Heny
Jurnal Kedokteran Brawijaya Vol 30, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2019.030.03.3

Abstract

Kanker prostat adalah kanker terbanyak ke empat di dunia dan merupakan kanker terbanyak ke dua yang menyerang pria. Pada tahun 2013, angka kejadian kanker prostat di Indonesia adalah 25.012 dengan jumlah kasus terbesar yaitu di Jawa Timur sebanyak 5.668. Prostate Specific Antigen (PSA) sebagai penanda tumor, dikatakan berhubungan secara linier dengan skor Gleason. Tujuan dari penelitian ini adalah untuk mengetahui hubungan antara kadar PSA serum dengan skor Gleason pada pasien kanker prostat di RSUD Dr. Soetomo. Penelitian ini merupakan studi analitik dengan desain belah lintang. Data penelitian dikumpulkan dari data rekam medis pasien kanker prostat yang menjalani pemeriksaan kadar PSA serum dan histopatologi jaringan prostat di RSUD Dr. Soetomo Surabaya pada tahun 2015-2017. Pasien dengan Benign Prostate Hyperplasia, infeksi saluran kencing, dan prostatitis dalam 1 bulan terakhir dimasukkan ke dalam kriteria eksklusi. Pada penelitian ini didapatkan 62 sampel. Rentang usia sampel 28-85 tahun dengan proporsi terbanyak pada kelompok usia 58-72 tahun, yaitu sebesar 66,7%. Dari keseluruhan sampel, 41,3% memiliki nilai kadar PSA serum >99ng/dL dan 85,5% memiliki skor Gleason yang buruk. Hasil uji Spearman menunjukkan adanya hubungan yang bermakna (r=0,216; p=0,046) antara kadar PSA serum dan skor Gleason pada pasien kanker prostat di RSUD Dr. Soetomo Surabaya. 
Chemical Composition and In vitro Antibacterial and Cytotoxic Effect of Nigella sativa L. Seed Extract Cyuzuzo, Callixte; Arwati, Heny; Irene, Tuyishimire; Shoukat, Shahzad
KEMAS: Jurnal Kesehatan Masyarakat Vol 16, No 3 (2021)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v16i3.26799

Abstract

Nigella sativa L. is a plant with high medicinal profile in the treatment of diseases. This study aimed to assess the chemical composition and in vitro antibacterial and cytotoxic effect of Nigella sativa L. seed extract. Seeds were purchased from supermarket and blended into powder using electric blender. The powder was macerated with 96% methanol. The extracts were then qualitatively screened to test the presence of secondary metabolites and agar well diffusion method was employed to study the antibacterial activity of extracts. In this evaluation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to examine the cytotoxic effect of extract on peripheral blood mononuclear cells (PBMCs). MTT assay was done on different extract concentrations for 3 days and the results were recorded daily. The findings revealed the presence of tannins, terpenoids, steroids, saponins, alkaloids, phenolic compounds and flavonoids. The antibacterial activity was observed with the inhibition zones ranging from 11.3 ±1.1 to 16.3 ±1.5mm while the minimum inhibitory concentration (MIC) values of extract ranged between 0.5 and 1.25 μg/mL. The black seed extracts contain bioactive substances with germicidal activity and in vitro administration of black seed extract up to the dose of 100 μg/mL resulted no cytotoxic effect on PBMCs.
Qualitative Phytochemical ScreeningandIn VitroAntibacterialActivitiesof Crude Akaziraruguma Leaf Extracts Cyuzuzo Callixte; Heny Arwati; Dusabimana Jean Damascene
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16833

Abstract

The resistance of pathogenic bacteria is a global health dynamic and the time is nowtofind alternativesolutions from plant secondary metabolites.Ageratum conyzoidesL. is a plant known as akazirarugumain Rwandaand used to treat wounds and ulcers. This study aimedto evaluate the phytochemical profileand germicidal efficacy of ethanolic and aqueous leaf extracts of Ageratum conyzoides L. grown inRwasave wetland.Theleaves were collected and dried under the shed for 10 days, blended into powderby electric blender and macerated with water and 96% ethanol. Phytochemical screening wasperformedby followingthe standard procedures and antibacterial activity of the extracts wasexamined by agar welldiffusion method and the inhibition zones were recorded.Phytochemical screening revealed the presence ofdifferent secondary metabolites including alkaloids, tannins, flavonoids, steroids, terpenoids and saponins. In this assessment,aqueous and ethanolic extracts exhibited significant inhibitory activity against testedpathogens with inhibition zones that ranging from6±0.9 mm to 20±0.5 mm of diameter. The minimuminhibition concentrations range between 0.47 mg/mL and 15.00 mg/mL.The resultsconfirm that the leavesof akazirarugumacould be the credible source ofantibacterial agents that should be used for therapeuticpurposes and in production of pharmaceuticals.
Sensitivity and Specificity of Rapid Diagnostic Test with Microscopic Gold Standard to Identify Plasmodium Species Sahrir Sillehu; Heny Arwati; Yoes Prijatna Dachlan; Sudjajadi Keman
International Journal of Public Health Science (IJPHS) Vol 5, No 4: December 2016
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.104 KB) | DOI: 10.11591/ijphs.v5i4.4829

Abstract

Malaria is a main health problem in islands area which is under developed and isolated. Nation-wide, in 2014 Maluku province was recorded to have Annual Malaria Incident (AMI) value of 30.4%, positive incidents of 13.30%, ABER 3.76%, SPR 21.50%, and Annual Paracite Incident (API) 8.10%, while South Buru Regency has a value of Annual Malaria Incident (AMI) of 14.49%, 494 positive incidents, ABER 1.12%, SPR 60.91%, and Annual Paracite Incident (API) 6.86%. The purpose of this study was to identify Plasmodium species in malaria incidents in NamroleSubdistrict, South Buru Regency, Maluku Province. Observational research with a sample of 64 respondents for symptomatic and asymptomatic malaria. The instrument for the research was Rapid Diagnostic Test (RDT) and microscopic Gold Standard. Result: Malaria examination by using RDT suggested 3 kinds of parasites, i.e., P. falciparum, P. Vivax, and a mix between P. falciparum and P. vivax. Most parasites found were P. falciparum 56.3%. The accuracy of RDT examination was proven with microscopic test and the result suggested that the RDT sensitivity was 100% and the specifivity was 63.3%. Positive predictive value was 92.9% and negative predictive value was 100%, both were for positive likelihood ration of 2.75%. While for negative likelihood ration of 0%, the value of degree of conformity (Kappa) between RDT and microscopic is 0%. RDT has one benefit that it can be use to conduct malaria diagnosis rapidly, particularly in isolated areas. The benefit of Rapid Diagnostic Test (RDT) was that it could be used in remote and isolated areas to conduct diagnosis. RDT is highly effective and efficient.
Pengaruh Ekstrak Kelakai (Stenochlaena palustris (Burm.f) Bedd) terhadap Kadar Interleukin-10 (IL-10) Mencit Denny Margono; Eko Suhartono; Heny Arwati
Medical Laboratory Technology Journal Vol. 2 No. 1 (2016): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.006 KB) | DOI: 10.31964/mltj.v2i1.43

Abstract

Abstract : Interleukin-10 as pro-inflammatory cytokines have a role to protect the damage of severe malaria. Kelakai contain bioactive compounds that have anti-inflammatory activity. The purpose of this study was to determine the potential of the extract kelakai on levels of IL-10 in mice BALB / s infected by P. berghei ANKA. This study is true experimental studies with posttest-only with Control Group Design. The treatment group was divided into 8 groups. Two groups received kelakai extract per oral 10 mg/kgBW and 100 mg/kgBW. Four groups received kelakai extract per oral 10 mg/kgBW and 100 mg/kgBW 3 hours after infection and when the parasitaemia reaches 15-20%. Negative controls do not receive the extract kelakai and parasitic infections. Positive controls get a parasitic infection. Treatment was given for 4 days. Blood samples were taken 24 hours after the last treatment. Levels of IL-10 were measured by sandwich ELISA method. Data were analyzed with Games Howell test, with a confidence level of 95%. There were no significant differences between treatment groups. Extract kelakai Giving oral dose of 10 mg/KgBW and 100 mg/KgBW not significantly increase the levels of interleukin-10. Keywords: Stenochlaena palustris (Burm.f) bedd; Interleukin-10 Abstrak : Interleukin-10 sebagai Sitokin pro-inflamasi memiliki peran melindungi kerusakan pada malaria berat. Kelakai mengandung senyawa bioaktif yang mempunyai aktifitas anti-inflamasi. Tujuan penelitian ini adalah untuk mengetahui potensi ekstrak kelakai terhadap kadar IL-10 pada mencit BALB/s yang diinfeksi P. berghei ANKA. Penelitian ini merupakan studi eksperimental murni dengan Posttest-only with Control Group Design. Kelompok perlakuan dibagi menjadi 8 kelompok. Dua kelompok mendapat ekstrak kelakai per oral 10 mg/kg berat badan dan 100 mg/kg berat badan. Empat kelompok mendapat ekstrak kelakai per oral 10 mg/kg berat badan dan 100 mg/kg berat badan 3 jam setelah infeksi dan pada saat parasitemia mencapai 15-20%. Kontrol negatif tidak mendapat ekstrak kelakai dan infeksi parasit. Kontrol positif mendapat infeksi parasit. Perlakuan diberikan selama 4 hari. Sampel darah diambil 24 jam setelah perlakuan terakhir. Kadar Il-10 diukur dengan ELISA metode sandwich. Data dianalisa dengan tes Games Howell, dengan tingkat kepercayaan 95%. Tidak didapatkan perbedaan bermakna antar kelompok perlakuan. Pemberian Ekstrak kelakai secara oral dosis 10 mg/Kg berat badan dan 100 mg/Kg berat badan tidak signifikan meningkatkan kadar Interleukin-10. Kata-kata Kunci : Stenochlaena palustris (Burm.f) Bedd; Interleukin-10
Co-Authors Amadeus Michel Goein Anak Agung Istri Sri Wiadnyani Apsari, Putu Indah Budi Aryati Aryati Awwaliyah Azmi Izzati Bagus Dwi Nugraha BUDI UTOMO Budi Utomo Budi Utomo Cyuzuzo Callixte Cyuzuzo Callixte Cyuzuzo Callixte Cyuzuzo, Callixte Denny Margono Denny P.N.H. Margono Denny P.N.H. Margono, Denny P.N.H. Dewi Hariyani Dinda Eka Putri Dusabimana Jean Damascene DWI PENI KARTIKASARI DWI SUSANTI Dyah Ayu Febiyanti Eka Noviya Fuzianingsih Eko Suhartono Eko Suhartono Eko Suhartono Fathmawati Fathmawati Florentina Sustini Frista Amalia Hartono Kahar Hartono Kahar, Hartono Hebert Adrianto HERU PRASETYO Hidayat, Poppy Inas Hasna Azizah Indah S Tantular Indah Setyawati Tantular, Indah Setyawati INDAH TANTULAR Irene, Tuyishimire IVAN RAHMATULLAH Jelita Aprisano Putri Juniastuti Juniastuti Kartika Arum Wardani KARTIKASARI, DWI PENI Khairul Bariyah Khairul Bariyah, Khairul Kholida Nur Aini Kris Cahyo Mulyatno Kusmartisnawati Kusmartisnawati Kusmartisnawati Kusmartisnawati Kusmartisnawati Kusmartisnawati, Kusmartisnawati Laily Nur Azizah Lelyana Sih Afgriyuspita Lindarto, Wira Widjaya Lucia Tri Suwanti, Lucia Tri LYNDA ROSSYANTI M. Yasin Fitri Nugroho Majematang Mading Mardliyana Shalihah Marselaonety La’lang Meliance Bria Mufassirin Mufassirin Muhammad Kazwaini Muhammad Zulkifly Tasman Nabilla Feirizky Chairunnisa Nathania Disa Ariesta Andriani Navisa, Claudia Clary Ni Wayan Winianti Nindya Tresiana Putri Nsanzimana Jean Baptiste Nurdianto, Arif Rahman Nurina Hasanatuludhhiyah Poppy Hidayat Priangga Adi Wiratama Puspa Wardhani Puspa Wardhani Puspa Wardhani Putri Rahayu Putu Indah Budi Apsari putu indah budi apsari Raudatul Jannah Rosalia Friska Ananda ROSSYANTI, LYNDA Sahrir Sillehu Selviana Selviana Shoukat, Shahzad Siti Munawaroh SRI HIDAJATI Sri Subekti SRI WIJAYANTI SULISTYAWATI Stevani Florentia Bahi SUBAGYO YOTOPRANOTO Subagyo Yotopranoto Subagyo Yotopranoto Subagyo Yotopranoto Subagyo Yotopranoto Sudjajadi Keman Suhintam Pusarawati Sukmawati Basuki Sulistyawati, Sriwijayanti Suprapto Maat Supriyanto Supriyanto SUSANTI, DWI Syafarinah Nur Hidayah Akil Tantular, Indah Tanzilia, May Fanny Thomas Tandi Manu Willy Sandhika Windya Tri Hapsari Wiwied Ekasari Yetti Hernaningsih Yoes Prijatna Dachlan Yuniawan, Farah Shabrina Amazida Yuwono Marta Dinata