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All Journal Indonesian Journal of Tropical and Infectious Disease Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology
Puspa Wardhani Puspa Wardhani
Stmik Jakarta
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Medicine & Pharmacology Neuroscience Public Health

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Profile Quantitative Hepatitis B Surface Antigen (qHBsAg) of Chronic Nai?ve Hepatitis B Patients in Dr. Soetomo Hospital, Surabaya, Indonesia Yessy Puspitasari; Puspa Wardhani; Munawaroh Fitriyah; Erik Hasudungan; Atika; Ummi Maimunah; Aryati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14941

Abstract

This study aimed to evaluate the profile of qHBs Ag profile, and also to investigate the correlation betweenqHBs Ag and HBV DNA. Seventy samples of chronic-nai?ve hepatitis B patients in Dr. Soetomo Hospitalwere analyzed in a cross-sectional study. Patients were categorized according to the HBe Ag positive (n=30)and HBe Ag negative (n=18), also based on qHBs Ag 1000 IU/mL and qHBs Ag >1000 IU/mL. qHBs Agwas correlated with HBV DNA. qHBs Ag by CLEIA method from Sysmex, KOBE HISCL, HBV DNAwas measured by real-time Polymerase Chain method from Gene Xpert, Cepheid. 70 patients nai?ve CHBtreatment showed a median of ALT level 60.21±70.76 U/L. 30 patients showed a positive-HBeAg, 18 patientsshowed negative-HBeAg, 22 patients were not evaluated (N/A). Positive-HBeAg patients had 70% qHBsAg>2500 mg/dL and median HBV DNA 7.49×107IU/mL. Negative-HBeAg patients had 55.6% HBsAg ?1000mg/dL and median HBV DNA 9.66×102 IU/mL. qHBsAg correlated with HBV DNA (p <0.001). This datademonstrated that quantitative HBsAg was associated with a phase of HBV-infection, quantitative HBsAgshowed a moderate correlation with DNA HBV, quantitative HBsAg levels might be a predictor of initiationtherapy for CHB patients.
Nephroprotective effect of virgin coconut oil in Plasmodium berghei ANKA infected Balb/c mice Syafarinah Nur Hidayah Akil; Heny Arwati; Puspa Wardhani; Priangga Adi Wiratama
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 5, No 2 (2021)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v5i2.5791

Abstract

AbstractMalaria is a parasitic infectious disease caused by Plasmodium, which remains a world health problem with an estimated 219 million cases worldwide. In severe malaria infection, several organs of the body can be affected, including the kidneys. One of the pathophysiology associated with the worsening of this disease is oxidative stress. The use of antioxidants is expected to prevent this, and one product that has a high antioxidant content is virgin coconut oil (VCO). This study aimed to analyze the effect of VCO on the kidney in Plasmodium berghei ANKA-infected mice. This study was an in vivo laboratory experimental study with a randomized post-test only control group design using 35 BALB/c mice infected with P. berghei ANKA, weighing 20-30 grams. VCO with the Javara® brand is used with doses of 1, 5, and 10 ml/kg body weight (kgBW)/ day. The parameter assessed were levels of BUN, creatinine, and renal histopathological changes. The administration of VCO on the treated group shows minimal tubular necrosis and glomerulonephritis compared to the negative control group. The BUN and creatinine levels in the treated group were also lower than the negative control group. The results showed that VCO has a nephroprotective effect against P. berghei ANKA infection in mice.Keywords: malaria, kidney, virgin coconut oil
Contamination of water and soil of rice fields with soil transmitted helminths as source of transmission to farmers in Grogol sub-district, Kediri district Siti Munawaroh; Heny Arwati; Puspa Wardhani
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 4, No 1 (2020)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v4i1.3402

Abstract

ABSTRACTSoil-transmitted helminths (STH) are the worms which transmitted through the soil. The people of Dusun Semen, Grogol, Kediri have a habit of defecating in the river. Farmers who work in direct contact with water and soil is possible to be infected with STH. The purpose of this study was to determine STH species in water, soils, and farmer's fecal samples. Water samples were collected from three spots of river A and B, soil samples from 43 rice fields, and fecal samples from 50 farmers. Water samples were examined by sedimentation, soil samples by floatation, and fecal samples by Kato-Katz method. The data were then analyzed by Chi square test. Out of 18 water samples, 12 samples (66.7%) were positive, consisted of 4 samples (22.2%) contained of Ascaris lumbricoides and 8 samples (44.4%) contained Trichuris trichiura. Total soil samples were 129, where 8 samples (6.2%) were positive for A. lumbricoides, and 10 samples (7.7%) for T. trichiura. Out of 50 villagers, only 39 gave the fecal samples, where 12 samples (31.0%) were positive consisted of 5 samples (12.8%) for A. lumbricoides, 8 samples (20.5%) for T.trichiura. In conclusion, the STH species found in the media of water, soil, and feces of farmers are the similar namely A. lumbricoides and T. trichiura. Positive farmer samples prove STH transmission from STH contaminated water and soil.Keyword: Water, soil, feces, soil transmitted helminths
RNA ISOLATION OF DENGUE VIRUS TYPE 1 WITH DIFFERENT PRECIPITATION SOLVENTS: DIMETHYL SULFOXIDE, ACETONE, AND ETHANOL 70% Anisa Maharani; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Shuhai Ueda; Soegeng Soegijanto
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 3 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.948 KB) | DOI: 10.20473/ijtid.v7i3.6748

Abstract

Dengue Hemorrhagic Fever (DHF) is caused by dengue viruses that belong to Flaviviridae. The disease is known to be caused by 4 types of dengue viruses, namely DENV-1, DENV-2, DENV-3, and DENV-4 associated with antigenic. Dengue virus is a virus RNA that causes illness with clinical manifestations of Dengue Fever, Dengue Hemorrhagic Fever and Dengue Shock Syndrome. The aim of research was to determine the effectiveness of dimethyl sulfoxide, acetone, and ethanol 70% as precipitation solvent in the process of RNA isolation. The method used was Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) with specific primers for dengue virus type 1 (DENV-1). RNA isolation can be done easily using an RNA Isolation Kit. Use of RNA Isolation Kit results in a purer RNA isolate from contaminants and from RNA degradation. In generally the isolation is using cold ethanol / alcohol with concentration 90-95%. Ethanol / Alcohol does not dissolve RNA and light density of alcohol lighter than water makes RNA rise and hover on the surface. In RNA isolation solvent precipitation that used are acetone, ethanol 70%, and DMSO. In qualitative RNA measurements using agarose gel electrophoresis and was examined under the UV light-illuminator and quantitative RNA measurements using Nanodrop spectrophotometry with absorbance ratio at 260/280 and 260/230 showed a good result indicated by the appearance of the band on electrophoresis results in PCR. While the measurement quantitatively is showed that there was still protein contamination but the results are quite good because it does not much different from the ratio set in the reference. Acetone, ethanol 70%, and DMSO can be used as a substitute of 96% ethanol in the process of RNA isolation in DENV-1 virus and can also be applied to other dengue virus because the structure of the 4th antigen serotype is very similar one with the other and no effect.
DETEKSI ANTI GLUTAMIC ACID DECARBOXILASE/TYROSINE PHOSPHATASE (ANTI GAD/IA2) PADA PENDERITA DM TIPE 1 ANAK Puspa Wardhani; S Darmadi; M Faizi; Netty Harjantien
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 2 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i2.907

Abstract

Our study evaluated anti GAD/IA2 levels in Type 1 DM patients in Dr. Soetomo Hospital Surabaya. We conducted cross sectionalstudy, involving Type 1 DM patients that already been established (C-Peptide bellow normal). Patient’s age range was from 2.8 yearsold to 17 years old, so they were regarded as children. We used anti GAD/IA2 reagents from Euroimmun wich had basic principal EIA.Twelve patients was involved in this study. The average level of anti GAD/IA2 was 210.37 IU/mL. The Average level of anti GAD/IA2was higher in female than male patients (227.38 IU/mL dan 162 IU/mL). All patients had anti GAD/IA2. This examination can beusefull for screenimg one who has high risk in developing type 1A DM, especially first degree relatives.
ANALYSIS OF DENGUE SPECIFIC IMMUNE RESPONSE BASED ON SEROTYPE, TYPE AND SEVERITY OF DENGUE INFECTION Ade Rochaeni; Aryati Aryati; Puspa Wardhani; Usman Hadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1199

Abstract

Infeksi Virus Dengue (IVD) menimbulkan derajat klinis beragam dari DD hingga DBD/SSD. Respons imun spesifik dengue berupaIgM dan IgG anti dengue masih merupakan perdebatan untuk patogenesis DBD di samping faktor virulensi virus dan jenis infeksi.Penelitian ini bertujuan untuk menganalisis respons imun spesifik dengue terhadap serotipe, jenis dan derajat IVD di Surabaya. Subjekadalah pasien IVD yang dirawat di Ruang Tropik Infeksi Penyakit Dalam RSUD Dr. Soetomo dengan hasil penyaringan uji cepat NS1 (SDBioline Dengue Duo) dan/atau PCR (Simplexa Dengue) positif. Pemeriksaan IgM dan IgG anti dengue kuantitatif dengan metode ELISA(Panbio Dengue Duo IgM and IgG Capture). Penelitian dilakukan Maret–Agustus 2016 dan didapatkan 61 pasien dengan hasil NSI dan/atau PCR dengue positif. Identifikasi serotipe didominasi DEN-3, namun serotipe yang lebih virulen ditunjukkan DEN-1 yaitu semuapasien bermanifestasi sebagai infeksi sekunder dan DBD. Jenis infeksi primer sebanyak 19 (31,1%) dan infeksi sekunder 42 (68,9%).Derajat IVD meliputi DD 10 (16,4%), DBD 47 (77%) dan SSD 4 (6,56%). Nilai indeks rerata IgM dan IgG anti dengue di kelompokinfeksi serotipe DEN-1 (5,140 dan 5,774), DEN-2 (2,971 dan 2,222), DEN-3 (1,863 dan 2,792); kelompok jenis infeksi primer (1,478 dan0,746), sekunder (4,028 dan 4,864) dan kelompok derajat DD (1,170 dan 1,492), DBD I (3,370 dan 3,651), DBD II (3,924 dan 4,439)dan DBD III (4,164 dan 4,243). Sebagai simpulan respons imun spesifik dengue didapatkan lebih tinggi bermakna di kelompok infeksiserotipe DEN-1, kelompok jenis infeksi sekunder dan kelompok DBD/SSD.
PROFIL VIRUS DENGUE DI SURABAYA TAHUN 2008–2009 aryati .; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1046

Abstract

Four serotypes of dengue viruses (DENV) 1–4 are mosquito-borne human pathogens that cause widespread epidemics withconsiderable morbidity and mortality. The aim of this study was to evaluate the dengue serotypes profile, which were circulating inSurabaya. This research has been carried out consisting of 360 samples from patients with dengue virus infections, according the WorldHealth Organization (WHO) criteria. These sera were collected from patients Dr. Soetomo Hospital and private laboratory in Surabayafrom 2008–2009. From 360 samples, 68 samples (18.9%) were undifferentiated fever, 53 samples (14.7%) were dengue fever, 239samples (66.4%) were dengue hemorrhagic fever. From 58 Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) samples, 25samples (43%) were positive, consisting of 52% DEN-2, 20% DEN-1, 16% DEN-3 and 12% DEN-4. These results showed that fourserotypes are circulating in Indonesia, dominated by DEN-2, followed by DEN-1, DEN-3 and DEN-4.
NILAI DIAGNOSTIK ANTI DENGUE IgA DAN NS1, SERTA IgM/IgG DI INFEKSI VIRUS DENGUE (The Diagnostic Value of Anti Dengue IgA and Anti Dengue IgM/IgG in Dengue Virus Infection) Resna Resna; Aryati Aryati; Puspa Wardhani; Erwin Triyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1264

Abstract

The clinical manifestations of dengue virus infection are varied and thus a specific diagnostic examination is required. Usually antidengueIgM is often used, but the presence in the circulation is 3−8 months long. NS1 is sensitive in the detection of primary infection,whereas IgG is more better used in secondary infection. The examination of anti-dengue IgA as a new marker is estimated to be ableto detect the acute primary and secondary infection, however the diagnostic value of anti-dengue IgA is not much well known for theIndonesian population. This study was done at the Tropical Infectious Disease Ward of Dr. Soetomo Hospital, Surabaya during February– April 2013. The samples consisted of 37 sera from patients infected by dengue virus and 37 sera from those non one (dengue virusinfection patients). The NS1 serum, anti-dengue IgM and anti dengue IgG were examined by ELISA and anti-dengue IgA was examined byan indirect immunochromatography method using Assure@ Dengue IgA Rapid Test (MP Biomedicals Asia Pacific Pte Ltd). The diagnosticvalue was analyzed by 2x2 table with a confidence interval of (CI) 95%. The used gold standards were from the 1997th WHO criteriaand one of the positive dengue serological tests by ELISA (NS1/anti dengue IgM/anti dengue IgG). AUC and anti-dengue IgA cut-off weredetermined by ROC curve. The Diagnostic value of anti-dengue IgA showed a sensitivity and specificity of 83.8% (67.3 to 93.2) and 81.1%(64.3 to 91.4). A positive predictive value of 81.6% (65.1 to 91.7) and a negative predictive value of 83.3% (66.5 to 93.0) was found. Thepositive likelihood ratio was 4.4 times (2.2 to 8.8) and negative likelihood ratio of only 0.2 times (0.09 to 0.42). The best cut off valueof 0.2 was shown by the area under the curve of 83.5%. Based on this study, the diagnostic value of anti-dengue IgA had a good validityfor the diagnosis of dengue virus infection.
KEMAMPUAN UJI TABUNG WIDAL MENGGUNAKAN ANTIGEN IMPORT DAN ANTIGEN LOKAL Puspa Wardhani; Prihatini Prihatini; Probohoesodo M.Y
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 1 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i1.838

Abstract

Despite of its higher specificity than Widal-slide test, Widal-tube test is not widely used by medical laboratories because it is not practical and each laboratory has to produce their own antigens. The Laboratory must have sufficient microbiology equipments and reagents to produce antigens. REMEL® provides ‘ready for use’ antigens to perform Widal-tube test. To Compare and correlate Widal-tube test using REMEL® imported antigens and local antigens (produced by lab. Clinical pathology Dr. Soetomo hospital). The samples were tested by Widal-tube test, using REMEL® and local antigens, comparing Salmonella typhi (ST) O antigen, ST H antigen, S. paratyphi (SP) A H antigen, and SP B H antigen for each method, with twofold dilution from 1/40 until 1/1280. The number of samples was 55. The results are defined as pathologic (above cut-off value) and non pathologic (below cut-off value). REMEL® ST O antigen had a significant correlation to local antigens (r = 0.665, p < 0.01). REMEL® ST H antigen also had a significant correlation to local antigen (r = 0.671, p < 0.01), REMEL® SP B H has no significant correlation to local antigen (r = 0.389, p < 0.01). All samples (55) showed negative results (non pathologic) using SPA H local antigen. When using REMEL® SPA H antigen, 51 were non pathologic and 4 were pathologic. Widal-tube test using REMEL® antigens has significant correlation with local antigens so it might be considered to be used for diagnosing typhoid fever.
KETERKAITAN ANTIGEN NS1 INFEKSI VIRUS DENGUE DENGAN SEROTIPE VIRUS DENGUE Roudhotul Ismaillya Noor; Aryati Aryati; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1005

Abstract

Dengue virus infection (DVI) currently is detected by using dengue virus NS1 antigen (NS1 Ag). The sensitivity of NS1 Ag is 27.8%–93.4%,but recent study of Kumarasamy the sensitivity of NS1 Ag is better than the virus isolation and polymerase chain reaction (PCR). This studyis focussed on the evaluation of the validity of Panbio Dengue Early Rapid for the diagnosis of DVI and the NS1 Ag sensitivity associated withdengue virus serotypes. The sera was obtained from 65 DVI patients which diagnosed by the clinicians. The resulted diagnosis was foundby serology tests (positive IgM/IgG antidengue/NS1 Ag ELISA) and 1997 WHO criteria as the gold standard, and which also found 35 nonDVI patients (typhoid fever, HAV, malaria, UTI, tuberculosis and bronchopneumonia). The samples were examined by Panbio Dengue EarlyRapid. PCR was performed on each positive serological test result to determine the dengue virus serotypes. The sensitivity and specificity ofPanbio Dengue Early Rapid was 49.2% and 100%. The PCR results of 65 sera showed positive PCR in 49.2% (positive NS1 Ag was 62.5%).Meanwhile, and negative PCR in 50.8% (positive NS1 Ag was 36.4%). The predominance of serotypes (positive NS1 Ag) were DEN-3 (37.5%),DEN-4 (28.1%), DEN-1 (21.9%) and DEN-2 (12.5%). The Panbio Dengue Early Rapid can be used as early detection of DVI, although itshould be used in conjunction with other dengue serological tests as well. Unfortunately there is still not enough evidence about the NS1 Agsensitivity associated with the dengue virus serotypes.
Co-Authors Ade Rochaeni Ahmad Yudianto Anisa Maharani Aryati aryati . Aryati Aryati atika Erik Hasudungan Erwin Triyono Fery H Soedewo Harsasi Setyawati Henny Elfira Yanti Heny Arwati IG Eka Sugiartha IGAA Putri Sri Rejeki Ilham Harlan Amarullah Izzuki Muhashonah Jeine Stela Akualing Juli Soemarsono M Faizi Muhammad Zulkifly Tasman Munawaroh Fitriyah Netty Harjantien Nurina Hasanatuludhhiyah Priangga Adi Wiratama Prihatini Prihatini Probohoesodo M.Y Puspitasari, Yessy Resna Resna Rosalinda Avia Eryatma Roudhotul Ismaillya Noor S Darmadi Semedi, Bambang Pujo Shuhai Ueda Siti Churrotin Siti Munawaroh Soegeng Soegijanto Syafarinah Nur Hidayah Akil Teguh Hari Sucipto Ummi Maimunah Usman Hadi