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All Journal International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Althea Medical Journal Medical Journal of Indonesia Indonesian Journal of Biotechnology Global Medical and Health Communication The Indonesian Biomedical Journal Jurnal Sistem Kesehatan Universa Medicina Paediatrica Indonesiana GIZI INDONESIA Journal of Epidemiology and Public Health The Indonesian Journal of Infectious Diseases Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Tunas Medika Jurnal Kedokteran & Kesehatan Jurnal Penyakit Dalam Indonesia
Bachti Alisjahbana
Department Of Internal Medicine Faculty Of Medicine Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Implementation of 25-well culture plates for M. tuberculosis drug susceptibility testing in Indonesia Rosana, Yeva; Sudiro, Tjahjani M.; Alisjahbana, Bachti; Parwati, Ida; van Crevel, Reinout; van Soolingen, Dick
Medical Journal of Indonesia Vol 14, No 3 (2005): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.775 KB) | DOI: 10.13181/mji.v14i3.186

Abstract

At present, there is no standardized method for Mycobacterium tuberculosis drug susceptibility testing (DST) among laboratories in Indonesia. Since January 2001 to January 2004 we have tried to establish the method of 25-well culture plates with middlebrook’s media (Drug Susceptibility Culture Plate (DSCP) method) used by the Dutch Supranational Reference Laboratory at the Institute of Public Health and the Environment (RIVM), Bilthoven, Netherlands. Our experience showed that this method potentially gives better result as it can be very well standardized, faster and provides detailed MIC (Minimal Inhibitory Concentration) values. Data from 364 isolates that have been tested by DSCP method showed that resistance to INH, rifampicin, ethambutol, and streptomycin were 21.4%, 19.8%, 15.7%, and 16.5% respectively. Multidrug resistance were found in 13.2% isolates. (Med J Indones 2005; 14: 142-6)Keywords: M. tuberculosis, DST, DSCP method
EFEK PEMBERIAN SUPLEMEN SINBIOTIK DAN ZAT GIZI MIKRO (VITAMIN A DAN ZINC) TERHADAP STATUS GIZI PENDERITA TBC PARU ORANG DEWASA YANG MENGALAMI KEKURANGAN ENERGI KRONIK Hardinsyah, Suparman; Kusharto, Clara M; Sulaeman, Ahmad; Alisjahbana, Bachti
GIZI INDONESIA Vol 34, No 1 (2011): Maret 2011
Publisher : PERSATUAN AHLI GIZI INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36457/gizindo.v34i1.99

Abstract

Pasien  TB  paru  pada  akhirnya  akan  mengalami  keadaan gizi  buruk  dan  menurunnya  respon  imun. Kemoterapi  dengan  menggunakan  obat-obatan  TB  merupakan  langkah  yang  efektif  untuk  mengobati penyakit ini, tetapi mempunyai pengaruh negatif terhadap keseimbangan mikropola usus inflamasi karena infeksi TB paru, menurunkan pengaturan sintesa zat  gizi dan menurunkan nafsu makan, sehingga terjadi kekurangan  gizi.  Penelitian  bertujuan  untuk  menganalisa  efikasi  symbiotik  dan  suplemen zat  gizi  mikro terhadap status gizi pada pasien dewasa TB paru yang sedang diobati yang menderita kekurangan energi kronik  setelah  2  bulan  menjalani  intervensi.  Desain penelitian  “a  double-blind  randomized  treatment control trial“ dilakukan pada 2 pusat penyembuhan TB paru di Bandung dan Garut. Sejumlah 43 orang yang  menderita  kekurangan  energi  kronik  (KEK)  dipilih  dari  76  pasien  TB  paru  yang  terekrut  dengan variasi umur antara 20–45 tahun. Kelompok ini dibagi 2 kelompok, kelompok pertama diberikan susu yang berisi  protein,  symbiotik  dan  suplemen  micronutrien (MSM)  dan  kelompok  kedua  diberi  susu  yang berbasis protein saja (MO) sebagai kelompok kontrol. Seluruh pasien menerima terapi standar untuk TB paru, parameter status gizi (BB, IMT, masa Lemak, hemoglobin, serum vitamin A dan seng) dikumpulkan pada saat awal setelah 1 bulan, 2 bulan dari pemberian intervensi data di awalnya. Perbedaan antara dan dalam kelompok menggunakan statistik parametrik dannon parametric. Hasil menunjukan bahwa setiap kelompok  pada  akhir  intervensi  parameter  status  gizi  secara  signifikan  mengalami  perbaikan dibandingkan  pada  awal  penelitian  (P0,05)  tetapi  tidak  ada  perbedaan  signifikan  antara  dua  kelompok (p0,05). Ada pengaruh potensial dari setiap suplemen dalam meningkatkan status gizi, penelitian yang lebih lama dengan tidak memberikan susu berbasis protein pada kelompok kontrol dibutuhkan. Kata kunci: imunitas, zat gizi mikro, symbiotik, TBparu
Treatment Seeking Patterns among Dengue Fever Patients: A Qualitative Study Krisnian, Tharani; Alisjahbana, Bachti; Afriandi, Irvan
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.718 KB)

Abstract

Background: Incidence of dengue fever is increasing drastically and has become a major public health problem globally. The reason patients are late in seeking medical treatment should be identified in order to prevent complications which can be avoided to produce a good prognosis. This study was conducted to find the pattern of treatment seeking behaviour among dengue fever patients and their influencing factors using a health utilisation model. Methods: Data on health seeking behaviour were collected among the dengue fever patients who were admitted to Dr. Hasan Sadikin General Hospital Bandung from September–October 2014. The data were collected through in-depth interview with patients who were diagnosed with dengue fever and dengue haemorrhagic fever. All the interviews were recorded using an audio recorder. The recordings were transcribed and then translated into English and analyzed using thematic analysis.Results: Nine patients were interviewed. The age of the patients ranged between 17 to 46 years.  Altogether 7 patterns were identified with patients treatment seeking behavior. Most of them took longer steps to reach adequate care with blood examination. These longer steps, caused by lack of facilities to perform blood test in primary health care facilities, no bed for admission and also low skills of health care providers in diagnosing patients. The primary health care facility played an important role in the delay of patients acquiring definite care for their dengue fever.Conclusions: Health care seeking behavior is hampered by the inadequacy of primary health care facilities to provide adequate services to dengue patients. 
Knowledge, Attitude and Behavior Related to Antibiotic Use in Community Dwellings Parse, Rocci Jack; Hidayat, Eva Mardiana; Alisjahbana, Bachti
Althea Medical Journal Vol 4, No 2 (2017)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.303 KB) | DOI: 10.15850/amj.v4n2.1082

Abstract

Background: Irrational antibiotic use is the major reason for insidence of antibiotic resistance. Antibiotic misuse in community is influenced by environmental and behavioral factors. Environment included community preference for purchasing antibiotics, lack of health care infrastructure, lack of general practitioner and policy in regulating  antibiotic use. Behavior is influenced by lack of information in antibiotic use and unawareness of antibiotic resistancy. The aim of the study was to assess the level of knowledge, attitude and behavior regarding antibiotic use in community dwellings.Methods: A cross sectional descriptive study was carried out to 96 respondents who were selected by a rapid survey sampling method in Cileles village Jatinangor, Sumedang, West Java, Indonesia in August 2013. Data were collected using questionnaires. The data was analyzed in the form of frequency and percentage.Results: Out of 96 respondents, only 40.6% had good knowledge regarding antibiotic use, 12.5% of respondents were prescribed antibiotics, but in the last course did not purchased all the antibiotics prescribed by the clinician and 55.2% of respondents did not complete their treatment course.Conclusions: Most of the community still have poor knowledge regarding antibiotic use and its consequences such as allergy, and resistancy based on the knowledge questions has a total score ≤6. Their attitude and behavior regarding antibiotic use are still poor which is also based on  incompletion of the antibiotic treatment and irregular use.DOI: 10.15850/amj.v4n2.1082
Level of Knowledge about Tuberculosis among Type 2 Diabetes Mellitus Patients at the Endocrine Clinic Dr. Hasan Sadikin General Hospital Bandung Febriani, Ulfah Dwi; Ruslami, Rovina; Alisjahbana, Bachti
Althea Medical Journal Vol 4, No 4 (2017)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.891 KB) | DOI: 10.15850/amj.v49n4.1260

Abstract

Background: Diabetes Mellitus (DM) is one of the risk factors for tuberculosis (TB). Knowledge of TB is essential in prevention control for the person at risk. This study aimed to describe the level of knowledge about TB among DM patients.Methods: A cross sectional study was performed involving 72 adult DM patients at the Endocrine Clinic, Dr. Hasan Sadikin General Hospital Bandung. The subjects were interviewed using a standardized questionnaire including socio-demographic information and knowledge about TB: manifestation of TB, transmission, treatment, DM-TB co-infections, and status acquiring TB information in the last 12 months. Knowledge was categorized as high, moderate and low if total scores were >75, 56–75 and <56 respectively. Data were presented descriptively as percentage and mean (SD) after testing for the normality of distribution.Results: The mean of age of subjects was 56.6 years, and 55% were female. Most were unemployed (72.2%), had an education background lower than high school (75%), and lived in Bandung (91.7%).  The mean score of knowledge was 54.4. Proportion of subjects based on the level of knowledge which is high, moderate and low, were 29.2%, 26.4%, and 44.4% respectively.  Conclusions: The level of knowledge about TB among Type 2 DM patients at the Endocrine Clinic Dr. Hasan Sadikin General Hospital Bandung was low. TB education program among DM patients needs to be improved.
Health-Seeking Behavior of Dengue Hemorrhagic Fever Patients in Several Hospitals in Bandung West Java Indonesia Afandi, Ressa Novita; Alisjahbana, Bachti; Raksanagara, Ardini S.
Althea Medical Journal Vol 5, No 3 (2018)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.378 KB) | DOI: 10.15850/amj.v5n3.1609

Abstract

Background: Dengue hemorrhagic fever is one of  the major global public health problems. Secondary prevention of dengue fever must be conducted to prevent high mortality rate by giving appropriate diagnosis and early management. This study was conducted to identify health-seeking behavior in dengue hemorrhagic patients and its related factors.Methods: This study involved 98 dengue hemorrhagic patients from four hospitals in Bandung region during July until November 2014. The hospitals were 2 public hospitals and 2 private hospitals. A questionnaire and in depth interview were used to collect the data.Results: Among 98 patients, 54 patients (55%) sought a right medical treatment timely, whereas 44 patients (45%) delayed. The first seeking behaviour to treat the disease was using over-the-counter drugs, followed by visiting a private clinic doctor. The second choice to seek health care was going to a private clinic doctor and hospital.Conclusions: Health-seeking behavior in dengue hemorrhagic patients in Bandung is inappropriate which can cause delay in treatment. 
EFEK PEMBERIAN SUPLEMEN SINBIOTIK DAN ZAT GIZI MIKRO (VITAMIN A DAN ZINC) TERHADAP STATUS GIZI PENDERITA TBC PARU ORANG DEWASA YANG MENGALAMI KEKURANGAN ENERGI KRONIK Hardinsyah, Suparman; Kusharto, Clara M; Sulaeman, Ahmad; Alisjahbana, Bachti
GIZI INDONESIA Vol 34, No 1 (2011): Maret 2011
Publisher : PERSATUAN AHLI GIZI INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.783 KB) | DOI: 10.36457/gizindo.v34i1.99

Abstract

Pasien  TB  paru  pada  akhirnya  akan  mengalami  keadaan gizi  buruk  dan  menurunnya  respon  imun. Kemoterapi  dengan  menggunakan  obat-obatan  TB  merupakan  langkah  yang  efektif  untuk  mengobati penyakit ini, tetapi mempunyai pengaruh negatif terhadap keseimbangan mikropola usus inflamasi karena infeksi TB paru, menurunkan pengaturan sintesa zat  gizi dan menurunkan nafsu makan, sehingga terjadi kekurangan  gizi.  Penelitian  bertujuan  untuk  menganalisa  efikasi  symbiotik  dan  suplemen zat  gizi  mikro terhadap status gizi pada pasien dewasa TB paru yang sedang diobati yang menderita kekurangan energi kronik  setelah  2  bulan  menjalani  intervensi.  Desain penelitian  ?a  double-blind  randomized  treatment control trial? dilakukan pada 2 pusat penyembuhan TB paru di Bandung dan Garut. Sejumlah 43 orang yang  menderita  kekurangan  energi  kronik  (KEK)  dipilih  dari  76  pasien  TB  paru  yang  terekrut  dengan variasi umur antara 20?45 tahun. Kelompok ini dibagi 2 kelompok, kelompok pertama diberikan susu yang berisi  protein,  symbiotik  dan  suplemen  micronutrien (MSM)  dan  kelompok  kedua  diberi  susu  yang berbasis protein saja (MO) sebagai kelompok kontrol. Seluruh pasien menerima terapi standar untuk TB paru, parameter status gizi (BB, IMT, masa Lemak, hemoglobin, serum vitamin A dan seng) dikumpulkan pada saat awal setelah 1 bulan, 2 bulan dari pemberian intervensi data di awalnya. Perbedaan antara dan dalam kelompok menggunakan statistik parametrik dannon parametric. Hasil menunjukan bahwa setiap kelompok  pada  akhir  intervensi  parameter  status  gizi  secara  signifikan  mengalami  perbaikan dibandingkan  pada  awal  penelitian  (P<0,05)  tetapi  tidak  ada  perbedaan  signifikan  antara  dua  kelompok (p>0,05). Ada pengaruh potensial dari setiap suplemen dalam meningkatkan status gizi, penelitian yang lebih lama dengan tidak memberikan susu berbasis protein pada kelompok kontrol dibutuhkan. Kata kunci: imunitas, zat gizi mikro, symbiotik, TBparu
Profil Ekspresi Koreseptor Human Immunodeficiency Virus CCR5 dan CXCR4 pada penderita Infeksi Human Immunodeficiency Virus yang menggunakan Narkoba Suntik Indrati, Agnes Rengga; Parwati, Ida; Garna, Herry; Alisjahbana, Bachti
Majalah Kedokteran Bandung Vol 50, No 3 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.559 KB) | DOI: 10.15395/mkb.v50n3.1230

Abstract

Infeksi human immunodeficiency virus masih merupakan masalah kesehatan global, termasuk di Indonesia dan pemakai narkoba suntik merupakan populasi yang besar di antara orang dengan  infeksi HIV/AIDS (ODHA) yang berperan penting dalam  transmisi infeksi HIV. Opioid banyak digunakan penasun dan memengaruhi ekspresi CCR5 serta CXCR4 yang merupakan  koreseptor yang digunakan oleh HIV untuk menginfeksi sel limfosit T CD4. Penelitian ini bertujuan menganalisisperbedaan  ekspresi koreseptor HIV CCR5 dan CXCR4 antara ODHA penasun dan ODHA bukan pemakai narkoba. Penelitian observasional analitik dengan  rancangan cross sectional ini dilaksanakan pada September 2011−Mei 2013. Subjek penelitian adalah ODHA penasun yang diikutsertakan secara consecutive sampling  di RSUP Dr. Hasan Sadikin Bandung  serta di komunitas di Jawa Barat. Pemeriksaan ekspresi koreseptor CCR5, CXCR4 dan jumlah sel limfosit T CD4 dilakukan dengan metode flowsitometri. Ditemukan 80 orang subjek penelitian dengan 17 orang pengguna narkoba suntik aktif, 16 orang dalam terapi metadon, 15 orang mantan penasun, dan 32 orang bukan pemakai narkoba. Persentase CXCR4 di permukaan sel limfosit T CD4 lebih tinggi bermakna pada ODHA bukan pemakai narkoba dibanding dengan ODHA penasun (86,13; 78,23; p 0,001). Tidak terdapat perbedaan bermakna  ekspresi CCR5 dan MFI CXCR4 di permukaan sel limfosit T CD4 antara ODHA penasun dan ODHA bukan pemakai narkoba (p>0,05).  Dari penelitian ini disimpulkan bahwaekspresi CXCR4  lebih tinggi pada ODHA penasun dibanding dengan ODHA bukan pemakai narkoba pada sel limfosit T CD4. Ekspresi koreseptor CXCR4 yang lebih tinggi dibanding dengan CCR5 baik pada kelompok ODHA penasun dan bukan pemakai narkoba.  Human Immunodeficiency Virus-Coreceptors CCR5 and CXCR4 Expression Profile in Injecting Drug Users Living with HIV  Human immunodeficiency virus (HIV) is considered as global health problem, includingin Indonesia. A large proportion of people living with HIV/AIDS (PLHAs) are Injecting Drug Users (IDUs) who play an important role in HIV transmission. Opioid that is used by many IDUs influence the expression of CCR5 and CXCR4, which are the co-receptors used by HIV to infect CD4 T lymphocyte cells. This study aimed to compare the expression of HV CCR5 and CXCR4 co-receptors between IDU PLHAs and non-drug user PLHAs. This was a crossectional analytical observation performed in the period of September 2011−May 2013 on IDU PLHAs who were sampled consecutively in Dr. Hasan Sadikin General Hospital Bandung and community in West Java. Flowcytometry was used for analyzing CCR5 and CXCR4 co-receptors as well as the CD4 T lymphocyte count. Eighty subjects were included in the study, consisting of 17 active injecting drug users, 16 IDUs on methadone therapy, 15 former IDUs, and 32 non-drug user PLHAs. The percentage of CXCR4 on CD4 T lymphocyte surface was significantly higher in non-drug user PLHAs when compared to IDU PLHAs (86,13; 78,23; p 0,001). No significant difference was found in the expression of CCR5 and MFI CXCR4 on CD4 T lymphocyte surface between IDU PLHAs and non-drug user PLHAs (p>0,05).  It is concluded that the expression of CXCR4 on CD4 T lymphocyte surface is higher in IDU PLHAs compared to non-drug user PLHAs. The expression of CXCR4 co-receptors is higher than the expression of CCR5 both in IDU PLHAs and non-drug user PLHAs.    
KESESUAIAN TERHADAP PROSEDUR STANDAR RESUSCITATION BUNDLE 6 JAM PADA PELAKSANAAN PENDERITA SEPSIS BERAT DAN SYOK SEPSIS Wirawan, Chevie; Alisjahbana, Bachti; Sumardi, Uun
Majalah Kedokteran Bandung Vol 50, No 1 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.169 KB) | DOI: 10.15395/mkb.v50n1.1157

Abstract

Sepsis berat dan syok sepsis memiliki angka kematian tinggi. Resuscitation bundle 6 jam adalah panduan tata laksana sepsis berat dan syok sepsis yang terbukti menurunkan mortalitas. Kesesuaian penatalaksanaan sepsis berat dan syok sepsis dalam 6 jam pertama dengan prosedur standar resuscitation bundle diteliti. Penelitian observasional deskriptif ini dilaksanakan selama September?Desember 2015 terhadap penderita sepsis berat dan syok sepsis di SMF Ilmu Penyakit Dalam RSUP Dr. Hasan Sadikin Bandung. Data dikumpulkan adalah data umum penderita dan pelaksanaan prosedur 6 jam pertama sesuai standar: pemeriksaan laktat, kultur darah, pemberian antibiotik, resusitasi cairan, pemberian vasopresor, pengukuran CVP, dan pemeriksaan Scvo2/Svo2. Dari 80 subjek penelitian, 59 (74%) sepsis berat dan 21 (26%) syok sepsis. Pada sepsis berat dan syok sepsis, dalam 3 jam pertama pemeriksaan laktat dilakukan pada 2%  dan 14%, kultur darah sebelum antibiotik 17% dan 10%, pemberian antibiotik spektrum luas 32% dan 43%, serta  resusitasi cairan 30 mL/kgBB 2% dan 14%. Pada syok sepsis dalam 6 jam pertama pemberian vasopresor 62% dan pengukuran CVP 5%. Pemeriksaan Scvo2/Svo2 tidak dilakukan pada syok sepsis. Angka kematian sepsis berat 7% dan syok sepsis 19,1% dalam 6 jam pertama. Simpulan, kesesuaian penatalaksanaan 6 jam pertama penderita sepsis berat dan syok sepsis dengan prosedur standar masih kurang.Kata kunci: Resuscitation bundle 6 jam, sepsis berat, syok sepsis  Compliance to 6-Hour Resuscitation Bundle Standard in Severe Sepsis and Septic Shock ManagementSevere sepsis and septic shock have a high mortality rate. Six-hour resuscitation bundle is a management standard for severe sepsis and septic shock that has been proven to reduce mortality rate. Compliance to the 6-hour resuscitation bundle standard was studied. This was a descriptive observational study on severe sepsis and septic shock patients in the Internal Medicine Department of Hasan Sadikin General Hospital Bandung in the period of September?December 2015. Data collected were patient?s general data and the implementation of the 6-hour management standard procedures that include lactate measurement, blood culture, broad spectrum antibiotics, fluid resuscitation, vasopressor use, CVP measurement, and Scvo2/Svo2 measurement. Of 80 subjects, 59 (74%) had severe sepsis and 21 (26%) had septic shock. In the first 3 hours, for severe sepsis and septic shock patients, lactate was examined in 2% and 14% respectively. For blood culture before antibiotics, broad spectrum antibiotic provision, and 30 mL/kgWt fluid resuscitation, the values were 17% and 10%, 32% and 43%; and 2% and 14%, respectively. In septic shock, in the 6 hours, vasopressor was given to 62% and CVP was measured in CVP 5%. Scvo2/Svo2 was not measured. The mortality rates in the first 6 hours for severe sepsis and septic shock were 7% and 19%. Compliance to first 6-hour standard procedure for severe sepsis and septic shock is still inadequate. Key words: Septic shock, severe sepsis, six hours resuscitation bundle
Gambaran Validitas Pemeriksaan Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Metode Rapid Immunochromatography pada Bahan Pemeriksaan Sputum dan Serum Penderita Tuberkulosis Paru Subroto, Hendra; Parwati, Ida; Turbawaty, Dewi Kartika; Alisjahbana, Bachti
Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.856 KB) | DOI: 10.15395/mkb.v49n3.1120

Abstract

Penegakan diagnosis tuberkulosis (TB) paru penting dalam mengurangi morbiditas dan mortalitas. Diagnosis laboratorium TB paru berdasar atas pemeriksaan BTA dan kultur M. tuberculosis memiliki sensitivitas rendah. Terdapat pemeriksaan cocktail antigen TB rapid immunochromatography (ICT) yang mendeteksi antigen ESAT-6, CFP-10, MPT-64 yang disekresikan oleh M. tuberculosis. Tujuan penelitian menganalisis validitas pemeriksaan cocktail antigen TB metode rapid ICT sputum dan serum penderita TB paru terhadap kultur Ogawa. Penelitian dilaksanakan Juli–Oktober 2014 di RSUP Dr. Hasan Sadikin Bandung. Bentuk penelitian adalah observasional deskriptif khusus dengan rancangan penelitian potong lintang. Subjek penelitian penderita yang datang ke Poliklinik Pulmonologi atau Poliklinik DOTS, didiagnosis TB paru. Sebanyak 68 sputum dan serum dari 33 kultur sputum M. tuberculosis positif dan 35 kultur negatif dilakukan pemeriksaan cocktail antigen TB rapid ICT. Angka positivitas cocktail antigen TB rapid ICT sputum 54,4%; serum tanpa pemanasan 0%. Pada serum dilakukan pemanasan pada suhu 56oC selama 30 menit untuk menghilangkan aktivitas antibodi dan didapatkan angka positivitas sebesar 19,1%. Nilai sensitivitas dan spesifisitas pemeriksaan untuk sputum 93,9% dan 82,8%, untuk serum tanpa pemanasan 0% dan 100%, serta serum dengan pemanasan 24,2% dan 85,7%.  Validitas pemeriksaan sputum memiliki sensitivitas tinggi dan spesifisitas sedang, untuk serum memiliki sensitivitas rendah dan spesifisitas tinggi. [MKB. 2017;49(3):178–85]Kata kunci: Cocktail antigen TB rapid ICT, kultur Ogawa, mikroskopik BTA, tuberkulosis paru, serum, sputum Validity of Complex Specific Cocktail Antigen Mycobacterium tuberculosis (ESAT-6, CFP-10, MPT-64) Rapid Immunochromatography Method on Sputum and Serum Samples from Patient with Pulmonary TuberculosisEarly diagnosis of pulmonary tuberculosis (TB) is very important in reducing morbidity and mortality. The current diagnosis of TB includes direct staining (acid fast bacilli) or M. tuberculosis culture, but these examinations have a low sensitivity. An assay using rapid ICT cocktail antigen TB is currently available for diagnosing TB. This method can detect ESAT-6, CFP-10, and MPT-64 antigen which is secreted by M. tuberculosis. The aim of this study was to analyze the validity of cocktail antigen TB rapid ICT using sputum and serum with Ogawa culture. This was a cross-sectional descriptive observational study. Sputum and serum were collected from patients who were diagnosed as lung TB suspects in the lung and DOTS Clinic of Dr. Hasan Sadikin General Hospital Bandung during the period of July–December 2014 in . Cocktail antigen TB detection assay using two kind of samples (sputum and serum) were evaluated. A total of 68 subjects of33 subjects presented positive culture and 35 presented negative cultures. Positivity rates for sputum and serum were 54.4% and 0%, respectively. Heated sputum assay had a sensitivity of 93.9% and specificity of 82.8%, Serum assay presented a sensitivity of 0% and specificity  of100%.  Serum were modified by heating at 56oC for 30 minutes. The positivity rate of heated serum was 19.1%. The result of modified serum assay showed a sensitivity  of 24.2% and specificity of 85.7%. Conclusion: the sensitivity of the sputum assay is high and the specificity is medium. The sensitivity of this serum assay is low and the specificity is high. [MKB. 2017;49(3):178–85]Key words: Acid fast bacilli, cocktail antigen TB rapid ICT, pulmonary tuberculosis, Ogawa culture, sputum, serum
Co-Authors Afandi, Ressa Novita Agnes Rengga Indrati Ahmad Sulaeman Amaylia Oehadian Andre van der Venn Angèle JGMGerver-Jansen Angky Tririni Anita Yuwita Anna Alisjahbana Ardini S. Raksanagara Ardini S. Raksanagara, Ardini S. Basti Andriyoko Budiman , Budiman Clara M Kusharto Dewi Kartika Turbawaty Dhiya Salsabila Dick van Soolingen Dida Ahmad Gurnida Dwi Agustian Edhyana Sahiratmadja Endang Sutedja Eva Mardiana Hidayat Evan Susandi Fauzian Giansyah Febriani, Ulfah Dwi Gumilang, Manik Intan Guntur Darmawan Hadyana Sukandar Hendra Subroto, Hendra Henriette A Delemarre–van de Waal Henry Chandra Herry Garna Hesti Lina Wiraswati Hidayat, Eva Mardiana Hikmat Permana Hinta Meijerink Hofiyah Djauhari Ida Parwati Ida Parwati Irvan Afriandi Irvan Afriandi Jeffery Malachi Candra Jose Batubara Juhariah Juhariah Julia Hartati Koesoemadinata, Raspati Cundarani Krishna Yana Krisnian, Tharani Kumia Wahyudi Kusumawardhani, R.N. Yasmin Lelly Yuniarti Leonardus Wiydatmoko marni banowati Menik Herdwiyanti Muhammad Yusuf Nanan Sekarwana Nanny Natalia Mulyani Soetedjo Ni Sayu Dewi B. Nisa Fauziah Novi Jayanti Nuni Sulastri Nurizzatun Nafsi Nurul Hidayah Chairunnisa Nury Fitria Dewi obin sarwita Panji Fortuna Hadisoemarto Parse, Rocci Jack Prayudi Santoso Putri Vidyaniati Raspati Cundarani Koesoemadinata Reinout van Crevel Reinout van Crevel Ressa Novita Afandi Resvi Livia Rocci Jack Parse Rovina Ruslami Rovina Ruslami, Rovina Santoso, Ida Parwati Sari Syahruni Siti Soidah Sofia Imaculata Suparman Hardinsyah, Suparman Susan Margaret McAllister Susantina Prodjosoewojo Syndi Nurmawati Teow Sheng Hao Tharani Krisnian Tjahjani M. Sudiro Tony Sadjimin Toto Subroto Trinugroho Heri Fadjari, Trinugroho Heri Tya Listiaty Ulfah Dwi Febriani Uun Sumardi, Uun Willem J Gerver Wirawan, Chevie Woro Indri Padmosiwi Yana, Krishna Yani Triyani Yani Triyani Yanti Mulyana Yeva Rosana Yovita Hartantri Yulniar Tasli