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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL GIZI INDONESIA Medical Journal of Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Makara Journal of Science Makara Journal of Technology
Nina Artanti
Research Center of Chemistry, Indonesian Institute of Scinces, Kawasan Puspiptek Serpong, Tangerang 15314, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology

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Journal : ANNALES BOGORIENSES

 1 
Pcr Amplification of Ornithine Decarboxylase (ODC) Gene Fragment from Tobacco (Nicotiana tabacum L.) cv. Temanggung Djajanegara, Ira; Pambudi, Sabar; Lestari, Retno; Artanti, Nina
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/11

Abstract

In order Lo create an antisense construct of the gene encoding Ornithine Decarboxylase (ODC) from tobacco (Nicotiana tabacum L.) cv. Temanggung, the target gene must be isolated. In this paper. we present the PCR amplification of a fragment from putative gene encoding ODC from tobacco cv. Temanggung. Leaf genomic DNA was i olated and used as the template for PCR. PCR optimization was done by adjusting the annealing temperature and the cycle number. Verification of the fragment obtained was also done using the second primer pairs .  
Isolation and Identification of Antiplasmodial Compound from Methanol Extract of Calophyllum bicolor P. F. Steven Abbas, Jamilah; Hanafi, Muhammad; Artanti, Nina; Sundowo, Andini; Minarti, Minarti; Asih, Puji Budi Setia; Syafrudin, Din
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i2.253

Abstract

Calophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Central Kalimantan. Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharmaceuticals. The aim of this research was to explore the antiplasmodial activity of methanol extract of Calophyllum bicolor P.F. Steven against Plasmodium falciparum. The methanol extract was purified by colomn chromatography system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1H-&13C-NMR and 2D-NMR, [COSY, HMBC and HMQC] data and the isolated compound was identified as xanthone. Methanol extract showed antiplasmodial activity growth inhibition against P. falciparum with IC50 5.2 ppm and the new 5-methoxy trapezifolixanthone compound have maximum inhibition at concentration 0.11 nMol.
Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Artanti, Nina; Maryani, Faiza; Mulyani, Hani; Dewi, Rizna Triana; Saraswati, Vienna; Murniasih, Tutik
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.451 KB) | DOI: 10.14203/ab.v20i1.234

Abstract

The marine bacteria were cultured in liquid medium under shaking condition were extracted with ethyl acetate. Antidiabetes was measured using inhibition of α-glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method.S creening results showed that at sample concentration of 200 µg/ml, there was significant α-glucosidase inhibitory activity was detected in the extracts of strain sp 7.9 (84% inhibition) and 8.10 (75% inhibition),however the antioxidant activity of these two strains were low only around 30% inhibition, antioxidant activities of other strains were very low.Screening for antibacterial activities using 10µl samples showed that extract of strain Sp 8.5was best for Staphylococcusaureus (14 mm inhibition); Sp 7.9 and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetes and antibacterial.Results of molecular identification conducted by INACC showed that identity of both strain based on BLAST Homology using NCBI database were Bacillus thuringiensis strain Ou2.
Isolation of cDNAs Encoding Asparagine Synthetase from Coronilla rostrata Artanti, Nina; Mcfarlane, Ian James
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.798 KB) | DOI: 10.1234/39

Abstract

Asparagine synthetase (AS) is one of the enzyme involves  in ammonium metabolism in plant, it catalyzes the transfer of the amino group of glutamine to aspartate giving asparagine. Asparagine serves as a major nitrogen transport and storage molecule in many higher plants. This research used legume plant Coronilla rostrata callus culture for primary and secondary metabolism study. On primary metabolism, the culture were used to study the phenomenon of asparagine accumulation. Because of the responsiveness of the culture, we  investigated the use of this culture for model system to conduct molecular biology study of the nitrogen metabolism. The aim of this study was to isolate...cDNAs enconding  Asparagine synthetase  from  C. rostrata because of the asparagine accumulate in this culture.  Several PCR based approaches  were conducted such as RT-PCR, LM-PCR and RACE. Using these methods, two AS cDNAs was  isolated from C. rostrata. AS1 (GenBank no AY081945) which a complete cDNA sequence and AS2 which a partial cDNA sequence.  (GenBank no AF488726). Thesetwo cDNAs had high homology with the legume AS. Keywords: cDNA, nitrogen metabolism, asparagine synthetase, Coronilla rostrata
Co-Authors A.A. Ketut Agung Cahyawan W Agustine Susilowati Akhmad Darmawan Andini Sundowo Aspiyanto Aspiyanto Dewijanti, Indah Dwiatmi Filiana Santoso Florence Ignatia, Florence Ignatia Galuh Widiyarti Gunawan Puteri, Maria Dewi Puspitasari Tirtaningtyas Hani Mulyani, Hani Heri Syahrian, Heri Ira Djajanegara Jamilah Abbas Ken Ichi Fujita, Ken Ichi Lia Meilawati Linar Zalinar Udin, Linar Zalinar Lotulung, Puspa Dewi N. Lotulung, Puspa Dewi Narij Maryani, Faiza Mcfarlane, Ian James Megawati - Minarti Minarti Pambudi, Sabar Phebe Hendra, Phebe Pratiwi, Rosa Dewi Puji Budi Setia Asih, Puji Budi Puspa D Lotulung, Puspa D Puspa Dewi Narrij Lotulung Puspa Lotulung, Puspa Rina Andriyani Rina Hidayati Pratiwi Rizna Dewi Rizna Triana Dewi Saraswati, Vienna Sofna D.S. Banjarnahor Supriadi, Edi Syafrudin, Din Teni Ernawati Tjiptadi, Felicia Melissa Tutik Murniasih Vienna Saraswaty, Vienna Yati Maryati Yoshinosuke Usuki, Yoshinosuke Zalinar Udin