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ISOLASI DAN SELEKSI RIZOBAKTERI YANG BERPOTENSI SEBAGAI AGEN PENGENDALI PANTOEA STEWARTII subsp. STEWARTII PENYEBAB LAYU STEWART PADA TANAMAN JAGUNG Rahma, Haliatur; Zainal, Aprizal; ., Suryati
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.975 KB) | DOI: 10.23960/j.hptt.216124-130

Abstract

Isolation and selection of rhizobacteria potentially as biocontrol agents against Pantoea stewartii subsp. stewartii causing stewart’s wilt disease in maize. Group of bacteria that colonize plant roots were known as Plant Growth Promoting Rhizobacteria (PGPR), in addition to capable of suppressing the development of pathogens also has the ability to improve plant health. This study aims to isolate rhizobacteria from rhizosphere of maize in West Sumatera. Rhizobacteria were characterized and tested for its ability to suppress the bacteria Pantoea stewartii subsp. stewartii causes stewart wilt’s disease on maize. In this research 15 isolates of rizobacteria potential as biological agent were found, i.e. 6 isolates of the fluorescens bacteria group, 5 isolates of non fluorescens bacteria group and 4 isolates of heat-resistant bacteria group.
POTENSI BAKTERI ENDOFIT DALAM MENEKAN PENYAKIT LAYU STEWART (PANTOEA STEWARTII SUBSP. STEWARTII) PADA TANAMAN JAGUNG Rahma, Haliatur; Zainal, Aprizal; Suharman, Memen; Sinaga, Meity S; ., Giyanto
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 14 No. 2 (2014): September, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.3 KB) | DOI: 10.23960/j.hptt.214121-127

Abstract

Potential of endophytic bacteria to control stewart wilt disease (Pantoea stewartii subsp. stewartii) in maize. The purpose of this study was to explore endophytic bacteria from seedling, maize roots and grass roots as well as to test the ability of endophytic bacteria which could potentially suppress stewart wilt disease development in maize. Characterization of endophytic bacteria as biocontrol agents including: do not induce HR on tobacco, synthesize IAA, dissolve phosphate, produce siderophores, and antibiotic to Pantoea stewartii subsp. stewartii (Pnss). The results of research shoed 17 isolates of endophytic bacteria potentially as candidate biocontrol agents. Nine isolates were able to produce IAA, siderofores and phosphatase; two isolates produce IAA and phosphatase; six isolates produce IAA. Six isolates ie: AR1, AJ34, AJ15, AJ19, and AJ14 AN6, can increase maize plant resistance and suppress stewart wilt disease severity with a range of 48.95-55.60%.
PENGEMBANGAN KAMPUNG DURIAN DI BATU BUSUAK, KELURAHAN LAMBUNG BUKIT, KOTA PADANG Hamid, Hasmiandy; Hayati, P.K. Dewi; Sutoyo, Sutoyo; Swasti, Etti; Zainal, Aprizal; Prasetyo, Teguh B Budi
LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 2 No 2(Jul-Des) (2018)
Publisher : Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Andalas Kampus Limau Manis - Padang 25163

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Daerah Batu Busuk, Kelurahan Lambung Bukit Kecamatan Pauh, Kotamadya Padang merupakan salah satu sentra produksi buah durian di kota Padang. Pada saat musim besar atau panen raya (in season) yang bisa berlangsung selama 1 bulan, produksi durian yang dihasilkan dari Batu Busuk diperkirakan mencapai 250–400 ton selama satu musim sedangkan pada musim panen “salek” atau panen kedua diperkirakan mencapai 150–250 ton.Walaupun nama durian Batu Busuk sudah terkenal dan banyak dicari oleh pencinta durian pada saat musim durian, namun demikian hingga kini belum ada upaya untuk mengangkat durian Batu Busuk sebagai buah identitas atau ikon kota Padang selain dari bengkuang. Juga belum ada upaya mengangkat daerah Batu Busuk sendiri sebagai kampung durian yang tidak hanya menghasilkan buah durian yang dijual keluar daerah, namun juga memiliki berbagai produk olahan hasil durian. Upaya yang dilakukan baru sejauh pemberian bantuan bibit durian oleh pemerintah daerah. Langkah awal untuk mengangkat nama durian Batu Busuk adalah membenahi identitas varietas durian Batu Busuk, karena sampai sekarang karakteristik dari durian Batu Busuk yang sesungguhnya tidak jelas. Tanaman yang sudah dikarakterisasi juga perlu diperbanyak agar kedepan, buah yang dihasilkan oleh kelurahan Batu Busuk adalah buah dengan karakter unggul tertentu. Masyarakat atau petani yang menanam durian juga perlu memiliki keahlian memperbanyak durian unggulnya. Penerapan ipteks yang akan dilakukan pada kegiatan pengabdian pada masyarakat ini menggunakan beberapa metode antara lain penyuluhan dan diskusi grup, demonstrasi dan pelatihan perbanyakan vegetatif melalui teknik sambung baik sambung pucuk (topgrafting)dan sambung matatunas(okulasi) serta demplot pembibitan durian. Kata Kunci: Karakteristik Durian Ungggul, Pendaftaran Varietas, Teknologi Tepat Guna, Teknik Sambung
Efektivitas Ekstrak Tumbuhan untuk Mengeliminasi Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Aprizal Zainal; Aswaldi Anwar; Satriyas Ilyas; , Sudarsono; , Giyanto
Indonesian Journal of Agronomy Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.645 KB) | DOI: 10.24831/jai.v38i1.1672

Abstract

Objectives of experiments were to evaluate (1) in vitro inhibitory effects of plant extracts on Clavibacter michiganensis subsp. michiganensis (Cmm), (2) inhibitory effects of plant extracts on Cmm infected tomato seeds, and (3) effectiveness of seed treatment plus plant extracts to eliminate Cmm. After evaluating 20 plant extracts, curcuma rhizome and betel vine leaf extract, cinnamon and clove oil were selected for further test. Tomato seeds were artificially inoculated with Cmm to obtain high level of infection. Part of the seeds were dipped in either suspension of selected extracts for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 °C and RH 60-70%) plus either of plant extracts oils, respectively. Elimination of Cmm level from infected seeds was observed at 10 days after treatments. Results of the experiment indicated curcuma extract, betel vine extract, cinnamon oil or clove oil showed in vitro inhibitory effects on Cmm. Moreover, dipping infected seeds in either 5% of curcuma, betel vine extract, or 0.5% of clove oil or matriconditioning plus these extracts oils were effective to eliminate Cmm from infected seeds. These treatments may potentially be used and developed commercially for eliminating seedborne Cmm on infected tomato seeds.   Keywords: Bacterial cancer, seedborne-pathogen, seed-treatments
Uji Inokulasi dan Respon Ketahanan 38 Genotipe To mat terhadap Clavibacter michiganensis subsp. michiganensis Aprizal Zainal; Aswaldi Anwar; Satriyas Ilyas; , Sudarsono; , Giyanto
Indonesian Journal of Agronomy Vol. 39 No. 2 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.368 KB) | DOI: 10.24831/jai.v39i2.4664

Abstract

Identifi kasi ketahanan genotipe adalah langkah awal dalam pengembangan kultivar tahan terhadap serangan patogen. Tujuan penelitian ini adalah (i) mendapatkan cara inokulasi dengan jumlah dan konsentrasi inokulum Cmm yang efektif untuk mengevaluasi ketahanan tomat terhadap Cmm di rumah kaca, (ii)  mendeterminasi reaksi ketahanan berbagai genotipe tomat akibat inokulasi Cmm. Percobaan ini menggunakan 38 genotipe tomat yang terdiri dari 7 genotipe tomat lokal, 15 genotipe tomat komersial, dan 16 genotipe koleksi Pusat Studi Pemuliaan Tanaman IPB Bogor (PSPT/IPB). Agen penyebab penyakit yang digunakan adalah 6 isolat Cmm hasil percobaan sebelumnya. Cara inokulasi Cmm yang efektif terhadap tomat cv. Marta (sangat rentan),  uji reaksi ketahanan berbagai genotipe tomat terhadap Cmm telah dilakukan di rumah kaca.  Kesimpulan dari penelitian ini adalah (i) inokulasi dengan menyuntikkan inokulum Cmm 5 µl konsentrasi 106 cfu/ml pada beberapa tempat di ketiak daun (daun pertama, daun tengah dan pucuk) merupakan cara yang paling efektif mengevaluasi ketahanan tomat terhadap Cmm, (ii) berbagai genotipe tomat yang diuji belum ada yang tahan teradap Cmm, genotipe tomat lokal ada yang agak rentan dan agak tahan.Kata kunci: kultivar tahan, suspensi bakteri, tomat
PENGELOMPOKAN TETUA PADI HIBRIDA BERDASARKAN SIFAT-SIFAT MORFOLOGI DAN RAPD-PCR Aprizal Zainal; Bahagiawati Amirhusin
Zuriat Vol 16, No 1 (2005)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v16i1.6778

Abstract

Penelitian ini bertujuan mengelompokkan tetua padi hibrida berdasarkan sifat-sifat morfologi dan Marka RAPD. Sepuluh RAPD primer polimorfis digunakan untuk menganalisis keragaman genetik 28 galur padi dengan analisis UPGMA. Analisis sifat-sifat morfologi didasarkan kepada 10 sifat kuantitatif dan dianalisis menurut metoda pautan rataan (Average Linkage). Analisis menggunakan marka RAPD pada tingkat kesamaan 76% didapatkan 5 kelompok tetua padi hibrida: I) IR58025A, IR58025B, IR62829A, IR62829B, IR68885A, C20, II) IR68886B, S4124F,  CISOKAN, IR65515-47-2-1-1-9, B10277-MR-1-4-3, B10373E-MR-1-3- 4-1-6, RHS 412-ICX-20X-OZH, IR30176-B-1-2-MR-2, IR53942, MAROS, IR68897B, IR68886A, DODOKAN, RCN-B-94-19, IR68, III) MTU 9992, B9154E-PN-1-1-4, IV) IR68885B, dan V) IR68888A, BR 827-35, IR68888B, IR68897A. Analisis berdasarkan sifat morfologi didapatkan 5 kelompok I) IR68897A, IR68888A, IR68885A, IR58025A, IR68886A, IR62829A, II) CISOKAN, B10277-MR-1-4-3, MTU 9992, IR58025B, DODOKAN, IR68897B, IR68888B, IR68886B, IR68885B, IR62829B, III) BR 827-35, IV) IR65515-47-2-1-1-9, C 20, MAROS, IR30176-B-1-2-MR-2, RCN-B-94-19, RHS 412-ICX-20X OZH, B9154E-PN-1-1-4, IR68, S4124F, IR53942, dan V) B10373E-MR-1-3-4-1-6. Dari penelitian terdahulu diketahui bahwa tetua padi hibrida yang didapatkan ber jarak genetik relatif jauh seperti IR58025A dan BR 827-35; IR58025A dan IR53942; IR58025A dan MAROS; serta IR58025A dan IR68 menghasilkan hybrid dengan hasil unggul. Penelitian ini menyatakan bahwa pengelompokan menurut morfologi tidak selalu sama dengan pengelompokan marka RAPD. Namun demikian beberapa galur CMS dan restorer sama-sama terdapat pada kelompok yang berbeda.
Distribution of Clavibacter michiganensis subsp. michiganensis in Various Tomato Production Centers in Sumatra and Java APRIZAL ZAINAL; ASWALDI ANWAR; UJANG KHAIRUL; . SUDARSONO
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.087 KB) | DOI: 10.5454/mi.2.2.3

Abstract

Bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis is a newly introduced disease of tomatoes in Indonesia. Its existence was first officially reported in 2004. The objective of this research was to monitor the existence of C. michiganensis subsp. michiganensis in various tomato production centers in Sumatra and Java. Tomato samples showing symptoms of C. michiganensis subsp. michiganensis infection were collected from various tomato production centers in Sumatera and Java and the causal agents were isolated from these samples. Based on the occurrences of typical symptoms of C. michiganensis subsp. michiganensis infection in tomato, the incidence of suspected C. michiganensis subsp. Michiganensis infection ranged from 1-20%. From a total of 74 tomato plants sampled, 24 bacterial isolates exhibiting similar colony morphology to C. michiganensis subsp. michiganensis were obtained. After various physiological, hypersensitive response, and pathogenicity tests, 18 isolates derived from 14 tomato production centers in eight provinces in Sumatera and Java were identified as C. michiganensis subsp. michiganensis. Although the incidence was still low, results of these experiments further indicated that C. michiganensis subsp. michiganensis existed in Indonesia and had spread in a number of tomato production centers in Sumatra and Java. Positive results of C. michiganensis subsp. Michiganensis identification from suspected tomato samples should be a warning sign for all tomato production stakeholders in Indonesia, especially those responsible in regulating the seed trade, importation, and plant quarantine
EFEKTIVITAS EKSTRAK TUMBUHAN MENGELIMINASI Clavibacter michiganensis Saubsp. Michiganensis DAN MEMPERTAHANKAN MUTU FISIOLOGIS BENIH TOMAT Aprizal Zainal; Aswaldi Anwar; Sudarsono .; Satriyas Ilyas; Giyanto .
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.841 KB) | DOI: 10.33512/j.agrtek.v3i1.567

Abstract

The objectives of the experiment were to evaluate effectiveness of seed treatments to eliminate Cmm of infected tomato seed lot, evaluate physiological quality of Cmm infected tomato seed lot after treatments with plant extracts. The research used curcuma rhizomes, betel vine leaf extract, cinnamon and clove oil to eliminate Cmm in tomato seed. Part of the seeds were dipped in either suspension of curcuma rhizomes, betel vine leaf extract, cinnamon and clove oil for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 0C and RH 60-70%) plus either of plant extracts or oils. Elimination of Cmm from infected seeds was observed 10 days after treatments, and physiological seed qualities were evaluated before and after seed treatments. Results of the experiment indicated, dipping infected seeds in either 5% of curcuma, betel vine extract, cinnamon oil or 0.5% of clove oil suspension or matriconditioning plus either these extracts, eliminated 99% of infected Cmm on tomato seeds. Seed treatments using plant extracts for elimination of Cmm did not reduce seed germination, germination rate, vigor index, and did not increase time to reach 50% total germination (T50) of infected tomato seed except the seed treatment by using 5% cinnamon oil with or without matriconditioning.Key words: plant extracts, physiological quality, seed viability, vigor
Pengembangan Durian Batu Busuak, Kelurahan Lambung Bukit, Kota Padang Hasmiandy Hamid; P.K. Dewi Hayati; Sutoyo Sutoyo; Etti Swasti; Aprizal Zainal; Teguh Budi Prasetyo; Panca Jarot Santoso
LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 2 No 2 (2018)
Publisher : Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Andalas Kampus Limau Manis - Padang, Sumatera Barat Indonesia-25163

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.134 KB) | DOI: 10.25077/logista.2.2.45-51.2018

Abstract

ABSTRAK: Kampung Batu Busuk Kelurahan Lambung Bukit Kecamatan Pauh merupakan salah satu sentra produksi buah durian di Sumatera Barat.Walaupun nama durian Batu Busuk sudah terkenal dan banyak dicari oleh pencinta durian pada saat musim durian, namun belum ada upaya untuk mengangkat durian Batu Busuk untuk bisa dikenal lebih luas. Langkah awal untuk mengangkat nama durian Batu Busuk adalah membenahi identitas varietas durian Batu Busuk, karena sampai sekarang karakteristik dari durian Batu Busuk yang sesungguhnya tidak jelas.Tanaman yang sudah dikarakterisasi juga perlu diperbanyak agar kedepan, buah yang dihasilkan oleh kelurahan Batu Busuk adalah buah dengan karakter unggul tertentu. Masyarakat atau petani yang menanam durian juga perlu memiliki keahlian memperbanyak durian unggulnya. Penerapan ipteks yang dilakukan pada kegiatan pengabdian pada masyarakat ini menggunakan beberapa metode antara lain penyuluhan, demonstrasi aplikasi dan demplot pembibitan durian. Hasil kegiatan menunjukkan bahwa kegiatan penyuluhan yang dilakukan berhasil meningkatkan pengetahuan masyarakat Batu Busuk mengenai arti penting pendaftaran varietas durian. Kegiatan demplot pembibitan durian dan demonstrasi teknik sambung meningkatkan pengetahuan dan keterampilan masyarakat mengenai propagasi durian. Kata kunci: Durian ungggul, Pendaftaran varietas, Teknologi tepat guna, Teknik sambung Development of Durian Batu Busuak, Lambung Bukit Village, Padang CityABSTRACT: Batu Busuk area, Lambung Bukit Village, Pauh Subdistrict, is one area of the centers of durian fruit production in West Sumatra. Although the Batu Busuk durian is already well-known and is sought after by durian lovers during the durian season, there is no attempt to lift the Batu Busuk durian to be known more widely. The first step to lifting the Batu Busuk durian is to fix the varieties identity of the Batu Busuk durian because until now the characteristics of the real Batu Busuk durian are not clear. Plants that have been characterized also need to be propagated so that in the future, the fruit produced by the Batu Busuk village is a fruit with certain superior characters. The community or farmers who plant durian also need to have the expertise to increase their superior durian. The application of science and technology conducted in community service activities uses several methods including counseling, demonstrations of applicationand demonstration plots of durian nurseries. The results of the activity showed the extension activities conducted succeeded in increasing the knowledge of the Batu Busuk community regarding the importance of registering durian varieties. The activities of the durian nursery demonstration plot and demonstration of connection techniques can increase the knowledge and skills of the community regarding the propagation of durian.Keywords: Superior durian, Varieties registration, Appropriate technology, Connection technique
PEMBERDAYAAN MASYARAKAT PALIMO INDAH KOTA PADANG MELALUI KEGIATAN PENGHIJAUAN LINGKUNGAN DI DAERAH PERKOTAAN Nugraha Ramadhan; Auzar Syarif; Roza Yunita; Fitri Ekawati; Winda Purnama Sari; Aprizal Zainal
Warta Pengabdian Andalas Vol 28 No 4 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.28.4.489-494.2021

Abstract

Reforestation is an effort to restore, maintain and improve the condition of the land so that it can be used optimally according to its original function. Several steps to realize the reforestation of the residential environment in a narrow area include utilizing the existing land, choosing suitable plants, and providing utmost care. This activity aimed to increase public understanding and knowledge regarding narrow land for reforestation in urban areas. The method used was a direct approach, namely, conducting interviews with the community and holding outreach and direct demonstrations in the field. The result of this empowerment activity was that the communities, especially the residents of Palimo Indah, are very enthusiastic about participating in the greening of the environment. The selected plants consider their multifunctionality for the environment's good, such as the absorption of air pollution and family medicinal plants. The three tree seedlings are useful for reforestation and have economic value, namely the Sungkai plant, Petai tree, and Agarwood sap-producing plant. Planting has been carried out at several location points by collaboration as a form of community empowerment, namely on the park's outskirts and on the riverbanks in the Palimo Indah, Pauh District, Padang.