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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro JSDH Indonesian Journal of Science and Technology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Menara Perkebunan Jurnal Jamu Indonesia EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Diah Ratnadewi
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Journal : JSDH

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Pertumbuhan Planlet Lidah Mertua (Sansevieria sp.) Blue Leaf dari Kultur Kalus Diah Ratnadewi; Ai Nurhasanah Husnul Izzati; Aris Tjahjoleksono
Jurnal Sumberdaya Hayati Vol. 1 No. 1 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.15-18

Abstract

Lidah mertua (Sansevieria sp.) Blue Leaf is a slow growing ornamental plant. It is also difficult to reproduce. Therefore market demands for this plant is impossible to fulfill through conventional methods of propagation such as leaf cutting or layering. Tissue culture is one of the alternatives to solve the problem of plant propagation. In vitro culture of Sansevieria calli was induced for shooting, and rooting afterwards. The basic media of WP was superior than MS in producing multiple shoots, and this WP media containing of BAP 5 mg/L and NAA 0,5 mg/L (WH1) gave the highest number of shoots and leaves on plantlets, as well as the best growth performance. The shoots started to appear two weeks after culture. Plantlets from WH1 rooted in higher frequency as well on rooting media. A mixture of compost, sand, and zeolit (4:4:1) enriched with a nutritive solution, composting of 1⁄4 WP plus IBA 0.5 mg/L, was a poreous media that was able to induce rooting of this plant.
Regenerasi Tanaman Kitolod (Hippobroma longiflora (L.) G.Don) pada Kultur In Vitro: Plant Regeneration of Kitolod (Hippobroma longiflora (L.) G.Don) in In Vitro Culture Fitri Nur`Aeni; Diah Ratnadewi; Sumaryono .
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.14-19

Abstract

Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant. Its flower is widely used as a traditional medicine. When this plant is utilized more intensively, there may be a shortage of the plant due to the lack of seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials. The experiments were arranged using a completely randomized design with two treatment factors and 10 replications for all experiments except in shoot rooting. Leaves and petioles were used as explant sources. Various combinations of benzilamino purine (BAP) and naphtalene acetic acid (NAA) were applied. Leaf explants in Murashige and Skoog (MS) medium enriched with 1 mg/L BAP and 0.1 mg/L NAA combination produced the highest number of adventitious shoots per explant, but 2 mg/L BAP + 0.1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots, and presented 75% of successful rate over the acclimatization period. The best rooting was provided by MS medium added with 0.5-1.0 mg/L NAA.
Co-Authors . Suharyo . SUMARYONO . TRIVADILA Adabiyah, Rifatul Ai Nurhasanah Husnul Izzati Anggraini Barlian ARIS TJAHJOLEKSONO Aryani Leksonowati Atik Choirul Hidajah Christofora Hanny Widjaja Darius Rupa Dian Rahma Pratiwi Dorly Dorly Dyah Iswantini Dyah Retno Wulandari Efah FITRAMALA Elsa May Susanti ENDANG HADIPOENTYANTI Erwin Al Hafiizh Eva KHAERUNNISA Fitri Nur`Aeni Hadi SUNARSO Hari Basuki Notobroto Hayati MINARSIH Ika Dewi Ana Imron RIYADI Khalisa Aini Sinaga Leksonowati, Aryani Lilis Solihat Maulidiyah Utami Muhamad Ayi Pandu Perdana Nina Ratna Djuita Nur Amallia Nurmuliayanti Muis Rahmi, Putri Rifatul Adabiyah Rinny Saputri Rinny Saputri Rizka Musdalifah Amsar Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirdjo Soekisman Tjitrosoedirjo Sumaryono . Titiek Setyawati Titiek Setyawati Titiek Setyawati Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Triati Dewi Kencana Wungu Wijayanta, Ardian Nur WIRDHATUL MUSLIHATIN Witjaksono Witjaksono Witjaksono Witjaksono WOLFGANG FRANK Yohana Caecilia Sulistyaningsih Yustiny Andaliza Hasibuan Zainal Alim Mas’ud Zainal Alim Mas’ud