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Journal : BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan)

EFFECTIVENESS TEST OF BLACK CUMIN SEEDS (NIGELLA SATIVA) EXTRACT ON THE GROWTH OF KLEBSIELLA PNEUMONIAE AND PSEUDOMONAS AERUGINOSA BACTERIA Nadya Nazimuddin Putri; Linda Chiuman; Chrismis Novalinda Ginting; Ermi Girsang
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 2 (2021): February 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i2.3702

Abstract

Black cumin seeds are known to have beneficial contents as antibacterial. This study aimed to determine the effectiveness of black cumin seed extract (Nigella sativa) on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa. The method used was the disc diffusion method. The ethanol extract of black cumin seeds gain by maceration method and was made into various concentration (5, 7.5, 10, 12.5, and 15)%. For positive control, ciprofloxacin disc were used and negative control used distilled water. The research model used was in vitro experimental research. The results showed that ethanol extract of black cumin seeds have antibacterial activity against Klebsiella pneumoniae with inhibition zone diameters (4.5, 6.7, 10.1, 11, 12.8) mm, Pseudomonas aeruginosa with inhibition zone diameters (7.5, 12, 12.6, 13.4, 15.6) mm, positive controls respectively (24.3 and 20.7) mm and negative controls (0). It can be concluded that black cumin seed extract has an inhibiting effect on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa.
EFFECTIVENESS TEST OF ANTI-BACTERIAL LIME JUICE (Citrus aurantifolia) ON SALMONELLA SP BACTERIA IN SALMON FISH (Oncorhynchus nerka) Qori Fadillah; Linda Chiuman; Chrismis Novalinda Ginting; Ermi Girsang
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.003 KB) | DOI: 10.31289/biolink.v7i1.3322

Abstract

Salmonella Sp is a gram-negative pathogens that are often found in marine foods,one of  process of serving  salmon is channeling lime juice, where lime juice is acidic and has phytochemical properties that have anti-bacterial properties. This study aims to explore anti-bacterial effect lime juice on Salmonella thypii bacteria in Salmon. This research is an experimental study using  disc diffusion method. Lime samples that were used were purchased from one of the modern markets in Medan which were made in several series of concentrations using distilled water as a solvent, while the salmon samples used in this study were purchased from one of the Japanese dining restaurants located in Medan City. Meanwhile, isolation of Salmonella thypii bacteria from salmon samples was carried out using nutrient broth and SSA media. The results of this study indicate that differences in concentration indicate the differences in the diameter of the inhibitory zone (P value = 0.000). With the diameter of the widest inhibition zone formed by a concentration of 100% is 15.40 ± 0.44 mm and the smallest diameter is formed by a concentration of 5% which is 7.23 ± 0.55 mm. Thus, it can be concluded that the lime juice has anti-bacterial activity against Salmonella thypii bacteria from salmon.