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Journal : Eksakta : Berkala Ilmiah Bidang MIPA

Pencil Lead Electrode Modified with Gold Thin Layer for Voltammetric Detection of Chromium (VI) Trisna Kumala Sari; Riga Riga; Moondra Zubir
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 2 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.742 KB) | DOI: 10.24036/eksakta/vol22-iss2/265

Abstract

Cr(VI) is a toxic, mutagenic, and carcinogenic metal. This heavy metal have effect harmful on organism and the environment. In this study, an electroanalytic approach was improved for detection of the Cr(VI) using a pencil lead electrode modified with gold thin layer by cyclic voltammetry. Gold thin layer was electrodeposited on the pencil lead electrode surface with potential-sweeping technique at scan of potential from 1.2 V to 0 V. Since the Cr(VI) species depends on the pH, effect of supporting electrolytes matrix at various pH were investigated. It was found that Cr(VI) gave a reduction peak with a peak potential of 0.35 V vs Ag/AgCl in cyclic voltammogram with 0.1M HClO4 as supporting electrolyte. The calibration curve for Cr(VI) at gold thin layer modified pencil lead electrode shows linearity in range of 5 µM to 100 µM with a detection limit of 2.3 µM achieved.
Study of The Antibacterial Activity of Endophytic Fungus That Colonize With The Twig of Andrographis paniculata Varel Anshar Al Khairi; Sri Benti Etika; Suryelita Suryelita; Mariam Ulfah; Riga Riga
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 2 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1063.338 KB) | DOI: 10.24036/eksakta/vol22-iss2/266

Abstract

Reports of the chemical constituents of Andrographis paniculata showed that this plant produced various secondary metabolites with antibacterial activity. Further exploration of bioactive compounds from A. paniculata can also be conducted by analyzing its endophytic fungi. Isolation of endophytic fungi from the twig of A. paniculata obtained three isolates of endophytic fungi. One of the isolates, RS-2, was fermented on rice media and extracted with ethyl acetate to give the EtOAc extract. The EtOAc extract from fungus RS-2 was analyzed for their antibacterial and phytochemical screening. The results exhibiting the EtOAc extract of fungus RS-2 has activity to inhibite bacterial growth. Overall, the study of the antibacterial activity of endophytic fungus obtained from the twig of A. paniculata was firstly carried out in this study