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Journal : Indonesian Journal of Chemical Science and Technology

Effectiveness Test of the Antibacterial Activity of Endophytic Fungi RS-2 from Sambiloto (Andrographis paniculata) on Red Rice Media Luqiyatur Rohmah; Suryelita Suryelita; Riga Riga; Mariam Ulfah
Indonesian Journal of Chemical Science and Technology (IJCST) Vol 6, No 1 (2023): JANUARY 2023
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/ijcst.v6i1.43181

Abstract

A. paniculata (Sambiloto) is a plant capable of producing various secondary metabolites which use in the field of pharmacology, one of which is as a source of antibacterial compounds. This potential can be explored from endophytic fungi associated with A. paniculata. The purpose was to determine the effectiveness of the antibacterial activity of the endophytic fungi RS-2 from branches of A. paniculata using red rice as the media. The stages of the research included isolation of the fungi, cultivation, extraction, antibacterial test, and test of the phytochemical of the endophytic fungi on branches of A. paniculata. The effectiveness test of antibacterial activity against the extract of the RS-2 endophytic fungi showed that the endophytic fungi extract had the potential to inhibit the growth of the three tested bacteria at concentrations of 10%, 30%, and 50%. The ethyl acetate extract of the endophytic fungi RS-2 from A. paniculata has the potential as a source of antibacterial compounds using red rice media.
Antibacterial Activity of Endophyte Fungus from Sambiloto Flowers (Andrographis paniculata) on Black Rice Growing Media Rizka Purwanti; Iryani Iryani; Riga Riga; Mariam Ulfah
Indonesian Journal of Chemical Science and Technology (IJCST) Vol 6, No 1 (2023): JANUARY 2023
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/ijcst.v6i1.43450

Abstract

Sambiloto plant (A. paniculata) is widely reported to have secondary metabolites that act as antibacterial. The potential is not only derived from the natural A. paniculata plant but can also be explored from its endophytic fungus. This study aimed to determine the antibacterial activity of ethyl acetate extract of endophytic fungus BS isolated from bitter flower (A. paniculata) on black rice growing media against Staphylococcus aureus, Streptococcus mutans, and Pseudomonas aeruginosa bacteria. The antibacterial activity test of the extract was carried out by disc diffusion method. The results obtained showed that the ethyl acetate extract of BS endophytic fungus grown on black rice media showed activity to inhibit the growth of Staphylococcus aureus, Streptococcus mutans, and Pseudomonas aeruginosa bacteria at concentrations of 10%, 30%, and 50%. In conclusion, the ethyl acetate extract of the endophytic mushroom BS isolated from the bitter flower (A. paniculata) can be used as an antibacterial.