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All Journal International Journal of Renewable Energy Development Jurnal Sains Materi Indonesia Indonesian Journal of Forestry Research Jurnal Penelitian Hasil Hutan Journal of Engineering and Technological Sciences Jurnal Riset Kimia Teknologi Indonesia Makara Journal of Technology
Widya FATRIASARI
R&D Unit for Biomaterials LIPI
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Energy Engineering Materials Science & Nanotechnology Mechanical Engineering

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Journal : Makara Journal of Technology

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Digestibility of Betung Bamboo Fiber Following Fungal Pretreatment Fatriasari, Widya; Syafii, Wasrin; Wistara, Nyoman J.; Syamsu, Khaswar; Prasetya, Bambang
Makara Journal of Technology Vol. 18, No. 2
Publisher : UI Scholars Hub

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Abstract

This research evaluated the effect of fungal pretreatment of betung bamboo fibers and enzymatic- and microwaveassisted hydrolysis on the reducing sugar yield. The enzymatic hydrolysis of the pretreated biomass was carried out with cellulase and 10 and 20 FPU/g of substrate in a shaking incubator at 50 °C and 150 rpm for 48 h. The sulfuric acid concentration used in the microwave-assisted acid hydrolysis was 1.0, 2.5, and 5%, either with or without the addition of activated carbon. Microwave irradiation (330 Watt) was applied for 5–12.5 min. The yield of reducing sugar was better with the microwave-assisted acid hydrolysis, and the yield tended to increase with an increase in the irradiation time. Based on the dry weight of the initial biomass (bamboo), pretreatment with 5% inoculum loading resulted in a higher reducing sugar yield (17.06%) than with 10% inoculum loading (14.54%). At a 1% acid concentration, the formation of brown compounds decreased, followed by a reduction in the reducing sugar yield. The addition of activated carbon at a 1% acid concentration seemed to be of no benefit with respect to the yield in the microwaveassisted acid hydrolysis. The pretreatment with the 5% inoculum loading for 12.5 min at 1% acid concentration resulted in the highest reducing sugar yield. Under these conditions, the yield was 6.3-fold that of the reducing sugar yield using 20 FPU/g of cellulase. The rate of bamboo hollocellulose hydrolysis reached 22.75% of the maximum theoretical reducing sugar reducing sugar of dry biomass.
Co-Authors Apri Heri Iswanto Ashrianis, Devi Nury Bambang Prasetya Budiman, Ismail Dede Heri Yuli YANTO Deni Zulfiana Euis HERMIATI Fahriya Puspita Sari Faizatul FALAH Fajriutami, Triyani Gutari, Sesmi hamzah, fajar nur I Nyoman Jaya Wistara Ina Winarni, Ina Ismayati, Maya Khaswar Syamsu Kurnia Wiji Prasetiyo Laksana, Raden Permana Budi Lubis, Muhammad Adly Rahandi Muhammad Ghozali Nanang Masruchin Nissa Nurfajrin Solihat Pratomo, Bagas Ikhasan Ramadhan, Kharisma Panji Ratih Damayanti Riksfardini Annisa ERMAWAR Sita Heris Anita Supriyanto Supriyanto Suryanegara, Lisman Wahyu Hidayat Wasrin Syafii dan Kurnia Sofyan Gustan Pari Buchari Yadav, Sumit Manohar