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Journal : Journal of Veterinary and Animal Sciences

Characteristics of Rumen Fluid, pH and Number of Microbia I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Journal of Veterinary and Animal Sciences Vol 4 No 1 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i01.p02

Abstract

Abstract. This research purpose is to determine the characteristics of pH rumen fluid and the amount of microbes in the rumen of Bali cattle. The research sample is healthy bali cattle rumen fluid which is slaughtered at Pesanggaran slaughterhouse Denpasar with a total of 30 samples. The research method uses conventional methods and refers to the method according to [6]. The total population of bacterial and fungal colonies was calculated by the pour plate method. Bacterial colonies were cultured on nutrient agar plates and fungi colonies were cultured on sabouraud dextrose agar plates. The Population of Enterobacteriaceae bacterial colonies was calculated by scatter method on Mac Conkey agar plate and Escherichia coli colony on Eosin Methylen Blue agar plate. Rumen liquid pH measurement is directly done with pH indicator strips. The study was conducted observationally and the data obtained were analyzed descriptively. The results showed that the total number of bacteria in the rumen fluid of Bali cattle with an average of 64x104 cfu/g, the number of fungal colonies with an average of 161x105 cfu/ g, the number of Coliform colonies was 23x104 cfu/g, the number of non-Coliform 28x103 cfu/g, E.coli 18x103 cfu/g and the mean The pH of Balinese cattle rumen fluid 6.9. Keywords : Balinese Cattle Rumen Fluid, Coliform, E. Coli , Fungi, non-Coliform, Total Bacteria , pH
Detection of Haemophilus Parasuis from The Respiratory Tract of Pigs I Gusti Ketut Suarjana; I Nengah Kerta Besung; Ketut Tono PG
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p03

Abstract

Study in order to detect the presence of Haemophilus parasuis in pig farms in Tabanan, Badung, and Gianyar Bali have been undertaken. A total of 197samples including nasal and tracheal swabs, organs and synovial fluids were collected from 69, 28 and 100 animals from pig farms in Tabanan, Badung and Gianyar regency, respectively. Samples were collected mainly from animals which showing clinical signs of coughing, asphyxiate, swollen joints and death animals. Isolation and identification of Haemophilus spp was performed following the methods described by Priadi et.al. (2004) and Nedbalkova et al. (2006) with slightly modification. Antimicrobial sensitivity test was performed using the disc diffusion method by Kirby Bauer.Haemophilus parasuis was detected from one sample which shown resistant to streptomycin only and sensitive to ampicillin, doxycycline and kanamycin, respectively.