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All Journal Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Advances in Tropical Biodiversity and Environmental Sciences Journal of Veterinary and Animal Sciences

Ketut Tono Pasek Gelgel

Laboratorium Mikrobiologi Veteriner Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 269006

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Uji Kepekaan Streptococcus spp. yang Diisolasi dari Penyakit Saluran Pernapasan Kompleks Babi terhadap Kanamycin, Streptomycin dan Doxycycline Dwi Arso Purba; Ketut Tono Pasek Gelgel; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Respiratory diseases in pigs often occur in the production of modern pigs around the world and known as Porcine Respiratory Disease Complex (PRDC). Prevention of PRDC can only be done if the infecting agent is bacteria. The bacteria that are most often isolated in complex pig duct disease are Streptococcus spp. This study was conducted to determine the sensitivity pattern of Streptococcus spp. isolated from animals with PRDC symptoms on kanamycin, streptomycin and doxycycline. Isolates were taken from the results of the PRDC case study of pig origin in Tabanan, Gianyar and Badung regencies. Pure isolates were then planted in blood agar and a sensitivity test was carried out using the Kirby-Bauer method for each antibiotic. Bacteria can be resistant, intermediate and sensitive to antibiotics tested. This gives rise to a pattern of sensitivity to antibiotics so that resistant germs will cause treatment failure. The results showed that hemolytic ? Streptococcus isolates were 60% sensitive to kanamycin, 100% sensitive to streptomycin, 40% resistant to kanamycin and 100% resistant to doxycycline. While hemolytic ? Streptococcus isolates were 100% sensitive to kanamycin and streptomycin but 100% were resistant to doxycycline.

Pengobatan Penyakit Pernapasan pada Babi dalam Upaya Meningkatkan Produktivitas Ternak di Desa Penarukan, Kerambitan, Tabanan I Gusti Ketut Suarjana; I Nengah Kerta Besung; Aida Lousie Tenden Rompis; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 12 No. 1 Pebruari 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Community service has been conducted on the treatment of respiratory diseases in pigs in an effort to increase livestock productivity in Desa Penarukan, Kerambitan, Tabanan. This dedication aims to provide knowledge to the community about how to manage pigs intensively, the prevention and eradication of diseases of pigs effectively and efficiently so that the maximum production of the livestock. Methods of service include house-to-house acts, especially to sickly breeders, initiating treatment with antibiotics, worm medicines, vitamins and minerals and providing counseling on how to prevent respiratory respiratory complex desease (PRDC) disease in pigs known as porcine respiratory complex desease (PRDC). The result obtained as follows: the community is give a positive response to activities and teams held home-to-home services to tens of breeders pigs whose reportedly suffered from PRDC spread over five banjar adat in Penarukan village. The sick pigs generally ranged from 1 to 3 months and treated as many as 20. Furthermore, based on the monitoring of the team and the report of breeders on the seventh day after being given treatment of the pigs showed no clinical symptoms and was declared cured.

Sensitivitas Isolat Escherichia coli Patogen dari Organ Ayam Pedaging Terinfeksi Koliseptikemia terhadap Oksitetrasiklin, Ampisilin dan Sulfametoksazol Yusmaniar Galuh Adi Luhung; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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This study aims to determine the sensitivity pattern pathogenic E. coli against antibiotics: oxytetracycline, ampicillin and sulfamethoxazole, in broilers in the Mangesta Village, Penebel, Tabanan Regency. The samples used in this research were 15 samples of colisepticaemia infected broiler’s organs . Sampels were isolated on media EMBA then identified with a Gram staining and biochemical tests with a TSIA, SIM, SCA and MRVP. Positive isolates of the bacterium E. coli were tested sensitivity to antibiotics oxytetracycline, ampicillin and sulfamethoxazole. The results showed that the sensitivity test of E. coli bacteria were isolated from the organs broilers suffered from colisepticaemia: 73.3% of E. coli resistant, 20% intermediate, and 6.7% was sensitive to the antibiotic oxytetracycline, 100% resistant to the antibiotic ampicillin, 53.3% and 46.7% intermediate resistance against sulphamethoxazole.

Total Bakteri Feses Ayam Petelur pada Berbagai Periode Pemeliharaan Dewa Putu Pradipta Baskara; I Nengah Kerta Besung; Ketut Tono PG
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims to determine the total number of bacteria isolated from laying hens at various ages. The sample used was healthy laying hens' feces with 24 samples using the pouring method. This study uses a completely randomized design (CRD). The data obtained were tested with the Least Significant Difference Test (LSD). The results showed the total number of bacteria in the starter phase of layer was 52.83x107 CFU/ml, grower phase of layer was 208.50x107 CFU/ml, developer phase of layer was 409.5x107 CFU/ml, and layer phases of layer was 208x107 CFU/ml. It can be concluded that the total number of bacteria in the layer is significantly different at each age phase.

Efek Toksisitas Ekstrak Daun Sirih Merah Terhadap Gambaran Mikroskopis Ginjal Tikus Putih Diabetik yang Diinduksi Aloksan Ni Made Rina Yulinta; Ketut Tono Pasek Gelgel; I Made Kardena
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.092 KB)

Penelitian ini bertujuan untuk mengetahui efek toksik dari ekstrak daun sirih merah terhadap gambaran mikroskopis ginjal tikus putih diabetes mellitus yang diinduksi aloksan. Sebanyak 20 ekor tikus putih jantan galur Sprague-dawley umur ± 3 bulan digunakan dalam penelitian ini. Seluruh sampel tersebut dibagi secara acak menjadi lima kelompok perlakuan yaitu: (P0) tikus sehat yang hanya diberikan aquades; (P1) tikus yang diberikan aloksan 120 mg/kg bb/intraperitoneal; (P2) tikus yang diberikan aloksan 120 mg/kg bb/intraperitoneal dan ekstrak daun sirih merah 50 mg/kg bb/peroral; (P3) tikus yang diberikan aloksan 120 mg/kg bb/intraperitoneal dan ekstrak daun sirih merah 100mg/kg bb/peroral; (P4) tikus yang diberikan aloksan 120mg/kg bb/intraperitoneal dan suspensi glibenklamid 1 mg/kgbb/peroral. Perlakuan diberikan selama 30 hari. Pada hari ke-31 semua tikus dieuthanasi dan dinekropsi untuk melihat gambaran mikroskopis ginjal. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sirih merah (Piper crocatum) dosis 50 mg/kg bb dan 100 mg/kg bb tidak menunjukkan perubahan patologi terhadap gambaran mikroskopis ginjal. Hal ini menunjukkan ekstrak daun sirih merah dosis 50 mg/kgbb dan dosis 100 mg/kgbb tidak toksik terhadap jaringan ginjal tikus putih diabetes mellitus.

Deteksi Bakteri Berpotensi Patogen Pada Penyakit Saluran Pernapasan Komplek Babi I Gusti Ketut Suarjana; I Nengah Kerta Besung; Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 14 No. 3 June 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims to detect bacterial infectious agents in pigs with complex respiratory tract disease known as porcine respiratory disease complex (PRDC) in some pig herds in Bali and look for the right medication is use to handle the incidence. The research sample comes from Tabanan, Badung and Gianyar totaling 48 Samples were isolated and identified by methods: isolation, the primary test, biochemical test. Test of sensitivity to antibiotics using disc diffusion method according to Kirby-Bauer method. The result showed that bacterial agents isolated from pigs were among others Streptococcus spp. alfa hemolytic non-coagulase (41,6%), Staphylococcus spp. beta hemolytic noncoagulase (41,6%) and Escherichia coli. Staphylococcus spp. beta hemolytic non-coagulase and E. coli were sensitive to streptomycin, kanamycin, doxicyclin, clindamycin and bacitracin. To conclude, Streptococcus spp. alfa hemolytic non-coagulase were sensitive to streptomycin, kanamycin, clindamycin and bacitracin, but resisten to doxicyclin.

Prevalensi Pasteurella multocida Pada Sapi Bali Di Bali I Nengah Kerta Besung; Ketut Tono PG; Aida Louis Tenden Rompis; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.772 KB)

This study aims to determine the prevalence of Pasteurella multocida in bali cattle in Bali. A total of 300 cows were taken from nasal swabs for isolation and identification of bacteria P.multocida. The samples came from Badung, Denpasar, Gianyar, and Bangli . Samples were grown on blood agar media. Separate colonies were identified by morphology, Gram stain, , Triple sugar Iron Agar, Methyl Red-Voger Proskauer (MRVP), Simmnons Citrate agar, Sulphid Indol Motility, sugars test and catalase test. Germs that characterizes P. multocida analyzed descriptively. The results showed that as many as 15 samples suffer from P. multocida bacteria spread in Badung 5 cattle (5%) , Denpasar 4 cattle (8%) , Gianyar 4 cattle (8%), and Bangli 2 cattle (2%).

Isolasi Klebsiella Sp. Pada Sapi Bali Berdasarkan Tingkat Kedewasaan Dan Lokasi pemeliharaan Serta Pola Kepekaan Terhadap Antibakteri Nyoman Anandiya Ramaditya; Ketut Tono PG; I Gusti Ketut Suarjana; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Research has been conducted to know the number bacterial isolates of Klebsiella sp. from bali cattle in terms of the level of maturity and the breeding location, also its sensitivity to the antibiotic there are ampicillin, sulfamethoxazole, and oxytetracycline. 120 bali cattle's nasal swabs consisting of calves, heifers, and adult cattle which are breeding on highland and lowland has been used as the isolates in this research. The obtained nasal swab was isolated and identified, then proceed with the sensitivity test. Bacterial of Klebsiella sp. has been found in calves were nine isolates (45%), heifers were six isolates (30%), and adult cattle were five isolates (25%). While based on the breeding location in the lowland found 13 isolates (65%) and highland found seven isolates (35%). In the sensitivity test results were obtained for the antibiotic ampicillin all isolates were sensitive (100%). sulfamethoxazole resistant four isolates (20%), intermediate two isolates (10%), and sensitive 14 isolates (70%). Oxytetracycline resistant seven isolates (35%), intermediate two isolates (10%), and sensitive 11 isolates (55%). The results showed that differences in the level of maturity and breeding location of bali cattle can affect the distribution of bacteria Klebsiella sp.

Efikasi Sterilisasi dan Desinfeksi Kandang untuk Mengurangi Infeksi Bakteri Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Vol. 12 No. 1 Pebruari 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Biosecurity efforts on chicken farms such as using antiseptics need to be modified so that the aim of suppressing the bacteria in the cage is effective. The purpose of this research is to find alternative methods for sterilization and disinfection of broiler cages and to find out the number and type of bacterial contamination in broiler cages. In this study two cages were used, the first cage washed with detergent and given Povidon Iodine disinfectant. In the second cage after washing with detergent, the floor of the cage is burned/heated and the cage wall is disinfected. Then the blood agar media is placed on the cage’s floor and opened for 1 hour. Then the blood agar media examined in the laboratory to see the number and type of bacteria that grow. All samples were tested with T-Test. The results showed that disinfection with disinfectants had more number of colonies (206 colonies, with an average of 29.85 colonies) than cages that used a heating method with a stove (182 colonies, with an average of 26 colonies). The results show there is no significant difference between sterilization treatment and disinfection treatment. The method of sterile sterilization by heating using a stove is slightly more effective than disinfection by using disinfectants seen from the number of bacterial colonies that grow, although the difference between the two methods is very little.

Escherichia coli pada Swab Rektum Ikan Koi yang Dipelihara di Kolam Isolasi pada Masa Karantina Kadek Apriyan Widiarta; Hapsari Mahatmi; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Koi fish (Cyprinus carpio) is one of the freshwater ornamental fish commodities which has a very high economic value and is of high interest. Koi fish are generally transported by land. This condition can cause koi to become stressed, which has an impact on decreasing health and even disease errors. One indicator of koi health problems can be seen from the presence of E. coli. This study aims to see the Escherichia coli bacteria in koi fish kept in isolation ponds during the quarantine period using 19 samples of isolates from rectal swabs which were then taken to BBVET Denpasar for isolation and identification of E. coli bacteria. This study used an exploratory observation design with a cross-sectional method, while the sample was taken by purposive sampling. The data obtained were descriptive qualitative. The result of this study are known from 19 samples that be examined show five samples positive of Escherichia coli bacteria (26,3%) which is three samples (15,9%) came from Tulungagung koi and two samples (10,3%) came from Blitar koi.

Co-Authors A.A. Ketut Agung Cahyawan W Ahmad Nuzuludin Kadri, Ahmad Nuzuludin Aida Lousie Tenden Rompis Anak Agung Gde Oka Dharmayuda Anak Agung Gede Agung Ananta Kusuma Anak Agung Sagung Kendran Astari, Ni Putu Widya Bolla, Nelci Elizabeth Daniel Opristanta Barus devinda yuniastika Dewa Putu Pradipta Baskara Dwi Arso Purba Fanayoni, Aditana Hanesty Jantiko, Hanesty Hapsari Mahatmi I BAGUS MADE BHASKARA I Gusti Ketut Suarjana I Kadek Juli Handriana I Ketut Berata I Ketut Suada I Made Adhi Kusuma Dwipayana I Made Kardena I Nengah Kerta Besung I Wayan Aris Suharsa Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Indra Manik Pradipta Kadek Apriyan Widiarta Ketut Budiasa Leny Beatry Veronica Luh Dewi Anggreni M Hasan Isnan Made Deddy Dharmana Putra Made Suma Anthara Matilda Krisnawati Nazara, Agustina Lesmauli Ni Ketut Suwiti Ni Made Rina Yulinta Ni Wayan Linda Pertiwi Nyoman Anandiya Ramaditya Pratanto, Aditya Putri Wiliantari Putu Devi Jayanti Putu Henrywaesa Sudipa Sabaaturohma, Clara Luceatriani Santoso, Sri Wahyuningsih Heri Trisno, Komang Voni Cornelia Br Sembiring Yusmaniar Galuh Adi Luhung

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