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All Journal Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Advances in Tropical Biodiversity and Environmental Sciences Journal of Veterinary and Animal Sciences

Ketut Tono Pasek Gelgel

Laboratorium Mikrobiologi Veteriner Fakultas Kedokteran Hewan Universitas Udayana

Author-ID : 269006

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Seroepidemiologi Septichaemia Epizootica Berdasarkan Jenis Kelamin pada Sapi Bali di Sumbawa I Nengah Kerta Besung; I Gusti Ketut Suarjana; Ketut Tono PG; Ni Ketut Suwiti
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (962.078 KB)

This study aims to determine the distribution map Septicaemiaepizootica (SE) by gender in Dompu, Bima and Sumbawa. A total of 240 serum samples derived from three districts in West Nusa Tenggara used the study sample. Serum was tested by ELISA (Enzyme Linked Immunosorbent Assay). Data samples positive and negative SE, presented descriptively. The results showed that seropositive SE at 18% bulls and cows by 12%. It can be concluded that the incidence of SE in the bulls higher than cows.

Isolasi dan Identifikasi Bakteri dari Susu Kambing Peranakan Etawa Terindikasi Mastitis Klinis di Beberapa Kecamatan di Kabupaten Banyuwangi M Hasan Isnan; Ketut Tono Pasek Gelgel; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.36 KB)

The purpose of this study was to determine the types of bacteria as a cause of clinical mastitis in goats Peranakan etawa in Banyuwangi. In this study, the sample used goat milk peranakan etawa in District Pesanggaran, Rogojampi, Songgon and Srono in Banyuwangi. As many as 11 samples were taken then analyzed in Laboratory Microbiology, Faculty of Veterinary Medicine Udayana University. Phase isolation and identification of samples were done to identify the bacteria that cause clinical mastitis through several stages of growth on Sheep Blood Agar (SBA), Gram stain, catalase test, test oxidase, growth in the TSIA and continued test Indol, Methyl Red, Voges Proskauer and citrate (IMViC ). Based on the results, namely mastitis-causing bacteria; Staphylococcus sp, Pseudomonas sp, Neisseria sp, E.coli, Corynebcaterium sp and Listeria sp. The conclusion of this study disease mastitis in goats Peranakan etawa in the District of Bayuwangi caused by Staphylococcus sp, Pseudomonas sp, Neisseria sp, E.coli, Corynebcaterium sp and Listeria sp.

Pola Kepekaan E coli Yang Diisolasi Dari Feses Broiler Penderita Diare Terhadap Sulfametoksazol, Ampisilin Dan Oksitetrasiklin (SENSITIVITY PATTERN OF E. coli ISOLATED FROM PATIENTS WITH DIARRHEA BROILER FAECES AGAINST SULFAMETOKSAZOLE, AMPICILLIN I Wayan Aris Suharsa; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 7 No. 2 Agustus 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.872 KB)

Broiler merupakan jenis ayam yang paling cepat berproduksi dan dagingnya memiliki mutu yang tinggi dibandingkan dengan jenis ayam ras lainnya. Penelitian ini bertujuan untuk mengetahuikepekaan bakteri Escherichia coli (E. coli) sebagai penyebab kolibasilosis terhadapsulfametoksazol, ampisilin, dan oksitetrasiklin pada peternakan broiler di Banjar Tangkub, DesaPayangan. Bakteri E. coli diisolasi dari 30 sampel feses broiler penderita diare diuji kepekaanyadengan menggunakan metode Kirby-Bauer dan dianalisis secara deskriptif. Hasil penelitianmenunjukkan bahwa sebanyak 25 isolat resisten (83,33%), satu isolat intermediet dan sisanyaempat isolat sensitif terhadap antibiotika oksitetrasiklin. Sedangkan terhadap antibiotika ampisilin,sejumlah 24 isolat (86,67%) menunjukkan resisten dan sisanya yakni empat isolat sensitif terhadapantibiotika tersebut. Terhadap sulfametoksazol, kuman E coli yang diisolasi menunjukkan 29 isolatresisten (96,67%) dan satu isolat sensitif. Berdasarkan hasil penelitian dapat disimpulkan bahwapenggunaan sulfametoksazol, ampisilin dan oksitetrasiklin sebaiknya dihindari.

Tinjauan Ekonomi Persentase Karkas Sapi Bali Berdasarkan Asal Ternak yang Dipotong di Rumah Pemotongan Hewan Mambal, Badung Bali devinda yuniastika; Ketut Tono Pasek Gelgel; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Bali cattle are known to have a high percentage of carcasses. This study aims to determine the percentage of bali cattle carcasses from highland and lowland areas that were slaughtered in RPH Mambal Abiansemal district, Badung regency, Bali province, and to find out the selling price of carcasses from both regions. This study used 56 bali cattle from Bangli and Gianyar regencies. Data on the percentage of carcass based on animal origin were analyzed using Independent Samples T-Test to see whether there were differences. The relationship between life weight and carcass weight of Bali cattle and the total selling price is used by power regression analysis (Sampurna, 2012). The results showed a higher percentage of the carcass in Bangli Regency, resulting in higher and more profitable selling.

Bakteri Coliform dan Non Coliform yang Diisolasi dari Saluran Pernapasan Sapi Bali Putri Wiliantari; I Nengah Kerta Besung; Ketut Tono PG
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

The main purpose of this study is to determine the coliform and non coliform bacteria at the respiratory tract of Bali cattle that raised at the lowlands and highlands. 36 Samples were taken on calves, heifers, and adults, then grown on Salmonella Shigella Agar medium by spreading method. Coliform colonies and non coliform colonies were analyzed variably and followed by Least Significant Difference (LSD) test. The results showed that the number of coliform bacteria of bali cattle at the highlands was 1287.96 CFU/g and at the lowlands was 643.9798 CFU/g and non coliform bacteria at the highlands was 162.2464 CFU/g and at the lowlands as much as 81.12321 CFU/ g. Coliform and non coliform bacteria were obtained at the highlands and the lowlands showed no significant differences.

Pola Kepekaan E. coli Yang Diisolasi Dari Feses Burung Kicau Penderita Diare Terhadap Antibiotik Sulfametoksazol, Ampisilin, Dan Oksitetrasiklin (SENSITIVITY PATTERN OF E. coli ISOLATED FROM BIRD CHIRPING WITH DIARRHEA FECES AGAINST ANTIBIOTICS) I Kadek Juli Handriana; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 7 No. 2 Agustus 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.98 KB)

This study aims were to determine the sensitivity pattern of Escherichia coli bacteria isolatedfrom the feces of birds chirping with diarrhea against antibiotic sulfamethoxazole, ampicillin, andoksitetrasiklin in Satria and Sanglah bird market.The results showed that all 30 isolates from birdfaeces were examined, sulfamethoxazole sensitive 3.3%, intermediate 13.3%, and resistant 83.3%.In oxytetracycline 3.3% sensitive, 40% intermediate, and 56.7% resistant . While sensitive to ampicillin 13.3%, 23.3% intermediate, and 63.3% resistant.

Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Ayam Petelur I Nengah Kerta Besung; I Gusti Ketut Suarjana; I Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

The study aim was to determine the resistance of Escherichia coli to antibiotics that isolated in laying hens. A total of 100 samples of chicken feces were taken from Tabanan, Bangli and Karangasem districts. E. coli were isolated and identified by Carter (1990) method, followed by a bacterial susceptibility test with agar diffusion method from Kirby Bauer against antibiotics namely oxytetracycline (30 µg), ampicillin (10 µg), streptomycin (10 µg), sulfamethoxazole (30 µg), chloramphenicol (30 µg), and kanamycin (30 µg). The obtained data compared to the next sensitivity standard of the bacteria that classified as sensitive, intermediate, and resistant. The results showed that E. coli from chicken feces are sensitive to kanamycin, whereas the other antibiotics are intermediate or resistant. The increase in resistance is in line to the age of the chicken.

Isolasi dan Identifikasi Bakteri Shigella spp. Penyebab Diare pada Anjing Voni Cornelia Br Sembiring; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p08

Dogs are one of the favorite animals that are kept by many people because they are considered intelligent and loyal animals. One of the main health problems of dogs is diarrhea caused by a bacterial infection. Shigella spp. is a pathogenic bacterium in the digestive tract of dogs. This study aims to isolate and identify Shigella spp. bacteria that cause diarrhea in dogs. Samples studied using rectal swabs in dogs showing clinical symptoms of diarrhea with a total of 48 samples from veterinary clinics in the city of Denpasar and its surroundings. Isolation of Shigella spp. was conducted on Eosin Methylene Blue Agar, Salmonella-Shigella Agar, and Sheep Blood Agar. Colonies that grew and separated on Salmonella-Shigella Agar medium were subjected to Gram staining, catalase test, oxidase test and continued with biochemical tests, namely Triple Sugar Iron Agar, Sulfid Indole Motility, Methyl Red Voges Proskauer, Simmons Citrate Agar, and test for sugars such as glucose, lactose, mannitol, and arabinose. The results showed that of the 48 isolated and identified samples, one sample showed a positive result for Shigella spp. with possibly the species Shigella dysentriae. It was concluded that one (2.08%) of Shigella spp. bacteria could be isolated and identified from 48 samples of dog rectal swabs that showed clinical symptoms of diarrhea. It is necessary to conduct further research on the level of pathogenicity of Shigella spp. bacteria and for further research, it is necessary to supplement the API 20E test to determine the Shigella species.

Isolasi dan Identifikasi Enterobacter spp. pada Anjing Diare Matilda Krisnawati; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p07

This study aims to isolate and identify Enterobacter spp. from the digestive tract of dogs with diarrhea. Diarrhea is a digestive disease that commonly attacks dogs, one of which can be caused by Enterobacter spp. This study using 48 samples in the form of a dog with diarrhea rectal swabs from Denpasar and surrounding areas. The samples were isolated on Eosin Methylene Blue Agar, MacConkey, and Blood Agar media by streak method and then incubated for 24 hours at 37°C, then primary tests were carried out, namely gram staining, catalase test, and oxidase test, and identification tests were carried out on Triple media. Sugar Iron Agar, Simon Citrate Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, and sugar test. The results showed that two (4.16%) positive samples of Enterobacter spp. diarrhea in dogs. Therefore it can be concluded that Enterobacter spp. can be isolated and identified in the digestive tract of dogs with diarrhea. Howewer, it is necessary to carry out identification test for each Enterobacter species and more in-depth research on the pathogenicity of Enterobacter spp.

Uji Sensitivitas Bakteri Klebsiella spp. yang Diisolasi dari Anjing Kintamani Diare Terhadap Antibiotika Anak Agung Gede Agung Ananta Kusuma; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p07

This study aims to determine the pattern of sensitivity of bacteria Klebsiella spp. isolated from kintamani dogs with diarrhea. The sample of this study were 4 isolates of Klebsiella spp. isolated from kintamani dogs with diarrhea. The method used in this study is the Kirby-Bauer disc diffusion method in duplicate using the antibiotics Kanamycin, Sulfamethoxazole-trimethoprim, and Streptomycin. The data obtained were expressed descriptively qualitatively by measuring the antibiotic inhibition zone formed. The results showed that isolates of Klebsiella spp. 75% intermediate (3 isolates) and 25%(1 isolate) sensitive to Kanamycin, all samples sensitive to Sulfamethoxazole-trimethoprim and 75% (3 isolates) sample intermediate and 25% (1 isolate) sensitive to Streptomycin.

Co-Authors A.A. Ketut Agung Cahyawan W Ahmad Nuzuludin Kadri, Ahmad Nuzuludin Aida Lousie Tenden Rompis Anak Agung Gde Oka Dharmayuda Anak Agung Gede Agung Ananta Kusuma Anak Agung Sagung Kendran Astari, Ni Putu Widya Bolla, Nelci Elizabeth Daniel Opristanta Barus devinda yuniastika Dewa Putu Pradipta Baskara Dwi Arso Purba Fanayoni, Aditana Hanesty Jantiko, Hanesty Hapsari Mahatmi I BAGUS MADE BHASKARA I Gusti Ketut Suarjana I Kadek Juli Handriana I Ketut Berata I Ketut Suada I Made Adhi Kusuma Dwipayana I Made Kardena I Nengah Kerta Besung I Wayan Aris Suharsa Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Indra Manik Pradipta Kadek Apriyan Widiarta Ketut Budiasa Leny Beatry Veronica Luh Dewi Anggreni M Hasan Isnan Made Deddy Dharmana Putra Made Suma Anthara Matilda Krisnawati Nazara, Agustina Lesmauli Ni Ketut Suwiti Ni Made Rina Yulinta Ni Wayan Linda Pertiwi Nyoman Anandiya Ramaditya Pratanto, Aditya Putri Wiliantari Putu Devi Jayanti Putu Henrywaesa Sudipa Sabaaturohma, Clara Luceatriani Santoso, Sri Wahyuningsih Heri Trisno, Komang Voni Cornelia Br Sembiring Yusmaniar Galuh Adi Luhung

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