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All Journal Jurnal Sain Veteriner Jurnal Veteriner Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Hastari Wuryastuti
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Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Keterkaitan Panhisterektomi dan Suplemen 1,25- Dihidroksivitamin D3 dengan Risiko Urolitiasis pada Tikus (CORRELATION BETWEEN PANHISTERCTOMY AND 1.25-DIHYDROXYVITAMIN D3 SUPPLEMENTATION ON RATS UROLITHIASIS RISK) Hartiningsih .; Devita Anggraeni; Irkham Widiyono; Hastari Wuryastuti
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this research was to study the correlation of panhisterectomy and supplement 1.25-dihydroxyvitamin D3 on urolithiasis risk in Wistar rats. Twenty female Wistar rats at 8 weeks of age, weredivided into four groups (control fed standard diet, control fed standard diet+1,25-dihydroxyvitamin D3 supplement, panhisterectomy fed standard diet and panhisterectomy fed standard diet +1,25-dihydroxyvitamin D3 supplement). Eleven weeks after treatment, each of rats was placed into individualmetabolic cage for balance study for a week. From day 4 to 11 of the balance study, every morning theremaining food, feces, and urine were collected and recorded for calcium (Ca) analysis. At the end ofbalance study, blood samples were taken from canthus retroorbitalis medialis for estrogen analysis. Theresults showed urinary and fecal Ca excretions were not significantly different compared to the controlgroup. Calcium consumption was significantly higher (P<0.05) in panhisterectomized rats compared withthose in control rats. While, estrogen in panhisterectomized group was not significantly different to thosein control rats. Calcium urinary and Ca consumption in rats consuming 1,25-dihydroxyvitamin D3 supplement were significantly higher (P<0.05) compared with those in without 1,25-dihydroxyvitamin D3 supplementation, but Ca excretion in feses was not significantly different. Estrogen in rats consuming1.25-dihydroxyvitamin D3 supplement was significantly lower (P<0.05) compared with the rats that without1,25-dihydroxyvitamin D3 supplemention. It can be concluded that panhisterectomy does not seem to affecturolithiasis risk, while 1,25-dihydroxyvitamin D3 supplement may affect urolithiasis risk. There is likelyno association between panhisterectomy and 1.25-dihydroxyvitamin D3 supplementation on urolithiasisrisk in Wistar rats.
Gambaran Histopatologi Insang Ikan Mas di Daerah Endemik Koi Herpesvirus (HISTOPATHOGIC FINDINGS OF GILLS OF THE COMMON CARPS IN THE ENDEMIC AREA OF KOI HERPESVIRUS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Koi herpesvirus (KHV)  is the cause of  a lethal disease that affects common carp (Cyprinus carpio)  andkoi (Cyprinus carpio koi). Although, it has been reported that common carps could act as carriers for KHV,their histopathologic findings, especially the gills  have not been identified up to now. In the present study,12  normal, healthy looking common carps including their gills were collected from the endemic area ofKHV in  Sleman, Yogyakarta. All fish were necropsied and the gills were collected and fixed in 10% bufferformalin. Then, the gills were processed histopathologically using routine hematoxyline-eosin stain andexamined under the microscope. Histopathologic examination of the gills exhibited an apparent infiltrationof inflammatory cells, especially lymphocytes. The large oval to polygonal basophilic cells containing largeintranuclear inclusion bodies were also identified. Gills epithelial cells show mass hyperplasia and adhesionwith necrotic changes. Thus, results of this study has led to a reasonable conclusion that KHV infection ispresent in the normal, healthy common carps. One possibility is those KHV  are live viruses (carriers forKHV) and might could act as a source of infection is being discussed.
Identifikasi Koi Herpesvirus dengan Uji Imunopatologi Imunohistokimia Streptavidin Biotin pada Ikan Mas Karier (IDENTIFICATION OF KOI HERPESVIRUS USING IMMUNOPATHOLOGIC IMMUNOHISTOCHEMISTRY OF STREPTAVIDIN BIOTIN IN THE COMMON CARP CARRIERS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In  managing the koi herpesvirus (KHV) outbreaks as a routine national program in Indonesia, testingbased on biotechnology, such as  immunopathologic immunohistochemical approach(es) using antibodythat is safe, rapid  and accurate need to be applied. This will hopely assist the Government of Indonesianin improving and enhancing the sustainability of national animal proteins program. The present studywas aimed to develop and apply the immunopathologic immunohistochemistry of streptavidin biotin (IHCSB) for detection of KHV in the apparently normal carps. The gills from 48 common carps  (Cyprinuscarpio) that appear to be healthy were prepared for  DNA-based KHV  by IHC SB.  Common carps werecollected from fish farms which had an outbreak of KHV in 2008-2009 in Yogyakarta.  All fish werenecropsied. The gills were processed histopathologically and then stained for IHC SB with monoclonalantibody anti-KHV. We demonstrated that all of the fish gills were positive for KHV antigen. Thus, it isconcluded that method is useful and consistent, very sensitive and rapid, and is a reliable method to beapplied for field condition to detect antigen KHV in the gills of normal, healthy looking carps.   In addition,and more importantly, the fish can act as a source of KHV (carriers for KHV) and may result in the spreadof diseases among susceptible fish.
Deteksi Virus Avian influenza Pada Ayam Pedaging Komersial yang di Suplementasi Water Additive Rina Isnawati; I Putu Cahyadi Putra; Rina Dwi Susiani; Hastari Wuryastuti; R. Wasito
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Penyakit Avian influenza (AI) merupakan penyakit infeksius pada unggas yang disebabkan oleh avian influenza virus (AIV) telah mewabah dan menyebabkan kerugian ekonomi yang cukup besar pada industri perunggasan di Indonesia serta bersifat zoonosis. Penurunan kejadian AI pada manusia perlu difokuskan pada pencegahan dan kontrol infeksi pada unggas. Tujuan penelitian ini adalah deteksi dini dengan pendekatan molekuler dan imunologis untuk mencegah penyebaran AIV dengan menjaga kesehatan ayam pedaging komersial yang di suplementasi water additive. Lima puluh lima ekor DOC ayam pedaging strain Cobb digunakan sebagai hewan coba selama 35 hari. Lima ekor day old chick (DOC) dipilih secara acak dan dinekropsi untuk diambil organ paru-paru. Lima puluh ekor ayam yang tersisa di bagi menjadi dua kelompok perlakuan yaitu kelompok kontrol (K) dan perlakuan (P). Setiap minggunya (minggu ke-1 sampai ke-5) sebanyak lima ekor ayam pada masing-masing kelompok di pilih secara acak untuk dinekropsi diambil organ paru-paru. Deteksi AIV dilakukan dengan Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) terhadap gen matrix (MA) dan hemagglutinin (H5, H7, H9), sedangkan deteksi imunologis imunohistokimia streptavidin biotin (IHK-SB) diamati dengan mikroskop. Hasil penelitian ini adalah sampel paru-paru (P) minggu pertama dan kedua terdeteksi MA, namun tidak terdeteksi H5, H7, H9. Hasil IHK-SB pada kelompok K positif VAI, sedangkan pada kelompok P positif VAI pada minggu pertama dan kedua, minggu ketiga negatif. Kesimpulan dari penelitian ini adalah terdapat infeksi alami VAI tipe A, Water additive yang tersedia secara komersial dapat mencegah infeksi alami VAI pada minggu ketiga setelah pemberian.
Co-Authors Asmarani Kusumawati Bambang Sutrisno Devita Anggraeni Guntari Titik Mulyani Hartiningsih . Hartiningsih . Hary Purnamaningsih I Putu Cahyadi Putra Irkham Widiyono J.Sri Widada Joannes Sri Widada Leksono Lestariyadi Mohammad Amien Nazaruddini . Prapti K. R. Wasito R. Wasito . Raden Wasito Rina Dwi Susiani Rina Isnawati Sapto Yuliani Siti Chalimah Soedarmanto Indarjulianto Sri Andayani Sri Hartati Sumartono . Wasito . Wasito Wasito Yuni Indraswati