Garuda - Garba Rujukan Digital

Seroprevalens serta faktor-faktor risiko toksoplasmosis pada penduduk di Desa Kubu Kabupaten Karangasem Bali Laksmi, Dewa Ayu; Sudarmaja, I Made; Swastika, I Kadek; Damayanti, Putu Ayu Asri; Diarthini, Ni Luh Putu Eka
Medicina Vol 47 No 1 (2016): Januari 2016
Publisher : Medicina

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.586 KB)

Toksoplasmosis menjadi salah satu masalah kesehatan masyarakat di seluruh dunia karena dapat menyebabkan kelainan kongenital pada neonatus. Tujuan dari penelitian ini adalah untuk menentukan prevalens dan mengidentifikasi faktor-faktor risiko toksoplasmosis pada penduduk di Desa Kubu Karangasem. Sampel serum dikumpulkan dari penduduk di beberapa banjar secara consecutive. Kuesioner diberikan untuk memperoleh data tentang demografi dan faktor risiko toksoplasmosis. Faktor risiko yang diteliti adalah kontak dengan tanah, kebiasaan pola makan termasuk konsumsi daging mentah atau setengah matang, sayuran yang tidak dicuci, dan sumber air minum. Besar sampel dalam penelitian ini adalah 106 orang dengan usia rerata 45,20 (SB 17,03) tahun. Prevalens IgG anti-Toksoplasma gondii di Kabupaten Kubu adalah 13,2%. Seroprevalens tertinggi toksoplasmosis dalam penelitian ini adalah 20%, ditemukan pada usia 50-59 tahun. Prevalens kelompok usia reproduksi (20-49 tahun) pada populasi penelitian adalah 15%. Faktor risiko yang signifikan terkait dengan toksoplasmosis adalah sumber air minum. Disimpulkan bahwa seroprevalens toksoplasmosis pada penduduk di Desa Kubu Kabupaten Karangasem Bali sebesar 13,3%, dan sumber air minum merupakan faktor risiko yang terkait. Toxoplasmosis has become one of public health problem worldwide because it can cause congenital malformation in newborn. The objective of this study was to determine the seroprevalence and to identify risk factors of toxoplasmosis in population of Kubu district Karangasem. We collected serum samples consecutively from resident of several banjar in Kubu. A questionnaire was administered to elicit data on demographics and practices considered to be risk factors for toxoplasmosis. Risk factor that had been evaluated in this study were contact with soil, food pattern habits including consumption of rare/ improperly cooked meat and unwashed vegetables, drinking water sources. The total sample in this study were 106 people with an average age of 45.20 (SD 17,03) years. Seroprevalence of IgG anti- Toksoplasma gondii in Kubu district was 13.2%. The highest seroprevalence of toxoplasmosis in this study was 20%, found in the age 50-59 years. The prevalence of reproductive age group (20-49 years ) in the study population was 15%. Risk factors significant associated with toxoplasmosis was source of drinking water. We conclude that seroprevalence of toxoplasmosis in population of Kubu district Karangasem was 13.2%, and source of drinking water was a significant risk factor.

PERBANDINGAN PENDEKATAN BELAJAR (LEARNING APPROACHES) MAHASISWA TAHUN PERTAMA DAN TAHUN KETIGA FAKULTAS KEDOKTERAN UNIVERSITAS UDAYANA Made Indira Dianti Sanjiwani; Ni Putu Wardani; Ni Luh Putu Eka Diarthini; Dewa Ayu Agus Sri Laksemi
E-Jurnal Medika Udayana Vol 10 No 10 (2021): Vol 10 No 10(2021): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2021.V10.i10.P13

Learning approach is a method applied by individuals in studying material to achieve learning outcomes. Learning approaches can be classified into surface, deep, and strategic learning approach. Changes in the learning environment may affect the learning process of a student. This is prone to occur in first-year medical students who are experiencing a transition from high school education to university. The purpose of this study was to explore the comparison of the learning approaches used by first and third-year medical students. This study is an analytical study with a cross-sectional approach using the Indonesian version of The Approaches and Study Skills Inventory for Students (ASSIST) questionnaire. The sample collection technique used random sampling method with a sample size of 216 students. The collected data were analyzed in univariate and bivariate ways using a computer program. Respondents ranging from 18 to 22 years old and most of them were females (66.2%). First-year student respondents were 106 people (49.1%) and third-year students were 110 people (50.9%). Comparative analysis for categorical data was carried out using the chi-square test and obtained p-value = 0.011 (p <0.05), indicates that there is a significant difference between the types of learning approaches of first and third-year medical students. Third-year students were found to have a deep learning approach dominantly, while first-year students have a surface learning approach dominantly. Further research related to learning approaches should be carried out as a contribution to the curriculum formation process and may encourage students to adopt a good learning approach

HUBUNGAN PERILAKU HIGIENITAS DIRI DAN SANITASI SEKOLAH DENGAN INFEKSI SOIL TRANSMITTED HELMINTHS PADA SISWA KELAS III-VI SEKOLAH DASAR NEGERI NO. 5 DELOD PEKEN TABANAN TAHUN 2014 Ni Luh Gede Dian Ratna Dewi; Dewa Ayu Agus Sri Laksmi
E-Jurnal Medika Udayana Vol 6 No 5 (2017): E-jurnal medika udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.752 KB)

Infeksi kecacingan yang disebabkan oleh soil transmitted helminths (STH) merupakan masalah kesehatan masyarakat Indonesia. Infeksi soil transmitted helminths sangat sering ditemui di Asia Tenggara termasuk Indonesia. Letak geografis Indonesia yang beriklim tropis sesuai untuk perkembangan parasit. Tujuan dari dilakukannya penelitian ini adalah untuk mengetahui hubungan antara perilaku higienitas diri dan sanitasi sekolah dengan kejadian infeksi soil transmitted helminths di Sekolah Dasar No. 5 Delod Peken Tabanan. Penelitian ini merupakan studi analitik observasional dengan pendekatan cross-sectional untuk menilai hubungan penyakit kecacingan akibat infeksi soil transmitted helminths (STH) dengan perilaku higienis dan sanitasi sekolah. Dari 105 sampel, didapatkan prevalensi infeksi kecacingan STH sebesar 7,6%. Berdasarkan jenis cacing, infeksi terbanyak terjadi karena Trichuris trichiura (55,6%), Hookworm (22,2%), Enterobius vermicularis (11,1%), dan Ascaris lumbricoides (11,1%). Sebagian besar anak tergolong dengan higenitas baik (65,7%), sementara 34,3% tergolong dengan higenitas buruk. Terdapat hubungan yang signifikan antara higenitas diri dengan infeksi STH (P=0,012), dimana faktor yang mempengaruhi adalah aktifitas mencuci tangan (P=0,001) dan kontak dengan tanah (P=0,003). Sementara tidak didapatkan hubungan infeksi STH dengan jenis kelamin (P=1), penggunaan alas kaki (P=0,333), dan kebersihan kuku (P=0,141).

Mosquito-specific viruses (family Flaviviridae, genus Flavivirus) Diisolasi pada Nyamuk Anopheles vagus di Bali Putu Ayu Asri Damayanti; I Nyoman Mantik Astawa; Anak Agung Ayu Mirah Adi; I Made Sudarmaja; I Kadek Swastika; Dewa Ayu Agus Sri Laksemi; Ni Luh Putu Eka Diarthini
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Mosquito-specific viruses (MSVs) adalah virus yang hanya dapat bereplikasi pada sel nyamuk. Virus ini terdiri dari berbagai genus, salah satunya yang paling banyak ditemukan adalah dari famili Flaviviridae, genus Flavivirus. Namun, data keberadaan dan karakteristik MSVs dan vektornya di Bali saat ini sangat terbatas. Oleh karena itu, pengamatan untuk memperluas penemuan keragaman vektor dan filogenetik MSVs famili Flaviviridae, genus Flavivirus di Bali dilakukan pada tahun 2016-2018. Nyamuk dewasa ditangkap menggunakan light trap dan dikelompokkan berdasarkan spesies. Isolasi dan propagasi virus dilakukan pada galur sel C6/36 dan baby hamster kidney-21 (BHK-21). Identifikasi virus dilakukan dengan menggunakan one step reverse-transcriptase polymerase chain reaction (RT-PCR). Terdapat dua pool yang berasal dari nyamuk Anopheles vagus menampakan cythopathic effect (CPE) hanya pada galur sel C6/36 dari total 158 pool. Virus yang diisolasi memiliki persentase identity sekuen nukleotida tertinggi 97% dan sekuen asam amino 96% dengan virus Culex theileri Flavivirus isolat JKT-8650 yang diisolasi pada tahun 1981. Selanjutnya, virus dinamakan Mosquito Flavivirus Isolate Bali (MFB) dengan accession numbers KY995166 dan KY290258. Analisis filogenetik menunjukan bahwa MFB berada satu kluster dengan Culex theileri Flavivirus (CTFV) dari Indonesia, Culex Flavivuruses-Myanmar, Culex theileri Flavivirus-Portugal, dan Mosquito Flavivirus-Turki. Terdapat delapan nukelotida dan enam asam amino yang berbeda antara MFB dan CTFV Indonesia. Pada penelitian ini dapat disimpulkan bahwa MSVs dari famili Flaviviridae, genus Flavivirus berhasil diisolasi dari nyamuk An. vagus di Bali.

Seroprevalensi yang Tinggi dan Faktor-Faktor Risiko Toksoplasmosis pada Darah Donor dan Wanita di Bali (HIGH SEROPREVALENCE AND RISK FACTORS OF TOKSOPLASMOSIS AMONG BLOOD DONORS AND WOMEN IN BALI) Dewa Ayu Agus Sri Laksemi; Wayan Tunas Artama; Mahardika Agus Wijayanti
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.775 KB)

Toxoplasmosis is an important public health problem because of its worldwide distribution, economicand social impact due to high sequele that may cause such as mental retardation and blindness in children.The aims of this study were to asses serological prevalence of toxoplasmosis in donors and women in Baliand get an overview of association between risk factors and toxoplasmosis infection, i.e.: comprising catownership, food pattern, occupation related to contact with raw meat and activities related to contact withsoil. Serum samples were collected from donors consecutively, while simple cluster design was used forsampling woman. Data on demographics and risk factors for toxoplasmosis were obtained usingquestionnaire. Serological prevalence of toxoplasmosis in donors was 35,9%, while in women was 63.9%.Serological prevalence of toxoplasmosis in donors at District Badung was 29,2%, Tabanan 36.8%, Gianyar25.0%, Denpasar 41.1%, Klungkung 25.0%, and Bangli 8.3%. Serological prevalence of toxoplasmosis inwomen at District Badung was 33.3%, Tabanan 66.5%, Gianyar 82.5%, Denpasar 71.1%, Klungkung 81.5%and Bangli 16.7%. Risk factor that play a role in toxoplasmosis infection were food pattern and occupationrelated to contact with soil. The seroprevalence of toxoplasmosis in voluntary blood donors and child-bearing age is relatively high due to local habbit of Balinese society that consume raw meat called lawarand sate

Penurunan Kadar Trigliserida pada Tikus Jantan dengan Terapi Growth Hormone (DECREASE OF TRIGLYCERIDE LEVEL IN MALE RAT BY GROWTH HORMONE TREATMENT) I Gusti Ayu Dewi Ratnayanti; I Wayan Sugiritama; Ida Ayu Ika Wahyuniari; Ni Made Linawati; I Gusti Ngurah Mayun; Dewa Ayu Agus Sri Laksemi; I Gusti Ngurah Sri Wiryawan; I Gusti Kamasan Nyoman Arijana
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.93 KB)

The use of growth hormone (GH) as cardiovascular disease treatment is still controversial. In thispreliminary study the effect of growth hormone therapy on plasma triglyceride level in dyslipidemia wasexamined. Pre and post control group design study was done using 20 dyslipidemic (total cholesterol >200mg/dL) male rats, age 11–12 month-old. The subjects were divided into four groups, aquadest (P0), GH0.02 IU/day (P1), GH 0.04 IU/day (P2), and GH 0.08 IU/day (P3). All subjects were given high cholesteroldiet for three weeks to achieve dyslipidemic in blood level. Aquadest and GH were injected subcutaneouslyonce daily for two weeks. Triglyceride plasma level was measured on day 22nd and 38th by using colorimetricenzymatic test. The mean of pre test plasma triglyceride level of all groups was 136.30 mg/dL and nosignificant difference was found among the groups (p > 0.05). Growth hormone therapy significantly reducedplasma triglyceride level of P1 by 11.78% (118.82 mg/dL, p < 0.01), P2 by 23.46% (103.41 mg/dL, p < 0.01),and P3 by 35.15% (90.22 mg/dL, p < 0.01). Comparison of post test data amomg the groups showedsignificant difference (p < 0.01). This study show that growth hormone therapy could reduce plasmatriglyceride level in dyslipidemic rat. However, further research is needed to more understand the effect ofthe therapy on cardiovascular diseases.

Risk factors of soil transmitted helminth infection among primary school students I Gede Gita Sastrawan; Jordaniel Setiabudi; Ni Putu Sanjiwani; Ni Komang Indriyani; Dewa Ayu Laksemi
Health Science Journal of Indonesia Vol 11 No 2 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i2.2885

Latar belakang: Infeksi kronis dari soil transmitted helminth (STH) dapat menyebabkan gangguan gizi, pertumbuhan dan kognitif pada anak. Untuk mengurangi dampak infeksi STH, diperlukan identifikasi faktor risiko. Penelitian ini bertujuan untuk mengidentifikasi faktor risiko yang berhubungan dengan infeksi STH pada siswa sekolah dasar di Desa Seraya Timur, Karangasem, Bali. Metode: Penelitian ini menggunakan desain potong lintang dengan mengambil total sampel. Penelitian dilaksanakan pada bulan Januari 2020. Data primer mengenai faktor-faktor risiko infeksi STH dikumpulkan dengan menggunakan kuesioner. Diagnosis infeksi STH dilakukan dengan pemeriksaan tinja dengan metode Kato-Katz. Analisis data menggunakan uji chi-square untuk menentukan faktor risiko yang berhubungan dengan infeksi STH. Hasil: Sebanyak 83 siswa yang berusia 6-12 tahun berpartisipasi dalam penelitian ini. Terdapat 9 siswa (10.84%) yang terinfeksi STH dengan intensitas infeksi ringan. Sebanyak 55.56% terinfeksi Trichuris trichiura, 33.33% terinfeksi Ascaris lumbricoides dan 11.11% terinfeksi cacing tambang. Terdapat beberapa faktor risiko yang memiliki hubungan bermakna dengan infeksi STH diantaranya adalah sering bermain tanah (OR=6.86; 95%CI 1.326-35.494), bermain tanpa alas kaki (OR=10.5; 95%CI 1.249-88.278) tidak mencuci tangan setelah bermain tanah (OR=9.450; 95%CI 1.809-49.358) dan tidak memotong kuku secara rutin (OR=6.462; 95%CI 1.250-33.388). Pemberian obat cacing setiap enam bulan mampu memberikan efek proteksi terhadap infeksi STH (OR=0.085; 95%CI 0.016-0.449). Kesimpulan: Kebersihan diri menjadi faktor risiko yang berhubungan dengan infeksi STH. Direkomendasikan untuk meningkatkan promosi kesehatan terkait kebersihan diri disamping pemberian obat cacing setiap enam bulan. Kata kunci: Faktor risiko, infeksi STH, anak sekolah dasar Abstract Background: Chronic soil transmitted helminth (STH) infection might cause nutritional, growth and cognitive impairment in children. Identifying the risk factors of STH infection is crucially needed to minimize the infection effects. This study aimed to identify risk factors associated with STH infections among primary school students in Seraya Timur Village, Karangasem, Bali. Methods: This study used a cross-sectional design with a total sampling method. The study was conducted in January 2020. Risk factors data were collected using a questionnaire. The diagnosis of STH infection was done by stool examination with the Kato-Katz method. The chi-square test was used to determine the risk factors associated with STH infection. Results: 83 students with ages ranging from 6-12 years participated in this study. There were 9 students (10.84%) whose infected with mild infection of STH. 55.56% of students were infected by Trichuris trichiura, 33.33% were Ascaris lumbricoides infections and 11.11% were hookworm infections. There were several risk factors that significantly associated with STH infection including ground’s playing (OR=6.86; 95%CI 1.326-35.494), barefoot (OR=10.5; 95%CI 1.249-88.278), did not wash hands after playing soil (OR=9.450; 95%CI 1.809-49.358) and did not routinely cut their nails (OR=6.462; 95%CI 1.250-33.388). Deworming every six months could provide a protective effect against STH infection (OR=0.085; 95%CI 0.016-0.449). Conclusion: Personal hygiene is a risk factor associated with STH infection. It is recommended to increase personal hygiene promotion besides dewormed every six months. Keywords: Risk factors, STH infection, primary school students

Ethanol Extract of Spondias pinnata Leaves Reduce Parasite Number and Increase Macrophage Phagocytosis Capacity of Mice Infected by Plasmodium berghei Dewa Ayu Agus Sri Laksemi; I Gusti Kamasan Arijana; I Made Sudarmaja; Ni Luh Ariwati; Ketut Tunas; Putu Ayu Asri Damayanti; Ni Luh Putu Eka Diarthini; I Kadek Swastika; Ida Ayu Dewi Wiryantini
The Indonesian Biomedical Journal Vol 13, No 1 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i1.1286

BACKGROUND: Currently, there is no vaccine against malaria in humans, the development of resistance to anti-malarial drugs, causing the need to find new alternatives to overcome malaria infections. This study aimed to determine effect of Spondias pinnata in increasing cellular immunity, especially phagocytosis activity of peritoneal macrophages against Plasmodium berghei infection.METHODS: This was an experimental study with two stages of research, each stage requires 36 Balb/c mice, aged 2 months and weight 20-25 grams. After one week of acclimatization, the mice were put into 6 different groups, each group consisted of 6 mice. The negative control was a group of mice given distilled water for 14 days then infected by P. berghei in the 15th day. Meanwhile, T1, T2, T3, T4 and T5 groups were given S. pinnata leaves ethanol extract with dose of 25, 50, 100, 200 and 400 mg/kg body weight (BW)/day, respectively, and then infected by P. berghei in the 15th day.RESULTS: The results showed that the lowest parasitemia and the highest capacity of macrophage to phagocytose latex was found in treatment group T3 that received 50 mg/kg BW of S. pinnata leaves ethanol extract. Based on analysis of the Pearson correlation test, there was a significant correlation between percent phagocytosis and parasitemia (p<0.05).CONCLUSION: Ethanol extract of S. pinnata leaves lower the parasite number of P. berghei in Balb/c mice and increase the capacity of macrophage to phagocytose latex. However, the mechanisms of how S. pinnata leaves extract in activating phagocytosis capacity and reducing parasitemia still need further investigation.KEYWORDS: phagocytosis, Plasmodium berghei, parasite number, Spondias pinnata

UJI AKTIVITAS BUAH MAHKOTA DEWA (PHALERIA MACROCARPA) SEBAGAI ANTIMALARIA PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI I Kadek Ade Juniantara; Dewa Ayu Agus Sri Laksemi; Putu Ayu Asri Damayanti; Ni Luh Putu Eka Diarthini
E-Jurnal Medika Udayana Vol 11 No 12 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Malaria is a parasitic infection caused by Plasmodium sp. Malaria’s medication and prevention are known, but there is a problem of increasing Plasmodium resistance to antimalarial drugs such as drugs from the chloroquine class, to overcome this problem traditional medicine is chosen because it is safer and cheaper. God’s crown (Phaleria macrocarpa) is one of the plants commonly used for traditional medicine which contains several active substances, namely saponins, polyphenols, flavonoids, tannins, sterols, alkaloids and terpenoids which have potential as antimalarials which can be proven by observing the degree of parasitemia in the mice blood. This study was conducted in vivo using the Randomized Post Test Only Controlled Group method using 24 samples of mice which were divided into 4 groups. The negative control group was only 0.2 ml of RPMI as a treatment control and the treatment group was treated with God’s crown extract at doses of 1, 10, and 100 mg/kgBW, then the degree of parasitemia was examined in each mouse. The data analysis technique used is One Way ANOVA and Post Hoc test. The results obtained mean the degree of parasitemia in the control group, doses of 1 mg/kgBW, 10 mg/kgBW and 100 mg/kgBW respectively 41.70; 37.96; 31.55; and 37.0. The results based on the analysis using One Way ANOVA posttest, it was found that there were significant differences between the control group and treatment groups 1,2, and 3 with p value of 0.000 (p < 0.05). The results showed that the extract of the fruit of the God’s crown (Phaleria macrocarpa) could inhibit the growth of Plasmodium berghei, the best dose was found at a dose of 10 mg/kgBW.

Title

Found 9 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search