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HISTOPATOLOGI HATI MENCIT PASCA PEMBERIAN SUSPENSI KEPEL (Stelechocarpus burahol) SECARA INTRAGASTRIK SELAMA 14 HARI The Histopathology of Mice Liver Treated by Kepel (Stelechocarpus burahol) Suspension Intragastrically for 14 days Harlina, Eva; Sa’diah, Siti; S Darusman, Huda; Alvernita, Gita
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 2, No 1 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

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This aim of this study was to examine the effect of Kepel (Stelechocarpus burahol) to the mice hepatocytes. Thirty male mice of 4 week aged were divided into three groups; control group was treated by aquadest, Dose 1x group was treated by 2.6 mg/g BW/day kepel powder (0.5 ml kepel suspension/day), and Dose 5x group was treated by 13 mg/g BW/day kepel powder (1.0 ml kepel suspension/day). The treatment was intragastrically for 14 days. The mice were euthanized and necropsy followed by the liver collection for histopathology assay. The histopathological examination of liver showed hydropic degeneration, cell death and extramedullary hematopoietic observed on mice hepatocytes. The ANOVA analysis showed that kepel caused increase significantly (p<0.05) of hydropic degeneration and decrease significantly (p<0.05) of cell death of mice hepatocytes.Key words: Stelechocarpus burahol, hydropic degeneration, cell death, extramedullary hematopoietic

Aktivitas Diuretik dan Analisa Mineral Urin Perlakuan Ekstrak Tanaman Kumis Kucing (Orthosiphon Stamineus Benth) pada Tikus Jantan Madyastuti, Rini; Ietje wientarsih; Setyo Widodo; Erni H Purwaningsih; Eva Harlina
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Tanaman Kumis Kucing merupakan salah satu tanaman yang memiliki banyak keuntungan dan sudah digunakan sejak dahulu dalam upaya menjaga kesehatan. Tujuan dari penelitian ini adalah mengevaluasi aktivitas diuretik dari penggunaan ekstrak Kumis Kucing dan mengukur kadar kalium dan natrium dalam urin. Sebanyak 24 ekor tikus dibagi menjadi empat grup; kontrol negatif (akuades), kontrol positif (furosemide), dosis ekstrak 1 (250mg/kg BB) dan dosis ekstrak 2 (500mg/kg BB). Ekstrak diberikan secara peroral selama 7 hari. Aktivitas diuretik pada kelompok ekstrak dan kontrol positif pada jam pertama (P>0.05) dan jam keenam (P<0.05). Volume urin kumulatif dosis 1 mendekati furosemide. Pengukuran mineral urin menunjukkan hasil kehilangan mineral natrium dan kalium pada kelompok ekstrak lebih kecil dibandingkan kelompok referensi. Hasil dari penelitian ini menunjukkan bahwa dosis efektif ekstrak Kumis Kucing adalah 250 mg/kg BB pada jam ke-2 dan menunjukkan kehilangan mineral dalam urin yang lebih kecil.

Aktivitas Diuretik dan Analisa Mineral Urin Perlakuan Ekstrak Tanaman Kumis Kucing (Orthosiphon Stamineus Benth) pada Tikus Jantan Rini Madyastuti; Ietje wientarsih; Setyo Widodo; Erni H Purwaningsih; Eva Harlina
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Tanaman Kumis Kucing merupakan salah satu tanaman yang memiliki banyak keuntungan dan sudah digunakan sejak dahulu dalam upaya menjaga kesehatan. Tujuan dari penelitian ini adalah mengevaluasi aktivitas diuretik dari penggunaan ekstrak Kumis Kucing dan mengukur kadar kalium dan natrium dalam urin. Sebanyak 24 ekor tikus dibagi menjadi empat grup; kontrol negatif (akuades), kontrol positif (furosemide), dosis ekstrak 1 (250mg/kg BB) dan dosis ekstrak 2 (500mg/kg BB). Ekstrak diberikan secara peroral selama 7 hari. Aktivitas diuretik pada kelompok ekstrak dan kontrol positif pada jam pertama (P>0.05) dan jam keenam (P<0.05). Volume urin kumulatif dosis 1 mendekati furosemide. Pengukuran mineral urin menunjukkan hasil kehilangan mineral natrium dan kalium pada kelompok ekstrak lebih kecil dibandingkan kelompok referensi. Hasil dari penelitian ini menunjukkan bahwa dosis efektif ekstrak Kumis Kucing adalah 250 mg/kg BB pada jam ke-2 dan menunjukkan kehilangan mineral dalam urin yang lebih kecil.

Hemogram dan Respon Jaringan Hernia Insisional Babi Pascaterapi Mesh Bedah Polipropilen dengan atau tanpa Asam Hyaluronat Heryudianto Vibowo; . Gunanti; Eva Harlina
Acta VETERINARIA Indonesiana Vol. 7 No. 2 (2019): Juli 2019
Publisher : IPB University

Penggunaan mesh menjadi metode yang penting untuk terapi perbaikan hernia abdominalis karena dapat menurunkan angka kejadian hernia berulang dibandingkan dengan penggunaan metode penjahitan. Tujuan penelitian ini untuk mempelajari gambaran darah dan respon jaringan hernia insisional babi yang diterapi dengan mesh komposit dan polipropilen dengan atau tanpa asam hyaluronat secara histopatologi. Penelitian menggunakan babi sebanyak 11 ekor berumur 3-4 bulan yang dibagi menjadi 3 kelompok. Kelompok pertama mendapatkan mesh komposit (Physiomesh®), kelompok kedua mendapatkan mesh polipropilen (Ultrapro®) yang diberikan asam hyaluronat (Guardic®), dan kelompok ketiga mendapatkan mesh polipropilen (Ultrapro®). Penelitian dimulai dengan menginduksi hernia setelah itu dilakukan pemasangan mesh, permanenan jaringan dan mesh, dan pembuatan preparat histopatologi. Hasil penelitian menunjukan tidak adanya perbedaan yang nyata pada gambaran darah complete blood count antar kelompok. Hasil penelitian gambaran sel radang di jaringan juga tidak menunjukan perbedaan yang nyata kecuali hasil pengujian paired T-Test untuk makrofag pada kelompok mesh polipropilen. Sedangkan pada pengujian One-Way Annova tidak memberikan hasil yang berbeda nyata. Hasil penelitian ketebalan jaringan juga tidak menunjukan adanya perbedaan yang nyata antar kelompok. Dengan demikian pemberian asam hyaluronat tidak memberikan dampak negatif terhadap jaringan.

PAT-3 In Vitro Growth Inhibition Activities of Natural (nCaIFN) and Recombinant (rCaIFN) Canine Interferons on Three Different Tumor-Derived Cell Lines Bambang Pontjo Priosoeryanto; Rizki Rostantinata; Eva Harlina; Lina Noviyanti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Recently, from many kinds of diseases one could be counted is a tumor disease. Tumor is a degenerative disease that involved many biological pathways within the host. A tumor or neoplasm can be defined as a disturbance of growth characterized by excessive, abnormal and uncontrolled proliferation of transformed or altered cell(s) at one or more primary points within the host, and frequently at one or more metastatic sites (Priosoeryanto, 1994).The treatment of tumor is mostly by medical surgery which usually combined with chemotherapeutic agent(s), unfortunately the using of chemotherapeutic agent can develop a seriously side effect to the treated-patients. Biological substances like interferon (IFN) known as anti-virus agent is also one of promising candidate for treating or preventing of tumor disorders.Feline and canine squamous cell carcinoma is one of tumor type that often found in cat and dog, and mostly develop to a very aggressive disorder. The using of in vitro cell culture is a way to avoid the use of live animals on the study of tumors especially the study of antitumor agent due to can mimic the in vivo condition. The aim of the present study is to examine the growth inhibition activity of natural and recombinant canine interferon in order to find the suitable biological substances for combating tumor disorder especially in the field of veterinary medicine and also as an information for the development of tumor treatment in the human medical side.

PAT-8 Antiproliferation Activity of Keladi Tikus (Typhonium flagelliforme) Leaves Ethanol Extract on MCA- B1 and MCM-B2 Tumor – Derived Cell Lines In Vitro Riski Rostantinata; Bambang Pontjo Priosoeryanto; Eva Harlina; Waras Nurcholis; Lina Noviyanti; Rachmi Ridho
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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INTRODUCTIONTumor is a degenerative disease as the second agent cause of death in human. This disease is caused by disturbances of cell’s growth that show alteration and uncontrolled of cells proliferation. The uncontrolled of cells proliferation is also accompanied by penetration of that cells into others tissue and develop on it. Removed of tumor is usually done by operation or chemotherapy. Alternative treatment that can be used is herbal treatment. Typhonium flagelliforme known as “Keladi Tikus” in Indonesia; is known has a chemopreventive effect (2). Other researchers also show flavonoid glucoside of T. flagelliforme (1) can induce the activity of apoptosis in colon cancer cells (5). The aim of this study is to examine the in vitro anti-proliferation activity of Typhonium flagelliforme leaves ethanol extract on MCA- B1and MCM-B2 cell line.

Aktivitas Antitumor Ekstrak Etanol Daun Keladi Tikus (Typhonium flagelliforme) secara In Vivo pada Mencit Ardi Sandriya; Bambang Pontjo Priosoeryanto; Gunanti Gunanti; Eva Harlina; Riski Rostantinata; Lina Noviyanti Sutardi; Rachmi Ridho
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk mempelajari efektivitas ekstrak etanol daun keladi tikus (Typhonium flagelliforme) untuk terapi penyakit tumor. Penelitian ini menggunakan enam ekor mencit betina berumur lima minggu dengan bobot badan 20-25 g yang telah diinduksi karsinogen 7.12- dimetilbenz(?)anthracne (DMBA). Mencit dibagi menjadi dua kelompok perlakuan masing-masing kelompok terdiri dari tiga ekor mencit yaitu kelompok yang diberi plasebo (KK) dan kelompok yang diberi ekstrak etanol daun keladi tikus (KE). Kelompok KK diberi plasebo berupa aquabides. Kelompok KE diberi ekstrak etanol daun keladi tikus dengan dosis 120 mg/kg BB. Pengamatan dilakukan secara makroskopik dan mikroskopik. Massa tumor dari kedua kelompok mencit dikoleksi dan difiksasi dalam neutral buffered formalin (NBF) 10%. Kemudian dibuat preparat histopatologi dan diwarnai pewarna Hematoksilin-Eosin (HE). Hasil penelitian menunjukkan gambaran makroskopik permukaan kulit pada daerah tumbuhnya tumor mengalami alopesia, tumbuh benjolan berwarna kemerahan dan keropeng. Hasil pengukuran bobot badan mencit kelompok KK mengalami penurunan, sedangkan berat badan mencit pada kelompok KE mengalami kenaikan setiap minggunya. Diameter tumor kelompok KK mengalami peningkatan, sedangkan diameter tumor pada kelompok KE mengalami penurunan pada minggu ke empat pemberian bahan uji. Gambaran mikroskopik menunjukkan benjolan massa tumor yang terbentuk terdiri atas tiga jenis tumor yaitu anaplastic carcinoma (16,7%), fibrosarcoma (16,7%) dan squamous cell carcinoma (66,6%). Ketiga tumor digolongkan dalam diferensiasi baik 33,4%, diferensiasi sedang 33,4%, diferensiasi buruk 16,7%, dan tidak berdiferensiasi 16,.7%. Laju pertumbuhan tumor selaras dengan tingkat diferensiasi. Tumor yang bermetastasis sebanyak 50% dan tidak bermetastasis sebanyak 50%. Rataan indeks mitosis dan angiogenesis terjadi penurunan yang nyata (p<0.05) pada kelompok mencit yang diberi ekstrak etanol daun keladi tikus dibandingkan kelompok kontrol. Disimpulkan bahwa ekstrak etanol daun keladi tikus menurunkan pertumbuhan tumor dan dapat digunakan untuk terapi tumor.

GAMBARAN DARAH DAN KALSIUM DOMBA LOKAL (Ovis aries) YANG DIIMPLANTASI BIFASIK KALSIUM FOSFAT 70/30 SCAFFOLD 3D BERPOROS Dwi Utari Rahmiati; Gunanti Gunanti; Eva Harlina; Kiagus Dahlan
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this research is to study the biocompatibility biphasic calcium phosphate with alginate combination by evaluating blood and calcium concentration. This study used three male sheeps, age 1.5 year old with an average weight of 20 kg. Anaesthesia is using an intravenous anaesthetic combination of 10% ketamine HCl (2 mg / kg BW) and 10% xylaxine HCl (0.1 mg / kg BW). The implant material is grown on the right sheep tibia. As a control, the left sheep tibia was made of defects but not implanted. The incision orientation is the straight line of the tibial femoral joint at medial, 3.6 cm from caudal to cranial and perpendicular from that point 5.7 cm to the distal direction. The defect is made using a bone drill to make a hole according to the size of the implant (diameter 0.8 cm and 0.4 cm long). Blood parameters (erythrocytes, hemoglobin (Hb), hematocrit (Hct), total leukocytes and differentiation of leukocytes; neutrophils, eusinophils, basophils, lymphocytes and monocytes) and calcium evaluations were performed on days 0, 7, 30, 60 and 90 post implantation. Based on complete blood count no significant of blood parameters. Based on the calcium value, there are significant values on the 30th and the 90th. Blood calcium concentration were decreased from day 0 to 90.

Aktivitas Ekstrak Etanol Daun Alpukat Terhadap Zat Nefrotoksik Ginjal Tikus (ACTIVITIES STUDY OF ETHANOL EXTRACTS OF AVOCADO LEAVES (PERSEA AMERICANA MILL) TO NEPHROTOXIC COMPOUND OF RAT’S KIDNEY) Ietje Wientarsih; Eva Harlina; Rini Madyastuti Purwono; Ikrar Trisnaning Hardi Utami
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Based on WHO’s data, about 80% peoples in the word use plant’s extract as herbal medicine. Avocadoleaves has used as herbal medicine which is works as diuretikum, reduce stone in renal, and cure sprue.The purpose of this research is to study activities of ethanol extract of avocado leaves to ethylene glycolinduction on rat’s kidney by histopatologically. This experiment was continuing Adha’s research (2009)with research design as follows: twenty male rats were divided into 4 groups, negative control group (KN),positive control group (KP), treated group I by ethanol extract of avocado leaves 100 mg/kg BW (E100), andtreated group 2 by 300 mg/kg BW (E300). Sampling kidney were fixed in BNF 10% for histopathologicalslide and stained by Hematoxylin-Eosin. The kidney histopathological changes were edema of glomerulus,and protein deposit in the lumen, hyalin droplet and necrotic tubules. The results showed that ethanolextract of avocado leaves can’t significantly to reduce glomerulus edema with percentage cases 31.9% and33.5% for E100 and E300 and tend not to reduce the occurrence of protein deposits in the lumen of thetubule. Ethanol extract of avocado leaves tend to reduce the incidence of tubular necrosis and it wassignificantly different from the KP group (p <0.05). It is caused by flavonoid in etanol extract of avocadoleaves works as diuretikum and antioxidant.

Infusum Daun Alpukat Sebagai Inhibitor Kristalisasi Kalsium Oksalat pada Ginjal (THE AVOCADO LEAVES INFUSUM AS INHIBITOR ON RENAL CALCIUM OXALATE CRYSTALIZATION) Rini Madyastuti; Setyo Widodo; Ietje Wientarsih; Eva Harlina
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Urine crystal is a crystal nucleus which tend to form urine stone. The case of urine stone seems to beincreased every year. Crystallization could induce acute tubular necrosis which impact on renal dysfunction.The signs of this condition are high level of urea, creatinine and decrease glomerulus filtration rate. Theobjective of this research was to evaluate the effects of infusum Persea americana Mill as an inhibitorcrystallization which induced by ethylene glycol on white male rats. 20 male rats were divided into 4groups; K1 as negative group received only distilled water ad libitum, K2 as positive group receiveddistilled water containing ethylene glycol, K3 (dose 5%) and K4 (dose 10%) as treatment groups receivedwater containing ethylene glycol and avocado leaves infusion. Phytochemsitry screening of infusion avocadoleaves consisted of flavonoid, saponin, tanine and quinone. Result of analysis showed that the level ofureum and creatinine on K2 was higher than K3 and K4 group. The increased level could be inhibited byinfusion avocado leaves. The measurement of glomerular filtration rate in treatment groups wassignificantly different (p<0.05). Descriptive histopathology observation showed that renal lesio in grouptreatment (K3 and K4) were declined. Large crystal calcium oxalate on K2 group was observed by usingpolarized microscope, whereas small crystal calcium oxalate were seen in the infusion of avocado leavesgroups. These result showed the ability of infusion of avocado leaves as an inhibitor on the growth ofcrystallization calcium oxalate

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