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All Journal Media Veteriner Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Journal of Veterinary and Animal Sciences

I Gusti Ketut Suarjana

Laboratorium Bakteriologi Dan Mikologi Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali

Author-ID : 297707

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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KARAKTERISASI MOLEKULER DAN UJI PATOGENESITAS STREPTOCOCCUS PATOGEN ISOLAT ASAL BALI I Gusti Ketut Suarjana; Widya Asmara
Buletin Veteriner Udayana Vol. 4 No.1 Pebruari 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The main of this study were characterized of muramidase released protein (MRP) andexctracellular factor (EF) as virulence factor of Streptococcus beta hemolityc of Bali islotesand its patogenecity on mice.The MRP was isolated from the cell walls bacteria withmuramidase (lysozyme) and EF was obtained from supernatant of bacteria precipated with70% ammonium sulphate and then dialysed. These protein were identified by usingsodium-dodecyl sulphate polyacrylamide gel electrophoresis ( SDS-PAGE). Isolates thatwas observed are five consist of three isolates from pigs and two isolates from monkeys.Pathogenecity test using 20 mices divided into four group. Group I inoculated with 0,1 mltodd-hewitt broth steril as negative control, group II inoculated with 0,1 ml inoculum ofbacteria of Streptococcus suis type 2 (strain D282) as positive control, group III inoculatedwith 0,1 ml Streptococcus beta haemolytic isolated from monkey and group IV inoculated with 0,1 ml Streptococcus beta haemolytic isolated from pig. The result of this studyshowed that all isolates were consist of eight protein bands of MRP and one EF of 110 kDamolecular weight. Eight protein MRP were 125 kDa, 76 kDa, 60 kDa, 57 kDa, 48 kDa, 45kDa, 30 kDa, and 28 kDa respectively. Each isolates had two major protein bands of MRP(76 kDa and 45 kDa).The patogenecity test in mice showed that the morbidity andmortality rates were 100% and 60% respectively. The prevalence of meningitis in mice are100%. Clinical sign were observed 30 hour post inoculated (pi) whereas mice found deathstarty 48 pi.

TOTAL BAKTERI SAPI BALI PADA BERBAGAI UMUR DAN LOKASI PETERNAKAN DI NUSA PENIDA chyntia nirmalasari mantrawan; I Nengah Kerta Besung; I Gusti Ketut Suarjana; Ni Ketut Suwiti
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aims were to determine the total number of bacteria at maturity level and farm locations. As many as 24 catlle fecal samples were examined using the casting method. This study used a Randomized Complete Block Design of 2 x 3 factorial consist of two farm locations (highlands and lowlands) and three levels of cattle maturity (calves, heifers, adult). The total bacteria were analyzed using Analysis of Variance and continued by Least Significant Difference Test. The results of the total number bacteria isolated from feces adult 724.6250 x 109 CFU/g higher than heifers 667.5000 x 109 CFU/g and calf 499.5000 x 109 CFU/g. Total number of bacteria isolated from feces of bali cattle from highland 629.4167 x 109 CFU/g is not showed significant different compare to bali cattle from lowland 631.6667 x 109 CFU/g.

KUALITAS AIR MINUM TERNAK AYAM PETELUR DI DESA PILING KECAMATAN PENEBEL KABUPATEN TABANAN DI TINJAU DARI JUMLAH BAKTERI COLIFORM I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 1 No. 2 Agustus 2009
Publisher : The Faculty of Veterinary Medicine, Udayana University

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A study was carried out to evaluate the water quality in layer farming at Piling Village,Distric and Regency of Tabanan, based on number of Coliform bacteria. The study wasblockrandomized design was asigned in this study. Water samples were collected fromthree sourcess, river, water reservoir and drinking water of pen.Samples were collectedin10 time.The Coliform analysed by most probable number (MPN) Method with nine tubeseries.The result of this study showed that number of Coliform in river, reservoir and place ofdrinking water were 11240 MPN/100 ml, 19800 MPN/100 ml and 35600 MPN/ 100 mlrespectively. Statisticcaly of this study showed amount of Coliform in drinking water offarm layer significant highest (P<0,01) than those reservoir and river. Amount of Coliformin reservoir significant highest (P<0,01) than those river.

Seroepidemiologi Septichaemia Epizootica Berdasarkan Jenis Kelamin pada Sapi Bali di Sumbawa I Nengah Kerta Besung; I Gusti Ketut Suarjana; Ketut Tono PG; Ni Ketut Suwiti
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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This study aims to determine the distribution map Septicaemiaepizootica (SE) by gender in Dompu, Bima and Sumbawa. A total of 240 serum samples derived from three districts in West Nusa Tenggara used the study sample. Serum was tested by ELISA (Enzyme Linked Immunosorbent Assay). Data samples positive and negative SE, presented descriptively. The results showed that seropositive SE at 18% bulls and cows by 12%. It can be concluded that the incidence of SE in the bulls higher than cows.

Perbandingan Jumlah Bakteri Non-Coliform pada Feses Sapi Bali Berdasarkan Tingkat Kedewasaan dan Tipe Pemeliharan Kadek Andre Sulaksana; I Gusti Ketut Suarjana; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 9 No. 2 Agustus 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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This study aim was to determine the effect of maintenance type and maturity level of bali cattle to the total of non coliform bacteria in bali cattle. A total of 24 samples of bali cattle’s feces were used. This study was a Factorial 2 x 3 of group-randomised design, contained of two type maintenance (TPA Suwung and Sobangan) and three levels of cattle maturity (calves, heifers, and adult). The groups were based on time sampling. The data then analyzed using Analysis of Variance Test and continued by using Least Significant Different test. The results showed that the amount of non-coliform bacteria in adult bali cattle feces were higher then heifers and calves. Total of non coliform bacteria in bali cattle in landfill was higher then the cattle that reared in captivity in Bali Cattle Breeding Center, the Sobangan village of Mengwi Badung regency.

Isolasi dan Identifikasi Bakteri dari Susu Kambing Peranakan Etawa Terindikasi Mastitis Klinis di Beberapa Kecamatan di Kabupaten Banyuwangi M Hasan Isnan; Ketut Tono Pasek Gelgel; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The purpose of this study was to determine the types of bacteria as a cause of clinical mastitis in goats Peranakan etawa in Banyuwangi. In this study, the sample used goat milk peranakan etawa in District Pesanggaran, Rogojampi, Songgon and Srono in Banyuwangi. As many as 11 samples were taken then analyzed in Laboratory Microbiology, Faculty of Veterinary Medicine Udayana University. Phase isolation and identification of samples were done to identify the bacteria that cause clinical mastitis through several stages of growth on Sheep Blood Agar (SBA), Gram stain, catalase test, test oxidase, growth in the TSIA and continued test Indol, Methyl Red, Voges Proskauer and citrate (IMViC ). Based on the results, namely mastitis-causing bacteria; Staphylococcus sp, Pseudomonas sp, Neisseria sp, E.coli, Corynebcaterium sp and Listeria sp. The conclusion of this study disease mastitis in goats Peranakan etawa in the District of Bayuwangi caused by Staphylococcus sp, Pseudomonas sp, Neisseria sp, E.coli, Corynebcaterium sp and Listeria sp.

Analisis Marka Gen Patogenik iutA Escherichia Coli Penyebab Colibasilosis pada Ayam Buras Kadek Satria Adi Marhendra; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Colibacillosis is an infectious disease in chickens caused by Avian Pathogenic Escherichia coli (APEC). The ability of APEC to cause disease depends on many pathogenic factors, one of which is the iutA pathogenic gene. This study was purposed to determine the DNA sequence of the APEC iutA gene in Bali and its kinship with the iutA gene from other countries. Two APEC isolates from free-range chicken in Tabanan dan Badung has been used. The isolates have been purified and were available at the Bacteriology Laboratory of Veterinary Medicine Faculty, Udayana University. Chelex 10% was used for DNA isolation. DNA amplification using published DNA primer has been conducted with the polymerase chain reaction (PCR) method. The PCR product was sequenced at Malaysia, First Base Laboratories using Sanger’s Dideoxy Nucleotide Termination method. The iutA gene of both isolates can be analyzed and have 250 bp in length. Both were 100% homologous. A phylogenic test using 58 DNA sequences of iutA gene from Escherichia coli and other bacteria in the world was conducted in MEGA 5.2. All data have 43 polymorphic sites of nucleotide acid and 13 polymorphic sites of amino acid. The iutA gene from Bali is in one group, with the iutA gene from Brazil (KP657535) in 2011, Germany (LT599825) in 2016, and China (CP033635) in 2016. This gene can be used as a pathogenic marker of APEC in Indonesia.

Pola Kepekaan E coli Yang Diisolasi Dari Feses Broiler Penderita Diare Terhadap Sulfametoksazol, Ampisilin Dan Oksitetrasiklin (SENSITIVITY PATTERN OF E. coli ISOLATED FROM PATIENTS WITH DIARRHEA BROILER FAECES AGAINST SULFAMETOKSAZOLE, AMPICILLIN I Wayan Aris Suharsa; I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel
Buletin Veteriner Udayana Vol. 7 No. 2 Agustus 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Broiler merupakan jenis ayam yang paling cepat berproduksi dan dagingnya memiliki mutu yang tinggi dibandingkan dengan jenis ayam ras lainnya. Penelitian ini bertujuan untuk mengetahuikepekaan bakteri Escherichia coli (E. coli) sebagai penyebab kolibasilosis terhadapsulfametoksazol, ampisilin, dan oksitetrasiklin pada peternakan broiler di Banjar Tangkub, DesaPayangan. Bakteri E. coli diisolasi dari 30 sampel feses broiler penderita diare diuji kepekaanyadengan menggunakan metode Kirby-Bauer dan dianalisis secara deskriptif. Hasil penelitianmenunjukkan bahwa sebanyak 25 isolat resisten (83,33%), satu isolat intermediet dan sisanyaempat isolat sensitif terhadap antibiotika oksitetrasiklin. Sedangkan terhadap antibiotika ampisilin,sejumlah 24 isolat (86,67%) menunjukkan resisten dan sisanya yakni empat isolat sensitif terhadapantibiotika tersebut. Terhadap sulfametoksazol, kuman E coli yang diisolasi menunjukkan 29 isolatresisten (96,67%) dan satu isolat sensitif. Berdasarkan hasil penelitian dapat disimpulkan bahwapenggunaan sulfametoksazol, ampisilin dan oksitetrasiklin sebaiknya dihindari.

Deteksi Bakteri Staphylococcus sp. dari Saluran Pernapasan Babi Makselina Ayu Dwi Purwanti; I Nengah Kerta Besung; I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

This research was conducted for the detection of Staphylococcus sp. in the respiratory tract of pigs. Samples differentiated by sex (male pigs and female pigs). A total of 100 nasal swab samples were collected aseptically from pigs showing symptoms of respiratory disease, from pig farms in the area of Badung and Tabanan. All samples were cultured on to Blood Agar for 18-24h at 37°C. Suspected colonies were then Gram staining, primary tests (tested for catalase and oxidase) and series of biochemical tests. Statistical analysis (Chi-square) was performed to explore the differences in positive samples numbers of Staphylococcus sp. isolated between male and female pigs. Of the 100 samples, seven positive samples of Staphylococcus sp. (six of the boars and one from the female pig). Statistically, the number of boars infected with Staphylococcus sp. higher (P<0.01) than with female pigs Staphylococcus species isolated were: Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus, respectively.

Enterobacter sp. pada Sapi Bali Menurut Geografis dan Tingkat Kedewasaan serta Pola Kepekaannya Terhadap Antibiotika I Wayan Suarnata; I Gusti Ketut Suarjana; Aida Louise Tenden Rompis
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

An observational study in order to determine the distribution Enterobacter spin Bali cattle at different geographical area (highland and lowland) and according to the animal maturity (calves, heifers, adult) have been undertaken. Isolation and identification of bacteria based on the method of Carter and Cole with slightly modification and the antibiotic sensitivity test were determined using the disc diffusion method by Kirby and Bauer. The Enterobacter sp. was isolated from 16 (13.3%) out of the 120 nasal swab samples,where 8 (6.7%), 6(5.0%), and 2 (1.7%) was isolated from calf, heifers, and adults, respectively. Meanwhile, 6 (5.0%) Enterobacter sp. was isolated from the highland and the remains (8.3%) from the lowland. Antibiograms showed that 13 of the 16 isolates (81.2%) weresensitive to sulfamethoxazole and the remains (18.8%) intermediate. While 12 (75.0%), 1 (6.2%), and 3 (18.8%) isolates weresensitive, intermediate, and resistant to ampicillin, respectively. To oxytetracycline 8 (50.0%) isolates were sensitive, 4 (25.0%) isolates were intermediate, and 4 (25.0%) isolates were resistant. Generally, the Enterobacter sp isolates were sensitive to all the antibiotics tested.

Co-Authors Agnes Endang Tri H Ahmad Nuzuludin Kadri, Ahmad Nuzuludin Aida Lousie Tenden Rompis Anak Agung Gede Agung Ananta Kusuma ANITA KAROLINA SIMAMORA BERATA BERATA Bianca Violanda Junus Bolla, Nelci Elizabeth chyntia nirmalasari mantrawan Daniel Opristanta Barus Danu Suprayogo Dwi Arso Purba Dwi Astalia Zuanita Eggy Hidta Lusandika Fanayoni, Aditana Genta Dhamara Adam Putranto HARDIANTO - HERTATI ANRIANI LUBIS I Gede Eka Chandrawan I Gusti Ngurah Kade Mahardika I Kadek Juli Handriana I Ketut Berata I Ketut Suada I Made Adhi Kusuma Dwipayana I Made Kardena I Made Merdana I Made Sukada I Nengah Kerta Besung I Putu Yasmanta Primarta Putra I Wayan Aris Suharsa I Wayan Suarnata I.A.P. Apsari IB. KADE SUARDANA Ida Bagus Oka Winaya Ike Siwi Widyaningtyas Indra Manik Pradipta Iwan Harjono Utama JERRY BIROWO K. Tono P.G Kadek Andre Sulaksana Kadek Satria Adi Marhendra KARTINI HUTASOIT Ketut Tono Pasek Gelgel Leny Beatry Veronica Luh Made Sudimartini M Hasan Isnan Made Deddy Dharmana Putra Made Tubagus Dharmajaya Magfirah Syahruddin Makselina Ayu Dwi Purwanti MARGARET ANASTASIA MANULLANG Marlissa, Faccettarial Cylon Marchel MAS DJOKO RUDYANTO Matilda Krisnawati MUHAMMAD RHIYAN SAKTI N.K. Suwi N.N. Suryani Nazara, Agustina Lesmauli Ni Kadek Meita Swandewi Ni Ketut Suwiti Ni Putu Nicky Mirahsanti Nyoman Anandiya Ramaditya P. Suastika Paramita, Putu Wahyuni Pratanto, Aditya Putri Udayani Putu Henrywaesa Sudipa Ratna Pandu Finata Rusmin Indra Septian Naria Rusmaniarno Sousa, Rojelio Dias Trindade Tania Ria Gunawan Trisno, Komang Voni Cornelia Br Sembiring Wati, Ni Komang Desi Yena Widya Asmara Yuniarti Sasmita Yusmaniar Galuh Adi Luhung

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