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KARAKTERISASI TRICHODERMA HARZIANUM ASAL LAHAN GAMBUT SEBAGAI AGENS ANTAGONIS TERHADAP PENYEBAB PENYAKIT BUSUK PANGKAL BATANG SAWIT SECARA IN VITRO Nandung, Elsy; Suswanto, Iman; Ramadhan, Tris Haris
Perkebunan dan Lahan Tropika Vol 8, No 2 (2018): Perkebunan dan Lahan Tropika
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.823 KB) | DOI: 10.26418/plt.v8i2.29798

Abstract

T. harzianum  merupakan agen antagonis yang digunakan untuk pengendalian hayati. Penelitian ini bertujuan mengetahui keragaman cendawan dari beberapa jenis vegetasi di lahan gambut,  mengetahui kemampuan daya hambat dan sifat-sifat lain T. harzianum penentuan sifat yang dapat digunakan sebagai penciri T. harzianum sebagai agens pengendali busuk pangkal batang, dan kemampuan mendegradasi kitin sebagai salah satu komponen dinding sel Ganoderma spp.. Pengujian ini dilakukan melalui hasil isolasi T. harzianum dari beberapa areal, inkubasi menggunakan media PDA kemudian dilakukan pencirian berdasarkan uji antagonis, uji pertumbuhan, morfologi dan mekanisme penghambatan, uji kitinase dilakukan dengan menginkubasi cendawan  T. harzianum  pada media kolodial kitin diamati zona bening setiap hari.   Keragaman cendawan dari berbagai wilayah relatif sama. Agen pengendali Ganoderma spp. seperti busuk pangkal batang banyak ditemukan pada wilayah yang ditumbuhi cabai dan hutan belukar. Pencirian T. harzianum terbaik berdasarkan tingginya daya hambat, kerapatan spora dan mekanisme penghambatan. Mekanisme penghambatan bersifat hiperparasit mampu menekan pertumbuhan Ganoderma spp. melalui degradasi kitin.Kata kunci : Busuk Pangkal Batang, Gambut, Pencirian T. harzianum, Sawit
UJI PATOGENISITAS NEMATODA PATOGEN SERANGGA (Steinernema carpocapsae) DARI TANAH GAMBUT TERHADAP RAYAP TANAH (Coptotermes curvignathus) Paster, Ari; Hendarti, Indri; Ramadhan, Tris Haris
Perkebunan dan Lahan Tropika Vol 8, No 2 (2018): Perkebunan dan Lahan Tropika
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.25 KB) | DOI: 10.26418/plt.v8i2.29797

Abstract

Rayap tanah (Coptotermes curvignathus) merupakan salah satu hama yang menimbulkan kerugian ekonomis yang sangat besar karena banyak menyerang tanaman pertanian sehingga menyebabkan tanaman mati dan produksi menurun. Salah satu cara pengendalian yaitu secara biologis menggunakan nematoda patogen serangga (Steinernema carpocapsae). Penelitian ini bertujuan untuk melihat daya patogenisitas S. carpocapsae dari isolat tanah gambut terhadap rayap tanah (C. curvignathus). Penelitian dilaksanakan di Laboratorium Hama Tumbuhan, Fakultas Pertanian, Universitas Tanjungpura Pontianak. Rancangan yang digunakan Rancangan acak kelompok (RAK) dengan menggunakan satu perlakuan dan 6 taraf  konsentrasi S. carpocapsae (25 ji/ml, 50 ji/ml, 100 ji/ml, 200 ji/ml, 400 ji/ml, dan 800 ji/ml) dengan 5 kali pengulangan. Pengujian patogenisitas dilakukan terhadap C. curvignathus kasta pekerja. Kemampuan patogenisitas S. carpocapsae diukur berdasarkan mortalitas, jumlah S. carpocapsae yang keluar, periode letal dan virulensi. Hasil pengujian menunjukan bahwa S. carpocapsae mampu menyebabkan mortalitas sebesar 100% pada perlakuan 800 ji/ml dalam 96 jam setelah inokulasi. Priode letal yang dihasilkan sebesar 40,68 dan Virulensi sebesar 0,024. Jumlah S. carpocapsae yang dihasilkan tidak berbeda nyata antara satu perlakuan dengan perlakuan lainnya dikarenakan C. curvignathus merupakan serangga uji yang memiliki tubuh ukuran kecil antara 4,5-5,0 mm. Sehingga tiap perlakuan konsentrasi S. carpocapsae yang diinokulasikan memiliki kapasitas ruang yang  sama untuk perkembangannya.Kata kunci : Inokulasi, Mortalitas, Priode letal, Virulensi
ISOLASI ENTOMOPATOGEN LAHAN GAMBUT DI KALIMANTAN BARAT DAN DETERMINASI VIRULENSINYA SEBAGAI MATERIAL BIOINSEKTISIDA Ramadhan, Tris Haris; Hernowo, Kukuh
Perkebunan dan Lahan Tropika Vol 2, No 2 (2012): PERKEBUNAN DAN LAHAN TROPIKA
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.347 KB) | DOI: 10.26418/plt.v2i2.3515

Abstract

Entomopatogen merupakan salah satu unsur penting dalam pengendalian hayati dan akan semakin baik jika dikembangkan secara khusus melalui ekplorasi dan pengujian di skala laboratorium. Diharapkan dari penelitian didapatkan entomopatogen lahan gambut yang potensial. Entomopatogen diisolasi dengan cara mengambil cuplikan tanah dan dipanaskan selama 60 menit pada suhu 85 oC. Sedangkan jamur patogen diisolasi dengan teknik mengencerkan cuplikan tanah lalu diinkubasi dalam media agar. Pengujian terhadap serangga di laboratorium dengan metode celup pakan pada media yang mengandung entomopatogen. Kematian serangga akibat perlakuan diamati sampai ulat uji menjadi imago. Hasil pengujian didapatkan tingkat kematian ulat yang diperlakukan dengan bakteri entomopatogen sebesar 33-40 persen sedangkan dengan perlakuan jamur entomopatogen kematian ulat 20-33,3 persen. Kematian pada perlakuan dengan bakteri terjadi pada fase larva-pupa sedangkan perlakuan jamur pada fase larva. Kematian serangga uji terjadi setelah perlakuan 6-18 hari. Isolat bakteri dan jamur entomopatogen dari lahan gambut memberikan harapan untuk dikembangkan sebagai bioinsektisida Kata kunci: Entomopatogen, isolasi, Spodoptera litura
Keragaman Vegetasi dan Identifikasi Fungi Mikoriza Arbuskula (FMA) Pada Lahan Bekas Tambang Riko Prasetyo; Iwan Sasli; dan Tris Haris Ramadhan
Indonesian Journal of Agronomy Vol. 47 No. 2 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.781 KB) | DOI: 10.24831/jai.v47i2.25050

Abstract

Revegetation of former gold mining land is expected to restore soil fertility. One of the ways to use the gold-mined land as agricultural land is by utilizing the symbioses of arbuscular mycorrhizal fungi (AMF) is expected to accelerate the revegetation process. The aim of the study was to analyze vegetation and  AMF exploration in secondary forest land (SF) and former gold mining land (FGML) in the Mandor District  . The research stages were conducting the plant identification and the exploration of AMF at the laboratory of Pests and Diseases of Faculty of Agriculture, University of Tanjungpura. The research was conducted for ± 2 months starting from February to April 2018. The parameter of the observation was done by finding the summed dominance ratio (SDR) value, diversity index (DI), the density of spore (DS), relative frequency (RF), the identification of spore, and the analysis of plant root infection. The research finding showed that from the calculation of SDR, there were two dominant plants i.e. Paspalum conjugatum and Melastoma candidum. The calculation result of DI was categorized as ‘moderate’ in three locations of observation. The observation of DS in SF obtained the highest value of 750 spores/100 grams of soil. The total genus Glomus in SF and FGML > 10 years had a higher value than genus Acaulospora. In the three locations of observation, it had been obtained the genus Glomus and Acaulospora, while there was an infection at the observation of staining roots; it was characterized by the existence of arbuscular, vesicles, and external hyphae.
A Uji Amelioran Organik Asal Limbah Pertanian pada Pertanaman Sawi Hijau (Brassica juncea L.) di Tanah Gambut Armiyarsih; Iwan Sasli; Tris Haris Ramadhan
Indonesian Journal of Agronomy Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (591.987 KB) | DOI: 10.24831/jai.v48i3.32997

Abstract

The use of agricultural waste as an organic ameliorant can reduce the dependence on the use of synthetic chemical fertilizers on the cultivation of mustard on peat soils. The research objective was to determine the best effect of the compost dosage of oil palm empty bunches (TKKS) and banana trunk waste on the growth and yield of mustard in peat soil. The location of the study was located in Baning Village, Sintang District, from April to June 2020. The research used a factorial completely randomized design. The first factor is the usage of 5 compost levels of TKKS which are t0 (without compost), t1(5 tons ha-1), t2(10 tons ha-1), t3(15 tons ha-1),t4(20 tons ha-1). The second factor was the dosage of banana trunk compost with 4 levels of p0(without compost), p1(10 tons ha-1), p2(20 tons ha-1), p3(30 tons ha-1). Treatment interactions significantly increased plant height, leaf number, leaf area, plant fresh weight, plant dry weight, net assimilation rate, and plant growth rate. The N, P, and K content in the soil increased by a percentage of 53.85%, 409.90%, and 2,709% on the application of 15 tons ha-1 TKKS compost + 30 tons ha-1banana trunk and compost. This treatment combination was the best in this study for increasing the production of mustard greens in peat soil, indicated by the average of plant fresh weight of 176.71 g. Keywords: banana trunk, crop production, oil palm empty bunches
Pengaruh Jenis Mangsa dan Suhu pada Perkembangan Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae) dan Peranannya dalam Pengendalian Diaphorina citri Kuwayama (Hemiptera: Psyllidae) Tris Haris Ramadhan; Y. Andi Trisyono; Eddy Mahrub; Arman Wijonarko; Siti Subandiyah; George Andrew Charles Beattie
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.486 KB) | DOI: 10.22146/jpti.11874

Abstract

Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is the vector of citrus greening (Huanglongbing) bacterium and the most serious impediment to citrus culture. Classical biological control of this psyllid vector should contribute to suppress their population. This research was conducted to determine the performance of Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae) when they were fed with D. citri. The larval performance index of M. sexmaculatus on D. citri compared with Aphis craccivora Koch (Hemiptera: Aphididae) diet was 1.3.M. sexmaculatus fed with D. citri had lower fitness than those fed with A. craccivora as shown by longer larval stadium, lower adult dry weight, less number of egg produced and lower percentage of egg hatched. M. sexmaculatus grew best at the temperature of 27oC. Employing the exclusion procedure under field condition,M. sexmaculatus could reduce the population of D. citri up to 90%. These findings showed that theM. sexmaculatus could be a potential predator in reducing D. citri, particularly when the more preferred prey A. craccivora was not present.
PENGARUH PEMBERIAN KAPUR DAN PUPUK NPK TERHADAP PERTUMBUHAN DAN HASIL TANAMAN JAGUNG (Zea mays L.) PADA TANAH PODSOLIK MERAH KUNING Petrus Boni; Tris Haris Ramadhan; Tatang Abdurrahman
Jurnal Ilmiah Hijau Cendekia Vol 5 No 2 (2020): Jurnal Ilmiah Hijau Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/hijau.v5i2.1144

Abstract

Lahan kering PMK akan mampu menyediakan air dan hara yang cukup bagi tanaman bila ada perbaikan pada struktur dan kimia tanah. Penelitian ini dilakukan untuk mempelajari dan mengetahui interaksi pengaruh pemberian kapur dan pupuk NPK terhadap pertumbuhan dan hasil jagung di lahan kering serta diperolehnya dosis kapur dan pupuk NPK yang tepat dalam meningkatkan pertumbuhan dan hasil jagung di lahan kering. Penelitian dilaksanakan di Desa Amboyo Inti, Kecamatan Ngabang, Kabupaten Landak, Kalimantan Barat. Waktu pelaksanaan penelitian pada bulan Februari 2020 – Juni 2020. Penelitian ini menggunakan metode eksperimen lapangan dengan pola Rancangan Acak Kelompok Faktorial yang terdiri dari 2 faktor dengan 3 ulangan. Faktor pertama adalah dosis kapur (K) sebanyak 3 taraf yaitu 1 ton/ha, 2 ton/ha dan 3 ton/ha dan faktor kedua adalah dosis pupuk NPK (P) sebanyak 3 taraf yaitu 175 kg/ha, 350 kg/ha dan 525 kg/ha. Pengamatan tanaman meliputi 1). komponen pertumbuhan seperti tinggi tanaman, luas daun, indeks luas daun, laju pertumbuhan tanaman, 2). Komponen hasil meliputi indeks panen, bobot 100 biji , Panjang tongkol dan bobot kering biji jagung per petak. Komponen pertumbuhan yaitu tinggi tanaman, luas daun, indeks luas daun, laju pertumbuhan tanaman terjadi interaksi antara perlakuan dosis kapur dan dosis pupuk NPK. Komponen pertumbuhan generatif perlakuan kapur dan dosis NPK terjadi interaksi pada variabel bobot biji per petak dan bobot biji per tanaman sedangkan panjang tongkol, bobot 100 biji dan indeks panen tidak terjadi interaksi. Perlakuan dosis kapur 2 ton/ha dengan kombinasi pupuk NPK 350 kg/ha memberikan hasil tertinggi terhadap bobot biji kering jagung per petak sebesar 2139,67 gram. Kata Kunci : Kapur, NPK, Jagung, Podsolik Merah Kuning
Improved properties of Pepper (Pepper nigrum L.) using Mutagen Ethyl Methane Sulphonate (EMS) Iman Suswanto; Indri Hendarti; Tris Haris Ramadhan
Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Vol 20 No 2 (2022): Jurnal Agroqua
Publisher : University of Prof. Dr. Hazairin, SH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32663/ja.v20i2.3023

Abstract

The study aimed to determine the LC50 using EMS on pepper and obtain a better mutant than the parents. The material used was Indian variety pepper, and the chemical mutagen was Ethyl Methane Sulphonate (EMS). Two hundred Indian Cultivar pepper seeds obtained from community gardens were treated with EMS mutagen at a concentration of 0; 0.2; 0.4; 0.6; 0.8, and 1.0% with 3 hours of immersion. Observations consisted of germination, mutagen damage and agronomic characters. The LC50 calculation was obtained from the regression relationship of dead sprouts with several doses of EMS application which had the best fitting curve with the highest determination value (R2). The results showed that pepper germination was relatively low (62%). The impact of using EMS mutagens causes growth inhibition or even death of sprouts. The higher the concentration, the higher the mortality rate. The relationship between the concentration of mutagens and the mortality rate follows the polynomial equation y = 188.45x – 97.21x2 + 2.71 with a determination value of 99%. Based on the regression model, the LC50 value is 0.3%. In this study, the EMS concentration of 0.2% succeeded obtaining a better mutant pepper than the parental. indicated by increasing the length and width of the cotyledons, respectively, 20 and 24% greater than the control.
Penggunaan beberapa cendawan endofit untuk menekan penyakit layu pada jahe (Zingiber officinale Rosc.) di lahan gambut Iman Suswanto; Tris Haris Ramadhan
Jurnal Agrotek Ummat Vol 9, No 1 (2022): January
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jau.v9i1.6894

Abstract

Efforts to overcome R. solanacearum which causes bacterial wilt (BW) in ginger in peatlands are through a strategy to suppress the initial population. One of the efforts is to use endophytic fungi (CE) as biological control agents. This study aims to examine the use of several endophytic fungi isolates from pepper to suppress bacterial wilt disease and improve the growth of ginger. The treatment of CE biological agents in the form of Aspergillus spp., Fusarium sp., T. harzianum, and T. viride to suppress wilt disease in vivo and the use of biological agents as biofertilizers in the field. The source of the pathogenic bacterium P. solanacearum was obtained from samples of wilted rhizomes from community ginger field isolated on NA media. This study was arranged based on a completely randomized design with five replications. The research variables were wilt disease severity, plant height, number of leaves, number of stems, stem diameter & rhizome weight. The results showed that R. solanacearum caused symptoms after an incubation period of 9 days after inoculation. The four CE isolates were able to suppress the symptoms of ginger wilt in the range of 70-80%. The use of CE isolate was also able to improve the growth of ginger, but it has not been followed by an increase in the production of ginger rhizomes. It is suggested that the use of CE as a biofertilizer needs to be increased in the frequency of its application.