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Analysis of Low–level Cadmium Exposure Effects on HUVECs (Human Umbilical Vein Endothelial Cells) Cell Viability and Morphology Kristianningrum Dian Sofiana; Provisia Marthalita Y.W.; Khotimah Husnul; M Aris Widodo
Journal of Agromedicine and Medical Sciences Vol 5 No 1 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i1.9126

Cadmium is a heavy metal that could be found in daily life. This metal has a toxicity, could contaminate the environment, and affect human health. The main aim of this research was to find the effect of low concentration Cadmium exposure in acute time toward HUVECs cell morphology and viability.In a True experimental research with in vitro model using HUVECs cell, HUVECs cell was divided into four groups. One control group (without CdCl2 induction) and three treatment groups with CdCl2 induction with various concentrations, 0,153 µg/L, 1,53 µg/L and 15,3 µg/L. The trial was repeated five times for each group. Cell morphology was observed with an inverted microscope. Cell viability was examined by MTT assay. Data were analyzed using Kruskal Wallis statistical test and continue with the Man Whitney test. Correlation test was using Spearman.Morphology of treatment group HUVECs cell induced by CdCl2 concentration 15,3 µg/L looked significantly different compared with control group (p<0.05). Cell viability on group HUVECs induced by CdCl2 15,3 µg/L significantly different compared with the control group. The correlation test resulted R= -0,665 with probability 0.001 which means the higher concentration of CdCl2 the lower the viability of cells. Cadmium in low concentration induces cell morphology change and reduce cell viability. Keywords: HUVEC, cadmium, cell morphology, cell viability.

Comparison of The Number of Macrophage in Full Thickness Wound Incision between Merremia mammosa Extract Treatment and NaCl in Male Wistar Rats Fajar Kurniawan Hidayat; Ulfa Elfiah; Kristianningrum Dian Sofiana
Journal of Agromedicine and Medical Sciences Vol 1 No 1 (2015)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

The incidence of diabetes mellitus in Indonesia continues to increase. There are some complications in the diabetic condition, one of which is diabetic ulcer. Diabetic ulcers in diabetes patient can increase the risk of amputation and expensive treatment costs, so the alternative treatmeant such as Merremia mammosa wich has antiinflamatory and antidiabetic is needed. This study aimed to determine the comparison of the number of macrophage in the incisional wound in hyperglycemic male wistar rats between treatment with Merremia mammosa extract and NaCl. The in vivo test was done by creating wound incision on the mice backs and treated with gentamycin ointment 5% in a positive control group, NaCl in a negative control group and Merremia mammosa extract in a dose of 100m), 200mg, 400mg in treatment group. The result obtained by counting the number of macrophage in histopatholgy examination. The result showed the number of macrophage were 0,36 cells/field of view in a negative control group, 0,52 cells/field of view in a positive control group, 0,48 cells/field of view in all Merremia mammosa treatment groups. The data analysis showed no significant difference with p-value of 0,729. In conclusion, there was no significant difference between the used of Merremia mammosa extract and NaCl on full thickness incisional wounds of hyperglycemic male wistar rats. Keywords: Diabetes Mellitus, Merremia mammosa extract, incisional wound

Analisis Penggunaan Jumlah Bahan Aktif Pestisida dan Banyaknya Keluhan Masalah Kesehatan Pada Petani di Wilayah Agroindustri Jember Kristianningrum Dian Sofiana; Laksmi Indreswari; Jauhar Firdaus; Aris Prasetyo; Pulong wijang Pralampita; Supangat Supangat
Poltanesa Vol 23 No 1 (2022): Juni 2022
Publisher : P2M Politeknik Pertanian Negeri Samarinda

Pestisida dapat menyebabkan masalah kesehatan baik akut ataupun kronik. Latar belakang dari penelitian ini adalah Penggunaan pestisida di kawasan agroindustri Jember yang tidak dapat dihindari.. Paparan pestisida pada petani Jember, baik paparan langsung ataupun tidak langsung, diduga menjadi salah satu penyebab munculnya masalah kesehatan.. Keterkaitan antar penggunaan bahan aktif pestisida dan masalah kesehatan pada petani di wilayah agroindustri jember perlu diteliti. Penelitian ini bertujuan untuk menganalisis penggunaan jumlah bahan aktif pestisida dengan banyaknya masalah kesehatan pada petani di wilayah Agroindustri Jember. Penelitian ini menggunakan metode deskriptif observational dengan pendekatan cross-sectional. Populasi yang diambil adalah petani yang berada di kabupaten jember yang diwakili oleh 11 kecamatan. Besar sampel berjumlah 84 orang yang diambil dengan accidental sampling. Variabel yang diambil adalah jenis pestisida, jumlah bahan aktif yang dipakai, keluhan masalah kesehatan, Analisis menggunakan analisis unvaried dan bivariate menggunakan Chi square tes dengan p <0,05. Penggunaan bahan aktif yang digunakan selama penyemprotan mayoritas 1 bahan aktif 51,19% .Keluhan masalah kesehatan sebesar 52,38% dengan keluhan terbanyak adalah pusing sebesar 29,76%.Hasil Analisis jumlah bahan aktif saat penyemprotan dengan banyaknya keluhan kesehatan dengan menggunakan Chi Square tes, p= 0,009 dengan nilai p<0,05. Dari penelitian ini didapatkan ada hubungan penggunaan bahan aktif residu pestisida dengan keluhan masalah kesehatan pada petani di Jember. Petani yang terpapar kebanyakan tidak merasakan keluhan kesehatan.

PROTEIN PILI 96,4 kDa Klebsiella pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Laila Rizqi Kurniawati; M Ali Shodikin; Dini Agustina; Kristianningrum Dian Sofiana
Indonesian Journal for Health Sciences Vol 5, No 1 (2021): March
Publisher : Universitas Muhammadiyah Ponorogo

Klebsiella pneumoniae merupakan bakteri genus Klebsiella dalam famili Enterobactericeae. Klebsiella pneumoniae menyebabkan infeksi serius. Faktor virulensi penting dalam perlekatan awal bakteri untuk menginfeksi inang pili dan Outer Membrane Protein (OMP). Pili sebagai faktor virulensi dapat melekat kuat secara spesifik dan mampu menghindari pembersihan yang dilakukan oleh sel inang. Kemampuan adhesi suatu bakteri dipengaruhi oleh kemampuannya dalam mengagglutinasi eritrosit. Berdasarkan studi pendahuluan yang telah dilakukan, berat molekul protein pili yang muncul dari hasil elektroforesis SDS-PAGE yaitu 96,4 kDa memiliki pita tebal dan berkonsentrasi tinggi serta belum ada penelitian membuktikan bahwa protein pili 96,4 kDa merupakan protein hemaglutinin dan adhesin.Tujuan penelitian ini untuk mengetahui peran protein pili 96,4 kDa bakteri Klebsiella pneumoniae sebagai protein hemaglutinin dan adhesin. Penelitian ini dilakukan dengan mengisolasi protein pili Klebsiella pneumoniae kemudian dilakukan uji aglutinasi menggunakan sel eritrosit mencit dan uji adhesi menggunakan sel enterosit mencit. Hasil yang diperoleh dianalisis menggunakan Saphiro-Wilk. Hasil uji hemaglutinasi dengan menggunakan sel eritrosit mencit didapatkan titer hemaglutinasi paling tinggi untuk berat molekul protein pili 96,4 kDa adalah pada titer 1. Indeks adhesi dengan titer pengenceran memiliki hubungan yang bermakna yaitu 0,016 (p 0,05) dengan nilai r = -0,847, sehingga disimpulkan protein pili 96,4 kDa Klebsiella pneumoniae merupakan protein hemaglutinin dan adhesin.

REVIEW LITERATUR : MENDENGARKAN MUROTAL AL QURAN MENINGKATKAN KUALITAS TIDUR PADA LANSIA kristianningrum dian sofiana
Jurnal Pendidik Indonesia (JPIn) Vol 5, No 2: Oktober 2022
Publisher : Yayasan Pendidikan Intan Cendekia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47165/jpin.v5i2.405

AbstrakPenuan yang terjadi pada Lansia seringkali diikuti dengan perubahan pada kualitas tidur. Kualitas tidur ini menurun dikarenakan gangguan tidur seperti insomnia, gangguan nafas saat tidur, gangguan perilaku tidur,gangguan tidur REM.Problema gangguan tidur pada lansia ini bisa berakibat pada fungsi fisiologi tubuh maupun psikologi yang bisa mengakibatkan sakit fisik maupun mental.Tujuan penelitian ini adalah untuk mengetahui pengaruh terapi murotal al Quran dalam meningkatkan kualitas tidur pada Lansia.Penelitian ini menggunakan literature review dengan menggunakan database nasional dan international yakni Pubmed, Google Scholar dengan rentang waktu arttikel dari tahun 2018 – 2022. Artikel yang dipilih adalah artikel eksperimental dan Studi Kasus. Kata kunci yang digunakan Holly Quran, Murotal Quran, Sleep Quality, Insomnia,Sleep Apneu, Elderley,Lansia. Dari database yang digunakan ditemukan 7 Artikel yang sesuai. Hasil dari penelitian yang didapat dari 7 artikel semua hasilnya menyatakan ada perbedaan yang signifikan pada kelompok yang mendapat intervemsi terapi murotal Al Quran pda kondisi pos terapi. Dibandingkan pre dan kelompok kontrolnya. Kesimpulannya Murotal Al Quran meningkatkan kualitas tidur pada Lansia.

Pengaruh Murottal Al-Qur’an Surah Ar Rahman Terhadap Kualitas Tidur Mahasiswa Fakultas Kedokteran Universitas Jember Musdalifana Vega Ratrinda; Komang Yunita Wiryaning Putri; Kristianningrum Dian Sofiana
Journal of Islamic Medicine Vol 7, No 1 (2023): JOURNAL OF ISLAMIC MEDICINE EDISI MARET 2023
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jim.v7i1.20817

Background:Medical students are at risk for experiencing sleep disturbances due to the demands of the academic assignments they face. Al-Quran murottal therapy is a sound therapy that is given to someone to have a relaxing effect Objectif : This study aims to determine the effect of murottal AlQuran on sleep quality of medical students, University of Jember Methods :This type of research is quasi experimental with one group pretest-postest design. The research was carried out for 1 week listen to the murotal Al Quran Surah Ar Rahman read by Qori Misyari Rasyid Al Afasy for 15 minutes at the respondent's location using the zoom application. The sample of this research was 18 students of medical student, University of Jember, class of 2018 and 2019. Samples were taken using the technique purposive sampling. Data were analyzed univariately and bivariately using testwilcoxon signed rank test and carried out the normality test using shapiro wilk Results: The results of the univariate analysis showed changes mean sleep quality pretest and posttest. Before listening to murottal Al-Quran have mean 8.16 and after listening to the Al-Quran to 3.66. The result of bivariate analysis is test wilcoxon signed rank test value is obtained p-value= 0.000 indicates that there is a significant difference between the provision of murottal Al-Quran and the sleep quality of medical students, University of Jember Conclusion : The conclusion of this study is that there is an effect of murottal AlQuran on improving the sleep quality of medical students, University of Jember

Pengaruh Murottal Al-Qur’an Surah Ar Rahman Terhadap Kualitas Tidur Mahasiswa Fakultas Kedokteran Universitas Jember Musdalifana Vega Ratrinda; Komang Yunita Wiryaning Putri; Kristianningrum Dian Sofiana
Journal of Islamic Medicine Vol 7, No 1 (2023): JOURNAL OF ISLAMIC MEDICINE EDISI MARET 2023
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jim.v7i1.20817

Background:Medical students are at risk for experiencing sleep disturbances due to the demands of the academic assignments they face. Al-Quran murottal therapy is a sound therapy that is given to someone to have a relaxing effect Objectif : This study aims to determine the effect of murottal AlQuran on sleep quality of medical students, University of Jember Methods :This type of research is quasi experimental with one group pretest-postest design. The research was carried out for 1 week listen to the murotal Al Quran Surah Ar Rahman read by Qori Misyari Rasyid Al Afasy for 15 minutes at the respondent's location using the zoom application. The sample of this research was 18 students of medical student, University of Jember, class of 2018 and 2019. Samples were taken using the technique purposive sampling. Data were analyzed univariately and bivariately using testwilcoxon signed rank test and carried out the normality test using shapiro wilk Results: The results of the univariate analysis showed changes mean sleep quality pretest and posttest. Before listening to murottal Al-Quran have mean 8.16 and after listening to the Al-Quran to 3.66. The result of bivariate analysis is test wilcoxon signed rank test value is obtained p-value= 0.000 indicates that there is a significant difference between the provision of murottal Al-Quran and the sleep quality of medical students, University of Jember Conclusion : The conclusion of this study is that there is an effect of murottal AlQuran on improving the sleep quality of medical students, University of Jember

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